Secretion of the overproduced periplasmic PhoA protein into the medium and accumulation of its precursor in phoA-transformed Escherichia coli strains: involvement of outer membrane vesicles
Abstract Various Escherichia coli strains were transformed by multicopy plasmids pHI-1, pHI-7 and pPHOI carrying the entire regulatory and structural phoA sequences. All transformants with the intact pho regulatory system displayed PhoA oversynthesis and secretion into the medium. They also accumula...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1991 |
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| Umfang: |
13 |
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| Reproduktion: |
Springer Online Journal Archives 1860-2002 |
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| Übergeordnetes Werk: |
in: World journal of microbiology and biotechnology - 1985, 7(1991) vom: März, Seite 394-406 |
| Übergeordnetes Werk: |
volume:7 ; year:1991 ; month:03 ; pages:394-406 ; extent:13 |
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NLEJ196252849 |
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| 245 | 1 | 0 | |a Secretion of the overproduced periplasmic PhoA protein into the medium and accumulation of its precursor in phoA-transformed Escherichia coli strains: involvement of outer membrane vesicles |
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| 520 | |a Abstract Various Escherichia coli strains were transformed by multicopy plasmids pHI-1, pHI-7 and pPHOI carrying the entire regulatory and structural phoA sequences. All transformants with the intact pho regulatory system displayed PhoA oversynthesis and secretion into the medium. They also accumulated the alkaline phosphatase precursor localized in the outer membrane fraction. The dynamics of enzyme synthesis and secretion as well as cell cytomorphology during secretion were studied in strain E. coli K12 802 carrying pHI-7 plasmid. PhoA protein was shown to be selectively released into the medium in vesicles budding from outer membrane. | ||
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| 700 | 1 | |a Nesmeyanova, M. A. |4 oth | |
| 700 | 1 | |a Tsfasman, I. M. |4 oth | |
| 700 | 1 | |a Karamyshev, A. L. |4 oth | |
| 700 | 1 | |a Suzina, N. E. |4 oth | |
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(DE-627)NLEJ196252849 DE-627 ger DE-627 rakwb eng Secretion of the overproduced periplasmic PhoA protein into the medium and accumulation of its precursor in phoA-transformed Escherichia coli strains: involvement of outer membrane vesicles 1991 13 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract Various Escherichia coli strains were transformed by multicopy plasmids pHI-1, pHI-7 and pPHOI carrying the entire regulatory and structural phoA sequences. All transformants with the intact pho regulatory system displayed PhoA oversynthesis and secretion into the medium. They also accumulated the alkaline phosphatase precursor localized in the outer membrane fraction. The dynamics of enzyme synthesis and secretion as well as cell cytomorphology during secretion were studied in strain E. coli K12 802 carrying pHI-7 plasmid. PhoA protein was shown to be selectively released into the medium in vesicles budding from outer membrane. Springer Online Journal Archives 1860-2002 Nesmeyanova, M. A. oth Tsfasman, I. M. oth Karamyshev, A. L. oth Suzina, N. E. oth in World journal of microbiology and biotechnology 1985 7(1991) vom: März, Seite 394-406 (DE-627)NLEJ188986316 (DE-600)1499109-3 1573-0972 nnns volume:7 year:1991 month:03 pages:394-406 extent:13 http://dx.doi.org/10.1007/BF00329408 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 7 1991 3 394-406 13 |
| spelling |
(DE-627)NLEJ196252849 DE-627 ger DE-627 rakwb eng Secretion of the overproduced periplasmic PhoA protein into the medium and accumulation of its precursor in phoA-transformed Escherichia coli strains: involvement of outer membrane vesicles 1991 13 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract Various Escherichia coli strains were transformed by multicopy plasmids pHI-1, pHI-7 and pPHOI carrying the entire regulatory and structural phoA sequences. All transformants with the intact pho regulatory system displayed PhoA oversynthesis and secretion into the medium. They also accumulated the alkaline phosphatase precursor localized in the outer membrane fraction. The dynamics of enzyme synthesis and secretion as well as cell cytomorphology during secretion were studied in strain E. coli K12 802 carrying pHI-7 plasmid. PhoA protein was shown to be selectively released into the medium in vesicles budding from outer membrane. Springer Online Journal Archives 1860-2002 Nesmeyanova, M. A. oth Tsfasman, I. M. oth Karamyshev, A. L. oth Suzina, N. E. oth in World journal of microbiology and biotechnology 1985 7(1991) vom: März, Seite 394-406 (DE-627)NLEJ188986316 (DE-600)1499109-3 1573-0972 nnns volume:7 year:1991 month:03 pages:394-406 extent:13 http://dx.doi.org/10.1007/BF00329408 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 7 1991 3 394-406 13 |
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(DE-627)NLEJ196252849 DE-627 ger DE-627 rakwb eng Secretion of the overproduced periplasmic PhoA protein into the medium and accumulation of its precursor in phoA-transformed Escherichia coli strains: involvement of outer membrane vesicles 1991 13 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract Various Escherichia coli strains were transformed by multicopy plasmids pHI-1, pHI-7 and pPHOI carrying the entire regulatory and structural phoA sequences. All transformants with the intact pho regulatory system displayed PhoA oversynthesis and secretion into the medium. They also accumulated the alkaline phosphatase precursor localized in the outer membrane fraction. The dynamics of enzyme synthesis and secretion as well as cell cytomorphology during secretion were studied in strain E. coli K12 802 carrying pHI-7 plasmid. PhoA protein was shown to be selectively released into the medium in vesicles budding from outer membrane. Springer Online Journal Archives 1860-2002 Nesmeyanova, M. A. oth Tsfasman, I. M. oth Karamyshev, A. L. oth Suzina, N. E. oth in World journal of microbiology and biotechnology 1985 7(1991) vom: März, Seite 394-406 (DE-627)NLEJ188986316 (DE-600)1499109-3 1573-0972 nnns volume:7 year:1991 month:03 pages:394-406 extent:13 http://dx.doi.org/10.1007/BF00329408 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 7 1991 3 394-406 13 |
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(DE-627)NLEJ196252849 DE-627 ger DE-627 rakwb eng Secretion of the overproduced periplasmic PhoA protein into the medium and accumulation of its precursor in phoA-transformed Escherichia coli strains: involvement of outer membrane vesicles 1991 13 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract Various Escherichia coli strains were transformed by multicopy plasmids pHI-1, pHI-7 and pPHOI carrying the entire regulatory and structural phoA sequences. All transformants with the intact pho regulatory system displayed PhoA oversynthesis and secretion into the medium. They also accumulated the alkaline phosphatase precursor localized in the outer membrane fraction. The dynamics of enzyme synthesis and secretion as well as cell cytomorphology during secretion were studied in strain E. coli K12 802 carrying pHI-7 plasmid. PhoA protein was shown to be selectively released into the medium in vesicles budding from outer membrane. Springer Online Journal Archives 1860-2002 Nesmeyanova, M. A. oth Tsfasman, I. M. oth Karamyshev, A. L. oth Suzina, N. E. oth in World journal of microbiology and biotechnology 1985 7(1991) vom: März, Seite 394-406 (DE-627)NLEJ188986316 (DE-600)1499109-3 1573-0972 nnns volume:7 year:1991 month:03 pages:394-406 extent:13 http://dx.doi.org/10.1007/BF00329408 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 7 1991 3 394-406 13 |
| allfieldsSound |
(DE-627)NLEJ196252849 DE-627 ger DE-627 rakwb eng Secretion of the overproduced periplasmic PhoA protein into the medium and accumulation of its precursor in phoA-transformed Escherichia coli strains: involvement of outer membrane vesicles 1991 13 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract Various Escherichia coli strains were transformed by multicopy plasmids pHI-1, pHI-7 and pPHOI carrying the entire regulatory and structural phoA sequences. All transformants with the intact pho regulatory system displayed PhoA oversynthesis and secretion into the medium. They also accumulated the alkaline phosphatase precursor localized in the outer membrane fraction. The dynamics of enzyme synthesis and secretion as well as cell cytomorphology during secretion were studied in strain E. coli K12 802 carrying pHI-7 plasmid. PhoA protein was shown to be selectively released into the medium in vesicles budding from outer membrane. Springer Online Journal Archives 1860-2002 Nesmeyanova, M. A. oth Tsfasman, I. M. oth Karamyshev, A. L. oth Suzina, N. E. oth in World journal of microbiology and biotechnology 1985 7(1991) vom: März, Seite 394-406 (DE-627)NLEJ188986316 (DE-600)1499109-3 1573-0972 nnns volume:7 year:1991 month:03 pages:394-406 extent:13 http://dx.doi.org/10.1007/BF00329408 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 7 1991 3 394-406 13 |
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Secretion of the overproduced periplasmic PhoA protein into the medium and accumulation of its precursor in phoA-transformed Escherichia coli strains: involvement of outer membrane vesicles |
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Secretion of the overproduced periplasmic PhoA protein into the medium and accumulation of its precursor in phoA-transformed Escherichia coli strains: involvement of outer membrane vesicles |
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secretion of the overproduced periplasmic phoa protein into the medium and accumulation of its precursor in phoa-transformed escherichia coli strains: involvement of outer membrane vesicles |
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Secretion of the overproduced periplasmic PhoA protein into the medium and accumulation of its precursor in phoA-transformed Escherichia coli strains: involvement of outer membrane vesicles |
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Abstract Various Escherichia coli strains were transformed by multicopy plasmids pHI-1, pHI-7 and pPHOI carrying the entire regulatory and structural phoA sequences. All transformants with the intact pho regulatory system displayed PhoA oversynthesis and secretion into the medium. They also accumulated the alkaline phosphatase precursor localized in the outer membrane fraction. The dynamics of enzyme synthesis and secretion as well as cell cytomorphology during secretion were studied in strain E. coli K12 802 carrying pHI-7 plasmid. PhoA protein was shown to be selectively released into the medium in vesicles budding from outer membrane. |
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Abstract Various Escherichia coli strains were transformed by multicopy plasmids pHI-1, pHI-7 and pPHOI carrying the entire regulatory and structural phoA sequences. All transformants with the intact pho regulatory system displayed PhoA oversynthesis and secretion into the medium. They also accumulated the alkaline phosphatase precursor localized in the outer membrane fraction. The dynamics of enzyme synthesis and secretion as well as cell cytomorphology during secretion were studied in strain E. coli K12 802 carrying pHI-7 plasmid. PhoA protein was shown to be selectively released into the medium in vesicles budding from outer membrane. |
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Abstract Various Escherichia coli strains were transformed by multicopy plasmids pHI-1, pHI-7 and pPHOI carrying the entire regulatory and structural phoA sequences. All transformants with the intact pho regulatory system displayed PhoA oversynthesis and secretion into the medium. They also accumulated the alkaline phosphatase precursor localized in the outer membrane fraction. The dynamics of enzyme synthesis and secretion as well as cell cytomorphology during secretion were studied in strain E. coli K12 802 carrying pHI-7 plasmid. PhoA protein was shown to be selectively released into the medium in vesicles budding from outer membrane. |
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Secretion of the overproduced periplasmic PhoA protein into the medium and accumulation of its precursor in phoA-transformed Escherichia coli strains: involvement of outer membrane vesicles |
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