Albumen gland of the snail Achatina fulica is the site for synthesis of AchatininH, a sialic acid binding lectin
Abstract A sialic acid binding lectin, AchatininH was purified from the hemolymph of Achatina fulica snail. To identify the site of synthesis of AchatininH, in vitro incubation studies in presence of labelled amino acid precursor were performed. Different organs from the snail were sliced and incuba...
Ausführliche Beschreibung
Gespeichert in:
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1992 |
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| Umfang: |
6 |
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| Reproduktion: |
Springer Online Journal Archives 1860-2002 |
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| Übergeordnetes Werk: |
in: Molecular and cellular biochemistry - 1973, 117(1992) vom: Feb., Seite 133-138 |
| Übergeordnetes Werk: |
volume:117 ; year:1992 ; month:02 ; pages:133-138 ; extent:6 |
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| 245 | 1 | 0 | |a Albumen gland of the snail Achatina fulica is the site for synthesis of AchatininH, a sialic acid binding lectin |
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| 520 | |a Abstract A sialic acid binding lectin, AchatininH was purified from the hemolymph of Achatina fulica snail. To identify the site of synthesis of AchatininH, in vitro incubation studies in presence of labelled amino acid precursor were performed. Different organs from the snail were sliced and incubated in methionine-deficient Eagle's minimum essential medium containing [35S] - methionine at 25° C for 5 h. After termination of incubation, tissues were homogenized, centrifuged and the de novo synthesized protein was immunoprecipitated with specific AchatininH antibody, followed by protein-A. The precipitated antigen-antibody complex was analysed by SDS-PAGE. Data obtained from native gel electrophoresis and SDS-PAGE radioautographic analysis indicates that AchatininH is synthesized in the albumen gland. | ||
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| 700 | 1 | |a Chowdhury, Mridula |4 oth | |
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(DE-627)NLEJ198027591 DE-627 ger DE-627 rakwb eng Albumen gland of the snail Achatina fulica is the site for synthesis of AchatininH, a sialic acid binding lectin 1992 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract A sialic acid binding lectin, AchatininH was purified from the hemolymph of Achatina fulica snail. To identify the site of synthesis of AchatininH, in vitro incubation studies in presence of labelled amino acid precursor were performed. Different organs from the snail were sliced and incubated in methionine-deficient Eagle's minimum essential medium containing [35S] - methionine at 25° C for 5 h. After termination of incubation, tissues were homogenized, centrifuged and the de novo synthesized protein was immunoprecipitated with specific AchatininH antibody, followed by protein-A. The precipitated antigen-antibody complex was analysed by SDS-PAGE. Data obtained from native gel electrophoresis and SDS-PAGE radioautographic analysis indicates that AchatininH is synthesized in the albumen gland. Springer Online Journal Archives 1860-2002 Sen, Goutam oth Mandal, Chitra oth Chowdhury, Mridula oth in Molecular and cellular biochemistry 1973 117(1992) vom: Feb., Seite 133-138 (DE-627)NLEJ188989099 (DE-600)2003615-2 1573-4919 nnns volume:117 year:1992 month:02 pages:133-138 extent:6 http://dx.doi.org/10.1007/BF00230752 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 117 1992 2 133-138 6 |
| spelling |
(DE-627)NLEJ198027591 DE-627 ger DE-627 rakwb eng Albumen gland of the snail Achatina fulica is the site for synthesis of AchatininH, a sialic acid binding lectin 1992 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract A sialic acid binding lectin, AchatininH was purified from the hemolymph of Achatina fulica snail. To identify the site of synthesis of AchatininH, in vitro incubation studies in presence of labelled amino acid precursor were performed. Different organs from the snail were sliced and incubated in methionine-deficient Eagle's minimum essential medium containing [35S] - methionine at 25° C for 5 h. After termination of incubation, tissues were homogenized, centrifuged and the de novo synthesized protein was immunoprecipitated with specific AchatininH antibody, followed by protein-A. The precipitated antigen-antibody complex was analysed by SDS-PAGE. Data obtained from native gel electrophoresis and SDS-PAGE radioautographic analysis indicates that AchatininH is synthesized in the albumen gland. Springer Online Journal Archives 1860-2002 Sen, Goutam oth Mandal, Chitra oth Chowdhury, Mridula oth in Molecular and cellular biochemistry 1973 117(1992) vom: Feb., Seite 133-138 (DE-627)NLEJ188989099 (DE-600)2003615-2 1573-4919 nnns volume:117 year:1992 month:02 pages:133-138 extent:6 http://dx.doi.org/10.1007/BF00230752 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 117 1992 2 133-138 6 |
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(DE-627)NLEJ198027591 DE-627 ger DE-627 rakwb eng Albumen gland of the snail Achatina fulica is the site for synthesis of AchatininH, a sialic acid binding lectin 1992 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract A sialic acid binding lectin, AchatininH was purified from the hemolymph of Achatina fulica snail. To identify the site of synthesis of AchatininH, in vitro incubation studies in presence of labelled amino acid precursor were performed. Different organs from the snail were sliced and incubated in methionine-deficient Eagle's minimum essential medium containing [35S] - methionine at 25° C for 5 h. After termination of incubation, tissues were homogenized, centrifuged and the de novo synthesized protein was immunoprecipitated with specific AchatininH antibody, followed by protein-A. The precipitated antigen-antibody complex was analysed by SDS-PAGE. Data obtained from native gel electrophoresis and SDS-PAGE radioautographic analysis indicates that AchatininH is synthesized in the albumen gland. Springer Online Journal Archives 1860-2002 Sen, Goutam oth Mandal, Chitra oth Chowdhury, Mridula oth in Molecular and cellular biochemistry 1973 117(1992) vom: Feb., Seite 133-138 (DE-627)NLEJ188989099 (DE-600)2003615-2 1573-4919 nnns volume:117 year:1992 month:02 pages:133-138 extent:6 http://dx.doi.org/10.1007/BF00230752 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 117 1992 2 133-138 6 |
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(DE-627)NLEJ198027591 DE-627 ger DE-627 rakwb eng Albumen gland of the snail Achatina fulica is the site for synthesis of AchatininH, a sialic acid binding lectin 1992 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract A sialic acid binding lectin, AchatininH was purified from the hemolymph of Achatina fulica snail. To identify the site of synthesis of AchatininH, in vitro incubation studies in presence of labelled amino acid precursor were performed. Different organs from the snail were sliced and incubated in methionine-deficient Eagle's minimum essential medium containing [35S] - methionine at 25° C for 5 h. After termination of incubation, tissues were homogenized, centrifuged and the de novo synthesized protein was immunoprecipitated with specific AchatininH antibody, followed by protein-A. The precipitated antigen-antibody complex was analysed by SDS-PAGE. Data obtained from native gel electrophoresis and SDS-PAGE radioautographic analysis indicates that AchatininH is synthesized in the albumen gland. Springer Online Journal Archives 1860-2002 Sen, Goutam oth Mandal, Chitra oth Chowdhury, Mridula oth in Molecular and cellular biochemistry 1973 117(1992) vom: Feb., Seite 133-138 (DE-627)NLEJ188989099 (DE-600)2003615-2 1573-4919 nnns volume:117 year:1992 month:02 pages:133-138 extent:6 http://dx.doi.org/10.1007/BF00230752 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 117 1992 2 133-138 6 |
| allfieldsSound |
(DE-627)NLEJ198027591 DE-627 ger DE-627 rakwb eng Albumen gland of the snail Achatina fulica is the site for synthesis of AchatininH, a sialic acid binding lectin 1992 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract A sialic acid binding lectin, AchatininH was purified from the hemolymph of Achatina fulica snail. To identify the site of synthesis of AchatininH, in vitro incubation studies in presence of labelled amino acid precursor were performed. Different organs from the snail were sliced and incubated in methionine-deficient Eagle's minimum essential medium containing [35S] - methionine at 25° C for 5 h. After termination of incubation, tissues were homogenized, centrifuged and the de novo synthesized protein was immunoprecipitated with specific AchatininH antibody, followed by protein-A. The precipitated antigen-antibody complex was analysed by SDS-PAGE. Data obtained from native gel electrophoresis and SDS-PAGE radioautographic analysis indicates that AchatininH is synthesized in the albumen gland. Springer Online Journal Archives 1860-2002 Sen, Goutam oth Mandal, Chitra oth Chowdhury, Mridula oth in Molecular and cellular biochemistry 1973 117(1992) vom: Feb., Seite 133-138 (DE-627)NLEJ188989099 (DE-600)2003615-2 1573-4919 nnns volume:117 year:1992 month:02 pages:133-138 extent:6 http://dx.doi.org/10.1007/BF00230752 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 117 1992 2 133-138 6 |
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in Molecular and cellular biochemistry 117(1992) vom: Feb., Seite 133-138 volume:117 year:1992 month:02 pages:133-138 extent:6 |
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albumen gland of the snail achatina fulica is the site for synthesis of achatininh, a sialic acid binding lectin |
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Albumen gland of the snail Achatina fulica is the site for synthesis of AchatininH, a sialic acid binding lectin |
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Abstract A sialic acid binding lectin, AchatininH was purified from the hemolymph of Achatina fulica snail. To identify the site of synthesis of AchatininH, in vitro incubation studies in presence of labelled amino acid precursor were performed. Different organs from the snail were sliced and incubated in methionine-deficient Eagle's minimum essential medium containing [35S] - methionine at 25° C for 5 h. After termination of incubation, tissues were homogenized, centrifuged and the de novo synthesized protein was immunoprecipitated with specific AchatininH antibody, followed by protein-A. The precipitated antigen-antibody complex was analysed by SDS-PAGE. Data obtained from native gel electrophoresis and SDS-PAGE radioautographic analysis indicates that AchatininH is synthesized in the albumen gland. |
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Abstract A sialic acid binding lectin, AchatininH was purified from the hemolymph of Achatina fulica snail. To identify the site of synthesis of AchatininH, in vitro incubation studies in presence of labelled amino acid precursor were performed. Different organs from the snail were sliced and incubated in methionine-deficient Eagle's minimum essential medium containing [35S] - methionine at 25° C for 5 h. After termination of incubation, tissues were homogenized, centrifuged and the de novo synthesized protein was immunoprecipitated with specific AchatininH antibody, followed by protein-A. The precipitated antigen-antibody complex was analysed by SDS-PAGE. Data obtained from native gel electrophoresis and SDS-PAGE radioautographic analysis indicates that AchatininH is synthesized in the albumen gland. |
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Abstract A sialic acid binding lectin, AchatininH was purified from the hemolymph of Achatina fulica snail. To identify the site of synthesis of AchatininH, in vitro incubation studies in presence of labelled amino acid precursor were performed. Different organs from the snail were sliced and incubated in methionine-deficient Eagle's minimum essential medium containing [35S] - methionine at 25° C for 5 h. After termination of incubation, tissues were homogenized, centrifuged and the de novo synthesized protein was immunoprecipitated with specific AchatininH antibody, followed by protein-A. The precipitated antigen-antibody complex was analysed by SDS-PAGE. Data obtained from native gel electrophoresis and SDS-PAGE radioautographic analysis indicates that AchatininH is synthesized in the albumen gland. |
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