Functional mapping of the activity of the R region in the human T-cell leukemia virus type I long terminal repeat to increase gene expression
Abstract We previously demonstrated the activity of the R fragment in the long terminal repeat of human T-cell leukemia virus type I for elevation of the level of gene expression. In this study, the fragment was deleted with BAL31 nuclease to determine its functional domain. Series of the shortened...
Ausführliche Beschreibung
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| Autor*in: |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1989 |
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| Umfang: |
9 |
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| Reproduktion: |
Springer Online Journal Archives 1860-2002 |
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| Übergeordnetes Werk: |
in: Virus genes - 1987, 2(1989) vom: Feb., Seite 147-155 |
| Übergeordnetes Werk: |
volume:2 ; year:1989 ; month:02 ; pages:147-155 ; extent:9 |
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NLEJ189978791 |
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| 245 | 1 | 0 | |a Functional mapping of the activity of the R region in the human T-cell leukemia virus type I long terminal repeat to increase gene expression |
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| 520 | |a Abstract We previously demonstrated the activity of the R fragment in the long terminal repeat of human T-cell leukemia virus type I for elevation of the level of gene expression. In this study, the fragment was deleted with BAL31 nuclease to determine its functional domain. Series of the shortened R fragments were linked to the simian virus 40 promoter unit, which regulated expression of a reporter gene. Examination with the R fragments deleted from the 5′ and 3′ ends showed that borders of the functional domain were mapped within nucleotide positions 458 to 473 for the 5′ end and nucleotide positions 559 to 594 for the 3′ end, respectively. Thus we conclude that a 136-base-pair fragment corresponding to the second half of the R region was sufficient to allow elevation of the level of gene expression. | ||
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| 700 | 1 | |a Ohtani, Kiyoshi |4 oth | |
| 700 | 1 | |a Hinuma, Yorio |4 oth | |
| 700 | 1 | |a Sugamura, Kazuo |4 oth | |
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(DE-627)NLEJ189978791 DE-627 ger DE-627 rakwb eng Functional mapping of the activity of the R region in the human T-cell leukemia virus type I long terminal repeat to increase gene expression 1989 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract We previously demonstrated the activity of the R fragment in the long terminal repeat of human T-cell leukemia virus type I for elevation of the level of gene expression. In this study, the fragment was deleted with BAL31 nuclease to determine its functional domain. Series of the shortened R fragments were linked to the simian virus 40 promoter unit, which regulated expression of a reporter gene. Examination with the R fragments deleted from the 5′ and 3′ ends showed that borders of the functional domain were mapped within nucleotide positions 458 to 473 for the 5′ end and nucleotide positions 559 to 594 for the 3′ end, respectively. Thus we conclude that a 136-base-pair fragment corresponding to the second half of the R region was sufficient to allow elevation of the level of gene expression. Springer Online Journal Archives 1860-2002 Nakamura, Masataka oth Ohtani, Kiyoshi oth Hinuma, Yorio oth Sugamura, Kazuo oth in Virus genes 1987 2(1989) vom: Feb., Seite 147-155 (DE-627)NLEJ188983716 (DE-600)2011138-1 1572-994X nnns volume:2 year:1989 month:02 pages:147-155 extent:9 http://dx.doi.org/10.1007/BF00315258 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 2 1989 2 147-155 9 |
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(DE-627)NLEJ189978791 DE-627 ger DE-627 rakwb eng Functional mapping of the activity of the R region in the human T-cell leukemia virus type I long terminal repeat to increase gene expression 1989 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract We previously demonstrated the activity of the R fragment in the long terminal repeat of human T-cell leukemia virus type I for elevation of the level of gene expression. In this study, the fragment was deleted with BAL31 nuclease to determine its functional domain. Series of the shortened R fragments were linked to the simian virus 40 promoter unit, which regulated expression of a reporter gene. Examination with the R fragments deleted from the 5′ and 3′ ends showed that borders of the functional domain were mapped within nucleotide positions 458 to 473 for the 5′ end and nucleotide positions 559 to 594 for the 3′ end, respectively. Thus we conclude that a 136-base-pair fragment corresponding to the second half of the R region was sufficient to allow elevation of the level of gene expression. Springer Online Journal Archives 1860-2002 Nakamura, Masataka oth Ohtani, Kiyoshi oth Hinuma, Yorio oth Sugamura, Kazuo oth in Virus genes 1987 2(1989) vom: Feb., Seite 147-155 (DE-627)NLEJ188983716 (DE-600)2011138-1 1572-994X nnns volume:2 year:1989 month:02 pages:147-155 extent:9 http://dx.doi.org/10.1007/BF00315258 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 2 1989 2 147-155 9 |
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(DE-627)NLEJ189978791 DE-627 ger DE-627 rakwb eng Functional mapping of the activity of the R region in the human T-cell leukemia virus type I long terminal repeat to increase gene expression 1989 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract We previously demonstrated the activity of the R fragment in the long terminal repeat of human T-cell leukemia virus type I for elevation of the level of gene expression. In this study, the fragment was deleted with BAL31 nuclease to determine its functional domain. Series of the shortened R fragments were linked to the simian virus 40 promoter unit, which regulated expression of a reporter gene. Examination with the R fragments deleted from the 5′ and 3′ ends showed that borders of the functional domain were mapped within nucleotide positions 458 to 473 for the 5′ end and nucleotide positions 559 to 594 for the 3′ end, respectively. Thus we conclude that a 136-base-pair fragment corresponding to the second half of the R region was sufficient to allow elevation of the level of gene expression. Springer Online Journal Archives 1860-2002 Nakamura, Masataka oth Ohtani, Kiyoshi oth Hinuma, Yorio oth Sugamura, Kazuo oth in Virus genes 1987 2(1989) vom: Feb., Seite 147-155 (DE-627)NLEJ188983716 (DE-600)2011138-1 1572-994X nnns volume:2 year:1989 month:02 pages:147-155 extent:9 http://dx.doi.org/10.1007/BF00315258 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 2 1989 2 147-155 9 |
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(DE-627)NLEJ189978791 DE-627 ger DE-627 rakwb eng Functional mapping of the activity of the R region in the human T-cell leukemia virus type I long terminal repeat to increase gene expression 1989 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract We previously demonstrated the activity of the R fragment in the long terminal repeat of human T-cell leukemia virus type I for elevation of the level of gene expression. In this study, the fragment was deleted with BAL31 nuclease to determine its functional domain. Series of the shortened R fragments were linked to the simian virus 40 promoter unit, which regulated expression of a reporter gene. Examination with the R fragments deleted from the 5′ and 3′ ends showed that borders of the functional domain were mapped within nucleotide positions 458 to 473 for the 5′ end and nucleotide positions 559 to 594 for the 3′ end, respectively. Thus we conclude that a 136-base-pair fragment corresponding to the second half of the R region was sufficient to allow elevation of the level of gene expression. Springer Online Journal Archives 1860-2002 Nakamura, Masataka oth Ohtani, Kiyoshi oth Hinuma, Yorio oth Sugamura, Kazuo oth in Virus genes 1987 2(1989) vom: Feb., Seite 147-155 (DE-627)NLEJ188983716 (DE-600)2011138-1 1572-994X nnns volume:2 year:1989 month:02 pages:147-155 extent:9 http://dx.doi.org/10.1007/BF00315258 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 2 1989 2 147-155 9 |
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(DE-627)NLEJ189978791 DE-627 ger DE-627 rakwb eng Functional mapping of the activity of the R region in the human T-cell leukemia virus type I long terminal repeat to increase gene expression 1989 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract We previously demonstrated the activity of the R fragment in the long terminal repeat of human T-cell leukemia virus type I for elevation of the level of gene expression. In this study, the fragment was deleted with BAL31 nuclease to determine its functional domain. Series of the shortened R fragments were linked to the simian virus 40 promoter unit, which regulated expression of a reporter gene. Examination with the R fragments deleted from the 5′ and 3′ ends showed that borders of the functional domain were mapped within nucleotide positions 458 to 473 for the 5′ end and nucleotide positions 559 to 594 for the 3′ end, respectively. Thus we conclude that a 136-base-pair fragment corresponding to the second half of the R region was sufficient to allow elevation of the level of gene expression. Springer Online Journal Archives 1860-2002 Nakamura, Masataka oth Ohtani, Kiyoshi oth Hinuma, Yorio oth Sugamura, Kazuo oth in Virus genes 1987 2(1989) vom: Feb., Seite 147-155 (DE-627)NLEJ188983716 (DE-600)2011138-1 1572-994X nnns volume:2 year:1989 month:02 pages:147-155 extent:9 http://dx.doi.org/10.1007/BF00315258 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 2 1989 2 147-155 9 |
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functional mapping of the activity of the r region in the human t-cell leukemia virus type i long terminal repeat to increase gene expression |
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Functional mapping of the activity of the R region in the human T-cell leukemia virus type I long terminal repeat to increase gene expression |
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Abstract We previously demonstrated the activity of the R fragment in the long terminal repeat of human T-cell leukemia virus type I for elevation of the level of gene expression. In this study, the fragment was deleted with BAL31 nuclease to determine its functional domain. Series of the shortened R fragments were linked to the simian virus 40 promoter unit, which regulated expression of a reporter gene. Examination with the R fragments deleted from the 5′ and 3′ ends showed that borders of the functional domain were mapped within nucleotide positions 458 to 473 for the 5′ end and nucleotide positions 559 to 594 for the 3′ end, respectively. Thus we conclude that a 136-base-pair fragment corresponding to the second half of the R region was sufficient to allow elevation of the level of gene expression. |
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Abstract We previously demonstrated the activity of the R fragment in the long terminal repeat of human T-cell leukemia virus type I for elevation of the level of gene expression. In this study, the fragment was deleted with BAL31 nuclease to determine its functional domain. Series of the shortened R fragments were linked to the simian virus 40 promoter unit, which regulated expression of a reporter gene. Examination with the R fragments deleted from the 5′ and 3′ ends showed that borders of the functional domain were mapped within nucleotide positions 458 to 473 for the 5′ end and nucleotide positions 559 to 594 for the 3′ end, respectively. Thus we conclude that a 136-base-pair fragment corresponding to the second half of the R region was sufficient to allow elevation of the level of gene expression. |
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Abstract We previously demonstrated the activity of the R fragment in the long terminal repeat of human T-cell leukemia virus type I for elevation of the level of gene expression. In this study, the fragment was deleted with BAL31 nuclease to determine its functional domain. Series of the shortened R fragments were linked to the simian virus 40 promoter unit, which regulated expression of a reporter gene. Examination with the R fragments deleted from the 5′ and 3′ ends showed that borders of the functional domain were mapped within nucleotide positions 458 to 473 for the 5′ end and nucleotide positions 559 to 594 for the 3′ end, respectively. Thus we conclude that a 136-base-pair fragment corresponding to the second half of the R region was sufficient to allow elevation of the level of gene expression. |
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