Use of a microbial fuel cell for the rapid enumeration of bacteria
Summary The detection of bacteria using a thionine mediated microbial fuel cell was examined. On addition of bacteria to the anode compartment of a fuel cell, a rapid increase in the current output was observed. Both the total change in the steady state current (ΔmA) and the initial rate of change o...
Ausführliche Beschreibung
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Englisch |
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1988 |
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6 |
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Springer Online Journal Archives 1860-2002 |
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Übergeordnetes Werk: |
in: Applied microbiology and biotechnology - 1975, 28(1988) vom: Jan., Seite 26-31 |
Übergeordnetes Werk: |
volume:28 ; year:1988 ; month:01 ; pages:26-31 ; extent:6 |
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520 | |a Summary The detection of bacteria using a thionine mediated microbial fuel cell was examined. On addition of bacteria to the anode compartment of a fuel cell, a rapid increase in the current output was observed. Both the total change in the steady state current (ΔmA) and the initial rate of change of current were proportional to the numbers of bacteria added. Regression analysis of plots of log10 ΔmA against log10 bacteria ml-1 (final concentration) upon the addition of E. coli K12, Lactococcus lactis, coliform sp. A1, Micrococcus sp. M3 but not Pseudomonas sp. P5 gave reasonable correlation coefficients. Determination of the rates of respiration and thionine reduction by E. coli indicated that the transfer of metabolic electrons from the bacteria to the mediator was reasonably efficient (approx. 50%). These results are discussed with respect to the potential application of this technique for the rapid estimation of the bacterial contamination of foods. | ||
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700 | 1 | |a Kroll, Rohan G. |4 oth | |
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(DE-627)NLEJ202706559 DE-627 ger DE-627 rakwb eng Use of a microbial fuel cell for the rapid enumeration of bacteria 1988 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary The detection of bacteria using a thionine mediated microbial fuel cell was examined. On addition of bacteria to the anode compartment of a fuel cell, a rapid increase in the current output was observed. Both the total change in the steady state current (ΔmA) and the initial rate of change of current were proportional to the numbers of bacteria added. Regression analysis of plots of log10 ΔmA against log10 bacteria ml-1 (final concentration) upon the addition of E. coli K12, Lactococcus lactis, coliform sp. A1, Micrococcus sp. M3 but not Pseudomonas sp. P5 gave reasonable correlation coefficients. Determination of the rates of respiration and thionine reduction by E. coli indicated that the transfer of metabolic electrons from the bacteria to the mediator was reasonably efficient (approx. 50%). These results are discussed with respect to the potential application of this technique for the rapid estimation of the bacterial contamination of foods. Springer Online Journal Archives 1860-2002 Patchett, Roy A. oth Kelly, Alison F. oth Kroll, Rohan G. oth in Applied microbiology and biotechnology 1975 28(1988) vom: Jan., Seite 26-31 (DE-627)NLEJ188988920 (DE-600)1464336-4 1432-0614 nnns volume:28 year:1988 month:01 pages:26-31 extent:6 http://dx.doi.org/10.1007/BF00250492 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 28 1988 1 26-31 6 |
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(DE-627)NLEJ202706559 DE-627 ger DE-627 rakwb eng Use of a microbial fuel cell for the rapid enumeration of bacteria 1988 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary The detection of bacteria using a thionine mediated microbial fuel cell was examined. On addition of bacteria to the anode compartment of a fuel cell, a rapid increase in the current output was observed. Both the total change in the steady state current (ΔmA) and the initial rate of change of current were proportional to the numbers of bacteria added. Regression analysis of plots of log10 ΔmA against log10 bacteria ml-1 (final concentration) upon the addition of E. coli K12, Lactococcus lactis, coliform sp. A1, Micrococcus sp. M3 but not Pseudomonas sp. P5 gave reasonable correlation coefficients. Determination of the rates of respiration and thionine reduction by E. coli indicated that the transfer of metabolic electrons from the bacteria to the mediator was reasonably efficient (approx. 50%). These results are discussed with respect to the potential application of this technique for the rapid estimation of the bacterial contamination of foods. Springer Online Journal Archives 1860-2002 Patchett, Roy A. oth Kelly, Alison F. oth Kroll, Rohan G. oth in Applied microbiology and biotechnology 1975 28(1988) vom: Jan., Seite 26-31 (DE-627)NLEJ188988920 (DE-600)1464336-4 1432-0614 nnns volume:28 year:1988 month:01 pages:26-31 extent:6 http://dx.doi.org/10.1007/BF00250492 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 28 1988 1 26-31 6 |
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(DE-627)NLEJ202706559 DE-627 ger DE-627 rakwb eng Use of a microbial fuel cell for the rapid enumeration of bacteria 1988 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary The detection of bacteria using a thionine mediated microbial fuel cell was examined. On addition of bacteria to the anode compartment of a fuel cell, a rapid increase in the current output was observed. Both the total change in the steady state current (ΔmA) and the initial rate of change of current were proportional to the numbers of bacteria added. Regression analysis of plots of log10 ΔmA against log10 bacteria ml-1 (final concentration) upon the addition of E. coli K12, Lactococcus lactis, coliform sp. A1, Micrococcus sp. M3 but not Pseudomonas sp. P5 gave reasonable correlation coefficients. Determination of the rates of respiration and thionine reduction by E. coli indicated that the transfer of metabolic electrons from the bacteria to the mediator was reasonably efficient (approx. 50%). These results are discussed with respect to the potential application of this technique for the rapid estimation of the bacterial contamination of foods. Springer Online Journal Archives 1860-2002 Patchett, Roy A. oth Kelly, Alison F. oth Kroll, Rohan G. oth in Applied microbiology and biotechnology 1975 28(1988) vom: Jan., Seite 26-31 (DE-627)NLEJ188988920 (DE-600)1464336-4 1432-0614 nnns volume:28 year:1988 month:01 pages:26-31 extent:6 http://dx.doi.org/10.1007/BF00250492 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 28 1988 1 26-31 6 |
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(DE-627)NLEJ202706559 DE-627 ger DE-627 rakwb eng Use of a microbial fuel cell for the rapid enumeration of bacteria 1988 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary The detection of bacteria using a thionine mediated microbial fuel cell was examined. On addition of bacteria to the anode compartment of a fuel cell, a rapid increase in the current output was observed. Both the total change in the steady state current (ΔmA) and the initial rate of change of current were proportional to the numbers of bacteria added. Regression analysis of plots of log10 ΔmA against log10 bacteria ml-1 (final concentration) upon the addition of E. coli K12, Lactococcus lactis, coliform sp. A1, Micrococcus sp. M3 but not Pseudomonas sp. P5 gave reasonable correlation coefficients. Determination of the rates of respiration and thionine reduction by E. coli indicated that the transfer of metabolic electrons from the bacteria to the mediator was reasonably efficient (approx. 50%). These results are discussed with respect to the potential application of this technique for the rapid estimation of the bacterial contamination of foods. Springer Online Journal Archives 1860-2002 Patchett, Roy A. oth Kelly, Alison F. oth Kroll, Rohan G. oth in Applied microbiology and biotechnology 1975 28(1988) vom: Jan., Seite 26-31 (DE-627)NLEJ188988920 (DE-600)1464336-4 1432-0614 nnns volume:28 year:1988 month:01 pages:26-31 extent:6 http://dx.doi.org/10.1007/BF00250492 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 28 1988 1 26-31 6 |
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(DE-627)NLEJ202706559 DE-627 ger DE-627 rakwb eng Use of a microbial fuel cell for the rapid enumeration of bacteria 1988 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary The detection of bacteria using a thionine mediated microbial fuel cell was examined. On addition of bacteria to the anode compartment of a fuel cell, a rapid increase in the current output was observed. Both the total change in the steady state current (ΔmA) and the initial rate of change of current were proportional to the numbers of bacteria added. Regression analysis of plots of log10 ΔmA against log10 bacteria ml-1 (final concentration) upon the addition of E. coli K12, Lactococcus lactis, coliform sp. A1, Micrococcus sp. M3 but not Pseudomonas sp. P5 gave reasonable correlation coefficients. Determination of the rates of respiration and thionine reduction by E. coli indicated that the transfer of metabolic electrons from the bacteria to the mediator was reasonably efficient (approx. 50%). These results are discussed with respect to the potential application of this technique for the rapid estimation of the bacterial contamination of foods. Springer Online Journal Archives 1860-2002 Patchett, Roy A. oth Kelly, Alison F. oth Kroll, Rohan G. oth in Applied microbiology and biotechnology 1975 28(1988) vom: Jan., Seite 26-31 (DE-627)NLEJ188988920 (DE-600)1464336-4 1432-0614 nnns volume:28 year:1988 month:01 pages:26-31 extent:6 http://dx.doi.org/10.1007/BF00250492 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 28 1988 1 26-31 6 |
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Use of a microbial fuel cell for the rapid enumeration of bacteria |
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Summary The detection of bacteria using a thionine mediated microbial fuel cell was examined. On addition of bacteria to the anode compartment of a fuel cell, a rapid increase in the current output was observed. Both the total change in the steady state current (ΔmA) and the initial rate of change of current were proportional to the numbers of bacteria added. Regression analysis of plots of log10 ΔmA against log10 bacteria ml-1 (final concentration) upon the addition of E. coli K12, Lactococcus lactis, coliform sp. A1, Micrococcus sp. M3 but not Pseudomonas sp. P5 gave reasonable correlation coefficients. Determination of the rates of respiration and thionine reduction by E. coli indicated that the transfer of metabolic electrons from the bacteria to the mediator was reasonably efficient (approx. 50%). These results are discussed with respect to the potential application of this technique for the rapid estimation of the bacterial contamination of foods. |
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Summary The detection of bacteria using a thionine mediated microbial fuel cell was examined. On addition of bacteria to the anode compartment of a fuel cell, a rapid increase in the current output was observed. Both the total change in the steady state current (ΔmA) and the initial rate of change of current were proportional to the numbers of bacteria added. Regression analysis of plots of log10 ΔmA against log10 bacteria ml-1 (final concentration) upon the addition of E. coli K12, Lactococcus lactis, coliform sp. A1, Micrococcus sp. M3 but not Pseudomonas sp. P5 gave reasonable correlation coefficients. Determination of the rates of respiration and thionine reduction by E. coli indicated that the transfer of metabolic electrons from the bacteria to the mediator was reasonably efficient (approx. 50%). These results are discussed with respect to the potential application of this technique for the rapid estimation of the bacterial contamination of foods. |
abstract_unstemmed |
Summary The detection of bacteria using a thionine mediated microbial fuel cell was examined. On addition of bacteria to the anode compartment of a fuel cell, a rapid increase in the current output was observed. Both the total change in the steady state current (ΔmA) and the initial rate of change of current were proportional to the numbers of bacteria added. Regression analysis of plots of log10 ΔmA against log10 bacteria ml-1 (final concentration) upon the addition of E. coli K12, Lactococcus lactis, coliform sp. A1, Micrococcus sp. M3 but not Pseudomonas sp. P5 gave reasonable correlation coefficients. Determination of the rates of respiration and thionine reduction by E. coli indicated that the transfer of metabolic electrons from the bacteria to the mediator was reasonably efficient (approx. 50%). These results are discussed with respect to the potential application of this technique for the rapid estimation of the bacterial contamination of foods. |
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