A highly efficient electroporation system for transformation of Bacillus licheniformis
Summary Transformation of intact cells of Bacillus licheniformis 5A24 with plasmids pLM6 (2.8 kb), pC194 (2.9 kb) and pCP49 (7.1 kb) by electroporation resulted in 1.5 × 106, 1.2 × 106 , and 5.2 × 104 transformants per μg DNA, respectively. Transformation...
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| Autor*in: |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1991 |
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| Umfang: |
4 |
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| Reproduktion: |
Springer Online Journal Archives 1860-2002 |
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| Übergeordnetes Werk: |
in: Biotechnology techniques - 1987, 5(1991) vom: Jan., Seite 5-8 |
| Übergeordnetes Werk: |
volume:5 ; year:1991 ; month:01 ; pages:5-8 ; extent:4 |
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| 520 | |a Summary Transformation of intact cells of Bacillus licheniformis 5A24 with plasmids pLM6 (2.8 kb), pC194 (2.9 kb) and pCP49 (7.1 kb) by electroporation resulted in 1.5 × 106, 1.2 × 106 , and 5.2 × 104 transformants per μg DNA, respectively. Transformation was possible with plasmid DNA, which was religated after restriction endonuclease digestions. In addition, evidence is presented that indicates that B. licheniformis possesses DNA restriction and modification systems. | ||
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| 700 | 1 | |a Brigidi, Patrizia |4 oth | |
| 700 | 1 | |a Rossi, Edda |4 oth | |
| 700 | 1 | |a Riccardi, Giovanna |4 oth | |
| 700 | 1 | |a Matteuzzi, Diego |4 oth | |
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(DE-627)NLEJ19341760X DE-627 ger DE-627 rakwb eng A highly efficient electroporation system for transformation of Bacillus licheniformis 1991 4 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary Transformation of intact cells of Bacillus licheniformis 5A24 with plasmids pLM6 (2.8 kb), pC194 (2.9 kb) and pCP49 (7.1 kb) by electroporation resulted in 1.5 × 106, 1.2 × 106 , and 5.2 × 104 transformants per μg DNA, respectively. Transformation was possible with plasmid DNA, which was religated after restriction endonuclease digestions. In addition, evidence is presented that indicates that B. licheniformis possesses DNA restriction and modification systems. Springer Online Journal Archives 1860-2002 Brigidi, Patrizia oth Rossi, Edda oth Riccardi, Giovanna oth Matteuzzi, Diego oth in Biotechnology techniques 1987 5(1991) vom: Jan., Seite 5-8 (DE-627)NLEJ188983945 (DE-600)2015000-3 1573-6784 nnns volume:5 year:1991 month:01 pages:5-8 extent:4 http://dx.doi.org/10.1007/BF00152745 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 5 1991 1 5-8 4 |
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(DE-627)NLEJ19341760X DE-627 ger DE-627 rakwb eng A highly efficient electroporation system for transformation of Bacillus licheniformis 1991 4 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary Transformation of intact cells of Bacillus licheniformis 5A24 with plasmids pLM6 (2.8 kb), pC194 (2.9 kb) and pCP49 (7.1 kb) by electroporation resulted in 1.5 × 106, 1.2 × 106 , and 5.2 × 104 transformants per μg DNA, respectively. Transformation was possible with plasmid DNA, which was religated after restriction endonuclease digestions. In addition, evidence is presented that indicates that B. licheniformis possesses DNA restriction and modification systems. Springer Online Journal Archives 1860-2002 Brigidi, Patrizia oth Rossi, Edda oth Riccardi, Giovanna oth Matteuzzi, Diego oth in Biotechnology techniques 1987 5(1991) vom: Jan., Seite 5-8 (DE-627)NLEJ188983945 (DE-600)2015000-3 1573-6784 nnns volume:5 year:1991 month:01 pages:5-8 extent:4 http://dx.doi.org/10.1007/BF00152745 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 5 1991 1 5-8 4 |
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(DE-627)NLEJ19341760X DE-627 ger DE-627 rakwb eng A highly efficient electroporation system for transformation of Bacillus licheniformis 1991 4 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary Transformation of intact cells of Bacillus licheniformis 5A24 with plasmids pLM6 (2.8 kb), pC194 (2.9 kb) and pCP49 (7.1 kb) by electroporation resulted in 1.5 × 106, 1.2 × 106 , and 5.2 × 104 transformants per μg DNA, respectively. Transformation was possible with plasmid DNA, which was religated after restriction endonuclease digestions. In addition, evidence is presented that indicates that B. licheniformis possesses DNA restriction and modification systems. Springer Online Journal Archives 1860-2002 Brigidi, Patrizia oth Rossi, Edda oth Riccardi, Giovanna oth Matteuzzi, Diego oth in Biotechnology techniques 1987 5(1991) vom: Jan., Seite 5-8 (DE-627)NLEJ188983945 (DE-600)2015000-3 1573-6784 nnns volume:5 year:1991 month:01 pages:5-8 extent:4 http://dx.doi.org/10.1007/BF00152745 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 5 1991 1 5-8 4 |
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(DE-627)NLEJ19341760X DE-627 ger DE-627 rakwb eng A highly efficient electroporation system for transformation of Bacillus licheniformis 1991 4 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary Transformation of intact cells of Bacillus licheniformis 5A24 with plasmids pLM6 (2.8 kb), pC194 (2.9 kb) and pCP49 (7.1 kb) by electroporation resulted in 1.5 × 106, 1.2 × 106 , and 5.2 × 104 transformants per μg DNA, respectively. Transformation was possible with plasmid DNA, which was religated after restriction endonuclease digestions. In addition, evidence is presented that indicates that B. licheniformis possesses DNA restriction and modification systems. Springer Online Journal Archives 1860-2002 Brigidi, Patrizia oth Rossi, Edda oth Riccardi, Giovanna oth Matteuzzi, Diego oth in Biotechnology techniques 1987 5(1991) vom: Jan., Seite 5-8 (DE-627)NLEJ188983945 (DE-600)2015000-3 1573-6784 nnns volume:5 year:1991 month:01 pages:5-8 extent:4 http://dx.doi.org/10.1007/BF00152745 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 5 1991 1 5-8 4 |
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(DE-627)NLEJ19341760X DE-627 ger DE-627 rakwb eng A highly efficient electroporation system for transformation of Bacillus licheniformis 1991 4 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary Transformation of intact cells of Bacillus licheniformis 5A24 with plasmids pLM6 (2.8 kb), pC194 (2.9 kb) and pCP49 (7.1 kb) by electroporation resulted in 1.5 × 106, 1.2 × 106 , and 5.2 × 104 transformants per μg DNA, respectively. Transformation was possible with plasmid DNA, which was religated after restriction endonuclease digestions. In addition, evidence is presented that indicates that B. licheniformis possesses DNA restriction and modification systems. Springer Online Journal Archives 1860-2002 Brigidi, Patrizia oth Rossi, Edda oth Riccardi, Giovanna oth Matteuzzi, Diego oth in Biotechnology techniques 1987 5(1991) vom: Jan., Seite 5-8 (DE-627)NLEJ188983945 (DE-600)2015000-3 1573-6784 nnns volume:5 year:1991 month:01 pages:5-8 extent:4 http://dx.doi.org/10.1007/BF00152745 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 5 1991 1 5-8 4 |
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a highly efficient electroporation system for transformation of bacillus licheniformis |
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A highly efficient electroporation system for transformation of Bacillus licheniformis |
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Summary Transformation of intact cells of Bacillus licheniformis 5A24 with plasmids pLM6 (2.8 kb), pC194 (2.9 kb) and pCP49 (7.1 kb) by electroporation resulted in 1.5 × 106, 1.2 × 106 , and 5.2 × 104 transformants per μg DNA, respectively. Transformation was possible with plasmid DNA, which was religated after restriction endonuclease digestions. In addition, evidence is presented that indicates that B. licheniformis possesses DNA restriction and modification systems. |
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Summary Transformation of intact cells of Bacillus licheniformis 5A24 with plasmids pLM6 (2.8 kb), pC194 (2.9 kb) and pCP49 (7.1 kb) by electroporation resulted in 1.5 × 106, 1.2 × 106 , and 5.2 × 104 transformants per μg DNA, respectively. Transformation was possible with plasmid DNA, which was religated after restriction endonuclease digestions. In addition, evidence is presented that indicates that B. licheniformis possesses DNA restriction and modification systems. |
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Summary Transformation of intact cells of Bacillus licheniformis 5A24 with plasmids pLM6 (2.8 kb), pC194 (2.9 kb) and pCP49 (7.1 kb) by electroporation resulted in 1.5 × 106, 1.2 × 106 , and 5.2 × 104 transformants per μg DNA, respectively. Transformation was possible with plasmid DNA, which was religated after restriction endonuclease digestions. In addition, evidence is presented that indicates that B. licheniformis possesses DNA restriction and modification systems. |
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