Detection of various forms of brain myelin basic protein in vertebrates by electroimmunoblotting
Abstract An electroimmunoblot technique was used to detect various forms of myelin basic protein (MBP) in brain homogenates of 14 vertebrate species. Three antibodies were used to probe the immunoblots: a monoclonal anti-human MPB reacting with an antigenic determinant located at amino acid residues...
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1984 |
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| Umfang: |
11 |
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| Reproduktion: |
Springer Online Journal Archives 1860-2002 |
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| Übergeordnetes Werk: |
in: Neurochemical research - 1976, 9(1984) vom: Okt., Seite 1359-1369 |
| Übergeordnetes Werk: |
volume:9 ; year:1984 ; month:10 ; pages:1359-1369 ; extent:11 |
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NLEJ198259077 |
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| 245 | 1 | 0 | |a Detection of various forms of brain myelin basic protein in vertebrates by electroimmunoblotting |
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| 520 | |a Abstract An electroimmunoblot technique was used to detect various forms of myelin basic protein (MBP) in brain homogenates of 14 vertebrate species. Three antibodies were used to probe the immunoblots: a monoclonal anti-human MPB reacting with an antigenic determinant located at amino acid residues 131 to 136; a polyclonal anti-human MBP and a polyclonal anti-chicken MBP. Because no processing of the tissue is required prior to electrophoresis, in vitro artifacts are minimized. The 18.5 K form of MBP was present in all species except the shark. A 21.5 K MBP was observed in ovine, bovine, pig, rabbit, mouse, rat, monkey, but not in human, guinea pig, shark, toad and marsupial brains. A variant with a molecular weight between 17 K and 18 K was found in mouse, rat, bovine, human, monkey, pig, and chicken brains, and was the sole component in the shark brain. Marsupial brains had five or six forms of MBP between 14.5 K and 18.5 K. | ||
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| 700 | 1 | |a Carnegie, Patrick R. |4 oth | |
| 700 | 1 | |a Bernard, Claude C. A. |4 oth | |
| 700 | 1 | |a Scott Linthicum, D. |4 oth | |
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(DE-627)NLEJ198259077 DE-627 ger DE-627 rakwb eng Detection of various forms of brain myelin basic protein in vertebrates by electroimmunoblotting 1984 11 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract An electroimmunoblot technique was used to detect various forms of myelin basic protein (MBP) in brain homogenates of 14 vertebrate species. Three antibodies were used to probe the immunoblots: a monoclonal anti-human MPB reacting with an antigenic determinant located at amino acid residues 131 to 136; a polyclonal anti-human MBP and a polyclonal anti-chicken MBP. Because no processing of the tissue is required prior to electrophoresis, in vitro artifacts are minimized. The 18.5 K form of MBP was present in all species except the shark. A 21.5 K MBP was observed in ovine, bovine, pig, rabbit, mouse, rat, monkey, but not in human, guinea pig, shark, toad and marsupial brains. A variant with a molecular weight between 17 K and 18 K was found in mouse, rat, bovine, human, monkey, pig, and chicken brains, and was the sole component in the shark brain. Marsupial brains had five or six forms of MBP between 14.5 K and 18.5 K. Springer Online Journal Archives 1860-2002 Kerlero De Rosbo, Nicole oth Carnegie, Patrick R. oth Bernard, Claude C. A. oth Scott Linthicum, D. oth in Neurochemical research 1976 9(1984) vom: Okt., Seite 1359-1369 (DE-627)NLEJ188985557 (DE-600)2018503-0 1573-6903 nnns volume:9 year:1984 month:10 pages:1359-1369 extent:11 http://dx.doi.org/10.1007/BF00964663 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 9 1984 10 1359-1369 11 |
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(DE-627)NLEJ198259077 DE-627 ger DE-627 rakwb eng Detection of various forms of brain myelin basic protein in vertebrates by electroimmunoblotting 1984 11 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract An electroimmunoblot technique was used to detect various forms of myelin basic protein (MBP) in brain homogenates of 14 vertebrate species. Three antibodies were used to probe the immunoblots: a monoclonal anti-human MPB reacting with an antigenic determinant located at amino acid residues 131 to 136; a polyclonal anti-human MBP and a polyclonal anti-chicken MBP. Because no processing of the tissue is required prior to electrophoresis, in vitro artifacts are minimized. The 18.5 K form of MBP was present in all species except the shark. A 21.5 K MBP was observed in ovine, bovine, pig, rabbit, mouse, rat, monkey, but not in human, guinea pig, shark, toad and marsupial brains. A variant with a molecular weight between 17 K and 18 K was found in mouse, rat, bovine, human, monkey, pig, and chicken brains, and was the sole component in the shark brain. Marsupial brains had five or six forms of MBP between 14.5 K and 18.5 K. Springer Online Journal Archives 1860-2002 Kerlero De Rosbo, Nicole oth Carnegie, Patrick R. oth Bernard, Claude C. A. oth Scott Linthicum, D. oth in Neurochemical research 1976 9(1984) vom: Okt., Seite 1359-1369 (DE-627)NLEJ188985557 (DE-600)2018503-0 1573-6903 nnns volume:9 year:1984 month:10 pages:1359-1369 extent:11 http://dx.doi.org/10.1007/BF00964663 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 9 1984 10 1359-1369 11 |
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(DE-627)NLEJ198259077 DE-627 ger DE-627 rakwb eng Detection of various forms of brain myelin basic protein in vertebrates by electroimmunoblotting 1984 11 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract An electroimmunoblot technique was used to detect various forms of myelin basic protein (MBP) in brain homogenates of 14 vertebrate species. Three antibodies were used to probe the immunoblots: a monoclonal anti-human MPB reacting with an antigenic determinant located at amino acid residues 131 to 136; a polyclonal anti-human MBP and a polyclonal anti-chicken MBP. Because no processing of the tissue is required prior to electrophoresis, in vitro artifacts are minimized. The 18.5 K form of MBP was present in all species except the shark. A 21.5 K MBP was observed in ovine, bovine, pig, rabbit, mouse, rat, monkey, but not in human, guinea pig, shark, toad and marsupial brains. A variant with a molecular weight between 17 K and 18 K was found in mouse, rat, bovine, human, monkey, pig, and chicken brains, and was the sole component in the shark brain. Marsupial brains had five or six forms of MBP between 14.5 K and 18.5 K. Springer Online Journal Archives 1860-2002 Kerlero De Rosbo, Nicole oth Carnegie, Patrick R. oth Bernard, Claude C. A. oth Scott Linthicum, D. oth in Neurochemical research 1976 9(1984) vom: Okt., Seite 1359-1369 (DE-627)NLEJ188985557 (DE-600)2018503-0 1573-6903 nnns volume:9 year:1984 month:10 pages:1359-1369 extent:11 http://dx.doi.org/10.1007/BF00964663 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 9 1984 10 1359-1369 11 |
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(DE-627)NLEJ198259077 DE-627 ger DE-627 rakwb eng Detection of various forms of brain myelin basic protein in vertebrates by electroimmunoblotting 1984 11 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract An electroimmunoblot technique was used to detect various forms of myelin basic protein (MBP) in brain homogenates of 14 vertebrate species. Three antibodies were used to probe the immunoblots: a monoclonal anti-human MPB reacting with an antigenic determinant located at amino acid residues 131 to 136; a polyclonal anti-human MBP and a polyclonal anti-chicken MBP. Because no processing of the tissue is required prior to electrophoresis, in vitro artifacts are minimized. The 18.5 K form of MBP was present in all species except the shark. A 21.5 K MBP was observed in ovine, bovine, pig, rabbit, mouse, rat, monkey, but not in human, guinea pig, shark, toad and marsupial brains. A variant with a molecular weight between 17 K and 18 K was found in mouse, rat, bovine, human, monkey, pig, and chicken brains, and was the sole component in the shark brain. Marsupial brains had five or six forms of MBP between 14.5 K and 18.5 K. Springer Online Journal Archives 1860-2002 Kerlero De Rosbo, Nicole oth Carnegie, Patrick R. oth Bernard, Claude C. A. oth Scott Linthicum, D. oth in Neurochemical research 1976 9(1984) vom: Okt., Seite 1359-1369 (DE-627)NLEJ188985557 (DE-600)2018503-0 1573-6903 nnns volume:9 year:1984 month:10 pages:1359-1369 extent:11 http://dx.doi.org/10.1007/BF00964663 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 9 1984 10 1359-1369 11 |
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(DE-627)NLEJ198259077 DE-627 ger DE-627 rakwb eng Detection of various forms of brain myelin basic protein in vertebrates by electroimmunoblotting 1984 11 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract An electroimmunoblot technique was used to detect various forms of myelin basic protein (MBP) in brain homogenates of 14 vertebrate species. Three antibodies were used to probe the immunoblots: a monoclonal anti-human MPB reacting with an antigenic determinant located at amino acid residues 131 to 136; a polyclonal anti-human MBP and a polyclonal anti-chicken MBP. Because no processing of the tissue is required prior to electrophoresis, in vitro artifacts are minimized. The 18.5 K form of MBP was present in all species except the shark. A 21.5 K MBP was observed in ovine, bovine, pig, rabbit, mouse, rat, monkey, but not in human, guinea pig, shark, toad and marsupial brains. A variant with a molecular weight between 17 K and 18 K was found in mouse, rat, bovine, human, monkey, pig, and chicken brains, and was the sole component in the shark brain. Marsupial brains had five or six forms of MBP between 14.5 K and 18.5 K. Springer Online Journal Archives 1860-2002 Kerlero De Rosbo, Nicole oth Carnegie, Patrick R. oth Bernard, Claude C. A. oth Scott Linthicum, D. oth in Neurochemical research 1976 9(1984) vom: Okt., Seite 1359-1369 (DE-627)NLEJ188985557 (DE-600)2018503-0 1573-6903 nnns volume:9 year:1984 month:10 pages:1359-1369 extent:11 http://dx.doi.org/10.1007/BF00964663 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 9 1984 10 1359-1369 11 |
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detection of various forms of brain myelin basic protein in vertebrates by electroimmunoblotting |
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Detection of various forms of brain myelin basic protein in vertebrates by electroimmunoblotting |
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Abstract An electroimmunoblot technique was used to detect various forms of myelin basic protein (MBP) in brain homogenates of 14 vertebrate species. Three antibodies were used to probe the immunoblots: a monoclonal anti-human MPB reacting with an antigenic determinant located at amino acid residues 131 to 136; a polyclonal anti-human MBP and a polyclonal anti-chicken MBP. Because no processing of the tissue is required prior to electrophoresis, in vitro artifacts are minimized. The 18.5 K form of MBP was present in all species except the shark. A 21.5 K MBP was observed in ovine, bovine, pig, rabbit, mouse, rat, monkey, but not in human, guinea pig, shark, toad and marsupial brains. A variant with a molecular weight between 17 K and 18 K was found in mouse, rat, bovine, human, monkey, pig, and chicken brains, and was the sole component in the shark brain. Marsupial brains had five or six forms of MBP between 14.5 K and 18.5 K. |
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Abstract An electroimmunoblot technique was used to detect various forms of myelin basic protein (MBP) in brain homogenates of 14 vertebrate species. Three antibodies were used to probe the immunoblots: a monoclonal anti-human MPB reacting with an antigenic determinant located at amino acid residues 131 to 136; a polyclonal anti-human MBP and a polyclonal anti-chicken MBP. Because no processing of the tissue is required prior to electrophoresis, in vitro artifacts are minimized. The 18.5 K form of MBP was present in all species except the shark. A 21.5 K MBP was observed in ovine, bovine, pig, rabbit, mouse, rat, monkey, but not in human, guinea pig, shark, toad and marsupial brains. A variant with a molecular weight between 17 K and 18 K was found in mouse, rat, bovine, human, monkey, pig, and chicken brains, and was the sole component in the shark brain. Marsupial brains had five or six forms of MBP between 14.5 K and 18.5 K. |
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Abstract An electroimmunoblot technique was used to detect various forms of myelin basic protein (MBP) in brain homogenates of 14 vertebrate species. Three antibodies were used to probe the immunoblots: a monoclonal anti-human MPB reacting with an antigenic determinant located at amino acid residues 131 to 136; a polyclonal anti-human MBP and a polyclonal anti-chicken MBP. Because no processing of the tissue is required prior to electrophoresis, in vitro artifacts are minimized. The 18.5 K form of MBP was present in all species except the shark. A 21.5 K MBP was observed in ovine, bovine, pig, rabbit, mouse, rat, monkey, but not in human, guinea pig, shark, toad and marsupial brains. A variant with a molecular weight between 17 K and 18 K was found in mouse, rat, bovine, human, monkey, pig, and chicken brains, and was the sole component in the shark brain. Marsupial brains had five or six forms of MBP between 14.5 K and 18.5 K. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ198259077</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210705234948.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070527s1984 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ198259077</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Detection of various forms of brain myelin basic protein in vertebrates by electroimmunoblotting</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1984</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">11</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract An electroimmunoblot technique was used to detect various forms of myelin basic protein (MBP) in brain homogenates of 14 vertebrate species. Three antibodies were used to probe the immunoblots: a monoclonal anti-human MPB reacting with an antigenic determinant located at amino acid residues 131 to 136; a polyclonal anti-human MBP and a polyclonal anti-chicken MBP. Because no processing of the tissue is required prior to electrophoresis, in vitro artifacts are minimized. The 18.5 K form of MBP was present in all species except the shark. A 21.5 K MBP was observed in ovine, bovine, pig, rabbit, mouse, rat, monkey, but not in human, guinea pig, shark, toad and marsupial brains. A variant with a molecular weight between 17 K and 18 K was found in mouse, rat, bovine, human, monkey, pig, and chicken brains, and was the sole component in the shark brain. Marsupial brains had five or six forms of MBP between 14.5 K and 18.5 K.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Springer Online Journal Archives 1860-2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Kerlero De Rosbo, Nicole</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Carnegie, Patrick R.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Bernard, Claude C. A.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Scott Linthicum, D.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">Neurochemical research</subfield><subfield code="d">1976</subfield><subfield code="g">9(1984) vom: Okt., Seite 1359-1369</subfield><subfield code="w">(DE-627)NLEJ188985557</subfield><subfield code="w">(DE-600)2018503-0</subfield><subfield code="x">1573-6903</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:9</subfield><subfield code="g">year:1984</subfield><subfield code="g">month:10</subfield><subfield code="g">pages:1359-1369</subfield><subfield code="g">extent:11</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1007/BF00964663</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SOJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">9</subfield><subfield code="j">1984</subfield><subfield code="c">10</subfield><subfield code="h">1359-1369</subfield><subfield code="g">11</subfield></datafield></record></collection>
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