Kinetics of enzymatic lysis, formation and regeneration of protoplasts ofCandida (Torulopsis) apicola
Abstract The optimal conditions for protoplast formation ofCandida apicola were by using an enzyme fromArthrobacter sp. in combination with 2-mercaptoethanol. The kinetic data support the two-layered structure model of cell wall for this yeast but the structure of the cell wall depended on the age o...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
|---|
| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
1992 |
|---|
| Umfang: |
7 |
|---|
| Reproduktion: |
Springer Online Journal Archives 1860-2002 |
|---|---|
| Übergeordnetes Werk: |
in: World journal of microbiology and biotechnology - 1985, 8(1992) vom: Jan., Seite 14-20 |
| Übergeordnetes Werk: |
volume:8 ; year:1992 ; month:01 ; pages:14-20 ; extent:7 |
| Links: |
|---|
| Katalog-ID: |
NLEJ196253438 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLEJ196253438 | ||
| 003 | DE-627 | ||
| 005 | 20230506161536.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 070526s1992 xx |||||o 00| ||eng c | ||
| 035 | |a (DE-627)NLEJ196253438 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 245 | 1 | 0 | |a Kinetics of enzymatic lysis, formation and regeneration of protoplasts ofCandida (Torulopsis) apicola |
| 264 | 1 | |c 1992 | |
| 300 | |a 7 | ||
| 336 | |a nicht spezifiziert |b zzz |2 rdacontent | ||
| 337 | |a nicht spezifiziert |b z |2 rdamedia | ||
| 338 | |a nicht spezifiziert |b zu |2 rdacarrier | ||
| 520 | |a Abstract The optimal conditions for protoplast formation ofCandida apicola were by using an enzyme fromArthrobacter sp. in combination with 2-mercaptoethanol. The kinetic data support the two-layered structure model of cell wall for this yeast but the structure of the cell wall depended on the age of cells and culture conditions. To regenerate the protoplasts, the type of osmotic stabilizer was important: sorbitol gave 16 to 30% regeneration. Electron microscopy revealed the presence of vesicles in the sections of protoplasts and whole cells ofCandida apicola grown in production medium and producing glycolipids. In sections of whole cells, vesicle-like structures are located in the periplasmic space and in protoplasts they can either be attached to, or released from, the cell surface. These vesicles are thought to be involved in the transport of the surface-active glycolipids and in the protection of the cell against denaturing effects. | ||
| 533 | |f Springer Online Journal Archives 1860-2002 | ||
| 700 | 1 | |a Rilke, O. |4 oth | |
| 700 | 1 | |a Baum, A. |4 oth | |
| 700 | 1 | |a Weiss, J. |4 oth | |
| 700 | 1 | |a Hommel, R. |4 oth | |
| 700 | 1 | |a Kleber, H. -P. |4 oth | |
| 773 | 0 | 8 | |i in |t World journal of microbiology and biotechnology |d 1985 |g 8(1992) vom: Jan., Seite 14-20 |w (DE-627)NLEJ188986316 |w (DE-600)1499109-3 |x 1573-0972 |7 nnns |
| 773 | 1 | 8 | |g volume:8 |g year:1992 |g month:01 |g pages:14-20 |g extent:7 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1007/BF01200677 |
| 912 | |a GBV_USEFLAG_U | ||
| 912 | |a ZDB-1-SOJ | ||
| 912 | |a GBV_NL_ARTICLE | ||
| 951 | |a AR | ||
| 952 | |d 8 |j 1992 |c 1 |h 14-20 |g 7 | ||
| matchkey_str |
article:15730972:1992----::ieisfnyailssomtoadeeeainfrtpato |
|---|---|
| hierarchy_sort_str |
1992 |
| publishDate |
1992 |
| allfields |
(DE-627)NLEJ196253438 DE-627 ger DE-627 rakwb eng Kinetics of enzymatic lysis, formation and regeneration of protoplasts ofCandida (Torulopsis) apicola 1992 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract The optimal conditions for protoplast formation ofCandida apicola were by using an enzyme fromArthrobacter sp. in combination with 2-mercaptoethanol. The kinetic data support the two-layered structure model of cell wall for this yeast but the structure of the cell wall depended on the age of cells and culture conditions. To regenerate the protoplasts, the type of osmotic stabilizer was important: sorbitol gave 16 to 30% regeneration. Electron microscopy revealed the presence of vesicles in the sections of protoplasts and whole cells ofCandida apicola grown in production medium and producing glycolipids. In sections of whole cells, vesicle-like structures are located in the periplasmic space and in protoplasts they can either be attached to, or released from, the cell surface. These vesicles are thought to be involved in the transport of the surface-active glycolipids and in the protection of the cell against denaturing effects. Springer Online Journal Archives 1860-2002 Rilke, O. oth Baum, A. oth Weiss, J. oth Hommel, R. oth Kleber, H. -P. oth in World journal of microbiology and biotechnology 1985 8(1992) vom: Jan., Seite 14-20 (DE-627)NLEJ188986316 (DE-600)1499109-3 1573-0972 nnns volume:8 year:1992 month:01 pages:14-20 extent:7 http://dx.doi.org/10.1007/BF01200677 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 8 1992 1 14-20 7 |
| spelling |
(DE-627)NLEJ196253438 DE-627 ger DE-627 rakwb eng Kinetics of enzymatic lysis, formation and regeneration of protoplasts ofCandida (Torulopsis) apicola 1992 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract The optimal conditions for protoplast formation ofCandida apicola were by using an enzyme fromArthrobacter sp. in combination with 2-mercaptoethanol. The kinetic data support the two-layered structure model of cell wall for this yeast but the structure of the cell wall depended on the age of cells and culture conditions. To regenerate the protoplasts, the type of osmotic stabilizer was important: sorbitol gave 16 to 30% regeneration. Electron microscopy revealed the presence of vesicles in the sections of protoplasts and whole cells ofCandida apicola grown in production medium and producing glycolipids. In sections of whole cells, vesicle-like structures are located in the periplasmic space and in protoplasts they can either be attached to, or released from, the cell surface. These vesicles are thought to be involved in the transport of the surface-active glycolipids and in the protection of the cell against denaturing effects. Springer Online Journal Archives 1860-2002 Rilke, O. oth Baum, A. oth Weiss, J. oth Hommel, R. oth Kleber, H. -P. oth in World journal of microbiology and biotechnology 1985 8(1992) vom: Jan., Seite 14-20 (DE-627)NLEJ188986316 (DE-600)1499109-3 1573-0972 nnns volume:8 year:1992 month:01 pages:14-20 extent:7 http://dx.doi.org/10.1007/BF01200677 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 8 1992 1 14-20 7 |
| allfields_unstemmed |
(DE-627)NLEJ196253438 DE-627 ger DE-627 rakwb eng Kinetics of enzymatic lysis, formation and regeneration of protoplasts ofCandida (Torulopsis) apicola 1992 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract The optimal conditions for protoplast formation ofCandida apicola were by using an enzyme fromArthrobacter sp. in combination with 2-mercaptoethanol. The kinetic data support the two-layered structure model of cell wall for this yeast but the structure of the cell wall depended on the age of cells and culture conditions. To regenerate the protoplasts, the type of osmotic stabilizer was important: sorbitol gave 16 to 30% regeneration. Electron microscopy revealed the presence of vesicles in the sections of protoplasts and whole cells ofCandida apicola grown in production medium and producing glycolipids. In sections of whole cells, vesicle-like structures are located in the periplasmic space and in protoplasts they can either be attached to, or released from, the cell surface. These vesicles are thought to be involved in the transport of the surface-active glycolipids and in the protection of the cell against denaturing effects. Springer Online Journal Archives 1860-2002 Rilke, O. oth Baum, A. oth Weiss, J. oth Hommel, R. oth Kleber, H. -P. oth in World journal of microbiology and biotechnology 1985 8(1992) vom: Jan., Seite 14-20 (DE-627)NLEJ188986316 (DE-600)1499109-3 1573-0972 nnns volume:8 year:1992 month:01 pages:14-20 extent:7 http://dx.doi.org/10.1007/BF01200677 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 8 1992 1 14-20 7 |
| allfieldsGer |
(DE-627)NLEJ196253438 DE-627 ger DE-627 rakwb eng Kinetics of enzymatic lysis, formation and regeneration of protoplasts ofCandida (Torulopsis) apicola 1992 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract The optimal conditions for protoplast formation ofCandida apicola were by using an enzyme fromArthrobacter sp. in combination with 2-mercaptoethanol. The kinetic data support the two-layered structure model of cell wall for this yeast but the structure of the cell wall depended on the age of cells and culture conditions. To regenerate the protoplasts, the type of osmotic stabilizer was important: sorbitol gave 16 to 30% regeneration. Electron microscopy revealed the presence of vesicles in the sections of protoplasts and whole cells ofCandida apicola grown in production medium and producing glycolipids. In sections of whole cells, vesicle-like structures are located in the periplasmic space and in protoplasts they can either be attached to, or released from, the cell surface. These vesicles are thought to be involved in the transport of the surface-active glycolipids and in the protection of the cell against denaturing effects. Springer Online Journal Archives 1860-2002 Rilke, O. oth Baum, A. oth Weiss, J. oth Hommel, R. oth Kleber, H. -P. oth in World journal of microbiology and biotechnology 1985 8(1992) vom: Jan., Seite 14-20 (DE-627)NLEJ188986316 (DE-600)1499109-3 1573-0972 nnns volume:8 year:1992 month:01 pages:14-20 extent:7 http://dx.doi.org/10.1007/BF01200677 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 8 1992 1 14-20 7 |
| allfieldsSound |
(DE-627)NLEJ196253438 DE-627 ger DE-627 rakwb eng Kinetics of enzymatic lysis, formation and regeneration of protoplasts ofCandida (Torulopsis) apicola 1992 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract The optimal conditions for protoplast formation ofCandida apicola were by using an enzyme fromArthrobacter sp. in combination with 2-mercaptoethanol. The kinetic data support the two-layered structure model of cell wall for this yeast but the structure of the cell wall depended on the age of cells and culture conditions. To regenerate the protoplasts, the type of osmotic stabilizer was important: sorbitol gave 16 to 30% regeneration. Electron microscopy revealed the presence of vesicles in the sections of protoplasts and whole cells ofCandida apicola grown in production medium and producing glycolipids. In sections of whole cells, vesicle-like structures are located in the periplasmic space and in protoplasts they can either be attached to, or released from, the cell surface. These vesicles are thought to be involved in the transport of the surface-active glycolipids and in the protection of the cell against denaturing effects. Springer Online Journal Archives 1860-2002 Rilke, O. oth Baum, A. oth Weiss, J. oth Hommel, R. oth Kleber, H. -P. oth in World journal of microbiology and biotechnology 1985 8(1992) vom: Jan., Seite 14-20 (DE-627)NLEJ188986316 (DE-600)1499109-3 1573-0972 nnns volume:8 year:1992 month:01 pages:14-20 extent:7 http://dx.doi.org/10.1007/BF01200677 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 8 1992 1 14-20 7 |
| language |
English |
| source |
in World journal of microbiology and biotechnology 8(1992) vom: Jan., Seite 14-20 volume:8 year:1992 month:01 pages:14-20 extent:7 |
| sourceStr |
in World journal of microbiology and biotechnology 8(1992) vom: Jan., Seite 14-20 volume:8 year:1992 month:01 pages:14-20 extent:7 |
| format_phy_str_mv |
Article |
| institution |
findex.gbv.de |
| isfreeaccess_bool |
false |
| container_title |
World journal of microbiology and biotechnology |
| authorswithroles_txt_mv |
Rilke, O. @@oth@@ Baum, A. @@oth@@ Weiss, J. @@oth@@ Hommel, R. @@oth@@ Kleber, H. -P. @@oth@@ |
| publishDateDaySort_date |
1992-01-01T00:00:00Z |
| hierarchy_top_id |
NLEJ188986316 |
| id |
NLEJ196253438 |
| language_de |
englisch |
| fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ196253438</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230506161536.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070526s1992 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ196253438</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Kinetics of enzymatic lysis, formation and regeneration of protoplasts ofCandida (Torulopsis) apicola</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1992</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">7</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract The optimal conditions for protoplast formation ofCandida apicola were by using an enzyme fromArthrobacter sp. in combination with 2-mercaptoethanol. The kinetic data support the two-layered structure model of cell wall for this yeast but the structure of the cell wall depended on the age of cells and culture conditions. To regenerate the protoplasts, the type of osmotic stabilizer was important: sorbitol gave 16 to 30% regeneration. Electron microscopy revealed the presence of vesicles in the sections of protoplasts and whole cells ofCandida apicola grown in production medium and producing glycolipids. In sections of whole cells, vesicle-like structures are located in the periplasmic space and in protoplasts they can either be attached to, or released from, the cell surface. These vesicles are thought to be involved in the transport of the surface-active glycolipids and in the protection of the cell against denaturing effects.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Springer Online Journal Archives 1860-2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Rilke, O.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Baum, A.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Weiss, J.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Hommel, R.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Kleber, H. -P.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">World journal of microbiology and biotechnology</subfield><subfield code="d">1985</subfield><subfield code="g">8(1992) vom: Jan., Seite 14-20</subfield><subfield code="w">(DE-627)NLEJ188986316</subfield><subfield code="w">(DE-600)1499109-3</subfield><subfield code="x">1573-0972</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:8</subfield><subfield code="g">year:1992</subfield><subfield code="g">month:01</subfield><subfield code="g">pages:14-20</subfield><subfield code="g">extent:7</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1007/BF01200677</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SOJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">8</subfield><subfield code="j">1992</subfield><subfield code="c">1</subfield><subfield code="h">14-20</subfield><subfield code="g">7</subfield></datafield></record></collection>
|
| series2 |
Springer Online Journal Archives 1860-2002 |
| ppnlink_with_tag_str_mv |
@@773@@(DE-627)NLEJ188986316 |
| format |
electronic Article |
| delete_txt_mv |
keep |
| collection |
NL |
| remote_str |
true |
| illustrated |
Not Illustrated |
| issn |
1573-0972 |
| topic_title |
Kinetics of enzymatic lysis, formation and regeneration of protoplasts ofCandida (Torulopsis) apicola |
| format_facet |
Elektronische Aufsätze Aufsätze Elektronische Ressource |
| format_main_str_mv |
Text Zeitschrift/Artikel |
| carriertype_str_mv |
zu |
| author2_variant |
o r or a b ab j w jw r h rh h - k h- h-k |
| hierarchy_parent_title |
World journal of microbiology and biotechnology |
| hierarchy_parent_id |
NLEJ188986316 |
| hierarchy_top_title |
World journal of microbiology and biotechnology |
| isfreeaccess_txt |
false |
| familylinks_str_mv |
(DE-627)NLEJ188986316 (DE-600)1499109-3 |
| title |
Kinetics of enzymatic lysis, formation and regeneration of protoplasts ofCandida (Torulopsis) apicola |
| spellingShingle |
Kinetics of enzymatic lysis, formation and regeneration of protoplasts ofCandida (Torulopsis) apicola |
| ctrlnum |
(DE-627)NLEJ196253438 |
| title_full |
Kinetics of enzymatic lysis, formation and regeneration of protoplasts ofCandida (Torulopsis) apicola |
| journal |
World journal of microbiology and biotechnology |
| journalStr |
World journal of microbiology and biotechnology |
| lang_code |
eng |
| isOA_bool |
false |
| recordtype |
marc |
| publishDateSort |
1992 |
| contenttype_str_mv |
zzz |
| container_start_page |
14 |
| container_volume |
8 |
| physical |
7 |
| format_se |
Elektronische Aufsätze |
| title_sort |
kinetics of enzymatic lysis, formation and regeneration of protoplasts ofcandida (torulopsis) apicola |
| title_auth |
Kinetics of enzymatic lysis, formation and regeneration of protoplasts ofCandida (Torulopsis) apicola |
| abstract |
Abstract The optimal conditions for protoplast formation ofCandida apicola were by using an enzyme fromArthrobacter sp. in combination with 2-mercaptoethanol. The kinetic data support the two-layered structure model of cell wall for this yeast but the structure of the cell wall depended on the age of cells and culture conditions. To regenerate the protoplasts, the type of osmotic stabilizer was important: sorbitol gave 16 to 30% regeneration. Electron microscopy revealed the presence of vesicles in the sections of protoplasts and whole cells ofCandida apicola grown in production medium and producing glycolipids. In sections of whole cells, vesicle-like structures are located in the periplasmic space and in protoplasts they can either be attached to, or released from, the cell surface. These vesicles are thought to be involved in the transport of the surface-active glycolipids and in the protection of the cell against denaturing effects. |
| abstractGer |
Abstract The optimal conditions for protoplast formation ofCandida apicola were by using an enzyme fromArthrobacter sp. in combination with 2-mercaptoethanol. The kinetic data support the two-layered structure model of cell wall for this yeast but the structure of the cell wall depended on the age of cells and culture conditions. To regenerate the protoplasts, the type of osmotic stabilizer was important: sorbitol gave 16 to 30% regeneration. Electron microscopy revealed the presence of vesicles in the sections of protoplasts and whole cells ofCandida apicola grown in production medium and producing glycolipids. In sections of whole cells, vesicle-like structures are located in the periplasmic space and in protoplasts they can either be attached to, or released from, the cell surface. These vesicles are thought to be involved in the transport of the surface-active glycolipids and in the protection of the cell against denaturing effects. |
| abstract_unstemmed |
Abstract The optimal conditions for protoplast formation ofCandida apicola were by using an enzyme fromArthrobacter sp. in combination with 2-mercaptoethanol. The kinetic data support the two-layered structure model of cell wall for this yeast but the structure of the cell wall depended on the age of cells and culture conditions. To regenerate the protoplasts, the type of osmotic stabilizer was important: sorbitol gave 16 to 30% regeneration. Electron microscopy revealed the presence of vesicles in the sections of protoplasts and whole cells ofCandida apicola grown in production medium and producing glycolipids. In sections of whole cells, vesicle-like structures are located in the periplasmic space and in protoplasts they can either be attached to, or released from, the cell surface. These vesicles are thought to be involved in the transport of the surface-active glycolipids and in the protection of the cell against denaturing effects. |
| collection_details |
GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE |
| title_short |
Kinetics of enzymatic lysis, formation and regeneration of protoplasts ofCandida (Torulopsis) apicola |
| url |
http://dx.doi.org/10.1007/BF01200677 |
| remote_bool |
true |
| author2 |
Rilke, O. Baum, A. Weiss, J. Hommel, R. Kleber, H. -P. |
| author2Str |
Rilke, O. Baum, A. Weiss, J. Hommel, R. Kleber, H. -P. |
| ppnlink |
NLEJ188986316 |
| mediatype_str_mv |
z |
| isOA_txt |
false |
| hochschulschrift_bool |
false |
| author2_role |
oth oth oth oth oth |
| up_date |
2024-07-06T06:02:52.768Z |
| _version_ |
1803808431427026944 |
| fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ196253438</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230506161536.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070526s1992 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ196253438</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Kinetics of enzymatic lysis, formation and regeneration of protoplasts ofCandida (Torulopsis) apicola</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1992</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">7</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract The optimal conditions for protoplast formation ofCandida apicola were by using an enzyme fromArthrobacter sp. in combination with 2-mercaptoethanol. The kinetic data support the two-layered structure model of cell wall for this yeast but the structure of the cell wall depended on the age of cells and culture conditions. To regenerate the protoplasts, the type of osmotic stabilizer was important: sorbitol gave 16 to 30% regeneration. Electron microscopy revealed the presence of vesicles in the sections of protoplasts and whole cells ofCandida apicola grown in production medium and producing glycolipids. In sections of whole cells, vesicle-like structures are located in the periplasmic space and in protoplasts they can either be attached to, or released from, the cell surface. These vesicles are thought to be involved in the transport of the surface-active glycolipids and in the protection of the cell against denaturing effects.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Springer Online Journal Archives 1860-2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Rilke, O.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Baum, A.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Weiss, J.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Hommel, R.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Kleber, H. -P.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">World journal of microbiology and biotechnology</subfield><subfield code="d">1985</subfield><subfield code="g">8(1992) vom: Jan., Seite 14-20</subfield><subfield code="w">(DE-627)NLEJ188986316</subfield><subfield code="w">(DE-600)1499109-3</subfield><subfield code="x">1573-0972</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:8</subfield><subfield code="g">year:1992</subfield><subfield code="g">month:01</subfield><subfield code="g">pages:14-20</subfield><subfield code="g">extent:7</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1007/BF01200677</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SOJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">8</subfield><subfield code="j">1992</subfield><subfield code="c">1</subfield><subfield code="h">14-20</subfield><subfield code="g">7</subfield></datafield></record></collection>
|
| score |
7.3984594 |