Effect of parathyroid hormone on acid/base transport in rabbit renal proximal tubule S3 segment
Abstract The effect of parathyroid hormone (PTH) on acid/base transport in isolated rabbit renal proximal tubule S3 segment was investigated with double-barreled and conventional microelectrodes. PTH (10 nM) induced a small depolarization and enhanced the initial rates of cell pH (pHi) increase and...
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E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1993 |
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7 |
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Springer Online Journal Archives 1860-2002 |
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| Übergeordnetes Werk: |
in: Pflügers Archiv - 1868, 423(1993) vom: Jan./Feb., Seite 7-13 |
| Übergeordnetes Werk: |
volume:423 ; year:1993 ; month:01/02 ; pages:7-13 ; extent:7 |
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| 245 | 1 | 0 | |a Effect of parathyroid hormone on acid/base transport in rabbit renal proximal tubule S3 segment |
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| 520 | |a Abstract The effect of parathyroid hormone (PTH) on acid/base transport in isolated rabbit renal proximal tubule S3 segment was investigated with double-barreled and conventional microelectrodes. PTH (10 nM) induced a small depolarization and enhanced the initial rates of cell pH (pHi) increase and cell Cl− ([Cl−]i) decrease in response to bath Cl− removal by 28.0±2.1% and 31.0±6.4% respectively. The calculated initial HCO3 − influx to bath Cl− removal was also enhanced by 28%. On the other hand, PTH reduced the initial rate of pHi decrease to luminal Na+ removal in the absence of HCO3 −/CO2 by 20.4±3.9%. The PTH-induced depolarization was not accompanied with changes in steadystate pHi or [Cl−]i levels, but was greatly attenuated in the presence of ouabain (0.1 mM). Either dibutyrylcAMP (0.1 mM) plus theophylline (1 mM) or forskolin (10 μM) alone could reproduce all the effects of PTH. These results indicate that (a) PTH inhibits the luminal Na+/H+ exchanger but stimulates the basolateral Cl−/HCO3 − exchanger in the S3 segment; (b) the PTH-induced depolarization largely results from inhibition of Na+/K+-ATPase and (c) all these effects are at least partly mediated by a cAMP-dependent mechanism. | ||
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(DE-627)NLEJ20099901X DE-627 ger DE-627 rakwb eng Effect of parathyroid hormone on acid/base transport in rabbit renal proximal tubule S3 segment 1993 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract The effect of parathyroid hormone (PTH) on acid/base transport in isolated rabbit renal proximal tubule S3 segment was investigated with double-barreled and conventional microelectrodes. PTH (10 nM) induced a small depolarization and enhanced the initial rates of cell pH (pHi) increase and cell Cl− ([Cl−]i) decrease in response to bath Cl− removal by 28.0±2.1% and 31.0±6.4% respectively. The calculated initial HCO3 − influx to bath Cl− removal was also enhanced by 28%. On the other hand, PTH reduced the initial rate of pHi decrease to luminal Na+ removal in the absence of HCO3 −/CO2 by 20.4±3.9%. The PTH-induced depolarization was not accompanied with changes in steadystate pHi or [Cl−]i levels, but was greatly attenuated in the presence of ouabain (0.1 mM). Either dibutyrylcAMP (0.1 mM) plus theophylline (1 mM) or forskolin (10 μM) alone could reproduce all the effects of PTH. These results indicate that (a) PTH inhibits the luminal Na+/H+ exchanger but stimulates the basolateral Cl−/HCO3 − exchanger in the S3 segment; (b) the PTH-induced depolarization largely results from inhibition of Na+/K+-ATPase and (c) all these effects are at least partly mediated by a cAMP-dependent mechanism. Springer Online Journal Archives 1860-2002 Seki, George oth Taniguchi, Shigeo oth Uwatoko, Shu oth Suzuki, Keiji oth Kurokawa, Kiyoshi oth in Pflügers Archiv 1868 423(1993) vom: Jan./Feb., Seite 7-13 (DE-627)NLEJ188987363 (DE-600)1463014-x 1432-2013 nnns volume:423 year:1993 month:01/02 pages:7-13 extent:7 http://dx.doi.org/10.1007/BF00374954 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 423 1993 1/2 7-13 7 |
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(DE-627)NLEJ20099901X DE-627 ger DE-627 rakwb eng Effect of parathyroid hormone on acid/base transport in rabbit renal proximal tubule S3 segment 1993 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract The effect of parathyroid hormone (PTH) on acid/base transport in isolated rabbit renal proximal tubule S3 segment was investigated with double-barreled and conventional microelectrodes. PTH (10 nM) induced a small depolarization and enhanced the initial rates of cell pH (pHi) increase and cell Cl− ([Cl−]i) decrease in response to bath Cl− removal by 28.0±2.1% and 31.0±6.4% respectively. The calculated initial HCO3 − influx to bath Cl− removal was also enhanced by 28%. On the other hand, PTH reduced the initial rate of pHi decrease to luminal Na+ removal in the absence of HCO3 −/CO2 by 20.4±3.9%. The PTH-induced depolarization was not accompanied with changes in steadystate pHi or [Cl−]i levels, but was greatly attenuated in the presence of ouabain (0.1 mM). Either dibutyrylcAMP (0.1 mM) plus theophylline (1 mM) or forskolin (10 μM) alone could reproduce all the effects of PTH. These results indicate that (a) PTH inhibits the luminal Na+/H+ exchanger but stimulates the basolateral Cl−/HCO3 − exchanger in the S3 segment; (b) the PTH-induced depolarization largely results from inhibition of Na+/K+-ATPase and (c) all these effects are at least partly mediated by a cAMP-dependent mechanism. Springer Online Journal Archives 1860-2002 Seki, George oth Taniguchi, Shigeo oth Uwatoko, Shu oth Suzuki, Keiji oth Kurokawa, Kiyoshi oth in Pflügers Archiv 1868 423(1993) vom: Jan./Feb., Seite 7-13 (DE-627)NLEJ188987363 (DE-600)1463014-x 1432-2013 nnns volume:423 year:1993 month:01/02 pages:7-13 extent:7 http://dx.doi.org/10.1007/BF00374954 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 423 1993 1/2 7-13 7 |
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(DE-627)NLEJ20099901X DE-627 ger DE-627 rakwb eng Effect of parathyroid hormone on acid/base transport in rabbit renal proximal tubule S3 segment 1993 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract The effect of parathyroid hormone (PTH) on acid/base transport in isolated rabbit renal proximal tubule S3 segment was investigated with double-barreled and conventional microelectrodes. PTH (10 nM) induced a small depolarization and enhanced the initial rates of cell pH (pHi) increase and cell Cl− ([Cl−]i) decrease in response to bath Cl− removal by 28.0±2.1% and 31.0±6.4% respectively. The calculated initial HCO3 − influx to bath Cl− removal was also enhanced by 28%. On the other hand, PTH reduced the initial rate of pHi decrease to luminal Na+ removal in the absence of HCO3 −/CO2 by 20.4±3.9%. The PTH-induced depolarization was not accompanied with changes in steadystate pHi or [Cl−]i levels, but was greatly attenuated in the presence of ouabain (0.1 mM). Either dibutyrylcAMP (0.1 mM) plus theophylline (1 mM) or forskolin (10 μM) alone could reproduce all the effects of PTH. These results indicate that (a) PTH inhibits the luminal Na+/H+ exchanger but stimulates the basolateral Cl−/HCO3 − exchanger in the S3 segment; (b) the PTH-induced depolarization largely results from inhibition of Na+/K+-ATPase and (c) all these effects are at least partly mediated by a cAMP-dependent mechanism. Springer Online Journal Archives 1860-2002 Seki, George oth Taniguchi, Shigeo oth Uwatoko, Shu oth Suzuki, Keiji oth Kurokawa, Kiyoshi oth in Pflügers Archiv 1868 423(1993) vom: Jan./Feb., Seite 7-13 (DE-627)NLEJ188987363 (DE-600)1463014-x 1432-2013 nnns volume:423 year:1993 month:01/02 pages:7-13 extent:7 http://dx.doi.org/10.1007/BF00374954 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 423 1993 1/2 7-13 7 |
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(DE-627)NLEJ20099901X DE-627 ger DE-627 rakwb eng Effect of parathyroid hormone on acid/base transport in rabbit renal proximal tubule S3 segment 1993 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract The effect of parathyroid hormone (PTH) on acid/base transport in isolated rabbit renal proximal tubule S3 segment was investigated with double-barreled and conventional microelectrodes. PTH (10 nM) induced a small depolarization and enhanced the initial rates of cell pH (pHi) increase and cell Cl− ([Cl−]i) decrease in response to bath Cl− removal by 28.0±2.1% and 31.0±6.4% respectively. The calculated initial HCO3 − influx to bath Cl− removal was also enhanced by 28%. On the other hand, PTH reduced the initial rate of pHi decrease to luminal Na+ removal in the absence of HCO3 −/CO2 by 20.4±3.9%. The PTH-induced depolarization was not accompanied with changes in steadystate pHi or [Cl−]i levels, but was greatly attenuated in the presence of ouabain (0.1 mM). Either dibutyrylcAMP (0.1 mM) plus theophylline (1 mM) or forskolin (10 μM) alone could reproduce all the effects of PTH. These results indicate that (a) PTH inhibits the luminal Na+/H+ exchanger but stimulates the basolateral Cl−/HCO3 − exchanger in the S3 segment; (b) the PTH-induced depolarization largely results from inhibition of Na+/K+-ATPase and (c) all these effects are at least partly mediated by a cAMP-dependent mechanism. Springer Online Journal Archives 1860-2002 Seki, George oth Taniguchi, Shigeo oth Uwatoko, Shu oth Suzuki, Keiji oth Kurokawa, Kiyoshi oth in Pflügers Archiv 1868 423(1993) vom: Jan./Feb., Seite 7-13 (DE-627)NLEJ188987363 (DE-600)1463014-x 1432-2013 nnns volume:423 year:1993 month:01/02 pages:7-13 extent:7 http://dx.doi.org/10.1007/BF00374954 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 423 1993 1/2 7-13 7 |
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(DE-627)NLEJ20099901X DE-627 ger DE-627 rakwb eng Effect of parathyroid hormone on acid/base transport in rabbit renal proximal tubule S3 segment 1993 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract The effect of parathyroid hormone (PTH) on acid/base transport in isolated rabbit renal proximal tubule S3 segment was investigated with double-barreled and conventional microelectrodes. PTH (10 nM) induced a small depolarization and enhanced the initial rates of cell pH (pHi) increase and cell Cl− ([Cl−]i) decrease in response to bath Cl− removal by 28.0±2.1% and 31.0±6.4% respectively. The calculated initial HCO3 − influx to bath Cl− removal was also enhanced by 28%. On the other hand, PTH reduced the initial rate of pHi decrease to luminal Na+ removal in the absence of HCO3 −/CO2 by 20.4±3.9%. The PTH-induced depolarization was not accompanied with changes in steadystate pHi or [Cl−]i levels, but was greatly attenuated in the presence of ouabain (0.1 mM). Either dibutyrylcAMP (0.1 mM) plus theophylline (1 mM) or forskolin (10 μM) alone could reproduce all the effects of PTH. These results indicate that (a) PTH inhibits the luminal Na+/H+ exchanger but stimulates the basolateral Cl−/HCO3 − exchanger in the S3 segment; (b) the PTH-induced depolarization largely results from inhibition of Na+/K+-ATPase and (c) all these effects are at least partly mediated by a cAMP-dependent mechanism. Springer Online Journal Archives 1860-2002 Seki, George oth Taniguchi, Shigeo oth Uwatoko, Shu oth Suzuki, Keiji oth Kurokawa, Kiyoshi oth in Pflügers Archiv 1868 423(1993) vom: Jan./Feb., Seite 7-13 (DE-627)NLEJ188987363 (DE-600)1463014-x 1432-2013 nnns volume:423 year:1993 month:01/02 pages:7-13 extent:7 http://dx.doi.org/10.1007/BF00374954 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 423 1993 1/2 7-13 7 |
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Abstract The effect of parathyroid hormone (PTH) on acid/base transport in isolated rabbit renal proximal tubule S3 segment was investigated with double-barreled and conventional microelectrodes. PTH (10 nM) induced a small depolarization and enhanced the initial rates of cell pH (pHi) increase and cell Cl− ([Cl−]i) decrease in response to bath Cl− removal by 28.0±2.1% and 31.0±6.4% respectively. The calculated initial HCO3 − influx to bath Cl− removal was also enhanced by 28%. On the other hand, PTH reduced the initial rate of pHi decrease to luminal Na+ removal in the absence of HCO3 −/CO2 by 20.4±3.9%. The PTH-induced depolarization was not accompanied with changes in steadystate pHi or [Cl−]i levels, but was greatly attenuated in the presence of ouabain (0.1 mM). Either dibutyrylcAMP (0.1 mM) plus theophylline (1 mM) or forskolin (10 μM) alone could reproduce all the effects of PTH. These results indicate that (a) PTH inhibits the luminal Na+/H+ exchanger but stimulates the basolateral Cl−/HCO3 − exchanger in the S3 segment; (b) the PTH-induced depolarization largely results from inhibition of Na+/K+-ATPase and (c) all these effects are at least partly mediated by a cAMP-dependent mechanism. |
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Abstract The effect of parathyroid hormone (PTH) on acid/base transport in isolated rabbit renal proximal tubule S3 segment was investigated with double-barreled and conventional microelectrodes. PTH (10 nM) induced a small depolarization and enhanced the initial rates of cell pH (pHi) increase and cell Cl− ([Cl−]i) decrease in response to bath Cl− removal by 28.0±2.1% and 31.0±6.4% respectively. The calculated initial HCO3 − influx to bath Cl− removal was also enhanced by 28%. On the other hand, PTH reduced the initial rate of pHi decrease to luminal Na+ removal in the absence of HCO3 −/CO2 by 20.4±3.9%. The PTH-induced depolarization was not accompanied with changes in steadystate pHi or [Cl−]i levels, but was greatly attenuated in the presence of ouabain (0.1 mM). Either dibutyrylcAMP (0.1 mM) plus theophylline (1 mM) or forskolin (10 μM) alone could reproduce all the effects of PTH. These results indicate that (a) PTH inhibits the luminal Na+/H+ exchanger but stimulates the basolateral Cl−/HCO3 − exchanger in the S3 segment; (b) the PTH-induced depolarization largely results from inhibition of Na+/K+-ATPase and (c) all these effects are at least partly mediated by a cAMP-dependent mechanism. |
| abstract_unstemmed |
Abstract The effect of parathyroid hormone (PTH) on acid/base transport in isolated rabbit renal proximal tubule S3 segment was investigated with double-barreled and conventional microelectrodes. PTH (10 nM) induced a small depolarization and enhanced the initial rates of cell pH (pHi) increase and cell Cl− ([Cl−]i) decrease in response to bath Cl− removal by 28.0±2.1% and 31.0±6.4% respectively. The calculated initial HCO3 − influx to bath Cl− removal was also enhanced by 28%. On the other hand, PTH reduced the initial rate of pHi decrease to luminal Na+ removal in the absence of HCO3 −/CO2 by 20.4±3.9%. The PTH-induced depolarization was not accompanied with changes in steadystate pHi or [Cl−]i levels, but was greatly attenuated in the presence of ouabain (0.1 mM). Either dibutyrylcAMP (0.1 mM) plus theophylline (1 mM) or forskolin (10 μM) alone could reproduce all the effects of PTH. These results indicate that (a) PTH inhibits the luminal Na+/H+ exchanger but stimulates the basolateral Cl−/HCO3 − exchanger in the S3 segment; (b) the PTH-induced depolarization largely results from inhibition of Na+/K+-ATPase and (c) all these effects are at least partly mediated by a cAMP-dependent mechanism. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ20099901X</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210706064442.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070527s1993 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ20099901X</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Effect of parathyroid hormone on acid/base transport in rabbit renal proximal tubule S3 segment</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1993</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">7</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract The effect of parathyroid hormone (PTH) on acid/base transport in isolated rabbit renal proximal tubule S3 segment was investigated with double-barreled and conventional microelectrodes. PTH (10 nM) induced a small depolarization and enhanced the initial rates of cell pH (pHi) increase and cell Cl− ([Cl−]i) decrease in response to bath Cl− removal by 28.0±2.1% and 31.0±6.4% respectively. The calculated initial HCO3 − influx to bath Cl− removal was also enhanced by 28%. On the other hand, PTH reduced the initial rate of pHi decrease to luminal Na+ removal in the absence of HCO3 −/CO2 by 20.4±3.9%. The PTH-induced depolarization was not accompanied with changes in steadystate pHi or [Cl−]i levels, but was greatly attenuated in the presence of ouabain (0.1 mM). Either dibutyrylcAMP (0.1 mM) plus theophylline (1 mM) or forskolin (10 μM) alone could reproduce all the effects of PTH. These results indicate that (a) PTH inhibits the luminal Na+/H+ exchanger but stimulates the basolateral Cl−/HCO3 − exchanger in the S3 segment; (b) the PTH-induced depolarization largely results from inhibition of Na+/K+-ATPase and (c) all these effects are at least partly mediated by a cAMP-dependent mechanism.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Springer Online Journal Archives 1860-2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Seki, George</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Taniguchi, Shigeo</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Uwatoko, Shu</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Suzuki, Keiji</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Kurokawa, Kiyoshi</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">Pflügers Archiv</subfield><subfield code="d">1868</subfield><subfield code="g">423(1993) vom: Jan./Feb., Seite 7-13</subfield><subfield code="w">(DE-627)NLEJ188987363</subfield><subfield code="w">(DE-600)1463014-x</subfield><subfield code="x">1432-2013</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:423</subfield><subfield code="g">year:1993</subfield><subfield code="g">month:01/02</subfield><subfield code="g">pages:7-13</subfield><subfield code="g">extent:7</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1007/BF00374954</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SOJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">423</subfield><subfield code="j">1993</subfield><subfield code="c">1/2</subfield><subfield code="h">7-13</subfield><subfield code="g">7</subfield></datafield></record></collection>
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