Cell-substrate separation: effect of applied force and temperature
Abstract We measure the change in cell-substrate separation in response to an upward force by combining two relatively new techniques, Electric Cell-substrate Impedance Sensing (ECIS) to measure average cell-substrate separation, and collagen-coated magnetic beads to apply force to the top (dorsal)...
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E-Artikel |
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Englisch |
| Erschienen: |
1998 |
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9 |
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Springer Online Journal Archives 1860-2002 |
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in: European biophysics journal - 1974, 27(1998) vom: Jan., Seite 9-17 |
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volume:27 ; year:1998 ; month:01 ; pages:9-17 ; extent:9 |
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| 520 | |a Abstract We measure the change in cell-substrate separation in response to an upward force by combining two relatively new techniques, Electric Cell-substrate Impedance Sensing (ECIS) to measure average cell-substrate separation, and collagen-coated magnetic beads to apply force to the top (dorsal) surface of cells. The collagen-coated ferric oxide beads attach to integrin receptors in the dorsal surfaces of osteoblastlike ROS 17/2.8 cells. Magnetic force is controlled by the position and the number of permanent magnets, applying an average 320 or 560 pN per cell. Comparing model calculations with experimental impedance data, the junctional resistivity of the cell layer and the average distance between the lower (ventral) cell surface and substrate can be determined. The ECIS analysis shows that these forces produce an increase in the distance between the ventral cell surface and the substrate that is in the range of 10 to 25%. At temperatures of 4°, 22° and 37 °C, the measured cell surface-substrate distances without magnetic beads are 84 ± 4, 45 ± 2 and 38 ± 2 nm. The force-induced changes at 22° are 11 ± 3 and 21 ± 3 nm for 320 and 560 pN, and at 37° they are 5 ± 2 and 9 ± 2 nm. The resulting cell-substrate spring constants at 22° and 37° are thus about 28 and 63 pN nm–1 (dyne cm–1). Using a reasonable range for the number for individual integrin-ligand adhesion bonds gives a range for the spring constant of the individual adhesion bond of from about 10–3 to 10–1 pN nm–1. These data also provide evidence that the number of adhesion bonds per cell increases with temperature. | ||
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| 700 | 1 | |a Rossi, Marisa |4 oth | |
| 700 | 1 | |a Ferrier, J. |4 oth | |
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(DE-627)NLEJ202662160 DE-627 ger DE-627 rakwb eng Cell-substrate separation: effect of applied force and temperature 1998 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract We measure the change in cell-substrate separation in response to an upward force by combining two relatively new techniques, Electric Cell-substrate Impedance Sensing (ECIS) to measure average cell-substrate separation, and collagen-coated magnetic beads to apply force to the top (dorsal) surface of cells. The collagen-coated ferric oxide beads attach to integrin receptors in the dorsal surfaces of osteoblastlike ROS 17/2.8 cells. Magnetic force is controlled by the position and the number of permanent magnets, applying an average 320 or 560 pN per cell. Comparing model calculations with experimental impedance data, the junctional resistivity of the cell layer and the average distance between the lower (ventral) cell surface and substrate can be determined. The ECIS analysis shows that these forces produce an increase in the distance between the ventral cell surface and the substrate that is in the range of 10 to 25%. At temperatures of 4°, 22° and 37 °C, the measured cell surface-substrate distances without magnetic beads are 84 ± 4, 45 ± 2 and 38 ± 2 nm. The force-induced changes at 22° are 11 ± 3 and 21 ± 3 nm for 320 and 560 pN, and at 37° they are 5 ± 2 and 9 ± 2 nm. The resulting cell-substrate spring constants at 22° and 37° are thus about 28 and 63 pN nm–1 (dyne cm–1). Using a reasonable range for the number for individual integrin-ligand adhesion bonds gives a range for the spring constant of the individual adhesion bond of from about 10–3 to 10–1 pN nm–1. These data also provide evidence that the number of adhesion bonds per cell increases with temperature. Springer Online Journal Archives 1860-2002 Lo, Chun-Min oth Glogauer, Michael oth Rossi, Marisa oth Ferrier, J. oth in European biophysics journal 1974 27(1998) vom: Jan., Seite 9-17 (DE-627)NLEJ188988947 (DE-600)1398349-0 1432-1017 nnns volume:27 year:1998 month:01 pages:9-17 extent:9 http://dx.doi.org/10.1007/s002490050105 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 27 1998 1 9-17 9 |
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(DE-627)NLEJ202662160 DE-627 ger DE-627 rakwb eng Cell-substrate separation: effect of applied force and temperature 1998 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract We measure the change in cell-substrate separation in response to an upward force by combining two relatively new techniques, Electric Cell-substrate Impedance Sensing (ECIS) to measure average cell-substrate separation, and collagen-coated magnetic beads to apply force to the top (dorsal) surface of cells. The collagen-coated ferric oxide beads attach to integrin receptors in the dorsal surfaces of osteoblastlike ROS 17/2.8 cells. Magnetic force is controlled by the position and the number of permanent magnets, applying an average 320 or 560 pN per cell. Comparing model calculations with experimental impedance data, the junctional resistivity of the cell layer and the average distance between the lower (ventral) cell surface and substrate can be determined. The ECIS analysis shows that these forces produce an increase in the distance between the ventral cell surface and the substrate that is in the range of 10 to 25%. At temperatures of 4°, 22° and 37 °C, the measured cell surface-substrate distances without magnetic beads are 84 ± 4, 45 ± 2 and 38 ± 2 nm. The force-induced changes at 22° are 11 ± 3 and 21 ± 3 nm for 320 and 560 pN, and at 37° they are 5 ± 2 and 9 ± 2 nm. The resulting cell-substrate spring constants at 22° and 37° are thus about 28 and 63 pN nm–1 (dyne cm–1). Using a reasonable range for the number for individual integrin-ligand adhesion bonds gives a range for the spring constant of the individual adhesion bond of from about 10–3 to 10–1 pN nm–1. These data also provide evidence that the number of adhesion bonds per cell increases with temperature. Springer Online Journal Archives 1860-2002 Lo, Chun-Min oth Glogauer, Michael oth Rossi, Marisa oth Ferrier, J. oth in European biophysics journal 1974 27(1998) vom: Jan., Seite 9-17 (DE-627)NLEJ188988947 (DE-600)1398349-0 1432-1017 nnns volume:27 year:1998 month:01 pages:9-17 extent:9 http://dx.doi.org/10.1007/s002490050105 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 27 1998 1 9-17 9 |
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(DE-627)NLEJ202662160 DE-627 ger DE-627 rakwb eng Cell-substrate separation: effect of applied force and temperature 1998 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract We measure the change in cell-substrate separation in response to an upward force by combining two relatively new techniques, Electric Cell-substrate Impedance Sensing (ECIS) to measure average cell-substrate separation, and collagen-coated magnetic beads to apply force to the top (dorsal) surface of cells. The collagen-coated ferric oxide beads attach to integrin receptors in the dorsal surfaces of osteoblastlike ROS 17/2.8 cells. Magnetic force is controlled by the position and the number of permanent magnets, applying an average 320 or 560 pN per cell. Comparing model calculations with experimental impedance data, the junctional resistivity of the cell layer and the average distance between the lower (ventral) cell surface and substrate can be determined. The ECIS analysis shows that these forces produce an increase in the distance between the ventral cell surface and the substrate that is in the range of 10 to 25%. At temperatures of 4°, 22° and 37 °C, the measured cell surface-substrate distances without magnetic beads are 84 ± 4, 45 ± 2 and 38 ± 2 nm. The force-induced changes at 22° are 11 ± 3 and 21 ± 3 nm for 320 and 560 pN, and at 37° they are 5 ± 2 and 9 ± 2 nm. The resulting cell-substrate spring constants at 22° and 37° are thus about 28 and 63 pN nm–1 (dyne cm–1). Using a reasonable range for the number for individual integrin-ligand adhesion bonds gives a range for the spring constant of the individual adhesion bond of from about 10–3 to 10–1 pN nm–1. These data also provide evidence that the number of adhesion bonds per cell increases with temperature. Springer Online Journal Archives 1860-2002 Lo, Chun-Min oth Glogauer, Michael oth Rossi, Marisa oth Ferrier, J. oth in European biophysics journal 1974 27(1998) vom: Jan., Seite 9-17 (DE-627)NLEJ188988947 (DE-600)1398349-0 1432-1017 nnns volume:27 year:1998 month:01 pages:9-17 extent:9 http://dx.doi.org/10.1007/s002490050105 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 27 1998 1 9-17 9 |
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(DE-627)NLEJ202662160 DE-627 ger DE-627 rakwb eng Cell-substrate separation: effect of applied force and temperature 1998 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract We measure the change in cell-substrate separation in response to an upward force by combining two relatively new techniques, Electric Cell-substrate Impedance Sensing (ECIS) to measure average cell-substrate separation, and collagen-coated magnetic beads to apply force to the top (dorsal) surface of cells. The collagen-coated ferric oxide beads attach to integrin receptors in the dorsal surfaces of osteoblastlike ROS 17/2.8 cells. Magnetic force is controlled by the position and the number of permanent magnets, applying an average 320 or 560 pN per cell. Comparing model calculations with experimental impedance data, the junctional resistivity of the cell layer and the average distance between the lower (ventral) cell surface and substrate can be determined. The ECIS analysis shows that these forces produce an increase in the distance between the ventral cell surface and the substrate that is in the range of 10 to 25%. At temperatures of 4°, 22° and 37 °C, the measured cell surface-substrate distances without magnetic beads are 84 ± 4, 45 ± 2 and 38 ± 2 nm. The force-induced changes at 22° are 11 ± 3 and 21 ± 3 nm for 320 and 560 pN, and at 37° they are 5 ± 2 and 9 ± 2 nm. The resulting cell-substrate spring constants at 22° and 37° are thus about 28 and 63 pN nm–1 (dyne cm–1). Using a reasonable range for the number for individual integrin-ligand adhesion bonds gives a range for the spring constant of the individual adhesion bond of from about 10–3 to 10–1 pN nm–1. These data also provide evidence that the number of adhesion bonds per cell increases with temperature. Springer Online Journal Archives 1860-2002 Lo, Chun-Min oth Glogauer, Michael oth Rossi, Marisa oth Ferrier, J. oth in European biophysics journal 1974 27(1998) vom: Jan., Seite 9-17 (DE-627)NLEJ188988947 (DE-600)1398349-0 1432-1017 nnns volume:27 year:1998 month:01 pages:9-17 extent:9 http://dx.doi.org/10.1007/s002490050105 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 27 1998 1 9-17 9 |
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(DE-627)NLEJ202662160 DE-627 ger DE-627 rakwb eng Cell-substrate separation: effect of applied force and temperature 1998 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract We measure the change in cell-substrate separation in response to an upward force by combining two relatively new techniques, Electric Cell-substrate Impedance Sensing (ECIS) to measure average cell-substrate separation, and collagen-coated magnetic beads to apply force to the top (dorsal) surface of cells. The collagen-coated ferric oxide beads attach to integrin receptors in the dorsal surfaces of osteoblastlike ROS 17/2.8 cells. Magnetic force is controlled by the position and the number of permanent magnets, applying an average 320 or 560 pN per cell. Comparing model calculations with experimental impedance data, the junctional resistivity of the cell layer and the average distance between the lower (ventral) cell surface and substrate can be determined. The ECIS analysis shows that these forces produce an increase in the distance between the ventral cell surface and the substrate that is in the range of 10 to 25%. At temperatures of 4°, 22° and 37 °C, the measured cell surface-substrate distances without magnetic beads are 84 ± 4, 45 ± 2 and 38 ± 2 nm. The force-induced changes at 22° are 11 ± 3 and 21 ± 3 nm for 320 and 560 pN, and at 37° they are 5 ± 2 and 9 ± 2 nm. The resulting cell-substrate spring constants at 22° and 37° are thus about 28 and 63 pN nm–1 (dyne cm–1). Using a reasonable range for the number for individual integrin-ligand adhesion bonds gives a range for the spring constant of the individual adhesion bond of from about 10–3 to 10–1 pN nm–1. These data also provide evidence that the number of adhesion bonds per cell increases with temperature. Springer Online Journal Archives 1860-2002 Lo, Chun-Min oth Glogauer, Michael oth Rossi, Marisa oth Ferrier, J. oth in European biophysics journal 1974 27(1998) vom: Jan., Seite 9-17 (DE-627)NLEJ188988947 (DE-600)1398349-0 1432-1017 nnns volume:27 year:1998 month:01 pages:9-17 extent:9 http://dx.doi.org/10.1007/s002490050105 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 27 1998 1 9-17 9 |
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Abstract We measure the change in cell-substrate separation in response to an upward force by combining two relatively new techniques, Electric Cell-substrate Impedance Sensing (ECIS) to measure average cell-substrate separation, and collagen-coated magnetic beads to apply force to the top (dorsal) surface of cells. The collagen-coated ferric oxide beads attach to integrin receptors in the dorsal surfaces of osteoblastlike ROS 17/2.8 cells. Magnetic force is controlled by the position and the number of permanent magnets, applying an average 320 or 560 pN per cell. Comparing model calculations with experimental impedance data, the junctional resistivity of the cell layer and the average distance between the lower (ventral) cell surface and substrate can be determined. The ECIS analysis shows that these forces produce an increase in the distance between the ventral cell surface and the substrate that is in the range of 10 to 25%. At temperatures of 4°, 22° and 37 °C, the measured cell surface-substrate distances without magnetic beads are 84 ± 4, 45 ± 2 and 38 ± 2 nm. The force-induced changes at 22° are 11 ± 3 and 21 ± 3 nm for 320 and 560 pN, and at 37° they are 5 ± 2 and 9 ± 2 nm. The resulting cell-substrate spring constants at 22° and 37° are thus about 28 and 63 pN nm–1 (dyne cm–1). Using a reasonable range for the number for individual integrin-ligand adhesion bonds gives a range for the spring constant of the individual adhesion bond of from about 10–3 to 10–1 pN nm–1. These data also provide evidence that the number of adhesion bonds per cell increases with temperature. |
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Abstract We measure the change in cell-substrate separation in response to an upward force by combining two relatively new techniques, Electric Cell-substrate Impedance Sensing (ECIS) to measure average cell-substrate separation, and collagen-coated magnetic beads to apply force to the top (dorsal) surface of cells. The collagen-coated ferric oxide beads attach to integrin receptors in the dorsal surfaces of osteoblastlike ROS 17/2.8 cells. Magnetic force is controlled by the position and the number of permanent magnets, applying an average 320 or 560 pN per cell. Comparing model calculations with experimental impedance data, the junctional resistivity of the cell layer and the average distance between the lower (ventral) cell surface and substrate can be determined. The ECIS analysis shows that these forces produce an increase in the distance between the ventral cell surface and the substrate that is in the range of 10 to 25%. At temperatures of 4°, 22° and 37 °C, the measured cell surface-substrate distances without magnetic beads are 84 ± 4, 45 ± 2 and 38 ± 2 nm. The force-induced changes at 22° are 11 ± 3 and 21 ± 3 nm for 320 and 560 pN, and at 37° they are 5 ± 2 and 9 ± 2 nm. The resulting cell-substrate spring constants at 22° and 37° are thus about 28 and 63 pN nm–1 (dyne cm–1). Using a reasonable range for the number for individual integrin-ligand adhesion bonds gives a range for the spring constant of the individual adhesion bond of from about 10–3 to 10–1 pN nm–1. These data also provide evidence that the number of adhesion bonds per cell increases with temperature. |
| abstract_unstemmed |
Abstract We measure the change in cell-substrate separation in response to an upward force by combining two relatively new techniques, Electric Cell-substrate Impedance Sensing (ECIS) to measure average cell-substrate separation, and collagen-coated magnetic beads to apply force to the top (dorsal) surface of cells. The collagen-coated ferric oxide beads attach to integrin receptors in the dorsal surfaces of osteoblastlike ROS 17/2.8 cells. Magnetic force is controlled by the position and the number of permanent magnets, applying an average 320 or 560 pN per cell. Comparing model calculations with experimental impedance data, the junctional resistivity of the cell layer and the average distance between the lower (ventral) cell surface and substrate can be determined. The ECIS analysis shows that these forces produce an increase in the distance between the ventral cell surface and the substrate that is in the range of 10 to 25%. At temperatures of 4°, 22° and 37 °C, the measured cell surface-substrate distances without magnetic beads are 84 ± 4, 45 ± 2 and 38 ± 2 nm. The force-induced changes at 22° are 11 ± 3 and 21 ± 3 nm for 320 and 560 pN, and at 37° they are 5 ± 2 and 9 ± 2 nm. The resulting cell-substrate spring constants at 22° and 37° are thus about 28 and 63 pN nm–1 (dyne cm–1). Using a reasonable range for the number for individual integrin-ligand adhesion bonds gives a range for the spring constant of the individual adhesion bond of from about 10–3 to 10–1 pN nm–1. These data also provide evidence that the number of adhesion bonds per cell increases with temperature. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ202662160</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210706104847.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070528s1998 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ202662160</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Cell-substrate separation: effect of applied force and temperature</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1998</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">9</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract We measure the change in cell-substrate separation in response to an upward force by combining two relatively new techniques, Electric Cell-substrate Impedance Sensing (ECIS) to measure average cell-substrate separation, and collagen-coated magnetic beads to apply force to the top (dorsal) surface of cells. The collagen-coated ferric oxide beads attach to integrin receptors in the dorsal surfaces of osteoblastlike ROS 17/2.8 cells. Magnetic force is controlled by the position and the number of permanent magnets, applying an average 320 or 560 pN per cell. Comparing model calculations with experimental impedance data, the junctional resistivity of the cell layer and the average distance between the lower (ventral) cell surface and substrate can be determined. The ECIS analysis shows that these forces produce an increase in the distance between the ventral cell surface and the substrate that is in the range of 10 to 25%. At temperatures of 4°, 22° and 37 °C, the measured cell surface-substrate distances without magnetic beads are 84 ± 4, 45 ± 2 and 38 ± 2 nm. The force-induced changes at 22° are 11 ± 3 and 21 ± 3 nm for 320 and 560 pN, and at 37° they are 5 ± 2 and 9 ± 2 nm. The resulting cell-substrate spring constants at 22° and 37° are thus about 28 and 63 pN nm–1 (dyne cm–1). Using a reasonable range for the number for individual integrin-ligand adhesion bonds gives a range for the spring constant of the individual adhesion bond of from about 10–3 to 10–1 pN nm–1. These data also provide evidence that the number of adhesion bonds per cell increases with temperature.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Springer Online Journal Archives 1860-2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Lo, Chun-Min</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Glogauer, Michael</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Rossi, Marisa</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Ferrier, J.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">European biophysics journal</subfield><subfield code="d">1974</subfield><subfield code="g">27(1998) vom: Jan., Seite 9-17</subfield><subfield code="w">(DE-627)NLEJ188988947</subfield><subfield code="w">(DE-600)1398349-0</subfield><subfield code="x">1432-1017</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:27</subfield><subfield code="g">year:1998</subfield><subfield code="g">month:01</subfield><subfield code="g">pages:9-17</subfield><subfield code="g">extent:9</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1007/s002490050105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SOJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">27</subfield><subfield code="j">1998</subfield><subfield code="c">1</subfield><subfield code="h">9-17</subfield><subfield code="g">9</subfield></datafield></record></collection>
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