Asymmetrical labeling of giant phospholipid vesicles
Abstract The method for labeling of inner membrane leaflet in unilamellar giant POPC vesicles was developed and characterised. Symmetrically NBD-PC labeled vesicles were treated by sodium dithionite, which undergoes an irreversible chemical reaction with NBD-PC molecule making it non-fluorescent. Af...
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2000 |
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| Reproduktion: |
Springer Online Journal Archives 1860-2002 |
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| Übergeordnetes Werk: |
in: Pflügers Archiv - 1868, 440(2000) vom: Juli, Seite r051 |
| Übergeordnetes Werk: |
volume:440 ; year:2000 ; month:07 ; pages:r051 |
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NLEJ201021277 |
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| 520 | |a Abstract The method for labeling of inner membrane leaflet in unilamellar giant POPC vesicles was developed and characterised. Symmetrically NBD-PC labeled vesicles were treated by sodium dithionite, which undergoes an irreversible chemical reaction with NBD-PC molecule making it non-fluorescent. After the addition of dithionite the fluorescence on single vesicles as well as on vesicle suspension showed a 50 % decrease of its initial value corresponding to marker quenching in the outer leaflet. Hence, flourimetry as well as flourescence microscopy prove that dithionite quenching is a suitable method to induce an asymmetrical labeling of the NBD-PC marked giant vesicles. | ||
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| 700 | 1 | |a Gomišček, Gregor |4 oth | |
| 700 | 1 | |a Arrigler, Vesna |4 oth | |
| 700 | 1 | |a Gros, Marjan |4 oth | |
| 700 | 1 | |a Zupančič, Mirjana |4 oth | |
| 700 | 1 | |a Svetina, Saša |4 oth | |
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(DE-627)NLEJ201021277 DE-627 ger DE-627 rakwb eng Asymmetrical labeling of giant phospholipid vesicles 2000 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract The method for labeling of inner membrane leaflet in unilamellar giant POPC vesicles was developed and characterised. Symmetrically NBD-PC labeled vesicles were treated by sodium dithionite, which undergoes an irreversible chemical reaction with NBD-PC molecule making it non-fluorescent. After the addition of dithionite the fluorescence on single vesicles as well as on vesicle suspension showed a 50 % decrease of its initial value corresponding to marker quenching in the outer leaflet. Hence, flourimetry as well as flourescence microscopy prove that dithionite quenching is a suitable method to induce an asymmetrical labeling of the NBD-PC marked giant vesicles. Springer Online Journal Archives 1860-2002 Gomišček, Gregor oth Arrigler, Vesna oth Gros, Marjan oth Zupančič, Mirjana oth Svetina, Saša oth in Pflügers Archiv 1868 440(2000) vom: Juli, Seite r051 (DE-627)NLEJ188987363 (DE-600)1463014-x 1432-2013 nnns volume:440 year:2000 month:07 pages:r051 http://dx.doi.org/10.1007/s004240000003 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 440 2000 7 r051 |
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(DE-627)NLEJ201021277 DE-627 ger DE-627 rakwb eng Asymmetrical labeling of giant phospholipid vesicles 2000 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract The method for labeling of inner membrane leaflet in unilamellar giant POPC vesicles was developed and characterised. Symmetrically NBD-PC labeled vesicles were treated by sodium dithionite, which undergoes an irreversible chemical reaction with NBD-PC molecule making it non-fluorescent. After the addition of dithionite the fluorescence on single vesicles as well as on vesicle suspension showed a 50 % decrease of its initial value corresponding to marker quenching in the outer leaflet. Hence, flourimetry as well as flourescence microscopy prove that dithionite quenching is a suitable method to induce an asymmetrical labeling of the NBD-PC marked giant vesicles. Springer Online Journal Archives 1860-2002 Gomišček, Gregor oth Arrigler, Vesna oth Gros, Marjan oth Zupančič, Mirjana oth Svetina, Saša oth in Pflügers Archiv 1868 440(2000) vom: Juli, Seite r051 (DE-627)NLEJ188987363 (DE-600)1463014-x 1432-2013 nnns volume:440 year:2000 month:07 pages:r051 http://dx.doi.org/10.1007/s004240000003 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 440 2000 7 r051 |
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(DE-627)NLEJ201021277 DE-627 ger DE-627 rakwb eng Asymmetrical labeling of giant phospholipid vesicles 2000 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract The method for labeling of inner membrane leaflet in unilamellar giant POPC vesicles was developed and characterised. Symmetrically NBD-PC labeled vesicles were treated by sodium dithionite, which undergoes an irreversible chemical reaction with NBD-PC molecule making it non-fluorescent. After the addition of dithionite the fluorescence on single vesicles as well as on vesicle suspension showed a 50 % decrease of its initial value corresponding to marker quenching in the outer leaflet. Hence, flourimetry as well as flourescence microscopy prove that dithionite quenching is a suitable method to induce an asymmetrical labeling of the NBD-PC marked giant vesicles. Springer Online Journal Archives 1860-2002 Gomišček, Gregor oth Arrigler, Vesna oth Gros, Marjan oth Zupančič, Mirjana oth Svetina, Saša oth in Pflügers Archiv 1868 440(2000) vom: Juli, Seite r051 (DE-627)NLEJ188987363 (DE-600)1463014-x 1432-2013 nnns volume:440 year:2000 month:07 pages:r051 http://dx.doi.org/10.1007/s004240000003 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 440 2000 7 r051 |
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(DE-627)NLEJ201021277 DE-627 ger DE-627 rakwb eng Asymmetrical labeling of giant phospholipid vesicles 2000 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract The method for labeling of inner membrane leaflet in unilamellar giant POPC vesicles was developed and characterised. Symmetrically NBD-PC labeled vesicles were treated by sodium dithionite, which undergoes an irreversible chemical reaction with NBD-PC molecule making it non-fluorescent. After the addition of dithionite the fluorescence on single vesicles as well as on vesicle suspension showed a 50 % decrease of its initial value corresponding to marker quenching in the outer leaflet. Hence, flourimetry as well as flourescence microscopy prove that dithionite quenching is a suitable method to induce an asymmetrical labeling of the NBD-PC marked giant vesicles. Springer Online Journal Archives 1860-2002 Gomišček, Gregor oth Arrigler, Vesna oth Gros, Marjan oth Zupančič, Mirjana oth Svetina, Saša oth in Pflügers Archiv 1868 440(2000) vom: Juli, Seite r051 (DE-627)NLEJ188987363 (DE-600)1463014-x 1432-2013 nnns volume:440 year:2000 month:07 pages:r051 http://dx.doi.org/10.1007/s004240000003 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 440 2000 7 r051 |
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(DE-627)NLEJ201021277 DE-627 ger DE-627 rakwb eng Asymmetrical labeling of giant phospholipid vesicles 2000 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract The method for labeling of inner membrane leaflet in unilamellar giant POPC vesicles was developed and characterised. Symmetrically NBD-PC labeled vesicles were treated by sodium dithionite, which undergoes an irreversible chemical reaction with NBD-PC molecule making it non-fluorescent. After the addition of dithionite the fluorescence on single vesicles as well as on vesicle suspension showed a 50 % decrease of its initial value corresponding to marker quenching in the outer leaflet. Hence, flourimetry as well as flourescence microscopy prove that dithionite quenching is a suitable method to induce an asymmetrical labeling of the NBD-PC marked giant vesicles. Springer Online Journal Archives 1860-2002 Gomišček, Gregor oth Arrigler, Vesna oth Gros, Marjan oth Zupančič, Mirjana oth Svetina, Saša oth in Pflügers Archiv 1868 440(2000) vom: Juli, Seite r051 (DE-627)NLEJ188987363 (DE-600)1463014-x 1432-2013 nnns volume:440 year:2000 month:07 pages:r051 http://dx.doi.org/10.1007/s004240000003 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 440 2000 7 r051 |
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Abstract The method for labeling of inner membrane leaflet in unilamellar giant POPC vesicles was developed and characterised. Symmetrically NBD-PC labeled vesicles were treated by sodium dithionite, which undergoes an irreversible chemical reaction with NBD-PC molecule making it non-fluorescent. After the addition of dithionite the fluorescence on single vesicles as well as on vesicle suspension showed a 50 % decrease of its initial value corresponding to marker quenching in the outer leaflet. Hence, flourimetry as well as flourescence microscopy prove that dithionite quenching is a suitable method to induce an asymmetrical labeling of the NBD-PC marked giant vesicles. |
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Abstract The method for labeling of inner membrane leaflet in unilamellar giant POPC vesicles was developed and characterised. Symmetrically NBD-PC labeled vesicles were treated by sodium dithionite, which undergoes an irreversible chemical reaction with NBD-PC molecule making it non-fluorescent. After the addition of dithionite the fluorescence on single vesicles as well as on vesicle suspension showed a 50 % decrease of its initial value corresponding to marker quenching in the outer leaflet. Hence, flourimetry as well as flourescence microscopy prove that dithionite quenching is a suitable method to induce an asymmetrical labeling of the NBD-PC marked giant vesicles. |
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Abstract The method for labeling of inner membrane leaflet in unilamellar giant POPC vesicles was developed and characterised. Symmetrically NBD-PC labeled vesicles were treated by sodium dithionite, which undergoes an irreversible chemical reaction with NBD-PC molecule making it non-fluorescent. After the addition of dithionite the fluorescence on single vesicles as well as on vesicle suspension showed a 50 % decrease of its initial value corresponding to marker quenching in the outer leaflet. Hence, flourimetry as well as flourescence microscopy prove that dithionite quenching is a suitable method to induce an asymmetrical labeling of the NBD-PC marked giant vesicles. |
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