In vivo survival of rabbit platelets by rapid freezing and thawing
Conclusions Our studies showed that at very rapid cooling rates of more than 100° C. per second in liquid helium the platelets are damaged and after thawing do not circulate in the peripheral blood. We don't have any explanation for this. But it seems that ice crystals are not the only reason f...
Ausführliche Beschreibung
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| Autor*in: |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1969 |
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| Umfang: |
4 |
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| Reproduktion: |
Springer Online Journal Archives 1860-2002 |
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| Übergeordnetes Werk: |
in: Annals of hematology - 1955, 19(1969) vom: Juni, Seite 347-350 |
| Übergeordnetes Werk: |
volume:19 ; year:1969 ; month:06 ; pages:347-350 ; extent:4 |
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NLEJ202936473 |
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| 245 | 1 | 0 | |a In vivo survival of rabbit platelets by rapid freezing and thawing |
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| 520 | |a Conclusions Our studies showed that at very rapid cooling rates of more than 100° C. per second in liquid helium the platelets are damaged and after thawing do not circulate in the peripheral blood. We don't have any explanation for this. But it seems that ice crystals are not the only reason for cell damage. The experiments with different thawing rates indicate that approximately 45° C. per second is optimum. While glycerol in a concentration of 10 per cent was not suitable for preservation of rabbit platelets at the high freezing and thawing rates used, the results with DMAC were encouraging. Toxicity of DMAC for human platelets and for animals and man should be determined. In addition, it should be clarified whether human platelets behave similarly to those of the rabbit frozen in liquid nitrogen without protective agents. If platelets could be frozen without cryophylactic additives, all questions of toxicity both to the platelets and the recipient would be eliminated. | ||
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| 700 | 1 | |a Michlmayr, G. |4 oth | |
| 700 | 1 | |a Weber, F. |4 oth | |
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(DE-627)NLEJ202936473 DE-627 ger DE-627 rakwb eng In vivo survival of rabbit platelets by rapid freezing and thawing 1969 4 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Conclusions Our studies showed that at very rapid cooling rates of more than 100° C. per second in liquid helium the platelets are damaged and after thawing do not circulate in the peripheral blood. We don't have any explanation for this. But it seems that ice crystals are not the only reason for cell damage. The experiments with different thawing rates indicate that approximately 45° C. per second is optimum. While glycerol in a concentration of 10 per cent was not suitable for preservation of rabbit platelets at the high freezing and thawing rates used, the results with DMAC were encouraging. Toxicity of DMAC for human platelets and for animals and man should be determined. In addition, it should be clarified whether human platelets behave similarly to those of the rabbit frozen in liquid nitrogen without protective agents. If platelets could be frozen without cryophylactic additives, all questions of toxicity both to the platelets and the recipient would be eliminated. Springer Online Journal Archives 1860-2002 Pfisterer, H. oth Michlmayr, G. oth Weber, F. oth in Annals of hematology 1955 19(1969) vom: Juni, Seite 347-350 (DE-627)NLEJ18898836X (DE-600)1458429-3 1432-0584 nnns volume:19 year:1969 month:06 pages:347-350 extent:4 http://dx.doi.org/10.1007/BF01632893 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 19 1969 6 347-350 4 |
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(DE-627)NLEJ202936473 DE-627 ger DE-627 rakwb eng In vivo survival of rabbit platelets by rapid freezing and thawing 1969 4 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Conclusions Our studies showed that at very rapid cooling rates of more than 100° C. per second in liquid helium the platelets are damaged and after thawing do not circulate in the peripheral blood. We don't have any explanation for this. But it seems that ice crystals are not the only reason for cell damage. The experiments with different thawing rates indicate that approximately 45° C. per second is optimum. While glycerol in a concentration of 10 per cent was not suitable for preservation of rabbit platelets at the high freezing and thawing rates used, the results with DMAC were encouraging. Toxicity of DMAC for human platelets and for animals and man should be determined. In addition, it should be clarified whether human platelets behave similarly to those of the rabbit frozen in liquid nitrogen without protective agents. If platelets could be frozen without cryophylactic additives, all questions of toxicity both to the platelets and the recipient would be eliminated. Springer Online Journal Archives 1860-2002 Pfisterer, H. oth Michlmayr, G. oth Weber, F. oth in Annals of hematology 1955 19(1969) vom: Juni, Seite 347-350 (DE-627)NLEJ18898836X (DE-600)1458429-3 1432-0584 nnns volume:19 year:1969 month:06 pages:347-350 extent:4 http://dx.doi.org/10.1007/BF01632893 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 19 1969 6 347-350 4 |
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(DE-627)NLEJ202936473 DE-627 ger DE-627 rakwb eng In vivo survival of rabbit platelets by rapid freezing and thawing 1969 4 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Conclusions Our studies showed that at very rapid cooling rates of more than 100° C. per second in liquid helium the platelets are damaged and after thawing do not circulate in the peripheral blood. We don't have any explanation for this. But it seems that ice crystals are not the only reason for cell damage. The experiments with different thawing rates indicate that approximately 45° C. per second is optimum. While glycerol in a concentration of 10 per cent was not suitable for preservation of rabbit platelets at the high freezing and thawing rates used, the results with DMAC were encouraging. Toxicity of DMAC for human platelets and for animals and man should be determined. In addition, it should be clarified whether human platelets behave similarly to those of the rabbit frozen in liquid nitrogen without protective agents. If platelets could be frozen without cryophylactic additives, all questions of toxicity both to the platelets and the recipient would be eliminated. Springer Online Journal Archives 1860-2002 Pfisterer, H. oth Michlmayr, G. oth Weber, F. oth in Annals of hematology 1955 19(1969) vom: Juni, Seite 347-350 (DE-627)NLEJ18898836X (DE-600)1458429-3 1432-0584 nnns volume:19 year:1969 month:06 pages:347-350 extent:4 http://dx.doi.org/10.1007/BF01632893 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 19 1969 6 347-350 4 |
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(DE-627)NLEJ202936473 DE-627 ger DE-627 rakwb eng In vivo survival of rabbit platelets by rapid freezing and thawing 1969 4 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Conclusions Our studies showed that at very rapid cooling rates of more than 100° C. per second in liquid helium the platelets are damaged and after thawing do not circulate in the peripheral blood. We don't have any explanation for this. But it seems that ice crystals are not the only reason for cell damage. The experiments with different thawing rates indicate that approximately 45° C. per second is optimum. While glycerol in a concentration of 10 per cent was not suitable for preservation of rabbit platelets at the high freezing and thawing rates used, the results with DMAC were encouraging. Toxicity of DMAC for human platelets and for animals and man should be determined. In addition, it should be clarified whether human platelets behave similarly to those of the rabbit frozen in liquid nitrogen without protective agents. If platelets could be frozen without cryophylactic additives, all questions of toxicity both to the platelets and the recipient would be eliminated. Springer Online Journal Archives 1860-2002 Pfisterer, H. oth Michlmayr, G. oth Weber, F. oth in Annals of hematology 1955 19(1969) vom: Juni, Seite 347-350 (DE-627)NLEJ18898836X (DE-600)1458429-3 1432-0584 nnns volume:19 year:1969 month:06 pages:347-350 extent:4 http://dx.doi.org/10.1007/BF01632893 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 19 1969 6 347-350 4 |
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(DE-627)NLEJ202936473 DE-627 ger DE-627 rakwb eng In vivo survival of rabbit platelets by rapid freezing and thawing 1969 4 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Conclusions Our studies showed that at very rapid cooling rates of more than 100° C. per second in liquid helium the platelets are damaged and after thawing do not circulate in the peripheral blood. We don't have any explanation for this. But it seems that ice crystals are not the only reason for cell damage. The experiments with different thawing rates indicate that approximately 45° C. per second is optimum. While glycerol in a concentration of 10 per cent was not suitable for preservation of rabbit platelets at the high freezing and thawing rates used, the results with DMAC were encouraging. Toxicity of DMAC for human platelets and for animals and man should be determined. In addition, it should be clarified whether human platelets behave similarly to those of the rabbit frozen in liquid nitrogen without protective agents. If platelets could be frozen without cryophylactic additives, all questions of toxicity both to the platelets and the recipient would be eliminated. Springer Online Journal Archives 1860-2002 Pfisterer, H. oth Michlmayr, G. oth Weber, F. oth in Annals of hematology 1955 19(1969) vom: Juni, Seite 347-350 (DE-627)NLEJ18898836X (DE-600)1458429-3 1432-0584 nnns volume:19 year:1969 month:06 pages:347-350 extent:4 http://dx.doi.org/10.1007/BF01632893 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 19 1969 6 347-350 4 |
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in vivo survival of rabbit platelets by rapid freezing and thawing |
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In vivo survival of rabbit platelets by rapid freezing and thawing |
| abstract |
Conclusions Our studies showed that at very rapid cooling rates of more than 100° C. per second in liquid helium the platelets are damaged and after thawing do not circulate in the peripheral blood. We don't have any explanation for this. But it seems that ice crystals are not the only reason for cell damage. The experiments with different thawing rates indicate that approximately 45° C. per second is optimum. While glycerol in a concentration of 10 per cent was not suitable for preservation of rabbit platelets at the high freezing and thawing rates used, the results with DMAC were encouraging. Toxicity of DMAC for human platelets and for animals and man should be determined. In addition, it should be clarified whether human platelets behave similarly to those of the rabbit frozen in liquid nitrogen without protective agents. If platelets could be frozen without cryophylactic additives, all questions of toxicity both to the platelets and the recipient would be eliminated. |
| abstractGer |
Conclusions Our studies showed that at very rapid cooling rates of more than 100° C. per second in liquid helium the platelets are damaged and after thawing do not circulate in the peripheral blood. We don't have any explanation for this. But it seems that ice crystals are not the only reason for cell damage. The experiments with different thawing rates indicate that approximately 45° C. per second is optimum. While glycerol in a concentration of 10 per cent was not suitable for preservation of rabbit platelets at the high freezing and thawing rates used, the results with DMAC were encouraging. Toxicity of DMAC for human platelets and for animals and man should be determined. In addition, it should be clarified whether human platelets behave similarly to those of the rabbit frozen in liquid nitrogen without protective agents. If platelets could be frozen without cryophylactic additives, all questions of toxicity both to the platelets and the recipient would be eliminated. |
| abstract_unstemmed |
Conclusions Our studies showed that at very rapid cooling rates of more than 100° C. per second in liquid helium the platelets are damaged and after thawing do not circulate in the peripheral blood. We don't have any explanation for this. But it seems that ice crystals are not the only reason for cell damage. The experiments with different thawing rates indicate that approximately 45° C. per second is optimum. While glycerol in a concentration of 10 per cent was not suitable for preservation of rabbit platelets at the high freezing and thawing rates used, the results with DMAC were encouraging. Toxicity of DMAC for human platelets and for animals and man should be determined. In addition, it should be clarified whether human platelets behave similarly to those of the rabbit frozen in liquid nitrogen without protective agents. If platelets could be frozen without cryophylactic additives, all questions of toxicity both to the platelets and the recipient would be eliminated. |
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