Effect of cell isolation methods and drug concentration on the use of the Differential Staining Cytotoxicity (DiSC) assay with solid tumours
Abstract The Differential Staining Cytotoxicity (DiSC) in vitro drug sensitivity assay has been tested in solid tumours obtained from surgery. Various enzyme cocktails have been used for disaggregating cells for times ranging from 1–72 h. Collagenase (0.5%) plus DNase (0.002%) was chosen as producin...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1989 |
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| Umfang: |
8 |
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| Reproduktion: |
Springer Online Journal Archives 1860-2002 |
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| Übergeordnetes Werk: |
in: Cytotechnology - 1987, 2(1989) vom: März, Seite 225-232 |
| Übergeordnetes Werk: |
volume:2 ; year:1989 ; month:03 ; pages:225-232 ; extent:8 |
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NLEJ193656949 |
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| 520 | |a Abstract The Differential Staining Cytotoxicity (DiSC) in vitro drug sensitivity assay has been tested in solid tumours obtained from surgery. Various enzyme cocktails have been used for disaggregating cells for times ranging from 1–72 h. Collagenase (0.5%) plus DNase (0.002%) was chosen as producing the best results when incubated for 3 h at 37°C. This cocktail was also used for 24, 48 and 72 h incubations. Cell suspensions so produced were sometimes cleaned up by centrifugation on a Nycodenz gradient. Breast tumours were more often successfully cultured (67%) compared to gastrointestinal (43%) or other tumours (34%). Results with 6 drugs tested in 10 or more tumours suggested that in general, higher drug concentrations should be used when testing solid tumours as opposed to leukaemias in the DiSC assay. | ||
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(DE-627)NLEJ193656949 DE-627 ger DE-627 rakwb eng Effect of cell isolation methods and drug concentration on the use of the Differential Staining Cytotoxicity (DiSC) assay with solid tumours 1989 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract The Differential Staining Cytotoxicity (DiSC) in vitro drug sensitivity assay has been tested in solid tumours obtained from surgery. Various enzyme cocktails have been used for disaggregating cells for times ranging from 1–72 h. Collagenase (0.5%) plus DNase (0.002%) was chosen as producing the best results when incubated for 3 h at 37°C. This cocktail was also used for 24, 48 and 72 h incubations. Cell suspensions so produced were sometimes cleaned up by centrifugation on a Nycodenz gradient. Breast tumours were more often successfully cultured (67%) compared to gastrointestinal (43%) or other tumours (34%). Results with 6 drugs tested in 10 or more tumours suggested that in general, higher drug concentrations should be used when testing solid tumours as opposed to leukaemias in the DiSC assay. Springer Online Journal Archives 1860-2002 Bosanquet, Andrew G. oth Forskitt, Susan oth in Cytotechnology 1987 2(1989) vom: März, Seite 225-232 (DE-627)NLEJ188989684 (DE-600)1477657-1 1573-0778 nnns volume:2 year:1989 month:03 pages:225-232 extent:8 http://dx.doi.org/10.1007/BF00133247 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 2 1989 3 225-232 8 |
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(DE-627)NLEJ193656949 DE-627 ger DE-627 rakwb eng Effect of cell isolation methods and drug concentration on the use of the Differential Staining Cytotoxicity (DiSC) assay with solid tumours 1989 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract The Differential Staining Cytotoxicity (DiSC) in vitro drug sensitivity assay has been tested in solid tumours obtained from surgery. Various enzyme cocktails have been used for disaggregating cells for times ranging from 1–72 h. Collagenase (0.5%) plus DNase (0.002%) was chosen as producing the best results when incubated for 3 h at 37°C. This cocktail was also used for 24, 48 and 72 h incubations. Cell suspensions so produced were sometimes cleaned up by centrifugation on a Nycodenz gradient. Breast tumours were more often successfully cultured (67%) compared to gastrointestinal (43%) or other tumours (34%). Results with 6 drugs tested in 10 or more tumours suggested that in general, higher drug concentrations should be used when testing solid tumours as opposed to leukaemias in the DiSC assay. Springer Online Journal Archives 1860-2002 Bosanquet, Andrew G. oth Forskitt, Susan oth in Cytotechnology 1987 2(1989) vom: März, Seite 225-232 (DE-627)NLEJ188989684 (DE-600)1477657-1 1573-0778 nnns volume:2 year:1989 month:03 pages:225-232 extent:8 http://dx.doi.org/10.1007/BF00133247 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 2 1989 3 225-232 8 |
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(DE-627)NLEJ193656949 DE-627 ger DE-627 rakwb eng Effect of cell isolation methods and drug concentration on the use of the Differential Staining Cytotoxicity (DiSC) assay with solid tumours 1989 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract The Differential Staining Cytotoxicity (DiSC) in vitro drug sensitivity assay has been tested in solid tumours obtained from surgery. Various enzyme cocktails have been used for disaggregating cells for times ranging from 1–72 h. Collagenase (0.5%) plus DNase (0.002%) was chosen as producing the best results when incubated for 3 h at 37°C. This cocktail was also used for 24, 48 and 72 h incubations. Cell suspensions so produced were sometimes cleaned up by centrifugation on a Nycodenz gradient. Breast tumours were more often successfully cultured (67%) compared to gastrointestinal (43%) or other tumours (34%). Results with 6 drugs tested in 10 or more tumours suggested that in general, higher drug concentrations should be used when testing solid tumours as opposed to leukaemias in the DiSC assay. Springer Online Journal Archives 1860-2002 Bosanquet, Andrew G. oth Forskitt, Susan oth in Cytotechnology 1987 2(1989) vom: März, Seite 225-232 (DE-627)NLEJ188989684 (DE-600)1477657-1 1573-0778 nnns volume:2 year:1989 month:03 pages:225-232 extent:8 http://dx.doi.org/10.1007/BF00133247 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 2 1989 3 225-232 8 |
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(DE-627)NLEJ193656949 DE-627 ger DE-627 rakwb eng Effect of cell isolation methods and drug concentration on the use of the Differential Staining Cytotoxicity (DiSC) assay with solid tumours 1989 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract The Differential Staining Cytotoxicity (DiSC) in vitro drug sensitivity assay has been tested in solid tumours obtained from surgery. Various enzyme cocktails have been used for disaggregating cells for times ranging from 1–72 h. Collagenase (0.5%) plus DNase (0.002%) was chosen as producing the best results when incubated for 3 h at 37°C. This cocktail was also used for 24, 48 and 72 h incubations. Cell suspensions so produced were sometimes cleaned up by centrifugation on a Nycodenz gradient. Breast tumours were more often successfully cultured (67%) compared to gastrointestinal (43%) or other tumours (34%). Results with 6 drugs tested in 10 or more tumours suggested that in general, higher drug concentrations should be used when testing solid tumours as opposed to leukaemias in the DiSC assay. Springer Online Journal Archives 1860-2002 Bosanquet, Andrew G. oth Forskitt, Susan oth in Cytotechnology 1987 2(1989) vom: März, Seite 225-232 (DE-627)NLEJ188989684 (DE-600)1477657-1 1573-0778 nnns volume:2 year:1989 month:03 pages:225-232 extent:8 http://dx.doi.org/10.1007/BF00133247 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 2 1989 3 225-232 8 |
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(DE-627)NLEJ193656949 DE-627 ger DE-627 rakwb eng Effect of cell isolation methods and drug concentration on the use of the Differential Staining Cytotoxicity (DiSC) assay with solid tumours 1989 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract The Differential Staining Cytotoxicity (DiSC) in vitro drug sensitivity assay has been tested in solid tumours obtained from surgery. Various enzyme cocktails have been used for disaggregating cells for times ranging from 1–72 h. Collagenase (0.5%) plus DNase (0.002%) was chosen as producing the best results when incubated for 3 h at 37°C. This cocktail was also used for 24, 48 and 72 h incubations. Cell suspensions so produced were sometimes cleaned up by centrifugation on a Nycodenz gradient. Breast tumours were more often successfully cultured (67%) compared to gastrointestinal (43%) or other tumours (34%). Results with 6 drugs tested in 10 or more tumours suggested that in general, higher drug concentrations should be used when testing solid tumours as opposed to leukaemias in the DiSC assay. Springer Online Journal Archives 1860-2002 Bosanquet, Andrew G. oth Forskitt, Susan oth in Cytotechnology 1987 2(1989) vom: März, Seite 225-232 (DE-627)NLEJ188989684 (DE-600)1477657-1 1573-0778 nnns volume:2 year:1989 month:03 pages:225-232 extent:8 http://dx.doi.org/10.1007/BF00133247 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 2 1989 3 225-232 8 |
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effect of cell isolation methods and drug concentration on the use of the differential staining cytotoxicity (disc) assay with solid tumours |
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Effect of cell isolation methods and drug concentration on the use of the Differential Staining Cytotoxicity (DiSC) assay with solid tumours |
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Abstract The Differential Staining Cytotoxicity (DiSC) in vitro drug sensitivity assay has been tested in solid tumours obtained from surgery. Various enzyme cocktails have been used for disaggregating cells for times ranging from 1–72 h. Collagenase (0.5%) plus DNase (0.002%) was chosen as producing the best results when incubated for 3 h at 37°C. This cocktail was also used for 24, 48 and 72 h incubations. Cell suspensions so produced were sometimes cleaned up by centrifugation on a Nycodenz gradient. Breast tumours were more often successfully cultured (67%) compared to gastrointestinal (43%) or other tumours (34%). Results with 6 drugs tested in 10 or more tumours suggested that in general, higher drug concentrations should be used when testing solid tumours as opposed to leukaemias in the DiSC assay. |
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Abstract The Differential Staining Cytotoxicity (DiSC) in vitro drug sensitivity assay has been tested in solid tumours obtained from surgery. Various enzyme cocktails have been used for disaggregating cells for times ranging from 1–72 h. Collagenase (0.5%) plus DNase (0.002%) was chosen as producing the best results when incubated for 3 h at 37°C. This cocktail was also used for 24, 48 and 72 h incubations. Cell suspensions so produced were sometimes cleaned up by centrifugation on a Nycodenz gradient. Breast tumours were more often successfully cultured (67%) compared to gastrointestinal (43%) or other tumours (34%). Results with 6 drugs tested in 10 or more tumours suggested that in general, higher drug concentrations should be used when testing solid tumours as opposed to leukaemias in the DiSC assay. |
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Abstract The Differential Staining Cytotoxicity (DiSC) in vitro drug sensitivity assay has been tested in solid tumours obtained from surgery. Various enzyme cocktails have been used for disaggregating cells for times ranging from 1–72 h. Collagenase (0.5%) plus DNase (0.002%) was chosen as producing the best results when incubated for 3 h at 37°C. This cocktail was also used for 24, 48 and 72 h incubations. Cell suspensions so produced were sometimes cleaned up by centrifugation on a Nycodenz gradient. Breast tumours were more often successfully cultured (67%) compared to gastrointestinal (43%) or other tumours (34%). Results with 6 drugs tested in 10 or more tumours suggested that in general, higher drug concentrations should be used when testing solid tumours as opposed to leukaemias in the DiSC assay. |
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