Analysis of early fetal T-cell receptor δ chain in humans
Abstract In the mouse it has been found that a number of T-cell receptor (Tcr) gd phenotypes are generated during fetal thymic development. To examine whether such “waves” of Tcrgd phenotypes can be found in man, we studied the V-region usage and junctional diversity of the T-cell receptor δ chain i...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1990 |
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| Umfang: |
6 |
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| Reproduktion: |
Springer Online Journal Archives 1860-2002 |
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| Übergeordnetes Werk: |
in: Immunogenetics - 1974, 32(1990) vom: Mai, Seite 331-336 |
| Übergeordnetes Werk: |
volume:32 ; year:1990 ; month:05 ; pages:331-336 ; extent:6 |
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| 520 | |a Abstract In the mouse it has been found that a number of T-cell receptor (Tcr) gd phenotypes are generated during fetal thymic development. To examine whether such “waves” of Tcrgd phenotypes can be found in man, we studied the V-region usage and junctional diversity of the T-cell receptor δ chain in human fetal and post-partum thymocytes and peripheral blood T cells. Using the polymerase chain reaction (PCR)-amplification technique it was found that in fetal thymocytes of 15–17 weeks of gestation the Tcrd-V3 gene segment was mainly employed, whereas in post-partum thymocytes the Tcrd-V1 gene segment was preferentially used. These Tcrd-V3 transcripts contained only a single D element (Dδ3) and a limited random nucleotide insertion. The Dδ3 element was also present in Tcrd-V3-containing transcripts derived from peripheral blood γδ Tcr+ clones. These data suggest that a wave of Tcr γδ might exist early in human fetal development that preferentially use the Tcrd-V3 gene segment. | ||
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| 700 | 1 | |a Bruining, Jan |4 oth | |
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| 700 | 1 | |a Elsen, Peter |4 oth | |
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(DE-627)NLEJ202686132 DE-627 ger DE-627 rakwb eng Analysis of early fetal T-cell receptor δ chain in humans 1990 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract In the mouse it has been found that a number of T-cell receptor (Tcr) gd phenotypes are generated during fetal thymic development. To examine whether such “waves” of Tcrgd phenotypes can be found in man, we studied the V-region usage and junctional diversity of the T-cell receptor δ chain in human fetal and post-partum thymocytes and peripheral blood T cells. Using the polymerase chain reaction (PCR)-amplification technique it was found that in fetal thymocytes of 15–17 weeks of gestation the Tcrd-V3 gene segment was mainly employed, whereas in post-partum thymocytes the Tcrd-V1 gene segment was preferentially used. These Tcrd-V3 transcripts contained only a single D element (Dδ3) and a limited random nucleotide insertion. The Dδ3 element was also present in Tcrd-V3-containing transcripts derived from peripheral blood γδ Tcr+ clones. These data suggest that a wave of Tcr γδ might exist early in human fetal development that preferentially use the Tcrd-V3 gene segment. Springer Online Journal Archives 1860-2002 Stoep, Nienke oth Krijger, Ronald oth Bruining, Jan oth Koning, Frits oth Elsen, Peter oth in Immunogenetics 1974 32(1990) vom: Mai, Seite 331-336 (DE-627)NLEJ188990437 (DE-600)1398344-1 1432-1211 nnns volume:32 year:1990 month:05 pages:331-336 extent:6 http://dx.doi.org/10.1007/BF00211647 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 32 1990 5 331-336 6 |
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(DE-627)NLEJ202686132 DE-627 ger DE-627 rakwb eng Analysis of early fetal T-cell receptor δ chain in humans 1990 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract In the mouse it has been found that a number of T-cell receptor (Tcr) gd phenotypes are generated during fetal thymic development. To examine whether such “waves” of Tcrgd phenotypes can be found in man, we studied the V-region usage and junctional diversity of the T-cell receptor δ chain in human fetal and post-partum thymocytes and peripheral blood T cells. Using the polymerase chain reaction (PCR)-amplification technique it was found that in fetal thymocytes of 15–17 weeks of gestation the Tcrd-V3 gene segment was mainly employed, whereas in post-partum thymocytes the Tcrd-V1 gene segment was preferentially used. These Tcrd-V3 transcripts contained only a single D element (Dδ3) and a limited random nucleotide insertion. The Dδ3 element was also present in Tcrd-V3-containing transcripts derived from peripheral blood γδ Tcr+ clones. These data suggest that a wave of Tcr γδ might exist early in human fetal development that preferentially use the Tcrd-V3 gene segment. Springer Online Journal Archives 1860-2002 Stoep, Nienke oth Krijger, Ronald oth Bruining, Jan oth Koning, Frits oth Elsen, Peter oth in Immunogenetics 1974 32(1990) vom: Mai, Seite 331-336 (DE-627)NLEJ188990437 (DE-600)1398344-1 1432-1211 nnns volume:32 year:1990 month:05 pages:331-336 extent:6 http://dx.doi.org/10.1007/BF00211647 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 32 1990 5 331-336 6 |
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(DE-627)NLEJ202686132 DE-627 ger DE-627 rakwb eng Analysis of early fetal T-cell receptor δ chain in humans 1990 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract In the mouse it has been found that a number of T-cell receptor (Tcr) gd phenotypes are generated during fetal thymic development. To examine whether such “waves” of Tcrgd phenotypes can be found in man, we studied the V-region usage and junctional diversity of the T-cell receptor δ chain in human fetal and post-partum thymocytes and peripheral blood T cells. Using the polymerase chain reaction (PCR)-amplification technique it was found that in fetal thymocytes of 15–17 weeks of gestation the Tcrd-V3 gene segment was mainly employed, whereas in post-partum thymocytes the Tcrd-V1 gene segment was preferentially used. These Tcrd-V3 transcripts contained only a single D element (Dδ3) and a limited random nucleotide insertion. The Dδ3 element was also present in Tcrd-V3-containing transcripts derived from peripheral blood γδ Tcr+ clones. These data suggest that a wave of Tcr γδ might exist early in human fetal development that preferentially use the Tcrd-V3 gene segment. Springer Online Journal Archives 1860-2002 Stoep, Nienke oth Krijger, Ronald oth Bruining, Jan oth Koning, Frits oth Elsen, Peter oth in Immunogenetics 1974 32(1990) vom: Mai, Seite 331-336 (DE-627)NLEJ188990437 (DE-600)1398344-1 1432-1211 nnns volume:32 year:1990 month:05 pages:331-336 extent:6 http://dx.doi.org/10.1007/BF00211647 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 32 1990 5 331-336 6 |
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(DE-627)NLEJ202686132 DE-627 ger DE-627 rakwb eng Analysis of early fetal T-cell receptor δ chain in humans 1990 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract In the mouse it has been found that a number of T-cell receptor (Tcr) gd phenotypes are generated during fetal thymic development. To examine whether such “waves” of Tcrgd phenotypes can be found in man, we studied the V-region usage and junctional diversity of the T-cell receptor δ chain in human fetal and post-partum thymocytes and peripheral blood T cells. Using the polymerase chain reaction (PCR)-amplification technique it was found that in fetal thymocytes of 15–17 weeks of gestation the Tcrd-V3 gene segment was mainly employed, whereas in post-partum thymocytes the Tcrd-V1 gene segment was preferentially used. These Tcrd-V3 transcripts contained only a single D element (Dδ3) and a limited random nucleotide insertion. The Dδ3 element was also present in Tcrd-V3-containing transcripts derived from peripheral blood γδ Tcr+ clones. These data suggest that a wave of Tcr γδ might exist early in human fetal development that preferentially use the Tcrd-V3 gene segment. Springer Online Journal Archives 1860-2002 Stoep, Nienke oth Krijger, Ronald oth Bruining, Jan oth Koning, Frits oth Elsen, Peter oth in Immunogenetics 1974 32(1990) vom: Mai, Seite 331-336 (DE-627)NLEJ188990437 (DE-600)1398344-1 1432-1211 nnns volume:32 year:1990 month:05 pages:331-336 extent:6 http://dx.doi.org/10.1007/BF00211647 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 32 1990 5 331-336 6 |
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(DE-627)NLEJ202686132 DE-627 ger DE-627 rakwb eng Analysis of early fetal T-cell receptor δ chain in humans 1990 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract In the mouse it has been found that a number of T-cell receptor (Tcr) gd phenotypes are generated during fetal thymic development. To examine whether such “waves” of Tcrgd phenotypes can be found in man, we studied the V-region usage and junctional diversity of the T-cell receptor δ chain in human fetal and post-partum thymocytes and peripheral blood T cells. Using the polymerase chain reaction (PCR)-amplification technique it was found that in fetal thymocytes of 15–17 weeks of gestation the Tcrd-V3 gene segment was mainly employed, whereas in post-partum thymocytes the Tcrd-V1 gene segment was preferentially used. These Tcrd-V3 transcripts contained only a single D element (Dδ3) and a limited random nucleotide insertion. The Dδ3 element was also present in Tcrd-V3-containing transcripts derived from peripheral blood γδ Tcr+ clones. These data suggest that a wave of Tcr γδ might exist early in human fetal development that preferentially use the Tcrd-V3 gene segment. Springer Online Journal Archives 1860-2002 Stoep, Nienke oth Krijger, Ronald oth Bruining, Jan oth Koning, Frits oth Elsen, Peter oth in Immunogenetics 1974 32(1990) vom: Mai, Seite 331-336 (DE-627)NLEJ188990437 (DE-600)1398344-1 1432-1211 nnns volume:32 year:1990 month:05 pages:331-336 extent:6 http://dx.doi.org/10.1007/BF00211647 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 32 1990 5 331-336 6 |
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in Immunogenetics 32(1990) vom: Mai, Seite 331-336 volume:32 year:1990 month:05 pages:331-336 extent:6 |
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Abstract In the mouse it has been found that a number of T-cell receptor (Tcr) gd phenotypes are generated during fetal thymic development. To examine whether such “waves” of Tcrgd phenotypes can be found in man, we studied the V-region usage and junctional diversity of the T-cell receptor δ chain in human fetal and post-partum thymocytes and peripheral blood T cells. Using the polymerase chain reaction (PCR)-amplification technique it was found that in fetal thymocytes of 15–17 weeks of gestation the Tcrd-V3 gene segment was mainly employed, whereas in post-partum thymocytes the Tcrd-V1 gene segment was preferentially used. These Tcrd-V3 transcripts contained only a single D element (Dδ3) and a limited random nucleotide insertion. The Dδ3 element was also present in Tcrd-V3-containing transcripts derived from peripheral blood γδ Tcr+ clones. These data suggest that a wave of Tcr γδ might exist early in human fetal development that preferentially use the Tcrd-V3 gene segment. |
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Abstract In the mouse it has been found that a number of T-cell receptor (Tcr) gd phenotypes are generated during fetal thymic development. To examine whether such “waves” of Tcrgd phenotypes can be found in man, we studied the V-region usage and junctional diversity of the T-cell receptor δ chain in human fetal and post-partum thymocytes and peripheral blood T cells. Using the polymerase chain reaction (PCR)-amplification technique it was found that in fetal thymocytes of 15–17 weeks of gestation the Tcrd-V3 gene segment was mainly employed, whereas in post-partum thymocytes the Tcrd-V1 gene segment was preferentially used. These Tcrd-V3 transcripts contained only a single D element (Dδ3) and a limited random nucleotide insertion. The Dδ3 element was also present in Tcrd-V3-containing transcripts derived from peripheral blood γδ Tcr+ clones. These data suggest that a wave of Tcr γδ might exist early in human fetal development that preferentially use the Tcrd-V3 gene segment. |
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Abstract In the mouse it has been found that a number of T-cell receptor (Tcr) gd phenotypes are generated during fetal thymic development. To examine whether such “waves” of Tcrgd phenotypes can be found in man, we studied the V-region usage and junctional diversity of the T-cell receptor δ chain in human fetal and post-partum thymocytes and peripheral blood T cells. Using the polymerase chain reaction (PCR)-amplification technique it was found that in fetal thymocytes of 15–17 weeks of gestation the Tcrd-V3 gene segment was mainly employed, whereas in post-partum thymocytes the Tcrd-V1 gene segment was preferentially used. These Tcrd-V3 transcripts contained only a single D element (Dδ3) and a limited random nucleotide insertion. The Dδ3 element was also present in Tcrd-V3-containing transcripts derived from peripheral blood γδ Tcr+ clones. These data suggest that a wave of Tcr γδ might exist early in human fetal development that preferentially use the Tcrd-V3 gene segment. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ202686132</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210706105317.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070528s1990 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ202686132</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Analysis of early fetal T-cell receptor δ chain in humans</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1990</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">6</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract In the mouse it has been found that a number of T-cell receptor (Tcr) gd phenotypes are generated during fetal thymic development. To examine whether such “waves” of Tcrgd phenotypes can be found in man, we studied the V-region usage and junctional diversity of the T-cell receptor δ chain in human fetal and post-partum thymocytes and peripheral blood T cells. Using the polymerase chain reaction (PCR)-amplification technique it was found that in fetal thymocytes of 15–17 weeks of gestation the Tcrd-V3 gene segment was mainly employed, whereas in post-partum thymocytes the Tcrd-V1 gene segment was preferentially used. These Tcrd-V3 transcripts contained only a single D element (Dδ3) and a limited random nucleotide insertion. The Dδ3 element was also present in Tcrd-V3-containing transcripts derived from peripheral blood γδ Tcr+ clones. These data suggest that a wave of Tcr γδ might exist early in human fetal development that preferentially use the Tcrd-V3 gene segment.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Springer Online Journal Archives 1860-2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Stoep, Nienke</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Krijger, Ronald</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Bruining, Jan</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Koning, Frits</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Elsen, Peter</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">Immunogenetics</subfield><subfield code="d">1974</subfield><subfield code="g">32(1990) vom: Mai, Seite 331-336</subfield><subfield code="w">(DE-627)NLEJ188990437</subfield><subfield code="w">(DE-600)1398344-1</subfield><subfield code="x">1432-1211</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:32</subfield><subfield code="g">year:1990</subfield><subfield code="g">month:05</subfield><subfield code="g">pages:331-336</subfield><subfield code="g">extent:6</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1007/BF00211647</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SOJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">32</subfield><subfield code="j">1990</subfield><subfield code="c">5</subfield><subfield code="h">331-336</subfield><subfield code="g">6</subfield></datafield></record></collection>
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