K+ and Cl− currents in freshly isolated rat osteoclasts
Abstract Membrane electrical properties of freshly isolated rat osteoclasts were studied using patch-clamp recording methods. Characterization of the passive membrane properties indicated that the osteoclast cell membrane behaved as an isopotential surface. The specific membrane capacitance was 1.2±...
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1991 |
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| Umfang: |
13 |
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| Reproduktion: |
Springer Online Journal Archives 1860-2002 |
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| Übergeordnetes Werk: |
in: Pflügers Archiv - 1868, 419(1991) vom: März/Apr., Seite 358-370 |
| Übergeordnetes Werk: |
volume:419 ; year:1991 ; month:03/04 ; pages:358-370 ; extent:13 |
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NLEJ20099512X |
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| 520 | |a Abstract Membrane electrical properties of freshly isolated rat osteoclasts were studied using patch-clamp recording methods. Characterization of the passive membrane properties indicated that the osteoclast cell membrane behaved as an isopotential surface. The specific membrane capacitance was 1.2±0.3 μF/cm2 (mean ±SD), with no difference between cells plated on glass and those adhering to a permeable collagen substrate. The current/voltage (I/V) relationship of all cells showed inward rectification and I/V curves shifted 51 mV positive per tenfold increase of [K+]out, indicating an inwardly rectifying K+ conductance. The voltage dependence of the K+ chord conductance (g K) also shifted positive along the voltage axis, and the maximum conductance increased, with elevation of [K+]out. g K for cells bathed in 4.7 mM [K+]out increased e-fold per 12mV hyperpolarization, and half-maximal activation was at −89 mV. Approximately 18% (50 pS/pF) of the maximum g K was active at −70 mV. Inward single-channel currents were recorded in cell-attached patches at hyperpolarizing potentials. With symmetrical K+, channel conductance was 25±3 pS and reversal was close to the K+ equilibrium potential, consistent with this K+ channel underlying the whole-cell K+ currents. With both conventional whole-cell and perforated-patch recording, no voltage-activated Ca2+ current was detected. In approximately 30% of osteoclasts studied, an outwardly rectifying current was observed, which was reversibly blocked by 4,4′-diisothiocyanostilbene-2,2′-disulphonic acid (DIDS) and 4-acetamido-4′-isothiocyanostilbene2,2′-disulphonic acid (SITS). This DIDS- and SITS-sensitive current reversed direction at the chloride equilibrium potential. We conclude that an inwardly rectifying K+ current is present in all rat osteoclasts and that some osteoclasts also exhibit an outwardly rectifying Cl− current. Both these membrane conductances may play an important physiological role by dissipating the potential that arises from the electrogenic transport of H+ across the ruffled membrane of the osteoclast. | ||
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(DE-627)NLEJ20099512X DE-627 ger DE-627 rakwb eng K+ and Cl− currents in freshly isolated rat osteoclasts 1991 13 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract Membrane electrical properties of freshly isolated rat osteoclasts were studied using patch-clamp recording methods. Characterization of the passive membrane properties indicated that the osteoclast cell membrane behaved as an isopotential surface. The specific membrane capacitance was 1.2±0.3 μF/cm2 (mean ±SD), with no difference between cells plated on glass and those adhering to a permeable collagen substrate. The current/voltage (I/V) relationship of all cells showed inward rectification and I/V curves shifted 51 mV positive per tenfold increase of [K+]out, indicating an inwardly rectifying K+ conductance. The voltage dependence of the K+ chord conductance (g K) also shifted positive along the voltage axis, and the maximum conductance increased, with elevation of [K+]out. g K for cells bathed in 4.7 mM [K+]out increased e-fold per 12mV hyperpolarization, and half-maximal activation was at −89 mV. Approximately 18% (50 pS/pF) of the maximum g K was active at −70 mV. Inward single-channel currents were recorded in cell-attached patches at hyperpolarizing potentials. With symmetrical K+, channel conductance was 25±3 pS and reversal was close to the K+ equilibrium potential, consistent with this K+ channel underlying the whole-cell K+ currents. With both conventional whole-cell and perforated-patch recording, no voltage-activated Ca2+ current was detected. In approximately 30% of osteoclasts studied, an outwardly rectifying current was observed, which was reversibly blocked by 4,4′-diisothiocyanostilbene-2,2′-disulphonic acid (DIDS) and 4-acetamido-4′-isothiocyanostilbene2,2′-disulphonic acid (SITS). This DIDS- and SITS-sensitive current reversed direction at the chloride equilibrium potential. We conclude that an inwardly rectifying K+ current is present in all rat osteoclasts and that some osteoclasts also exhibit an outwardly rectifying Cl− current. Both these membrane conductances may play an important physiological role by dissipating the potential that arises from the electrogenic transport of H+ across the ruffled membrane of the osteoclast. Springer Online Journal Archives 1860-2002 Sims, Stephen M. oth Kelly, Melanie E. M. oth Dixon, S. Jeffrey oth in Pflügers Archiv 1868 419(1991) vom: März/Apr., Seite 358-370 (DE-627)NLEJ188987363 (DE-600)1463014-x 1432-2013 nnns volume:419 year:1991 month:03/04 pages:358-370 extent:13 http://dx.doi.org/10.1007/BF00371118 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 419 1991 3/4 358-370 13 |
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(DE-627)NLEJ20099512X DE-627 ger DE-627 rakwb eng K+ and Cl− currents in freshly isolated rat osteoclasts 1991 13 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract Membrane electrical properties of freshly isolated rat osteoclasts were studied using patch-clamp recording methods. Characterization of the passive membrane properties indicated that the osteoclast cell membrane behaved as an isopotential surface. The specific membrane capacitance was 1.2±0.3 μF/cm2 (mean ±SD), with no difference between cells plated on glass and those adhering to a permeable collagen substrate. The current/voltage (I/V) relationship of all cells showed inward rectification and I/V curves shifted 51 mV positive per tenfold increase of [K+]out, indicating an inwardly rectifying K+ conductance. The voltage dependence of the K+ chord conductance (g K) also shifted positive along the voltage axis, and the maximum conductance increased, with elevation of [K+]out. g K for cells bathed in 4.7 mM [K+]out increased e-fold per 12mV hyperpolarization, and half-maximal activation was at −89 mV. Approximately 18% (50 pS/pF) of the maximum g K was active at −70 mV. Inward single-channel currents were recorded in cell-attached patches at hyperpolarizing potentials. With symmetrical K+, channel conductance was 25±3 pS and reversal was close to the K+ equilibrium potential, consistent with this K+ channel underlying the whole-cell K+ currents. With both conventional whole-cell and perforated-patch recording, no voltage-activated Ca2+ current was detected. In approximately 30% of osteoclasts studied, an outwardly rectifying current was observed, which was reversibly blocked by 4,4′-diisothiocyanostilbene-2,2′-disulphonic acid (DIDS) and 4-acetamido-4′-isothiocyanostilbene2,2′-disulphonic acid (SITS). This DIDS- and SITS-sensitive current reversed direction at the chloride equilibrium potential. We conclude that an inwardly rectifying K+ current is present in all rat osteoclasts and that some osteoclasts also exhibit an outwardly rectifying Cl− current. Both these membrane conductances may play an important physiological role by dissipating the potential that arises from the electrogenic transport of H+ across the ruffled membrane of the osteoclast. Springer Online Journal Archives 1860-2002 Sims, Stephen M. oth Kelly, Melanie E. M. oth Dixon, S. Jeffrey oth in Pflügers Archiv 1868 419(1991) vom: März/Apr., Seite 358-370 (DE-627)NLEJ188987363 (DE-600)1463014-x 1432-2013 nnns volume:419 year:1991 month:03/04 pages:358-370 extent:13 http://dx.doi.org/10.1007/BF00371118 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 419 1991 3/4 358-370 13 |
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(DE-627)NLEJ20099512X DE-627 ger DE-627 rakwb eng K+ and Cl− currents in freshly isolated rat osteoclasts 1991 13 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract Membrane electrical properties of freshly isolated rat osteoclasts were studied using patch-clamp recording methods. Characterization of the passive membrane properties indicated that the osteoclast cell membrane behaved as an isopotential surface. The specific membrane capacitance was 1.2±0.3 μF/cm2 (mean ±SD), with no difference between cells plated on glass and those adhering to a permeable collagen substrate. The current/voltage (I/V) relationship of all cells showed inward rectification and I/V curves shifted 51 mV positive per tenfold increase of [K+]out, indicating an inwardly rectifying K+ conductance. The voltage dependence of the K+ chord conductance (g K) also shifted positive along the voltage axis, and the maximum conductance increased, with elevation of [K+]out. g K for cells bathed in 4.7 mM [K+]out increased e-fold per 12mV hyperpolarization, and half-maximal activation was at −89 mV. Approximately 18% (50 pS/pF) of the maximum g K was active at −70 mV. Inward single-channel currents were recorded in cell-attached patches at hyperpolarizing potentials. With symmetrical K+, channel conductance was 25±3 pS and reversal was close to the K+ equilibrium potential, consistent with this K+ channel underlying the whole-cell K+ currents. With both conventional whole-cell and perforated-patch recording, no voltage-activated Ca2+ current was detected. In approximately 30% of osteoclasts studied, an outwardly rectifying current was observed, which was reversibly blocked by 4,4′-diisothiocyanostilbene-2,2′-disulphonic acid (DIDS) and 4-acetamido-4′-isothiocyanostilbene2,2′-disulphonic acid (SITS). This DIDS- and SITS-sensitive current reversed direction at the chloride equilibrium potential. We conclude that an inwardly rectifying K+ current is present in all rat osteoclasts and that some osteoclasts also exhibit an outwardly rectifying Cl− current. Both these membrane conductances may play an important physiological role by dissipating the potential that arises from the electrogenic transport of H+ across the ruffled membrane of the osteoclast. Springer Online Journal Archives 1860-2002 Sims, Stephen M. oth Kelly, Melanie E. M. oth Dixon, S. Jeffrey oth in Pflügers Archiv 1868 419(1991) vom: März/Apr., Seite 358-370 (DE-627)NLEJ188987363 (DE-600)1463014-x 1432-2013 nnns volume:419 year:1991 month:03/04 pages:358-370 extent:13 http://dx.doi.org/10.1007/BF00371118 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 419 1991 3/4 358-370 13 |
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(DE-627)NLEJ20099512X DE-627 ger DE-627 rakwb eng K+ and Cl− currents in freshly isolated rat osteoclasts 1991 13 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract Membrane electrical properties of freshly isolated rat osteoclasts were studied using patch-clamp recording methods. Characterization of the passive membrane properties indicated that the osteoclast cell membrane behaved as an isopotential surface. The specific membrane capacitance was 1.2±0.3 μF/cm2 (mean ±SD), with no difference between cells plated on glass and those adhering to a permeable collagen substrate. The current/voltage (I/V) relationship of all cells showed inward rectification and I/V curves shifted 51 mV positive per tenfold increase of [K+]out, indicating an inwardly rectifying K+ conductance. The voltage dependence of the K+ chord conductance (g K) also shifted positive along the voltage axis, and the maximum conductance increased, with elevation of [K+]out. g K for cells bathed in 4.7 mM [K+]out increased e-fold per 12mV hyperpolarization, and half-maximal activation was at −89 mV. Approximately 18% (50 pS/pF) of the maximum g K was active at −70 mV. Inward single-channel currents were recorded in cell-attached patches at hyperpolarizing potentials. With symmetrical K+, channel conductance was 25±3 pS and reversal was close to the K+ equilibrium potential, consistent with this K+ channel underlying the whole-cell K+ currents. With both conventional whole-cell and perforated-patch recording, no voltage-activated Ca2+ current was detected. In approximately 30% of osteoclasts studied, an outwardly rectifying current was observed, which was reversibly blocked by 4,4′-diisothiocyanostilbene-2,2′-disulphonic acid (DIDS) and 4-acetamido-4′-isothiocyanostilbene2,2′-disulphonic acid (SITS). This DIDS- and SITS-sensitive current reversed direction at the chloride equilibrium potential. We conclude that an inwardly rectifying K+ current is present in all rat osteoclasts and that some osteoclasts also exhibit an outwardly rectifying Cl− current. Both these membrane conductances may play an important physiological role by dissipating the potential that arises from the electrogenic transport of H+ across the ruffled membrane of the osteoclast. Springer Online Journal Archives 1860-2002 Sims, Stephen M. oth Kelly, Melanie E. M. oth Dixon, S. Jeffrey oth in Pflügers Archiv 1868 419(1991) vom: März/Apr., Seite 358-370 (DE-627)NLEJ188987363 (DE-600)1463014-x 1432-2013 nnns volume:419 year:1991 month:03/04 pages:358-370 extent:13 http://dx.doi.org/10.1007/BF00371118 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 419 1991 3/4 358-370 13 |
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(DE-627)NLEJ20099512X DE-627 ger DE-627 rakwb eng K+ and Cl− currents in freshly isolated rat osteoclasts 1991 13 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract Membrane electrical properties of freshly isolated rat osteoclasts were studied using patch-clamp recording methods. Characterization of the passive membrane properties indicated that the osteoclast cell membrane behaved as an isopotential surface. The specific membrane capacitance was 1.2±0.3 μF/cm2 (mean ±SD), with no difference between cells plated on glass and those adhering to a permeable collagen substrate. The current/voltage (I/V) relationship of all cells showed inward rectification and I/V curves shifted 51 mV positive per tenfold increase of [K+]out, indicating an inwardly rectifying K+ conductance. The voltage dependence of the K+ chord conductance (g K) also shifted positive along the voltage axis, and the maximum conductance increased, with elevation of [K+]out. g K for cells bathed in 4.7 mM [K+]out increased e-fold per 12mV hyperpolarization, and half-maximal activation was at −89 mV. Approximately 18% (50 pS/pF) of the maximum g K was active at −70 mV. Inward single-channel currents were recorded in cell-attached patches at hyperpolarizing potentials. With symmetrical K+, channel conductance was 25±3 pS and reversal was close to the K+ equilibrium potential, consistent with this K+ channel underlying the whole-cell K+ currents. With both conventional whole-cell and perforated-patch recording, no voltage-activated Ca2+ current was detected. In approximately 30% of osteoclasts studied, an outwardly rectifying current was observed, which was reversibly blocked by 4,4′-diisothiocyanostilbene-2,2′-disulphonic acid (DIDS) and 4-acetamido-4′-isothiocyanostilbene2,2′-disulphonic acid (SITS). This DIDS- and SITS-sensitive current reversed direction at the chloride equilibrium potential. We conclude that an inwardly rectifying K+ current is present in all rat osteoclasts and that some osteoclasts also exhibit an outwardly rectifying Cl− current. Both these membrane conductances may play an important physiological role by dissipating the potential that arises from the electrogenic transport of H+ across the ruffled membrane of the osteoclast. Springer Online Journal Archives 1860-2002 Sims, Stephen M. oth Kelly, Melanie E. M. oth Dixon, S. Jeffrey oth in Pflügers Archiv 1868 419(1991) vom: März/Apr., Seite 358-370 (DE-627)NLEJ188987363 (DE-600)1463014-x 1432-2013 nnns volume:419 year:1991 month:03/04 pages:358-370 extent:13 http://dx.doi.org/10.1007/BF00371118 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 419 1991 3/4 358-370 13 |
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k+ and cl− currents in freshly isolated rat osteoclasts |
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K+ and Cl− currents in freshly isolated rat osteoclasts |
| abstract |
Abstract Membrane electrical properties of freshly isolated rat osteoclasts were studied using patch-clamp recording methods. Characterization of the passive membrane properties indicated that the osteoclast cell membrane behaved as an isopotential surface. The specific membrane capacitance was 1.2±0.3 μF/cm2 (mean ±SD), with no difference between cells plated on glass and those adhering to a permeable collagen substrate. The current/voltage (I/V) relationship of all cells showed inward rectification and I/V curves shifted 51 mV positive per tenfold increase of [K+]out, indicating an inwardly rectifying K+ conductance. The voltage dependence of the K+ chord conductance (g K) also shifted positive along the voltage axis, and the maximum conductance increased, with elevation of [K+]out. g K for cells bathed in 4.7 mM [K+]out increased e-fold per 12mV hyperpolarization, and half-maximal activation was at −89 mV. Approximately 18% (50 pS/pF) of the maximum g K was active at −70 mV. Inward single-channel currents were recorded in cell-attached patches at hyperpolarizing potentials. With symmetrical K+, channel conductance was 25±3 pS and reversal was close to the K+ equilibrium potential, consistent with this K+ channel underlying the whole-cell K+ currents. With both conventional whole-cell and perforated-patch recording, no voltage-activated Ca2+ current was detected. In approximately 30% of osteoclasts studied, an outwardly rectifying current was observed, which was reversibly blocked by 4,4′-diisothiocyanostilbene-2,2′-disulphonic acid (DIDS) and 4-acetamido-4′-isothiocyanostilbene2,2′-disulphonic acid (SITS). This DIDS- and SITS-sensitive current reversed direction at the chloride equilibrium potential. We conclude that an inwardly rectifying K+ current is present in all rat osteoclasts and that some osteoclasts also exhibit an outwardly rectifying Cl− current. Both these membrane conductances may play an important physiological role by dissipating the potential that arises from the electrogenic transport of H+ across the ruffled membrane of the osteoclast. |
| abstractGer |
Abstract Membrane electrical properties of freshly isolated rat osteoclasts were studied using patch-clamp recording methods. Characterization of the passive membrane properties indicated that the osteoclast cell membrane behaved as an isopotential surface. The specific membrane capacitance was 1.2±0.3 μF/cm2 (mean ±SD), with no difference between cells plated on glass and those adhering to a permeable collagen substrate. The current/voltage (I/V) relationship of all cells showed inward rectification and I/V curves shifted 51 mV positive per tenfold increase of [K+]out, indicating an inwardly rectifying K+ conductance. The voltage dependence of the K+ chord conductance (g K) also shifted positive along the voltage axis, and the maximum conductance increased, with elevation of [K+]out. g K for cells bathed in 4.7 mM [K+]out increased e-fold per 12mV hyperpolarization, and half-maximal activation was at −89 mV. Approximately 18% (50 pS/pF) of the maximum g K was active at −70 mV. Inward single-channel currents were recorded in cell-attached patches at hyperpolarizing potentials. With symmetrical K+, channel conductance was 25±3 pS and reversal was close to the K+ equilibrium potential, consistent with this K+ channel underlying the whole-cell K+ currents. With both conventional whole-cell and perforated-patch recording, no voltage-activated Ca2+ current was detected. In approximately 30% of osteoclasts studied, an outwardly rectifying current was observed, which was reversibly blocked by 4,4′-diisothiocyanostilbene-2,2′-disulphonic acid (DIDS) and 4-acetamido-4′-isothiocyanostilbene2,2′-disulphonic acid (SITS). This DIDS- and SITS-sensitive current reversed direction at the chloride equilibrium potential. We conclude that an inwardly rectifying K+ current is present in all rat osteoclasts and that some osteoclasts also exhibit an outwardly rectifying Cl− current. Both these membrane conductances may play an important physiological role by dissipating the potential that arises from the electrogenic transport of H+ across the ruffled membrane of the osteoclast. |
| abstract_unstemmed |
Abstract Membrane electrical properties of freshly isolated rat osteoclasts were studied using patch-clamp recording methods. Characterization of the passive membrane properties indicated that the osteoclast cell membrane behaved as an isopotential surface. The specific membrane capacitance was 1.2±0.3 μF/cm2 (mean ±SD), with no difference between cells plated on glass and those adhering to a permeable collagen substrate. The current/voltage (I/V) relationship of all cells showed inward rectification and I/V curves shifted 51 mV positive per tenfold increase of [K+]out, indicating an inwardly rectifying K+ conductance. The voltage dependence of the K+ chord conductance (g K) also shifted positive along the voltage axis, and the maximum conductance increased, with elevation of [K+]out. g K for cells bathed in 4.7 mM [K+]out increased e-fold per 12mV hyperpolarization, and half-maximal activation was at −89 mV. Approximately 18% (50 pS/pF) of the maximum g K was active at −70 mV. Inward single-channel currents were recorded in cell-attached patches at hyperpolarizing potentials. With symmetrical K+, channel conductance was 25±3 pS and reversal was close to the K+ equilibrium potential, consistent with this K+ channel underlying the whole-cell K+ currents. With both conventional whole-cell and perforated-patch recording, no voltage-activated Ca2+ current was detected. In approximately 30% of osteoclasts studied, an outwardly rectifying current was observed, which was reversibly blocked by 4,4′-diisothiocyanostilbene-2,2′-disulphonic acid (DIDS) and 4-acetamido-4′-isothiocyanostilbene2,2′-disulphonic acid (SITS). This DIDS- and SITS-sensitive current reversed direction at the chloride equilibrium potential. We conclude that an inwardly rectifying K+ current is present in all rat osteoclasts and that some osteoclasts also exhibit an outwardly rectifying Cl− current. Both these membrane conductances may play an important physiological role by dissipating the potential that arises from the electrogenic transport of H+ across the ruffled membrane of the osteoclast. |
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GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE |
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K+ and Cl− currents in freshly isolated rat osteoclasts |
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