Detection and Discrimination of Wheat Spindle Streak Mosaic Virus and Wheat Yellow Mosaic Virus Using Multiplex RT-PCR
Abstract Wheat spindle streak mosaic virus (WSSMV) and wheat yellow mosaic virus (WYMV) are two closely related bymoviruses which cause significant yield losses in wheat. There is no molecular diagnostic protocol available for either virus nor are serological methods able to discriminate them. A mul...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
|---|
| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
1999 |
|---|
| Umfang: |
6 |
|---|
| Reproduktion: |
Springer Online Journal Archives 1860-2002 |
|---|---|
| Übergeordnetes Werk: |
in: European journal of plant pathology - 1937, 105(1999) vom: Sept., Seite 891-896 |
| Übergeordnetes Werk: |
volume:105 ; year:1999 ; month:09 ; pages:891-896 ; extent:6 |
| Links: |
|---|
| Katalog-ID: |
NLEJ193742519 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLEJ193742519 | ||
| 003 | DE-627 | ||
| 005 | 20230506105254.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 070526s1999 xx |||||o 00| ||eng c | ||
| 035 | |a (DE-627)NLEJ193742519 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 245 | 1 | 0 | |a Detection and Discrimination of Wheat Spindle Streak Mosaic Virus and Wheat Yellow Mosaic Virus Using Multiplex RT-PCR |
| 264 | 1 | |c 1999 | |
| 300 | |a 6 | ||
| 336 | |a nicht spezifiziert |b zzz |2 rdacontent | ||
| 337 | |a nicht spezifiziert |b z |2 rdamedia | ||
| 338 | |a nicht spezifiziert |b zu |2 rdacarrier | ||
| 520 | |a Abstract Wheat spindle streak mosaic virus (WSSMV) and wheat yellow mosaic virus (WYMV) are two closely related bymoviruses which cause significant yield losses in wheat. There is no molecular diagnostic protocol available for either virus nor are serological methods able to discriminate them. A multiplex reverse transcription-polymerase chain reaction (RT-PCR) protocol was developed for their detection and discrimination. Twenty-three isolates of WSSMV and WYMV were collected from a range of countries and the sequence of 834–837 nucleotides of the coat protein gene of three representative WSSMV isolates and one WYMV isolate was determined. This sequence data provided further evidence that the two viruses are distinct species and was used together with previously published sequence data to design specific oligonucleotide primers to discriminate the two viruses. Using total RNA isolated from eighteen WSSMV and five WYMV isolates in either one-step or two-step RT-PCR, these primers were shown to detect and discriminate the two viruses reliably. The primers were specific to either WSSMV or WYMV and no PCR product was obtained with either soil-borne wheat mosaic virus, which is frequently associated with both diseases, or with the closely related viruses, barley yellow mosaic virus, barley mild mosaic virus or oat mosaic virus. This new diagnostic protocol will enable more effective management of the diseases caused by these viruses by enabling correct identification of the causal pathogen and earlier detection than is possible by serological methods. | ||
| 533 | |f Springer Online Journal Archives 1860-2002 | ||
| 700 | 1 | |a Clover, Gerard |4 oth | |
| 700 | 1 | |a Henry, Christine |4 oth | |
| 773 | 0 | 8 | |i in |t European journal of plant pathology |d 1937 |g 105(1999) vom: Sept., Seite 891-896 |w (DE-627)NLEJ188990887 |w (DE-600)1477679-0 |x 1573-8469 |7 nnns |
| 773 | 1 | 8 | |g volume:105 |g year:1999 |g month:09 |g pages:891-896 |g extent:6 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1023/A:1008707331487 |
| 912 | |a GBV_USEFLAG_U | ||
| 912 | |a ZDB-1-SOJ | ||
| 912 | |a GBV_NL_ARTICLE | ||
| 951 | |a AR | ||
| 952 | |d 105 |j 1999 |c 9 |h 891-896 |g 6 | ||
| matchkey_str |
article:15738469:1999----::eetoadiciiainfhasidetekoaciuadhayloms |
|---|---|
| hierarchy_sort_str |
1999 |
| publishDate |
1999 |
| allfields |
(DE-627)NLEJ193742519 DE-627 ger DE-627 rakwb eng Detection and Discrimination of Wheat Spindle Streak Mosaic Virus and Wheat Yellow Mosaic Virus Using Multiplex RT-PCR 1999 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract Wheat spindle streak mosaic virus (WSSMV) and wheat yellow mosaic virus (WYMV) are two closely related bymoviruses which cause significant yield losses in wheat. There is no molecular diagnostic protocol available for either virus nor are serological methods able to discriminate them. A multiplex reverse transcription-polymerase chain reaction (RT-PCR) protocol was developed for their detection and discrimination. Twenty-three isolates of WSSMV and WYMV were collected from a range of countries and the sequence of 834–837 nucleotides of the coat protein gene of three representative WSSMV isolates and one WYMV isolate was determined. This sequence data provided further evidence that the two viruses are distinct species and was used together with previously published sequence data to design specific oligonucleotide primers to discriminate the two viruses. Using total RNA isolated from eighteen WSSMV and five WYMV isolates in either one-step or two-step RT-PCR, these primers were shown to detect and discriminate the two viruses reliably. The primers were specific to either WSSMV or WYMV and no PCR product was obtained with either soil-borne wheat mosaic virus, which is frequently associated with both diseases, or with the closely related viruses, barley yellow mosaic virus, barley mild mosaic virus or oat mosaic virus. This new diagnostic protocol will enable more effective management of the diseases caused by these viruses by enabling correct identification of the causal pathogen and earlier detection than is possible by serological methods. Springer Online Journal Archives 1860-2002 Clover, Gerard oth Henry, Christine oth in European journal of plant pathology 1937 105(1999) vom: Sept., Seite 891-896 (DE-627)NLEJ188990887 (DE-600)1477679-0 1573-8469 nnns volume:105 year:1999 month:09 pages:891-896 extent:6 http://dx.doi.org/10.1023/A:1008707331487 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 105 1999 9 891-896 6 |
| spelling |
(DE-627)NLEJ193742519 DE-627 ger DE-627 rakwb eng Detection and Discrimination of Wheat Spindle Streak Mosaic Virus and Wheat Yellow Mosaic Virus Using Multiplex RT-PCR 1999 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract Wheat spindle streak mosaic virus (WSSMV) and wheat yellow mosaic virus (WYMV) are two closely related bymoviruses which cause significant yield losses in wheat. There is no molecular diagnostic protocol available for either virus nor are serological methods able to discriminate them. A multiplex reverse transcription-polymerase chain reaction (RT-PCR) protocol was developed for their detection and discrimination. Twenty-three isolates of WSSMV and WYMV were collected from a range of countries and the sequence of 834–837 nucleotides of the coat protein gene of three representative WSSMV isolates and one WYMV isolate was determined. This sequence data provided further evidence that the two viruses are distinct species and was used together with previously published sequence data to design specific oligonucleotide primers to discriminate the two viruses. Using total RNA isolated from eighteen WSSMV and five WYMV isolates in either one-step or two-step RT-PCR, these primers were shown to detect and discriminate the two viruses reliably. The primers were specific to either WSSMV or WYMV and no PCR product was obtained with either soil-borne wheat mosaic virus, which is frequently associated with both diseases, or with the closely related viruses, barley yellow mosaic virus, barley mild mosaic virus or oat mosaic virus. This new diagnostic protocol will enable more effective management of the diseases caused by these viruses by enabling correct identification of the causal pathogen and earlier detection than is possible by serological methods. Springer Online Journal Archives 1860-2002 Clover, Gerard oth Henry, Christine oth in European journal of plant pathology 1937 105(1999) vom: Sept., Seite 891-896 (DE-627)NLEJ188990887 (DE-600)1477679-0 1573-8469 nnns volume:105 year:1999 month:09 pages:891-896 extent:6 http://dx.doi.org/10.1023/A:1008707331487 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 105 1999 9 891-896 6 |
| allfields_unstemmed |
(DE-627)NLEJ193742519 DE-627 ger DE-627 rakwb eng Detection and Discrimination of Wheat Spindle Streak Mosaic Virus and Wheat Yellow Mosaic Virus Using Multiplex RT-PCR 1999 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract Wheat spindle streak mosaic virus (WSSMV) and wheat yellow mosaic virus (WYMV) are two closely related bymoviruses which cause significant yield losses in wheat. There is no molecular diagnostic protocol available for either virus nor are serological methods able to discriminate them. A multiplex reverse transcription-polymerase chain reaction (RT-PCR) protocol was developed for their detection and discrimination. Twenty-three isolates of WSSMV and WYMV were collected from a range of countries and the sequence of 834–837 nucleotides of the coat protein gene of three representative WSSMV isolates and one WYMV isolate was determined. This sequence data provided further evidence that the two viruses are distinct species and was used together with previously published sequence data to design specific oligonucleotide primers to discriminate the two viruses. Using total RNA isolated from eighteen WSSMV and five WYMV isolates in either one-step or two-step RT-PCR, these primers were shown to detect and discriminate the two viruses reliably. The primers were specific to either WSSMV or WYMV and no PCR product was obtained with either soil-borne wheat mosaic virus, which is frequently associated with both diseases, or with the closely related viruses, barley yellow mosaic virus, barley mild mosaic virus or oat mosaic virus. This new diagnostic protocol will enable more effective management of the diseases caused by these viruses by enabling correct identification of the causal pathogen and earlier detection than is possible by serological methods. Springer Online Journal Archives 1860-2002 Clover, Gerard oth Henry, Christine oth in European journal of plant pathology 1937 105(1999) vom: Sept., Seite 891-896 (DE-627)NLEJ188990887 (DE-600)1477679-0 1573-8469 nnns volume:105 year:1999 month:09 pages:891-896 extent:6 http://dx.doi.org/10.1023/A:1008707331487 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 105 1999 9 891-896 6 |
| allfieldsGer |
(DE-627)NLEJ193742519 DE-627 ger DE-627 rakwb eng Detection and Discrimination of Wheat Spindle Streak Mosaic Virus and Wheat Yellow Mosaic Virus Using Multiplex RT-PCR 1999 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract Wheat spindle streak mosaic virus (WSSMV) and wheat yellow mosaic virus (WYMV) are two closely related bymoviruses which cause significant yield losses in wheat. There is no molecular diagnostic protocol available for either virus nor are serological methods able to discriminate them. A multiplex reverse transcription-polymerase chain reaction (RT-PCR) protocol was developed for their detection and discrimination. Twenty-three isolates of WSSMV and WYMV were collected from a range of countries and the sequence of 834–837 nucleotides of the coat protein gene of three representative WSSMV isolates and one WYMV isolate was determined. This sequence data provided further evidence that the two viruses are distinct species and was used together with previously published sequence data to design specific oligonucleotide primers to discriminate the two viruses. Using total RNA isolated from eighteen WSSMV and five WYMV isolates in either one-step or two-step RT-PCR, these primers were shown to detect and discriminate the two viruses reliably. The primers were specific to either WSSMV or WYMV and no PCR product was obtained with either soil-borne wheat mosaic virus, which is frequently associated with both diseases, or with the closely related viruses, barley yellow mosaic virus, barley mild mosaic virus or oat mosaic virus. This new diagnostic protocol will enable more effective management of the diseases caused by these viruses by enabling correct identification of the causal pathogen and earlier detection than is possible by serological methods. Springer Online Journal Archives 1860-2002 Clover, Gerard oth Henry, Christine oth in European journal of plant pathology 1937 105(1999) vom: Sept., Seite 891-896 (DE-627)NLEJ188990887 (DE-600)1477679-0 1573-8469 nnns volume:105 year:1999 month:09 pages:891-896 extent:6 http://dx.doi.org/10.1023/A:1008707331487 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 105 1999 9 891-896 6 |
| allfieldsSound |
(DE-627)NLEJ193742519 DE-627 ger DE-627 rakwb eng Detection and Discrimination of Wheat Spindle Streak Mosaic Virus and Wheat Yellow Mosaic Virus Using Multiplex RT-PCR 1999 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract Wheat spindle streak mosaic virus (WSSMV) and wheat yellow mosaic virus (WYMV) are two closely related bymoviruses which cause significant yield losses in wheat. There is no molecular diagnostic protocol available for either virus nor are serological methods able to discriminate them. A multiplex reverse transcription-polymerase chain reaction (RT-PCR) protocol was developed for their detection and discrimination. Twenty-three isolates of WSSMV and WYMV were collected from a range of countries and the sequence of 834–837 nucleotides of the coat protein gene of three representative WSSMV isolates and one WYMV isolate was determined. This sequence data provided further evidence that the two viruses are distinct species and was used together with previously published sequence data to design specific oligonucleotide primers to discriminate the two viruses. Using total RNA isolated from eighteen WSSMV and five WYMV isolates in either one-step or two-step RT-PCR, these primers were shown to detect and discriminate the two viruses reliably. The primers were specific to either WSSMV or WYMV and no PCR product was obtained with either soil-borne wheat mosaic virus, which is frequently associated with both diseases, or with the closely related viruses, barley yellow mosaic virus, barley mild mosaic virus or oat mosaic virus. This new diagnostic protocol will enable more effective management of the diseases caused by these viruses by enabling correct identification of the causal pathogen and earlier detection than is possible by serological methods. Springer Online Journal Archives 1860-2002 Clover, Gerard oth Henry, Christine oth in European journal of plant pathology 1937 105(1999) vom: Sept., Seite 891-896 (DE-627)NLEJ188990887 (DE-600)1477679-0 1573-8469 nnns volume:105 year:1999 month:09 pages:891-896 extent:6 http://dx.doi.org/10.1023/A:1008707331487 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 105 1999 9 891-896 6 |
| language |
English |
| source |
in European journal of plant pathology 105(1999) vom: Sept., Seite 891-896 volume:105 year:1999 month:09 pages:891-896 extent:6 |
| sourceStr |
in European journal of plant pathology 105(1999) vom: Sept., Seite 891-896 volume:105 year:1999 month:09 pages:891-896 extent:6 |
| format_phy_str_mv |
Article |
| institution |
findex.gbv.de |
| isfreeaccess_bool |
false |
| container_title |
European journal of plant pathology |
| authorswithroles_txt_mv |
Clover, Gerard @@oth@@ Henry, Christine @@oth@@ |
| publishDateDaySort_date |
1999-09-01T00:00:00Z |
| hierarchy_top_id |
NLEJ188990887 |
| id |
NLEJ193742519 |
| language_de |
englisch |
| fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ193742519</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230506105254.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070526s1999 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ193742519</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Detection and Discrimination of Wheat Spindle Streak Mosaic Virus and Wheat Yellow Mosaic Virus Using Multiplex RT-PCR</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1999</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">6</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Wheat spindle streak mosaic virus (WSSMV) and wheat yellow mosaic virus (WYMV) are two closely related bymoviruses which cause significant yield losses in wheat. There is no molecular diagnostic protocol available for either virus nor are serological methods able to discriminate them. A multiplex reverse transcription-polymerase chain reaction (RT-PCR) protocol was developed for their detection and discrimination. Twenty-three isolates of WSSMV and WYMV were collected from a range of countries and the sequence of 834–837 nucleotides of the coat protein gene of three representative WSSMV isolates and one WYMV isolate was determined. This sequence data provided further evidence that the two viruses are distinct species and was used together with previously published sequence data to design specific oligonucleotide primers to discriminate the two viruses. Using total RNA isolated from eighteen WSSMV and five WYMV isolates in either one-step or two-step RT-PCR, these primers were shown to detect and discriminate the two viruses reliably. The primers were specific to either WSSMV or WYMV and no PCR product was obtained with either soil-borne wheat mosaic virus, which is frequently associated with both diseases, or with the closely related viruses, barley yellow mosaic virus, barley mild mosaic virus or oat mosaic virus. This new diagnostic protocol will enable more effective management of the diseases caused by these viruses by enabling correct identification of the causal pathogen and earlier detection than is possible by serological methods.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Springer Online Journal Archives 1860-2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Clover, Gerard</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Henry, Christine</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">European journal of plant pathology</subfield><subfield code="d">1937</subfield><subfield code="g">105(1999) vom: Sept., Seite 891-896</subfield><subfield code="w">(DE-627)NLEJ188990887</subfield><subfield code="w">(DE-600)1477679-0</subfield><subfield code="x">1573-8469</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:105</subfield><subfield code="g">year:1999</subfield><subfield code="g">month:09</subfield><subfield code="g">pages:891-896</subfield><subfield code="g">extent:6</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1023/A:1008707331487</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SOJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">105</subfield><subfield code="j">1999</subfield><subfield code="c">9</subfield><subfield code="h">891-896</subfield><subfield code="g">6</subfield></datafield></record></collection>
|
| series2 |
Springer Online Journal Archives 1860-2002 |
| ppnlink_with_tag_str_mv |
@@773@@(DE-627)NLEJ188990887 |
| format |
electronic Article |
| delete_txt_mv |
keep |
| collection |
NL |
| remote_str |
true |
| illustrated |
Not Illustrated |
| issn |
1573-8469 |
| topic_title |
Detection and Discrimination of Wheat Spindle Streak Mosaic Virus and Wheat Yellow Mosaic Virus Using Multiplex RT-PCR |
| format_facet |
Elektronische Aufsätze Aufsätze Elektronische Ressource |
| format_main_str_mv |
Text Zeitschrift/Artikel |
| carriertype_str_mv |
zu |
| author2_variant |
g c gc c h ch |
| hierarchy_parent_title |
European journal of plant pathology |
| hierarchy_parent_id |
NLEJ188990887 |
| hierarchy_top_title |
European journal of plant pathology |
| isfreeaccess_txt |
false |
| familylinks_str_mv |
(DE-627)NLEJ188990887 (DE-600)1477679-0 |
| title |
Detection and Discrimination of Wheat Spindle Streak Mosaic Virus and Wheat Yellow Mosaic Virus Using Multiplex RT-PCR |
| spellingShingle |
Detection and Discrimination of Wheat Spindle Streak Mosaic Virus and Wheat Yellow Mosaic Virus Using Multiplex RT-PCR |
| ctrlnum |
(DE-627)NLEJ193742519 |
| title_full |
Detection and Discrimination of Wheat Spindle Streak Mosaic Virus and Wheat Yellow Mosaic Virus Using Multiplex RT-PCR |
| journal |
European journal of plant pathology |
| journalStr |
European journal of plant pathology |
| lang_code |
eng |
| isOA_bool |
false |
| recordtype |
marc |
| publishDateSort |
1999 |
| contenttype_str_mv |
zzz |
| container_start_page |
891 |
| container_volume |
105 |
| physical |
6 |
| format_se |
Elektronische Aufsätze |
| title_sort |
detection and discrimination of wheat spindle streak mosaic virus and wheat yellow mosaic virus using multiplex rt-pcr |
| title_auth |
Detection and Discrimination of Wheat Spindle Streak Mosaic Virus and Wheat Yellow Mosaic Virus Using Multiplex RT-PCR |
| abstract |
Abstract Wheat spindle streak mosaic virus (WSSMV) and wheat yellow mosaic virus (WYMV) are two closely related bymoviruses which cause significant yield losses in wheat. There is no molecular diagnostic protocol available for either virus nor are serological methods able to discriminate them. A multiplex reverse transcription-polymerase chain reaction (RT-PCR) protocol was developed for their detection and discrimination. Twenty-three isolates of WSSMV and WYMV were collected from a range of countries and the sequence of 834–837 nucleotides of the coat protein gene of three representative WSSMV isolates and one WYMV isolate was determined. This sequence data provided further evidence that the two viruses are distinct species and was used together with previously published sequence data to design specific oligonucleotide primers to discriminate the two viruses. Using total RNA isolated from eighteen WSSMV and five WYMV isolates in either one-step or two-step RT-PCR, these primers were shown to detect and discriminate the two viruses reliably. The primers were specific to either WSSMV or WYMV and no PCR product was obtained with either soil-borne wheat mosaic virus, which is frequently associated with both diseases, or with the closely related viruses, barley yellow mosaic virus, barley mild mosaic virus or oat mosaic virus. This new diagnostic protocol will enable more effective management of the diseases caused by these viruses by enabling correct identification of the causal pathogen and earlier detection than is possible by serological methods. |
| abstractGer |
Abstract Wheat spindle streak mosaic virus (WSSMV) and wheat yellow mosaic virus (WYMV) are two closely related bymoviruses which cause significant yield losses in wheat. There is no molecular diagnostic protocol available for either virus nor are serological methods able to discriminate them. A multiplex reverse transcription-polymerase chain reaction (RT-PCR) protocol was developed for their detection and discrimination. Twenty-three isolates of WSSMV and WYMV were collected from a range of countries and the sequence of 834–837 nucleotides of the coat protein gene of three representative WSSMV isolates and one WYMV isolate was determined. This sequence data provided further evidence that the two viruses are distinct species and was used together with previously published sequence data to design specific oligonucleotide primers to discriminate the two viruses. Using total RNA isolated from eighteen WSSMV and five WYMV isolates in either one-step or two-step RT-PCR, these primers were shown to detect and discriminate the two viruses reliably. The primers were specific to either WSSMV or WYMV and no PCR product was obtained with either soil-borne wheat mosaic virus, which is frequently associated with both diseases, or with the closely related viruses, barley yellow mosaic virus, barley mild mosaic virus or oat mosaic virus. This new diagnostic protocol will enable more effective management of the diseases caused by these viruses by enabling correct identification of the causal pathogen and earlier detection than is possible by serological methods. |
| abstract_unstemmed |
Abstract Wheat spindle streak mosaic virus (WSSMV) and wheat yellow mosaic virus (WYMV) are two closely related bymoviruses which cause significant yield losses in wheat. There is no molecular diagnostic protocol available for either virus nor are serological methods able to discriminate them. A multiplex reverse transcription-polymerase chain reaction (RT-PCR) protocol was developed for their detection and discrimination. Twenty-three isolates of WSSMV and WYMV were collected from a range of countries and the sequence of 834–837 nucleotides of the coat protein gene of three representative WSSMV isolates and one WYMV isolate was determined. This sequence data provided further evidence that the two viruses are distinct species and was used together with previously published sequence data to design specific oligonucleotide primers to discriminate the two viruses. Using total RNA isolated from eighteen WSSMV and five WYMV isolates in either one-step or two-step RT-PCR, these primers were shown to detect and discriminate the two viruses reliably. The primers were specific to either WSSMV or WYMV and no PCR product was obtained with either soil-borne wheat mosaic virus, which is frequently associated with both diseases, or with the closely related viruses, barley yellow mosaic virus, barley mild mosaic virus or oat mosaic virus. This new diagnostic protocol will enable more effective management of the diseases caused by these viruses by enabling correct identification of the causal pathogen and earlier detection than is possible by serological methods. |
| collection_details |
GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE |
| title_short |
Detection and Discrimination of Wheat Spindle Streak Mosaic Virus and Wheat Yellow Mosaic Virus Using Multiplex RT-PCR |
| url |
http://dx.doi.org/10.1023/A:1008707331487 |
| remote_bool |
true |
| author2 |
Clover, Gerard Henry, Christine |
| author2Str |
Clover, Gerard Henry, Christine |
| ppnlink |
NLEJ188990887 |
| mediatype_str_mv |
z |
| isOA_txt |
false |
| hochschulschrift_bool |
false |
| author2_role |
oth oth |
| up_date |
2024-07-05T22:34:27.591Z |
| _version_ |
1803780219307294720 |
| fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ193742519</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230506105254.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070526s1999 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ193742519</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Detection and Discrimination of Wheat Spindle Streak Mosaic Virus and Wheat Yellow Mosaic Virus Using Multiplex RT-PCR</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1999</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">6</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Wheat spindle streak mosaic virus (WSSMV) and wheat yellow mosaic virus (WYMV) are two closely related bymoviruses which cause significant yield losses in wheat. There is no molecular diagnostic protocol available for either virus nor are serological methods able to discriminate them. A multiplex reverse transcription-polymerase chain reaction (RT-PCR) protocol was developed for their detection and discrimination. Twenty-three isolates of WSSMV and WYMV were collected from a range of countries and the sequence of 834–837 nucleotides of the coat protein gene of three representative WSSMV isolates and one WYMV isolate was determined. This sequence data provided further evidence that the two viruses are distinct species and was used together with previously published sequence data to design specific oligonucleotide primers to discriminate the two viruses. Using total RNA isolated from eighteen WSSMV and five WYMV isolates in either one-step or two-step RT-PCR, these primers were shown to detect and discriminate the two viruses reliably. The primers were specific to either WSSMV or WYMV and no PCR product was obtained with either soil-borne wheat mosaic virus, which is frequently associated with both diseases, or with the closely related viruses, barley yellow mosaic virus, barley mild mosaic virus or oat mosaic virus. This new diagnostic protocol will enable more effective management of the diseases caused by these viruses by enabling correct identification of the causal pathogen and earlier detection than is possible by serological methods.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Springer Online Journal Archives 1860-2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Clover, Gerard</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Henry, Christine</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">European journal of plant pathology</subfield><subfield code="d">1937</subfield><subfield code="g">105(1999) vom: Sept., Seite 891-896</subfield><subfield code="w">(DE-627)NLEJ188990887</subfield><subfield code="w">(DE-600)1477679-0</subfield><subfield code="x">1573-8469</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:105</subfield><subfield code="g">year:1999</subfield><subfield code="g">month:09</subfield><subfield code="g">pages:891-896</subfield><subfield code="g">extent:6</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1023/A:1008707331487</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SOJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">105</subfield><subfield code="j">1999</subfield><subfield code="c">9</subfield><subfield code="h">891-896</subfield><subfield code="g">6</subfield></datafield></record></collection>
|
| score |
7.400712 |