Evaluation of laboratory diagnosis of taxoplasmosis by means of an ELISA-triple test
Abstract The combination of three parameters (IgG, IgM and circulating antigen) in a so-called ‘ELISA triple test’ was suggested for advantageous diagnosing of human toxoplasmosis. A qualitative assay was used with the following arbitrary assumptions: IgG antibodies reflect an infection, IgM antibod...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1986 |
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| Umfang: |
9 |
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| Reproduktion: |
Springer Online Journal Archives 1860-2002 |
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| Übergeordnetes Werk: |
in: Antonie van Leeuwenhoek - 1934, 52(1986) vom: Jan., Seite 5-13 |
| Übergeordnetes Werk: |
volume:52 ; year:1986 ; month:01 ; pages:5-13 ; extent:9 |
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NLEJ192925253 |
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| 520 | |a Abstract The combination of three parameters (IgG, IgM and circulating antigen) in a so-called ‘ELISA triple test’ was suggested for advantageous diagnosing of human toxoplasmosis. A qualitative assay was used with the following arbitrary assumptions: IgG antibodies reflect an infection, IgM antibodies reflect a recent (primary) infection and circulating antigens reflect an active infection. The three assays were performed simultaneously in one microtiter plate. This approach was tested with 1091 patientsera submitted for routine diagnosis. In comparison with conventional indirect immunofluorescence and complement fixation test it was observed that combinations indicating a recently aquired infection (combinations with IgM and/or circulating antigen) mainly paralleled low or negligible conventional antibody titers. No strict association was seen between particular combinations and certain clinical symptoms suggestive for toxoplasmosis. In conclusion it was stated that the triple test for support of clinical diagnosis has some advantages but that a strong need exists to be able to demonstrate exacerbation or reinfections which are generally not characterised by IgM antibody formation of free circulating antigens but may be the reason for elevated conventional antibody titers. | ||
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(DE-627)NLEJ192925253 DE-627 ger DE-627 rakwb eng Evaluation of laboratory diagnosis of taxoplasmosis by means of an ELISA-triple test 1986 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract The combination of three parameters (IgG, IgM and circulating antigen) in a so-called ‘ELISA triple test’ was suggested for advantageous diagnosing of human toxoplasmosis. A qualitative assay was used with the following arbitrary assumptions: IgG antibodies reflect an infection, IgM antibodies reflect a recent (primary) infection and circulating antigens reflect an active infection. The three assays were performed simultaneously in one microtiter plate. This approach was tested with 1091 patientsera submitted for routine diagnosis. In comparison with conventional indirect immunofluorescence and complement fixation test it was observed that combinations indicating a recently aquired infection (combinations with IgM and/or circulating antigen) mainly paralleled low or negligible conventional antibody titers. No strict association was seen between particular combinations and certain clinical symptoms suggestive for toxoplasmosis. In conclusion it was stated that the triple test for support of clinical diagnosis has some advantages but that a strong need exists to be able to demonstrate exacerbation or reinfections which are generally not characterised by IgM antibody formation of free circulating antigens but may be the reason for elevated conventional antibody titers. Springer Online Journal Archives 1860-2002 Knapen, F. oth Panggabean, S. O. oth Leusden, J. oth in Antonie van Leeuwenhoek 1934 52(1986) vom: Jan., Seite 5-13 (DE-627)NLEJ188983511 (DE-600)1478112-8 1572-9699 nnns volume:52 year:1986 month:01 pages:5-13 extent:9 http://dx.doi.org/10.1007/BF00402682 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 52 1986 1 5-13 9 |
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(DE-627)NLEJ192925253 DE-627 ger DE-627 rakwb eng Evaluation of laboratory diagnosis of taxoplasmosis by means of an ELISA-triple test 1986 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract The combination of three parameters (IgG, IgM and circulating antigen) in a so-called ‘ELISA triple test’ was suggested for advantageous diagnosing of human toxoplasmosis. A qualitative assay was used with the following arbitrary assumptions: IgG antibodies reflect an infection, IgM antibodies reflect a recent (primary) infection and circulating antigens reflect an active infection. The three assays were performed simultaneously in one microtiter plate. This approach was tested with 1091 patientsera submitted for routine diagnosis. In comparison with conventional indirect immunofluorescence and complement fixation test it was observed that combinations indicating a recently aquired infection (combinations with IgM and/or circulating antigen) mainly paralleled low or negligible conventional antibody titers. No strict association was seen between particular combinations and certain clinical symptoms suggestive for toxoplasmosis. In conclusion it was stated that the triple test for support of clinical diagnosis has some advantages but that a strong need exists to be able to demonstrate exacerbation or reinfections which are generally not characterised by IgM antibody formation of free circulating antigens but may be the reason for elevated conventional antibody titers. Springer Online Journal Archives 1860-2002 Knapen, F. oth Panggabean, S. O. oth Leusden, J. oth in Antonie van Leeuwenhoek 1934 52(1986) vom: Jan., Seite 5-13 (DE-627)NLEJ188983511 (DE-600)1478112-8 1572-9699 nnns volume:52 year:1986 month:01 pages:5-13 extent:9 http://dx.doi.org/10.1007/BF00402682 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 52 1986 1 5-13 9 |
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(DE-627)NLEJ192925253 DE-627 ger DE-627 rakwb eng Evaluation of laboratory diagnosis of taxoplasmosis by means of an ELISA-triple test 1986 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract The combination of three parameters (IgG, IgM and circulating antigen) in a so-called ‘ELISA triple test’ was suggested for advantageous diagnosing of human toxoplasmosis. A qualitative assay was used with the following arbitrary assumptions: IgG antibodies reflect an infection, IgM antibodies reflect a recent (primary) infection and circulating antigens reflect an active infection. The three assays were performed simultaneously in one microtiter plate. This approach was tested with 1091 patientsera submitted for routine diagnosis. In comparison with conventional indirect immunofluorescence and complement fixation test it was observed that combinations indicating a recently aquired infection (combinations with IgM and/or circulating antigen) mainly paralleled low or negligible conventional antibody titers. No strict association was seen between particular combinations and certain clinical symptoms suggestive for toxoplasmosis. In conclusion it was stated that the triple test for support of clinical diagnosis has some advantages but that a strong need exists to be able to demonstrate exacerbation or reinfections which are generally not characterised by IgM antibody formation of free circulating antigens but may be the reason for elevated conventional antibody titers. Springer Online Journal Archives 1860-2002 Knapen, F. oth Panggabean, S. O. oth Leusden, J. oth in Antonie van Leeuwenhoek 1934 52(1986) vom: Jan., Seite 5-13 (DE-627)NLEJ188983511 (DE-600)1478112-8 1572-9699 nnns volume:52 year:1986 month:01 pages:5-13 extent:9 http://dx.doi.org/10.1007/BF00402682 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 52 1986 1 5-13 9 |
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(DE-627)NLEJ192925253 DE-627 ger DE-627 rakwb eng Evaluation of laboratory diagnosis of taxoplasmosis by means of an ELISA-triple test 1986 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract The combination of three parameters (IgG, IgM and circulating antigen) in a so-called ‘ELISA triple test’ was suggested for advantageous diagnosing of human toxoplasmosis. A qualitative assay was used with the following arbitrary assumptions: IgG antibodies reflect an infection, IgM antibodies reflect a recent (primary) infection and circulating antigens reflect an active infection. The three assays were performed simultaneously in one microtiter plate. This approach was tested with 1091 patientsera submitted for routine diagnosis. In comparison with conventional indirect immunofluorescence and complement fixation test it was observed that combinations indicating a recently aquired infection (combinations with IgM and/or circulating antigen) mainly paralleled low or negligible conventional antibody titers. No strict association was seen between particular combinations and certain clinical symptoms suggestive for toxoplasmosis. In conclusion it was stated that the triple test for support of clinical diagnosis has some advantages but that a strong need exists to be able to demonstrate exacerbation or reinfections which are generally not characterised by IgM antibody formation of free circulating antigens but may be the reason for elevated conventional antibody titers. Springer Online Journal Archives 1860-2002 Knapen, F. oth Panggabean, S. O. oth Leusden, J. oth in Antonie van Leeuwenhoek 1934 52(1986) vom: Jan., Seite 5-13 (DE-627)NLEJ188983511 (DE-600)1478112-8 1572-9699 nnns volume:52 year:1986 month:01 pages:5-13 extent:9 http://dx.doi.org/10.1007/BF00402682 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 52 1986 1 5-13 9 |
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(DE-627)NLEJ192925253 DE-627 ger DE-627 rakwb eng Evaluation of laboratory diagnosis of taxoplasmosis by means of an ELISA-triple test 1986 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract The combination of three parameters (IgG, IgM and circulating antigen) in a so-called ‘ELISA triple test’ was suggested for advantageous diagnosing of human toxoplasmosis. A qualitative assay was used with the following arbitrary assumptions: IgG antibodies reflect an infection, IgM antibodies reflect a recent (primary) infection and circulating antigens reflect an active infection. The three assays were performed simultaneously in one microtiter plate. This approach was tested with 1091 patientsera submitted for routine diagnosis. In comparison with conventional indirect immunofluorescence and complement fixation test it was observed that combinations indicating a recently aquired infection (combinations with IgM and/or circulating antigen) mainly paralleled low or negligible conventional antibody titers. No strict association was seen between particular combinations and certain clinical symptoms suggestive for toxoplasmosis. In conclusion it was stated that the triple test for support of clinical diagnosis has some advantages but that a strong need exists to be able to demonstrate exacerbation or reinfections which are generally not characterised by IgM antibody formation of free circulating antigens but may be the reason for elevated conventional antibody titers. Springer Online Journal Archives 1860-2002 Knapen, F. oth Panggabean, S. O. oth Leusden, J. oth in Antonie van Leeuwenhoek 1934 52(1986) vom: Jan., Seite 5-13 (DE-627)NLEJ188983511 (DE-600)1478112-8 1572-9699 nnns volume:52 year:1986 month:01 pages:5-13 extent:9 http://dx.doi.org/10.1007/BF00402682 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 52 1986 1 5-13 9 |
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Evaluation of laboratory diagnosis of taxoplasmosis by means of an ELISA-triple test |
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Abstract The combination of three parameters (IgG, IgM and circulating antigen) in a so-called ‘ELISA triple test’ was suggested for advantageous diagnosing of human toxoplasmosis. A qualitative assay was used with the following arbitrary assumptions: IgG antibodies reflect an infection, IgM antibodies reflect a recent (primary) infection and circulating antigens reflect an active infection. The three assays were performed simultaneously in one microtiter plate. This approach was tested with 1091 patientsera submitted for routine diagnosis. In comparison with conventional indirect immunofluorescence and complement fixation test it was observed that combinations indicating a recently aquired infection (combinations with IgM and/or circulating antigen) mainly paralleled low or negligible conventional antibody titers. No strict association was seen between particular combinations and certain clinical symptoms suggestive for toxoplasmosis. In conclusion it was stated that the triple test for support of clinical diagnosis has some advantages but that a strong need exists to be able to demonstrate exacerbation or reinfections which are generally not characterised by IgM antibody formation of free circulating antigens but may be the reason for elevated conventional antibody titers. |
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Abstract The combination of three parameters (IgG, IgM and circulating antigen) in a so-called ‘ELISA triple test’ was suggested for advantageous diagnosing of human toxoplasmosis. A qualitative assay was used with the following arbitrary assumptions: IgG antibodies reflect an infection, IgM antibodies reflect a recent (primary) infection and circulating antigens reflect an active infection. The three assays were performed simultaneously in one microtiter plate. This approach was tested with 1091 patientsera submitted for routine diagnosis. In comparison with conventional indirect immunofluorescence and complement fixation test it was observed that combinations indicating a recently aquired infection (combinations with IgM and/or circulating antigen) mainly paralleled low or negligible conventional antibody titers. No strict association was seen between particular combinations and certain clinical symptoms suggestive for toxoplasmosis. In conclusion it was stated that the triple test for support of clinical diagnosis has some advantages but that a strong need exists to be able to demonstrate exacerbation or reinfections which are generally not characterised by IgM antibody formation of free circulating antigens but may be the reason for elevated conventional antibody titers. |
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Abstract The combination of three parameters (IgG, IgM and circulating antigen) in a so-called ‘ELISA triple test’ was suggested for advantageous diagnosing of human toxoplasmosis. A qualitative assay was used with the following arbitrary assumptions: IgG antibodies reflect an infection, IgM antibodies reflect a recent (primary) infection and circulating antigens reflect an active infection. The three assays were performed simultaneously in one microtiter plate. This approach was tested with 1091 patientsera submitted for routine diagnosis. In comparison with conventional indirect immunofluorescence and complement fixation test it was observed that combinations indicating a recently aquired infection (combinations with IgM and/or circulating antigen) mainly paralleled low or negligible conventional antibody titers. No strict association was seen between particular combinations and certain clinical symptoms suggestive for toxoplasmosis. In conclusion it was stated that the triple test for support of clinical diagnosis has some advantages but that a strong need exists to be able to demonstrate exacerbation or reinfections which are generally not characterised by IgM antibody formation of free circulating antigens but may be the reason for elevated conventional antibody titers. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ192925253</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230505194055.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070526s1986 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ192925253</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Evaluation of laboratory diagnosis of taxoplasmosis by means of an ELISA-triple test</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1986</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">9</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract The combination of three parameters (IgG, IgM and circulating antigen) in a so-called ‘ELISA triple test’ was suggested for advantageous diagnosing of human toxoplasmosis. A qualitative assay was used with the following arbitrary assumptions: IgG antibodies reflect an infection, IgM antibodies reflect a recent (primary) infection and circulating antigens reflect an active infection. The three assays were performed simultaneously in one microtiter plate. This approach was tested with 1091 patientsera submitted for routine diagnosis. In comparison with conventional indirect immunofluorescence and complement fixation test it was observed that combinations indicating a recently aquired infection (combinations with IgM and/or circulating antigen) mainly paralleled low or negligible conventional antibody titers. No strict association was seen between particular combinations and certain clinical symptoms suggestive for toxoplasmosis. In conclusion it was stated that the triple test for support of clinical diagnosis has some advantages but that a strong need exists to be able to demonstrate exacerbation or reinfections which are generally not characterised by IgM antibody formation of free circulating antigens but may be the reason for elevated conventional antibody titers.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Springer Online Journal Archives 1860-2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Knapen, F.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Panggabean, S. O.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Leusden, J.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">Antonie van Leeuwenhoek</subfield><subfield code="d">1934</subfield><subfield code="g">52(1986) vom: Jan., Seite 5-13</subfield><subfield code="w">(DE-627)NLEJ188983511</subfield><subfield code="w">(DE-600)1478112-8</subfield><subfield code="x">1572-9699</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:52</subfield><subfield code="g">year:1986</subfield><subfield code="g">month:01</subfield><subfield code="g">pages:5-13</subfield><subfield code="g">extent:9</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1007/BF00402682</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SOJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">52</subfield><subfield code="j">1986</subfield><subfield code="c">1</subfield><subfield code="h">5-13</subfield><subfield code="g">9</subfield></datafield></record></collection>
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