Regulation of volume reabsorption by thyroid hormones in the proximal tubule of rat: Minor role of luminal sodium permeability
Abstract In order to investigate whether changes in luminal membrane sodium permeability can explain the increase in isotonic fluid reabsorption (J v) found in proximal tubules of thyroidectomized rats (TX) treated with tri-iodothyronine (T3), experiments were carried out on TX rats and TX rats trea...
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E-Artikel |
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Englisch |
| Erschienen: |
1985 |
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8 |
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Springer Online Journal Archives 1860-2002 |
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in: Pflügers Archiv - 1868, 403(1985) vom: Jan., Seite 97-104 |
| Übergeordnetes Werk: |
volume:403 ; year:1985 ; month:01 ; pages:97-104 ; extent:8 |
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| 520 | |a Abstract In order to investigate whether changes in luminal membrane sodium permeability can explain the increase in isotonic fluid reabsorption (J v) found in proximal tubules of thyroidectomized rats (TX) treated with tri-iodothyronine (T3), experiments were carried out on TX rats and TX rats treated for 3 days (TX+T3) with physiological doses (10 μg/kg body wt) of T3. Two sets of experiments were performed: 1) in vivo, using the micropuncture technique for the measurements ofJ v; 2) in vitro, using isolated brush border membrane vesicles for the direct measurement of Na+ permeability. In micropuncture studies a 65% increase inJ v of TX rats was observed after treatment with T3. Luminal perfusion of proximal tubules of TX rats with Amphotericin B (10 μg/ml), to increase luminal sodium permeability, enhancedJ v only by 15%. Brush border membrane vesicles isolated from TX and TX+T3 rats showed the same sodium permeability in uptake or efflux experiments. These results were confirmed by the fact that sodium gradient dependent histidine transport into brush border membrane vesicles did not change after T3 treatment. Finally, measuring the amiloride sensitive sodium uptake, it was also found that the Na+−H+ exchange was also only slightly affected by T3. These micropuncture and vesicle data indicate that the large effect of T3 on the trans-cellular sodium transport and volume reabsorption in the proximal tubule, cannot be explained by an action of T3 on the sodium entry step across the brush border membrane. | ||
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(DE-627)NLEJ200978195 DE-627 ger DE-627 rakwb eng Regulation of volume reabsorption by thyroid hormones in the proximal tubule of rat: Minor role of luminal sodium permeability 1985 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract In order to investigate whether changes in luminal membrane sodium permeability can explain the increase in isotonic fluid reabsorption (J v) found in proximal tubules of thyroidectomized rats (TX) treated with tri-iodothyronine (T3), experiments were carried out on TX rats and TX rats treated for 3 days (TX+T3) with physiological doses (10 μg/kg body wt) of T3. Two sets of experiments were performed: 1) in vivo, using the micropuncture technique for the measurements ofJ v; 2) in vitro, using isolated brush border membrane vesicles for the direct measurement of Na+ permeability. In micropuncture studies a 65% increase inJ v of TX rats was observed after treatment with T3. Luminal perfusion of proximal tubules of TX rats with Amphotericin B (10 μg/ml), to increase luminal sodium permeability, enhancedJ v only by 15%. Brush border membrane vesicles isolated from TX and TX+T3 rats showed the same sodium permeability in uptake or efflux experiments. These results were confirmed by the fact that sodium gradient dependent histidine transport into brush border membrane vesicles did not change after T3 treatment. Finally, measuring the amiloride sensitive sodium uptake, it was also found that the Na+−H+ exchange was also only slightly affected by T3. These micropuncture and vesicle data indicate that the large effect of T3 on the trans-cellular sodium transport and volume reabsorption in the proximal tubule, cannot be explained by an action of T3 on the sodium entry step across the brush border membrane. Springer Online Journal Archives 1860-2002 Capasso, Giovambattista oth Kinne-Saffran, Eva oth Santo, Natale G. oth Kinne, Rolf oth in Pflügers Archiv 1868 403(1985) vom: Jan., Seite 97-104 (DE-627)NLEJ188987363 (DE-600)1463014-x 1432-2013 nnns volume:403 year:1985 month:01 pages:97-104 extent:8 http://dx.doi.org/10.1007/BF00583288 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 403 1985 1 97-104 8 |
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(DE-627)NLEJ200978195 DE-627 ger DE-627 rakwb eng Regulation of volume reabsorption by thyroid hormones in the proximal tubule of rat: Minor role of luminal sodium permeability 1985 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract In order to investigate whether changes in luminal membrane sodium permeability can explain the increase in isotonic fluid reabsorption (J v) found in proximal tubules of thyroidectomized rats (TX) treated with tri-iodothyronine (T3), experiments were carried out on TX rats and TX rats treated for 3 days (TX+T3) with physiological doses (10 μg/kg body wt) of T3. Two sets of experiments were performed: 1) in vivo, using the micropuncture technique for the measurements ofJ v; 2) in vitro, using isolated brush border membrane vesicles for the direct measurement of Na+ permeability. In micropuncture studies a 65% increase inJ v of TX rats was observed after treatment with T3. Luminal perfusion of proximal tubules of TX rats with Amphotericin B (10 μg/ml), to increase luminal sodium permeability, enhancedJ v only by 15%. Brush border membrane vesicles isolated from TX and TX+T3 rats showed the same sodium permeability in uptake or efflux experiments. These results were confirmed by the fact that sodium gradient dependent histidine transport into brush border membrane vesicles did not change after T3 treatment. Finally, measuring the amiloride sensitive sodium uptake, it was also found that the Na+−H+ exchange was also only slightly affected by T3. These micropuncture and vesicle data indicate that the large effect of T3 on the trans-cellular sodium transport and volume reabsorption in the proximal tubule, cannot be explained by an action of T3 on the sodium entry step across the brush border membrane. Springer Online Journal Archives 1860-2002 Capasso, Giovambattista oth Kinne-Saffran, Eva oth Santo, Natale G. oth Kinne, Rolf oth in Pflügers Archiv 1868 403(1985) vom: Jan., Seite 97-104 (DE-627)NLEJ188987363 (DE-600)1463014-x 1432-2013 nnns volume:403 year:1985 month:01 pages:97-104 extent:8 http://dx.doi.org/10.1007/BF00583288 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 403 1985 1 97-104 8 |
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(DE-627)NLEJ200978195 DE-627 ger DE-627 rakwb eng Regulation of volume reabsorption by thyroid hormones in the proximal tubule of rat: Minor role of luminal sodium permeability 1985 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract In order to investigate whether changes in luminal membrane sodium permeability can explain the increase in isotonic fluid reabsorption (J v) found in proximal tubules of thyroidectomized rats (TX) treated with tri-iodothyronine (T3), experiments were carried out on TX rats and TX rats treated for 3 days (TX+T3) with physiological doses (10 μg/kg body wt) of T3. Two sets of experiments were performed: 1) in vivo, using the micropuncture technique for the measurements ofJ v; 2) in vitro, using isolated brush border membrane vesicles for the direct measurement of Na+ permeability. In micropuncture studies a 65% increase inJ v of TX rats was observed after treatment with T3. Luminal perfusion of proximal tubules of TX rats with Amphotericin B (10 μg/ml), to increase luminal sodium permeability, enhancedJ v only by 15%. Brush border membrane vesicles isolated from TX and TX+T3 rats showed the same sodium permeability in uptake or efflux experiments. These results were confirmed by the fact that sodium gradient dependent histidine transport into brush border membrane vesicles did not change after T3 treatment. Finally, measuring the amiloride sensitive sodium uptake, it was also found that the Na+−H+ exchange was also only slightly affected by T3. These micropuncture and vesicle data indicate that the large effect of T3 on the trans-cellular sodium transport and volume reabsorption in the proximal tubule, cannot be explained by an action of T3 on the sodium entry step across the brush border membrane. Springer Online Journal Archives 1860-2002 Capasso, Giovambattista oth Kinne-Saffran, Eva oth Santo, Natale G. oth Kinne, Rolf oth in Pflügers Archiv 1868 403(1985) vom: Jan., Seite 97-104 (DE-627)NLEJ188987363 (DE-600)1463014-x 1432-2013 nnns volume:403 year:1985 month:01 pages:97-104 extent:8 http://dx.doi.org/10.1007/BF00583288 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 403 1985 1 97-104 8 |
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(DE-627)NLEJ200978195 DE-627 ger DE-627 rakwb eng Regulation of volume reabsorption by thyroid hormones in the proximal tubule of rat: Minor role of luminal sodium permeability 1985 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract In order to investigate whether changes in luminal membrane sodium permeability can explain the increase in isotonic fluid reabsorption (J v) found in proximal tubules of thyroidectomized rats (TX) treated with tri-iodothyronine (T3), experiments were carried out on TX rats and TX rats treated for 3 days (TX+T3) with physiological doses (10 μg/kg body wt) of T3. Two sets of experiments were performed: 1) in vivo, using the micropuncture technique for the measurements ofJ v; 2) in vitro, using isolated brush border membrane vesicles for the direct measurement of Na+ permeability. In micropuncture studies a 65% increase inJ v of TX rats was observed after treatment with T3. Luminal perfusion of proximal tubules of TX rats with Amphotericin B (10 μg/ml), to increase luminal sodium permeability, enhancedJ v only by 15%. Brush border membrane vesicles isolated from TX and TX+T3 rats showed the same sodium permeability in uptake or efflux experiments. These results were confirmed by the fact that sodium gradient dependent histidine transport into brush border membrane vesicles did not change after T3 treatment. Finally, measuring the amiloride sensitive sodium uptake, it was also found that the Na+−H+ exchange was also only slightly affected by T3. These micropuncture and vesicle data indicate that the large effect of T3 on the trans-cellular sodium transport and volume reabsorption in the proximal tubule, cannot be explained by an action of T3 on the sodium entry step across the brush border membrane. Springer Online Journal Archives 1860-2002 Capasso, Giovambattista oth Kinne-Saffran, Eva oth Santo, Natale G. oth Kinne, Rolf oth in Pflügers Archiv 1868 403(1985) vom: Jan., Seite 97-104 (DE-627)NLEJ188987363 (DE-600)1463014-x 1432-2013 nnns volume:403 year:1985 month:01 pages:97-104 extent:8 http://dx.doi.org/10.1007/BF00583288 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 403 1985 1 97-104 8 |
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(DE-627)NLEJ200978195 DE-627 ger DE-627 rakwb eng Regulation of volume reabsorption by thyroid hormones in the proximal tubule of rat: Minor role of luminal sodium permeability 1985 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract In order to investigate whether changes in luminal membrane sodium permeability can explain the increase in isotonic fluid reabsorption (J v) found in proximal tubules of thyroidectomized rats (TX) treated with tri-iodothyronine (T3), experiments were carried out on TX rats and TX rats treated for 3 days (TX+T3) with physiological doses (10 μg/kg body wt) of T3. Two sets of experiments were performed: 1) in vivo, using the micropuncture technique for the measurements ofJ v; 2) in vitro, using isolated brush border membrane vesicles for the direct measurement of Na+ permeability. In micropuncture studies a 65% increase inJ v of TX rats was observed after treatment with T3. Luminal perfusion of proximal tubules of TX rats with Amphotericin B (10 μg/ml), to increase luminal sodium permeability, enhancedJ v only by 15%. Brush border membrane vesicles isolated from TX and TX+T3 rats showed the same sodium permeability in uptake or efflux experiments. These results were confirmed by the fact that sodium gradient dependent histidine transport into brush border membrane vesicles did not change after T3 treatment. Finally, measuring the amiloride sensitive sodium uptake, it was also found that the Na+−H+ exchange was also only slightly affected by T3. These micropuncture and vesicle data indicate that the large effect of T3 on the trans-cellular sodium transport and volume reabsorption in the proximal tubule, cannot be explained by an action of T3 on the sodium entry step across the brush border membrane. Springer Online Journal Archives 1860-2002 Capasso, Giovambattista oth Kinne-Saffran, Eva oth Santo, Natale G. oth Kinne, Rolf oth in Pflügers Archiv 1868 403(1985) vom: Jan., Seite 97-104 (DE-627)NLEJ188987363 (DE-600)1463014-x 1432-2013 nnns volume:403 year:1985 month:01 pages:97-104 extent:8 http://dx.doi.org/10.1007/BF00583288 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 403 1985 1 97-104 8 |
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regulation of volume reabsorption by thyroid hormones in the proximal tubule of rat: minor role of luminal sodium permeability |
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Regulation of volume reabsorption by thyroid hormones in the proximal tubule of rat: Minor role of luminal sodium permeability |
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Abstract In order to investigate whether changes in luminal membrane sodium permeability can explain the increase in isotonic fluid reabsorption (J v) found in proximal tubules of thyroidectomized rats (TX) treated with tri-iodothyronine (T3), experiments were carried out on TX rats and TX rats treated for 3 days (TX+T3) with physiological doses (10 μg/kg body wt) of T3. Two sets of experiments were performed: 1) in vivo, using the micropuncture technique for the measurements ofJ v; 2) in vitro, using isolated brush border membrane vesicles for the direct measurement of Na+ permeability. In micropuncture studies a 65% increase inJ v of TX rats was observed after treatment with T3. Luminal perfusion of proximal tubules of TX rats with Amphotericin B (10 μg/ml), to increase luminal sodium permeability, enhancedJ v only by 15%. Brush border membrane vesicles isolated from TX and TX+T3 rats showed the same sodium permeability in uptake or efflux experiments. These results were confirmed by the fact that sodium gradient dependent histidine transport into brush border membrane vesicles did not change after T3 treatment. Finally, measuring the amiloride sensitive sodium uptake, it was also found that the Na+−H+ exchange was also only slightly affected by T3. These micropuncture and vesicle data indicate that the large effect of T3 on the trans-cellular sodium transport and volume reabsorption in the proximal tubule, cannot be explained by an action of T3 on the sodium entry step across the brush border membrane. |
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Abstract In order to investigate whether changes in luminal membrane sodium permeability can explain the increase in isotonic fluid reabsorption (J v) found in proximal tubules of thyroidectomized rats (TX) treated with tri-iodothyronine (T3), experiments were carried out on TX rats and TX rats treated for 3 days (TX+T3) with physiological doses (10 μg/kg body wt) of T3. Two sets of experiments were performed: 1) in vivo, using the micropuncture technique for the measurements ofJ v; 2) in vitro, using isolated brush border membrane vesicles for the direct measurement of Na+ permeability. In micropuncture studies a 65% increase inJ v of TX rats was observed after treatment with T3. Luminal perfusion of proximal tubules of TX rats with Amphotericin B (10 μg/ml), to increase luminal sodium permeability, enhancedJ v only by 15%. Brush border membrane vesicles isolated from TX and TX+T3 rats showed the same sodium permeability in uptake or efflux experiments. These results were confirmed by the fact that sodium gradient dependent histidine transport into brush border membrane vesicles did not change after T3 treatment. Finally, measuring the amiloride sensitive sodium uptake, it was also found that the Na+−H+ exchange was also only slightly affected by T3. These micropuncture and vesicle data indicate that the large effect of T3 on the trans-cellular sodium transport and volume reabsorption in the proximal tubule, cannot be explained by an action of T3 on the sodium entry step across the brush border membrane. |
| abstract_unstemmed |
Abstract In order to investigate whether changes in luminal membrane sodium permeability can explain the increase in isotonic fluid reabsorption (J v) found in proximal tubules of thyroidectomized rats (TX) treated with tri-iodothyronine (T3), experiments were carried out on TX rats and TX rats treated for 3 days (TX+T3) with physiological doses (10 μg/kg body wt) of T3. Two sets of experiments were performed: 1) in vivo, using the micropuncture technique for the measurements ofJ v; 2) in vitro, using isolated brush border membrane vesicles for the direct measurement of Na+ permeability. In micropuncture studies a 65% increase inJ v of TX rats was observed after treatment with T3. Luminal perfusion of proximal tubules of TX rats with Amphotericin B (10 μg/ml), to increase luminal sodium permeability, enhancedJ v only by 15%. Brush border membrane vesicles isolated from TX and TX+T3 rats showed the same sodium permeability in uptake or efflux experiments. These results were confirmed by the fact that sodium gradient dependent histidine transport into brush border membrane vesicles did not change after T3 treatment. Finally, measuring the amiloride sensitive sodium uptake, it was also found that the Na+−H+ exchange was also only slightly affected by T3. These micropuncture and vesicle data indicate that the large effect of T3 on the trans-cellular sodium transport and volume reabsorption in the proximal tubule, cannot be explained by an action of T3 on the sodium entry step across the brush border membrane. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ200978195</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210706064135.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070527s1985 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ200978195</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Regulation of volume reabsorption by thyroid hormones in the proximal tubule of rat: Minor role of luminal sodium permeability</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1985</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">8</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract In order to investigate whether changes in luminal membrane sodium permeability can explain the increase in isotonic fluid reabsorption (J v) found in proximal tubules of thyroidectomized rats (TX) treated with tri-iodothyronine (T3), experiments were carried out on TX rats and TX rats treated for 3 days (TX+T3) with physiological doses (10 μg/kg body wt) of T3. Two sets of experiments were performed: 1) in vivo, using the micropuncture technique for the measurements ofJ v; 2) in vitro, using isolated brush border membrane vesicles for the direct measurement of Na+ permeability. In micropuncture studies a 65% increase inJ v of TX rats was observed after treatment with T3. Luminal perfusion of proximal tubules of TX rats with Amphotericin B (10 μg/ml), to increase luminal sodium permeability, enhancedJ v only by 15%. Brush border membrane vesicles isolated from TX and TX+T3 rats showed the same sodium permeability in uptake or efflux experiments. These results were confirmed by the fact that sodium gradient dependent histidine transport into brush border membrane vesicles did not change after T3 treatment. Finally, measuring the amiloride sensitive sodium uptake, it was also found that the Na+−H+ exchange was also only slightly affected by T3. These micropuncture and vesicle data indicate that the large effect of T3 on the trans-cellular sodium transport and volume reabsorption in the proximal tubule, cannot be explained by an action of T3 on the sodium entry step across the brush border membrane.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Springer Online Journal Archives 1860-2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Capasso, Giovambattista</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Kinne-Saffran, Eva</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Santo, Natale G.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Kinne, Rolf</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">Pflügers Archiv</subfield><subfield code="d">1868</subfield><subfield code="g">403(1985) vom: Jan., Seite 97-104</subfield><subfield code="w">(DE-627)NLEJ188987363</subfield><subfield code="w">(DE-600)1463014-x</subfield><subfield code="x">1432-2013</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:403</subfield><subfield code="g">year:1985</subfield><subfield code="g">month:01</subfield><subfield code="g">pages:97-104</subfield><subfield code="g">extent:8</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1007/BF00583288</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SOJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">403</subfield><subfield code="j">1985</subfield><subfield code="c">1</subfield><subfield code="h">97-104</subfield><subfield code="g">8</subfield></datafield></record></collection>
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7.39985 |