A review of available systems to investigate transfer of DNA to indigenous soil bacteria
Abstract The deliberate or accidental release of genetically engineered microorganisms (GEMs) in the environment has led to some questions concerning microbial survival, transfer of DNA to the indigenous microflora and environmental consequences. Amongst horizontal gene transfer mechanisms, conjugat...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1998 |
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| Umfang: |
9 |
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| Reproduktion: |
Springer Online Journal Archives 1860-2002 |
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| Übergeordnetes Werk: |
in: Antonie van Leeuwenhoek - 1934, 73(1998) vom: Jan., Seite 15-23 |
| Übergeordnetes Werk: |
volume:73 ; year:1998 ; month:01 ; pages:15-23 ; extent:9 |
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NLEJ192934872 |
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| 520 | |a Abstract The deliberate or accidental release of genetically engineered microorganisms (GEMs) in the environment has led to some questions concerning microbial survival, transfer of DNA to the indigenous microflora and environmental consequences. Amongst horizontal gene transfer mechanisms, conjugation is probably the most frequent in the environment. With the aim of evaluating risks associated with environmental release of GEMs and their engineered DNA, studies of conjugative gene transfer between a donor strain and indigenous microflora have been conducted. Such studies required the development of a donor counterselection system to prevent growth of donor cells on transconjugant selective plates. This review summarizes the known and potential donor counterselection systems. | ||
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(DE-627)NLEJ192934872 DE-627 ger DE-627 rakwb eng A review of available systems to investigate transfer of DNA to indigenous soil bacteria 1998 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract The deliberate or accidental release of genetically engineered microorganisms (GEMs) in the environment has led to some questions concerning microbial survival, transfer of DNA to the indigenous microflora and environmental consequences. Amongst horizontal gene transfer mechanisms, conjugation is probably the most frequent in the environment. With the aim of evaluating risks associated with environmental release of GEMs and their engineered DNA, studies of conjugative gene transfer between a donor strain and indigenous microflora have been conducted. Such studies required the development of a donor counterselection system to prevent growth of donor cells on transconjugant selective plates. This review summarizes the known and potential donor counterselection systems. Springer Online Journal Archives 1860-2002 Clerc, Sylvie oth Simonet, Pascal oth in Antonie van Leeuwenhoek 1934 73(1998) vom: Jan., Seite 15-23 (DE-627)NLEJ188983511 (DE-600)1478112-8 1572-9699 nnns volume:73 year:1998 month:01 pages:15-23 extent:9 http://dx.doi.org/10.1023/A:1000747310461 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 73 1998 1 15-23 9 |
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(DE-627)NLEJ192934872 DE-627 ger DE-627 rakwb eng A review of available systems to investigate transfer of DNA to indigenous soil bacteria 1998 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract The deliberate or accidental release of genetically engineered microorganisms (GEMs) in the environment has led to some questions concerning microbial survival, transfer of DNA to the indigenous microflora and environmental consequences. Amongst horizontal gene transfer mechanisms, conjugation is probably the most frequent in the environment. With the aim of evaluating risks associated with environmental release of GEMs and their engineered DNA, studies of conjugative gene transfer between a donor strain and indigenous microflora have been conducted. Such studies required the development of a donor counterselection system to prevent growth of donor cells on transconjugant selective plates. This review summarizes the known and potential donor counterselection systems. Springer Online Journal Archives 1860-2002 Clerc, Sylvie oth Simonet, Pascal oth in Antonie van Leeuwenhoek 1934 73(1998) vom: Jan., Seite 15-23 (DE-627)NLEJ188983511 (DE-600)1478112-8 1572-9699 nnns volume:73 year:1998 month:01 pages:15-23 extent:9 http://dx.doi.org/10.1023/A:1000747310461 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 73 1998 1 15-23 9 |
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(DE-627)NLEJ192934872 DE-627 ger DE-627 rakwb eng A review of available systems to investigate transfer of DNA to indigenous soil bacteria 1998 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract The deliberate or accidental release of genetically engineered microorganisms (GEMs) in the environment has led to some questions concerning microbial survival, transfer of DNA to the indigenous microflora and environmental consequences. Amongst horizontal gene transfer mechanisms, conjugation is probably the most frequent in the environment. With the aim of evaluating risks associated with environmental release of GEMs and their engineered DNA, studies of conjugative gene transfer between a donor strain and indigenous microflora have been conducted. Such studies required the development of a donor counterselection system to prevent growth of donor cells on transconjugant selective plates. This review summarizes the known and potential donor counterselection systems. Springer Online Journal Archives 1860-2002 Clerc, Sylvie oth Simonet, Pascal oth in Antonie van Leeuwenhoek 1934 73(1998) vom: Jan., Seite 15-23 (DE-627)NLEJ188983511 (DE-600)1478112-8 1572-9699 nnns volume:73 year:1998 month:01 pages:15-23 extent:9 http://dx.doi.org/10.1023/A:1000747310461 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 73 1998 1 15-23 9 |
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(DE-627)NLEJ192934872 DE-627 ger DE-627 rakwb eng A review of available systems to investigate transfer of DNA to indigenous soil bacteria 1998 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract The deliberate or accidental release of genetically engineered microorganisms (GEMs) in the environment has led to some questions concerning microbial survival, transfer of DNA to the indigenous microflora and environmental consequences. Amongst horizontal gene transfer mechanisms, conjugation is probably the most frequent in the environment. With the aim of evaluating risks associated with environmental release of GEMs and their engineered DNA, studies of conjugative gene transfer between a donor strain and indigenous microflora have been conducted. Such studies required the development of a donor counterselection system to prevent growth of donor cells on transconjugant selective plates. This review summarizes the known and potential donor counterselection systems. Springer Online Journal Archives 1860-2002 Clerc, Sylvie oth Simonet, Pascal oth in Antonie van Leeuwenhoek 1934 73(1998) vom: Jan., Seite 15-23 (DE-627)NLEJ188983511 (DE-600)1478112-8 1572-9699 nnns volume:73 year:1998 month:01 pages:15-23 extent:9 http://dx.doi.org/10.1023/A:1000747310461 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 73 1998 1 15-23 9 |
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(DE-627)NLEJ192934872 DE-627 ger DE-627 rakwb eng A review of available systems to investigate transfer of DNA to indigenous soil bacteria 1998 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract The deliberate or accidental release of genetically engineered microorganisms (GEMs) in the environment has led to some questions concerning microbial survival, transfer of DNA to the indigenous microflora and environmental consequences. Amongst horizontal gene transfer mechanisms, conjugation is probably the most frequent in the environment. With the aim of evaluating risks associated with environmental release of GEMs and their engineered DNA, studies of conjugative gene transfer between a donor strain and indigenous microflora have been conducted. Such studies required the development of a donor counterselection system to prevent growth of donor cells on transconjugant selective plates. This review summarizes the known and potential donor counterselection systems. Springer Online Journal Archives 1860-2002 Clerc, Sylvie oth Simonet, Pascal oth in Antonie van Leeuwenhoek 1934 73(1998) vom: Jan., Seite 15-23 (DE-627)NLEJ188983511 (DE-600)1478112-8 1572-9699 nnns volume:73 year:1998 month:01 pages:15-23 extent:9 http://dx.doi.org/10.1023/A:1000747310461 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 73 1998 1 15-23 9 |
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A review of available systems to investigate transfer of DNA to indigenous soil bacteria |
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Abstract The deliberate or accidental release of genetically engineered microorganisms (GEMs) in the environment has led to some questions concerning microbial survival, transfer of DNA to the indigenous microflora and environmental consequences. Amongst horizontal gene transfer mechanisms, conjugation is probably the most frequent in the environment. With the aim of evaluating risks associated with environmental release of GEMs and their engineered DNA, studies of conjugative gene transfer between a donor strain and indigenous microflora have been conducted. Such studies required the development of a donor counterselection system to prevent growth of donor cells on transconjugant selective plates. This review summarizes the known and potential donor counterselection systems. |
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Abstract The deliberate or accidental release of genetically engineered microorganisms (GEMs) in the environment has led to some questions concerning microbial survival, transfer of DNA to the indigenous microflora and environmental consequences. Amongst horizontal gene transfer mechanisms, conjugation is probably the most frequent in the environment. With the aim of evaluating risks associated with environmental release of GEMs and their engineered DNA, studies of conjugative gene transfer between a donor strain and indigenous microflora have been conducted. Such studies required the development of a donor counterselection system to prevent growth of donor cells on transconjugant selective plates. This review summarizes the known and potential donor counterselection systems. |
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Abstract The deliberate or accidental release of genetically engineered microorganisms (GEMs) in the environment has led to some questions concerning microbial survival, transfer of DNA to the indigenous microflora and environmental consequences. Amongst horizontal gene transfer mechanisms, conjugation is probably the most frequent in the environment. With the aim of evaluating risks associated with environmental release of GEMs and their engineered DNA, studies of conjugative gene transfer between a donor strain and indigenous microflora have been conducted. Such studies required the development of a donor counterselection system to prevent growth of donor cells on transconjugant selective plates. This review summarizes the known and potential donor counterselection systems. |
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