Met- and leu-enkephalins inhibit rat cortical neurons intracellularly recorded in vivo while morphine excites them: evidence for naloxone-sensitive and naloxone-insensitive effects
Summary The action of enkephalin-analogues (D-ala2-D-leu5-enkephalin and D-ala2-D-metenkephalin) and morphine, iontophoretically applied, was tested on rat cortical neurons intracellularly recorded “in vivo”. Inhibition of cellular excitability of 60% of the tested cells followed the iontophoretic a...
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E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1989 |
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| Umfang: |
7 |
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Springer Online Journal Archives 1860-2002 |
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| Übergeordnetes Werk: |
in: Experimental brain research - 1966, 77(1989) vom: Feb., Seite 302-308 |
| Übergeordnetes Werk: |
volume:77 ; year:1989 ; month:02 ; pages:302-308 ; extent:7 |
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| 520 | |a Summary The action of enkephalin-analogues (D-ala2-D-leu5-enkephalin and D-ala2-D-metenkephalin) and morphine, iontophoretically applied, was tested on rat cortical neurons intracellularly recorded “in vivo”. Inhibition of cellular excitability of 60% of the tested cells followed the iontophoretic administration of opioid peptides. 50% of the inhibited cells were also hyperpolarized. The amplitude of membrane hyperpolarization was related to the value of the membrane potential. In 13 out of the 30 inhibited cells the change in membrane input resistance was measured; the input resistance was decreased by 30%. In 8 cells, hyperpolarized by the opioid peptides, the depolarizing postsynaptic potentials, evoked by cortical stimulation, were also reduced in amplitude. Naloxone, iontophoretically applied, reversed and/or prevented the peptide responses. On the same neurons, morphine induced a bursting pattern of spiking activity and increased the membrane input resistance: this action was naloxone-insensitive. The reported results suggest that opioid peptides and morphine activate, respectively, naloxone-sensitive and naloxone-insensitive mechanisms on the same cortical neurons, leading to different and, in some respect, opposite effects on the neuronal activity. | ||
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(DE-627)NLEJ202294250 DE-627 ger DE-627 rakwb eng Met- and leu-enkephalins inhibit rat cortical neurons intracellularly recorded in vivo while morphine excites them: evidence for naloxone-sensitive and naloxone-insensitive effects 1989 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary The action of enkephalin-analogues (D-ala2-D-leu5-enkephalin and D-ala2-D-metenkephalin) and morphine, iontophoretically applied, was tested on rat cortical neurons intracellularly recorded “in vivo”. Inhibition of cellular excitability of 60% of the tested cells followed the iontophoretic administration of opioid peptides. 50% of the inhibited cells were also hyperpolarized. The amplitude of membrane hyperpolarization was related to the value of the membrane potential. In 13 out of the 30 inhibited cells the change in membrane input resistance was measured; the input resistance was decreased by 30%. In 8 cells, hyperpolarized by the opioid peptides, the depolarizing postsynaptic potentials, evoked by cortical stimulation, were also reduced in amplitude. Naloxone, iontophoretically applied, reversed and/or prevented the peptide responses. On the same neurons, morphine induced a bursting pattern of spiking activity and increased the membrane input resistance: this action was naloxone-insensitive. The reported results suggest that opioid peptides and morphine activate, respectively, naloxone-sensitive and naloxone-insensitive mechanisms on the same cortical neurons, leading to different and, in some respect, opposite effects on the neuronal activity. Springer Online Journal Archives 1860-2002 Stanzione, P. oth Stefani, A. oth Calabresi, P. oth Mercuri, N. B. oth Bernardi, G. oth in Experimental brain research 1966 77(1989) vom: Feb., Seite 302-308 (DE-627)NLEJ188991840 (DE-600)1459099-2 1432-1106 nnns volume:77 year:1989 month:02 pages:302-308 extent:7 http://dx.doi.org/10.1007/BF00274987 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 77 1989 2 302-308 7 |
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(DE-627)NLEJ202294250 DE-627 ger DE-627 rakwb eng Met- and leu-enkephalins inhibit rat cortical neurons intracellularly recorded in vivo while morphine excites them: evidence for naloxone-sensitive and naloxone-insensitive effects 1989 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary The action of enkephalin-analogues (D-ala2-D-leu5-enkephalin and D-ala2-D-metenkephalin) and morphine, iontophoretically applied, was tested on rat cortical neurons intracellularly recorded “in vivo”. Inhibition of cellular excitability of 60% of the tested cells followed the iontophoretic administration of opioid peptides. 50% of the inhibited cells were also hyperpolarized. The amplitude of membrane hyperpolarization was related to the value of the membrane potential. In 13 out of the 30 inhibited cells the change in membrane input resistance was measured; the input resistance was decreased by 30%. In 8 cells, hyperpolarized by the opioid peptides, the depolarizing postsynaptic potentials, evoked by cortical stimulation, were also reduced in amplitude. Naloxone, iontophoretically applied, reversed and/or prevented the peptide responses. On the same neurons, morphine induced a bursting pattern of spiking activity and increased the membrane input resistance: this action was naloxone-insensitive. The reported results suggest that opioid peptides and morphine activate, respectively, naloxone-sensitive and naloxone-insensitive mechanisms on the same cortical neurons, leading to different and, in some respect, opposite effects on the neuronal activity. Springer Online Journal Archives 1860-2002 Stanzione, P. oth Stefani, A. oth Calabresi, P. oth Mercuri, N. B. oth Bernardi, G. oth in Experimental brain research 1966 77(1989) vom: Feb., Seite 302-308 (DE-627)NLEJ188991840 (DE-600)1459099-2 1432-1106 nnns volume:77 year:1989 month:02 pages:302-308 extent:7 http://dx.doi.org/10.1007/BF00274987 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 77 1989 2 302-308 7 |
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(DE-627)NLEJ202294250 DE-627 ger DE-627 rakwb eng Met- and leu-enkephalins inhibit rat cortical neurons intracellularly recorded in vivo while morphine excites them: evidence for naloxone-sensitive and naloxone-insensitive effects 1989 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary The action of enkephalin-analogues (D-ala2-D-leu5-enkephalin and D-ala2-D-metenkephalin) and morphine, iontophoretically applied, was tested on rat cortical neurons intracellularly recorded “in vivo”. Inhibition of cellular excitability of 60% of the tested cells followed the iontophoretic administration of opioid peptides. 50% of the inhibited cells were also hyperpolarized. The amplitude of membrane hyperpolarization was related to the value of the membrane potential. In 13 out of the 30 inhibited cells the change in membrane input resistance was measured; the input resistance was decreased by 30%. In 8 cells, hyperpolarized by the opioid peptides, the depolarizing postsynaptic potentials, evoked by cortical stimulation, were also reduced in amplitude. Naloxone, iontophoretically applied, reversed and/or prevented the peptide responses. On the same neurons, morphine induced a bursting pattern of spiking activity and increased the membrane input resistance: this action was naloxone-insensitive. The reported results suggest that opioid peptides and morphine activate, respectively, naloxone-sensitive and naloxone-insensitive mechanisms on the same cortical neurons, leading to different and, in some respect, opposite effects on the neuronal activity. Springer Online Journal Archives 1860-2002 Stanzione, P. oth Stefani, A. oth Calabresi, P. oth Mercuri, N. B. oth Bernardi, G. oth in Experimental brain research 1966 77(1989) vom: Feb., Seite 302-308 (DE-627)NLEJ188991840 (DE-600)1459099-2 1432-1106 nnns volume:77 year:1989 month:02 pages:302-308 extent:7 http://dx.doi.org/10.1007/BF00274987 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 77 1989 2 302-308 7 |
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(DE-627)NLEJ202294250 DE-627 ger DE-627 rakwb eng Met- and leu-enkephalins inhibit rat cortical neurons intracellularly recorded in vivo while morphine excites them: evidence for naloxone-sensitive and naloxone-insensitive effects 1989 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary The action of enkephalin-analogues (D-ala2-D-leu5-enkephalin and D-ala2-D-metenkephalin) and morphine, iontophoretically applied, was tested on rat cortical neurons intracellularly recorded “in vivo”. Inhibition of cellular excitability of 60% of the tested cells followed the iontophoretic administration of opioid peptides. 50% of the inhibited cells were also hyperpolarized. The amplitude of membrane hyperpolarization was related to the value of the membrane potential. In 13 out of the 30 inhibited cells the change in membrane input resistance was measured; the input resistance was decreased by 30%. In 8 cells, hyperpolarized by the opioid peptides, the depolarizing postsynaptic potentials, evoked by cortical stimulation, were also reduced in amplitude. Naloxone, iontophoretically applied, reversed and/or prevented the peptide responses. On the same neurons, morphine induced a bursting pattern of spiking activity and increased the membrane input resistance: this action was naloxone-insensitive. The reported results suggest that opioid peptides and morphine activate, respectively, naloxone-sensitive and naloxone-insensitive mechanisms on the same cortical neurons, leading to different and, in some respect, opposite effects on the neuronal activity. Springer Online Journal Archives 1860-2002 Stanzione, P. oth Stefani, A. oth Calabresi, P. oth Mercuri, N. B. oth Bernardi, G. oth in Experimental brain research 1966 77(1989) vom: Feb., Seite 302-308 (DE-627)NLEJ188991840 (DE-600)1459099-2 1432-1106 nnns volume:77 year:1989 month:02 pages:302-308 extent:7 http://dx.doi.org/10.1007/BF00274987 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 77 1989 2 302-308 7 |
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Met- and leu-enkephalins inhibit rat cortical neurons intracellularly recorded in vivo while morphine excites them: evidence for naloxone-sensitive and naloxone-insensitive effects |
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Summary The action of enkephalin-analogues (D-ala2-D-leu5-enkephalin and D-ala2-D-metenkephalin) and morphine, iontophoretically applied, was tested on rat cortical neurons intracellularly recorded “in vivo”. Inhibition of cellular excitability of 60% of the tested cells followed the iontophoretic administration of opioid peptides. 50% of the inhibited cells were also hyperpolarized. The amplitude of membrane hyperpolarization was related to the value of the membrane potential. In 13 out of the 30 inhibited cells the change in membrane input resistance was measured; the input resistance was decreased by 30%. In 8 cells, hyperpolarized by the opioid peptides, the depolarizing postsynaptic potentials, evoked by cortical stimulation, were also reduced in amplitude. Naloxone, iontophoretically applied, reversed and/or prevented the peptide responses. On the same neurons, morphine induced a bursting pattern of spiking activity and increased the membrane input resistance: this action was naloxone-insensitive. The reported results suggest that opioid peptides and morphine activate, respectively, naloxone-sensitive and naloxone-insensitive mechanisms on the same cortical neurons, leading to different and, in some respect, opposite effects on the neuronal activity. |
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Summary The action of enkephalin-analogues (D-ala2-D-leu5-enkephalin and D-ala2-D-metenkephalin) and morphine, iontophoretically applied, was tested on rat cortical neurons intracellularly recorded “in vivo”. Inhibition of cellular excitability of 60% of the tested cells followed the iontophoretic administration of opioid peptides. 50% of the inhibited cells were also hyperpolarized. The amplitude of membrane hyperpolarization was related to the value of the membrane potential. In 13 out of the 30 inhibited cells the change in membrane input resistance was measured; the input resistance was decreased by 30%. In 8 cells, hyperpolarized by the opioid peptides, the depolarizing postsynaptic potentials, evoked by cortical stimulation, were also reduced in amplitude. Naloxone, iontophoretically applied, reversed and/or prevented the peptide responses. On the same neurons, morphine induced a bursting pattern of spiking activity and increased the membrane input resistance: this action was naloxone-insensitive. The reported results suggest that opioid peptides and morphine activate, respectively, naloxone-sensitive and naloxone-insensitive mechanisms on the same cortical neurons, leading to different and, in some respect, opposite effects on the neuronal activity. |
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Summary The action of enkephalin-analogues (D-ala2-D-leu5-enkephalin and D-ala2-D-metenkephalin) and morphine, iontophoretically applied, was tested on rat cortical neurons intracellularly recorded “in vivo”. Inhibition of cellular excitability of 60% of the tested cells followed the iontophoretic administration of opioid peptides. 50% of the inhibited cells were also hyperpolarized. The amplitude of membrane hyperpolarization was related to the value of the membrane potential. In 13 out of the 30 inhibited cells the change in membrane input resistance was measured; the input resistance was decreased by 30%. In 8 cells, hyperpolarized by the opioid peptides, the depolarizing postsynaptic potentials, evoked by cortical stimulation, were also reduced in amplitude. Naloxone, iontophoretically applied, reversed and/or prevented the peptide responses. On the same neurons, morphine induced a bursting pattern of spiking activity and increased the membrane input resistance: this action was naloxone-insensitive. The reported results suggest that opioid peptides and morphine activate, respectively, naloxone-sensitive and naloxone-insensitive mechanisms on the same cortical neurons, leading to different and, in some respect, opposite effects on the neuronal activity. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ202294250</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210706094845.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070527s1989 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ202294250</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Met- and leu-enkephalins inhibit rat cortical neurons intracellularly recorded in vivo while morphine excites them: evidence for naloxone-sensitive and naloxone-insensitive effects</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1989</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">7</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Summary The action of enkephalin-analogues (D-ala2-D-leu5-enkephalin and D-ala2-D-metenkephalin) and morphine, iontophoretically applied, was tested on rat cortical neurons intracellularly recorded “in vivo”. Inhibition of cellular excitability of 60% of the tested cells followed the iontophoretic administration of opioid peptides. 50% of the inhibited cells were also hyperpolarized. The amplitude of membrane hyperpolarization was related to the value of the membrane potential. In 13 out of the 30 inhibited cells the change in membrane input resistance was measured; the input resistance was decreased by 30%. In 8 cells, hyperpolarized by the opioid peptides, the depolarizing postsynaptic potentials, evoked by cortical stimulation, were also reduced in amplitude. Naloxone, iontophoretically applied, reversed and/or prevented the peptide responses. On the same neurons, morphine induced a bursting pattern of spiking activity and increased the membrane input resistance: this action was naloxone-insensitive. The reported results suggest that opioid peptides and morphine activate, respectively, naloxone-sensitive and naloxone-insensitive mechanisms on the same cortical neurons, leading to different and, in some respect, opposite effects on the neuronal activity.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Springer Online Journal Archives 1860-2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Stanzione, P.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Stefani, A.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Calabresi, P.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Mercuri, N. B.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Bernardi, G.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">Experimental brain research</subfield><subfield code="d">1966</subfield><subfield code="g">77(1989) vom: Feb., Seite 302-308</subfield><subfield code="w">(DE-627)NLEJ188991840</subfield><subfield code="w">(DE-600)1459099-2</subfield><subfield code="x">1432-1106</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:77</subfield><subfield code="g">year:1989</subfield><subfield code="g">month:02</subfield><subfield code="g">pages:302-308</subfield><subfield code="g">extent:7</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1007/BF00274987</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SOJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">77</subfield><subfield code="j">1989</subfield><subfield code="c">2</subfield><subfield code="h">302-308</subfield><subfield code="g">7</subfield></datafield></record></collection>
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