Protein-specific cytotoxic T lymphocytes. Recognition of transfectants expressing intracellular, membrane-associated or secreted forms of β-galactosidase
Abstract BALB/c-derived tumor cells were transfected with recombinantEscherichia coli β-galactosidase (β-gal) genes which were inserted into IgM heavy chain gene derivatives, leading to expression of the resulting fusion protein in different cellular compartments. A β-gal-specific, major histocompat...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1989 |
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| Umfang: |
7 |
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| Reproduktion: |
Springer Online Journal Archives 1860-2002 |
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| Übergeordnetes Werk: |
in: Immunogenetics - 1974, 30(1989) vom: Apr., Seite 296-302 |
| Übergeordnetes Werk: |
volume:30 ; year:1989 ; month:04 ; pages:296-302 ; extent:7 |
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NLEJ202684547 |
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| 520 | |a Abstract BALB/c-derived tumor cells were transfected with recombinantEscherichia coli β-galactosidase (β-gal) genes which were inserted into IgM heavy chain gene derivatives, leading to expression of the resulting fusion protein in different cellular compartments. A β-gal-specific, major histocompatibility complex (MHC) class I-restricted CD8+CD4− cytotoxic T lymphocyte (CTL) line of BALB/c origin raised against one transfectant expressing cytoplasmic β-gal also lysed transfectants expressing β-gal as membrane-inserted fusion protein, as well as transfectants secreting β-gal. Our data show that MHC class I-restricted CTL can recognize fragments of nonviral cellular proteins, be they expressed as intracellular, membrane-inserted, or secreted products. The findings confirm and extend a hypothesis on the nature of minor histocompatibility (H) antigens formulated earlier. | ||
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(DE-627)NLEJ202684547 DE-627 ger DE-627 rakwb eng Protein-specific cytotoxic T lymphocytes. Recognition of transfectants expressing intracellular, membrane-associated or secreted forms of β-galactosidase 1989 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract BALB/c-derived tumor cells were transfected with recombinantEscherichia coli β-galactosidase (β-gal) genes which were inserted into IgM heavy chain gene derivatives, leading to expression of the resulting fusion protein in different cellular compartments. A β-gal-specific, major histocompatibility complex (MHC) class I-restricted CD8+CD4− cytotoxic T lymphocyte (CTL) line of BALB/c origin raised against one transfectant expressing cytoplasmic β-gal also lysed transfectants expressing β-gal as membrane-inserted fusion protein, as well as transfectants secreting β-gal. Our data show that MHC class I-restricted CTL can recognize fragments of nonviral cellular proteins, be they expressed as intracellular, membrane-inserted, or secreted products. The findings confirm and extend a hypothesis on the nature of minor histocompatibility (H) antigens formulated earlier. Springer Online Journal Archives 1860-2002 Rammensee, Hans-Georg oth Schild, Hansjörg oth Theopold, Ulrich oth in Immunogenetics 1974 30(1989) vom: Apr., Seite 296-302 (DE-627)NLEJ188990437 (DE-600)1398344-1 1432-1211 nnns volume:30 year:1989 month:04 pages:296-302 extent:7 http://dx.doi.org/10.1007/BF02421334 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 30 1989 4 296-302 7 |
| spelling |
(DE-627)NLEJ202684547 DE-627 ger DE-627 rakwb eng Protein-specific cytotoxic T lymphocytes. Recognition of transfectants expressing intracellular, membrane-associated or secreted forms of β-galactosidase 1989 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract BALB/c-derived tumor cells were transfected with recombinantEscherichia coli β-galactosidase (β-gal) genes which were inserted into IgM heavy chain gene derivatives, leading to expression of the resulting fusion protein in different cellular compartments. A β-gal-specific, major histocompatibility complex (MHC) class I-restricted CD8+CD4− cytotoxic T lymphocyte (CTL) line of BALB/c origin raised against one transfectant expressing cytoplasmic β-gal also lysed transfectants expressing β-gal as membrane-inserted fusion protein, as well as transfectants secreting β-gal. Our data show that MHC class I-restricted CTL can recognize fragments of nonviral cellular proteins, be they expressed as intracellular, membrane-inserted, or secreted products. The findings confirm and extend a hypothesis on the nature of minor histocompatibility (H) antigens formulated earlier. Springer Online Journal Archives 1860-2002 Rammensee, Hans-Georg oth Schild, Hansjörg oth Theopold, Ulrich oth in Immunogenetics 1974 30(1989) vom: Apr., Seite 296-302 (DE-627)NLEJ188990437 (DE-600)1398344-1 1432-1211 nnns volume:30 year:1989 month:04 pages:296-302 extent:7 http://dx.doi.org/10.1007/BF02421334 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 30 1989 4 296-302 7 |
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(DE-627)NLEJ202684547 DE-627 ger DE-627 rakwb eng Protein-specific cytotoxic T lymphocytes. Recognition of transfectants expressing intracellular, membrane-associated or secreted forms of β-galactosidase 1989 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract BALB/c-derived tumor cells were transfected with recombinantEscherichia coli β-galactosidase (β-gal) genes which were inserted into IgM heavy chain gene derivatives, leading to expression of the resulting fusion protein in different cellular compartments. A β-gal-specific, major histocompatibility complex (MHC) class I-restricted CD8+CD4− cytotoxic T lymphocyte (CTL) line of BALB/c origin raised against one transfectant expressing cytoplasmic β-gal also lysed transfectants expressing β-gal as membrane-inserted fusion protein, as well as transfectants secreting β-gal. Our data show that MHC class I-restricted CTL can recognize fragments of nonviral cellular proteins, be they expressed as intracellular, membrane-inserted, or secreted products. The findings confirm and extend a hypothesis on the nature of minor histocompatibility (H) antigens formulated earlier. Springer Online Journal Archives 1860-2002 Rammensee, Hans-Georg oth Schild, Hansjörg oth Theopold, Ulrich oth in Immunogenetics 1974 30(1989) vom: Apr., Seite 296-302 (DE-627)NLEJ188990437 (DE-600)1398344-1 1432-1211 nnns volume:30 year:1989 month:04 pages:296-302 extent:7 http://dx.doi.org/10.1007/BF02421334 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 30 1989 4 296-302 7 |
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(DE-627)NLEJ202684547 DE-627 ger DE-627 rakwb eng Protein-specific cytotoxic T lymphocytes. Recognition of transfectants expressing intracellular, membrane-associated or secreted forms of β-galactosidase 1989 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract BALB/c-derived tumor cells were transfected with recombinantEscherichia coli β-galactosidase (β-gal) genes which were inserted into IgM heavy chain gene derivatives, leading to expression of the resulting fusion protein in different cellular compartments. A β-gal-specific, major histocompatibility complex (MHC) class I-restricted CD8+CD4− cytotoxic T lymphocyte (CTL) line of BALB/c origin raised against one transfectant expressing cytoplasmic β-gal also lysed transfectants expressing β-gal as membrane-inserted fusion protein, as well as transfectants secreting β-gal. Our data show that MHC class I-restricted CTL can recognize fragments of nonviral cellular proteins, be they expressed as intracellular, membrane-inserted, or secreted products. The findings confirm and extend a hypothesis on the nature of minor histocompatibility (H) antigens formulated earlier. Springer Online Journal Archives 1860-2002 Rammensee, Hans-Georg oth Schild, Hansjörg oth Theopold, Ulrich oth in Immunogenetics 1974 30(1989) vom: Apr., Seite 296-302 (DE-627)NLEJ188990437 (DE-600)1398344-1 1432-1211 nnns volume:30 year:1989 month:04 pages:296-302 extent:7 http://dx.doi.org/10.1007/BF02421334 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 30 1989 4 296-302 7 |
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(DE-627)NLEJ202684547 DE-627 ger DE-627 rakwb eng Protein-specific cytotoxic T lymphocytes. Recognition of transfectants expressing intracellular, membrane-associated or secreted forms of β-galactosidase 1989 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract BALB/c-derived tumor cells were transfected with recombinantEscherichia coli β-galactosidase (β-gal) genes which were inserted into IgM heavy chain gene derivatives, leading to expression of the resulting fusion protein in different cellular compartments. A β-gal-specific, major histocompatibility complex (MHC) class I-restricted CD8+CD4− cytotoxic T lymphocyte (CTL) line of BALB/c origin raised against one transfectant expressing cytoplasmic β-gal also lysed transfectants expressing β-gal as membrane-inserted fusion protein, as well as transfectants secreting β-gal. Our data show that MHC class I-restricted CTL can recognize fragments of nonviral cellular proteins, be they expressed as intracellular, membrane-inserted, or secreted products. The findings confirm and extend a hypothesis on the nature of minor histocompatibility (H) antigens formulated earlier. Springer Online Journal Archives 1860-2002 Rammensee, Hans-Georg oth Schild, Hansjörg oth Theopold, Ulrich oth in Immunogenetics 1974 30(1989) vom: Apr., Seite 296-302 (DE-627)NLEJ188990437 (DE-600)1398344-1 1432-1211 nnns volume:30 year:1989 month:04 pages:296-302 extent:7 http://dx.doi.org/10.1007/BF02421334 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 30 1989 4 296-302 7 |
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in Immunogenetics 30(1989) vom: Apr., Seite 296-302 volume:30 year:1989 month:04 pages:296-302 extent:7 |
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protein-specific cytotoxic t lymphocytes. recognition of transfectants expressing intracellular, membrane-associated or secreted forms of β-galactosidase |
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Protein-specific cytotoxic T lymphocytes. Recognition of transfectants expressing intracellular, membrane-associated or secreted forms of β-galactosidase |
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Abstract BALB/c-derived tumor cells were transfected with recombinantEscherichia coli β-galactosidase (β-gal) genes which were inserted into IgM heavy chain gene derivatives, leading to expression of the resulting fusion protein in different cellular compartments. A β-gal-specific, major histocompatibility complex (MHC) class I-restricted CD8+CD4− cytotoxic T lymphocyte (CTL) line of BALB/c origin raised against one transfectant expressing cytoplasmic β-gal also lysed transfectants expressing β-gal as membrane-inserted fusion protein, as well as transfectants secreting β-gal. Our data show that MHC class I-restricted CTL can recognize fragments of nonviral cellular proteins, be they expressed as intracellular, membrane-inserted, or secreted products. The findings confirm and extend a hypothesis on the nature of minor histocompatibility (H) antigens formulated earlier. |
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Abstract BALB/c-derived tumor cells were transfected with recombinantEscherichia coli β-galactosidase (β-gal) genes which were inserted into IgM heavy chain gene derivatives, leading to expression of the resulting fusion protein in different cellular compartments. A β-gal-specific, major histocompatibility complex (MHC) class I-restricted CD8+CD4− cytotoxic T lymphocyte (CTL) line of BALB/c origin raised against one transfectant expressing cytoplasmic β-gal also lysed transfectants expressing β-gal as membrane-inserted fusion protein, as well as transfectants secreting β-gal. Our data show that MHC class I-restricted CTL can recognize fragments of nonviral cellular proteins, be they expressed as intracellular, membrane-inserted, or secreted products. The findings confirm and extend a hypothesis on the nature of minor histocompatibility (H) antigens formulated earlier. |
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Abstract BALB/c-derived tumor cells were transfected with recombinantEscherichia coli β-galactosidase (β-gal) genes which were inserted into IgM heavy chain gene derivatives, leading to expression of the resulting fusion protein in different cellular compartments. A β-gal-specific, major histocompatibility complex (MHC) class I-restricted CD8+CD4− cytotoxic T lymphocyte (CTL) line of BALB/c origin raised against one transfectant expressing cytoplasmic β-gal also lysed transfectants expressing β-gal as membrane-inserted fusion protein, as well as transfectants secreting β-gal. Our data show that MHC class I-restricted CTL can recognize fragments of nonviral cellular proteins, be they expressed as intracellular, membrane-inserted, or secreted products. The findings confirm and extend a hypothesis on the nature of minor histocompatibility (H) antigens formulated earlier. |
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