Molecular cloning and expression of a xylanase gene from Cellulomonas sp. into Escherichia coli
Summary The xylanase gene of Cellulomonas sp. NCIM 2353 was cloned in pUC 18 and selected by growth on xylan as the sole carbon source. The functional clone harboured the recombinant plasmid with an insert of 1.42 kbp, as determined by restriction mapping and Southern hydridization. The clone secret...
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1990 |
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| Umfang: |
6 |
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| Reproduktion: |
Springer Online Journal Archives 1860-2002 |
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| Übergeordnetes Werk: |
in: Applied microbiology and biotechnology - 1975, 34(1990) vom: Jan., Seite 71-76 |
| Übergeordnetes Werk: |
volume:34 ; year:1990 ; month:01 ; pages:71-76 ; extent:6 |
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NLEJ202728420 |
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| 245 | 1 | 0 | |a Molecular cloning and expression of a xylanase gene from Cellulomonas sp. into Escherichia coli |
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| 520 | |a Summary The xylanase gene of Cellulomonas sp. NCIM 2353 was cloned in pUC 18 and selected by growth on xylan as the sole carbon source. The functional clone harboured the recombinant plasmid with an insert of 1.42 kbp, as determined by restriction mapping and Southern hydridization. The clone secreted a xylanase of 45 000 mol. wt. as determined by Western blot analysis using specific antixylanase antibodies. The DNA insert carried the full structural gene along with its promoter and possibly regulatory sequences, since xylanase activity in the clone Cs11 was inducible by xylan. | ||
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| 700 | 1 | |a Patki, A. H. |4 oth | |
| 700 | 1 | |a Bhave, M. |4 oth | |
| 700 | 1 | |a Khurana, I. |4 oth | |
| 700 | 1 | |a Deobagkar, D. N. |4 oth | |
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(DE-627)NLEJ202728420 DE-627 ger DE-627 rakwb eng Molecular cloning and expression of a xylanase gene from Cellulomonas sp. into Escherichia coli 1990 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary The xylanase gene of Cellulomonas sp. NCIM 2353 was cloned in pUC 18 and selected by growth on xylan as the sole carbon source. The functional clone harboured the recombinant plasmid with an insert of 1.42 kbp, as determined by restriction mapping and Southern hydridization. The clone secreted a xylanase of 45 000 mol. wt. as determined by Western blot analysis using specific antixylanase antibodies. The DNA insert carried the full structural gene along with its promoter and possibly regulatory sequences, since xylanase activity in the clone Cs11 was inducible by xylan. Springer Online Journal Archives 1860-2002 Bhalerao, J. oth Patki, A. H. oth Bhave, M. oth Khurana, I. oth Deobagkar, D. N. oth in Applied microbiology and biotechnology 1975 34(1990) vom: Jan., Seite 71-76 (DE-627)NLEJ188988920 (DE-600)1464336-4 1432-0614 nnns volume:34 year:1990 month:01 pages:71-76 extent:6 http://dx.doi.org/10.1007/BF00170926 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 34 1990 1 71-76 6 |
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(DE-627)NLEJ202728420 DE-627 ger DE-627 rakwb eng Molecular cloning and expression of a xylanase gene from Cellulomonas sp. into Escherichia coli 1990 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary The xylanase gene of Cellulomonas sp. NCIM 2353 was cloned in pUC 18 and selected by growth on xylan as the sole carbon source. The functional clone harboured the recombinant plasmid with an insert of 1.42 kbp, as determined by restriction mapping and Southern hydridization. The clone secreted a xylanase of 45 000 mol. wt. as determined by Western blot analysis using specific antixylanase antibodies. The DNA insert carried the full structural gene along with its promoter and possibly regulatory sequences, since xylanase activity in the clone Cs11 was inducible by xylan. Springer Online Journal Archives 1860-2002 Bhalerao, J. oth Patki, A. H. oth Bhave, M. oth Khurana, I. oth Deobagkar, D. N. oth in Applied microbiology and biotechnology 1975 34(1990) vom: Jan., Seite 71-76 (DE-627)NLEJ188988920 (DE-600)1464336-4 1432-0614 nnns volume:34 year:1990 month:01 pages:71-76 extent:6 http://dx.doi.org/10.1007/BF00170926 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 34 1990 1 71-76 6 |
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(DE-627)NLEJ202728420 DE-627 ger DE-627 rakwb eng Molecular cloning and expression of a xylanase gene from Cellulomonas sp. into Escherichia coli 1990 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary The xylanase gene of Cellulomonas sp. NCIM 2353 was cloned in pUC 18 and selected by growth on xylan as the sole carbon source. The functional clone harboured the recombinant plasmid with an insert of 1.42 kbp, as determined by restriction mapping and Southern hydridization. The clone secreted a xylanase of 45 000 mol. wt. as determined by Western blot analysis using specific antixylanase antibodies. The DNA insert carried the full structural gene along with its promoter and possibly regulatory sequences, since xylanase activity in the clone Cs11 was inducible by xylan. Springer Online Journal Archives 1860-2002 Bhalerao, J. oth Patki, A. H. oth Bhave, M. oth Khurana, I. oth Deobagkar, D. N. oth in Applied microbiology and biotechnology 1975 34(1990) vom: Jan., Seite 71-76 (DE-627)NLEJ188988920 (DE-600)1464336-4 1432-0614 nnns volume:34 year:1990 month:01 pages:71-76 extent:6 http://dx.doi.org/10.1007/BF00170926 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 34 1990 1 71-76 6 |
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(DE-627)NLEJ202728420 DE-627 ger DE-627 rakwb eng Molecular cloning and expression of a xylanase gene from Cellulomonas sp. into Escherichia coli 1990 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary The xylanase gene of Cellulomonas sp. NCIM 2353 was cloned in pUC 18 and selected by growth on xylan as the sole carbon source. The functional clone harboured the recombinant plasmid with an insert of 1.42 kbp, as determined by restriction mapping and Southern hydridization. The clone secreted a xylanase of 45 000 mol. wt. as determined by Western blot analysis using specific antixylanase antibodies. The DNA insert carried the full structural gene along with its promoter and possibly regulatory sequences, since xylanase activity in the clone Cs11 was inducible by xylan. Springer Online Journal Archives 1860-2002 Bhalerao, J. oth Patki, A. H. oth Bhave, M. oth Khurana, I. oth Deobagkar, D. N. oth in Applied microbiology and biotechnology 1975 34(1990) vom: Jan., Seite 71-76 (DE-627)NLEJ188988920 (DE-600)1464336-4 1432-0614 nnns volume:34 year:1990 month:01 pages:71-76 extent:6 http://dx.doi.org/10.1007/BF00170926 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 34 1990 1 71-76 6 |
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(DE-627)NLEJ202728420 DE-627 ger DE-627 rakwb eng Molecular cloning and expression of a xylanase gene from Cellulomonas sp. into Escherichia coli 1990 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary The xylanase gene of Cellulomonas sp. NCIM 2353 was cloned in pUC 18 and selected by growth on xylan as the sole carbon source. The functional clone harboured the recombinant plasmid with an insert of 1.42 kbp, as determined by restriction mapping and Southern hydridization. The clone secreted a xylanase of 45 000 mol. wt. as determined by Western blot analysis using specific antixylanase antibodies. The DNA insert carried the full structural gene along with its promoter and possibly regulatory sequences, since xylanase activity in the clone Cs11 was inducible by xylan. Springer Online Journal Archives 1860-2002 Bhalerao, J. oth Patki, A. H. oth Bhave, M. oth Khurana, I. oth Deobagkar, D. N. oth in Applied microbiology and biotechnology 1975 34(1990) vom: Jan., Seite 71-76 (DE-627)NLEJ188988920 (DE-600)1464336-4 1432-0614 nnns volume:34 year:1990 month:01 pages:71-76 extent:6 http://dx.doi.org/10.1007/BF00170926 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 34 1990 1 71-76 6 |
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molecular cloning and expression of a xylanase gene from cellulomonas sp. into escherichia coli |
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Molecular cloning and expression of a xylanase gene from Cellulomonas sp. into Escherichia coli |
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Summary The xylanase gene of Cellulomonas sp. NCIM 2353 was cloned in pUC 18 and selected by growth on xylan as the sole carbon source. The functional clone harboured the recombinant plasmid with an insert of 1.42 kbp, as determined by restriction mapping and Southern hydridization. The clone secreted a xylanase of 45 000 mol. wt. as determined by Western blot analysis using specific antixylanase antibodies. The DNA insert carried the full structural gene along with its promoter and possibly regulatory sequences, since xylanase activity in the clone Cs11 was inducible by xylan. |
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Summary The xylanase gene of Cellulomonas sp. NCIM 2353 was cloned in pUC 18 and selected by growth on xylan as the sole carbon source. The functional clone harboured the recombinant plasmid with an insert of 1.42 kbp, as determined by restriction mapping and Southern hydridization. The clone secreted a xylanase of 45 000 mol. wt. as determined by Western blot analysis using specific antixylanase antibodies. The DNA insert carried the full structural gene along with its promoter and possibly regulatory sequences, since xylanase activity in the clone Cs11 was inducible by xylan. |
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Summary The xylanase gene of Cellulomonas sp. NCIM 2353 was cloned in pUC 18 and selected by growth on xylan as the sole carbon source. The functional clone harboured the recombinant plasmid with an insert of 1.42 kbp, as determined by restriction mapping and Southern hydridization. The clone secreted a xylanase of 45 000 mol. wt. as determined by Western blot analysis using specific antixylanase antibodies. The DNA insert carried the full structural gene along with its promoter and possibly regulatory sequences, since xylanase activity in the clone Cs11 was inducible by xylan. |
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Molecular cloning and expression of a xylanase gene from Cellulomonas sp. into Escherichia coli |
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