Nuclear association in yeast of a hybrid vector containing mitochondrial DNA
Summary The hybrid vector pCP2, consisting of the bacterial plasmid pBR325, the nuclear gene Leu-2 of Saccharomyces cerevisiae and a fragment of mitochondrial DNA from Cephalosporium acremonium, was found to associate with the nucleus in a transformed strain of Saccharomyces cerevisiae. This was ind...
Ausführliche Beschreibung
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| Autor*in: |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1983 |
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| Umfang: |
2 |
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| Reproduktion: |
Springer Online Journal Archives 1860-2002 |
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| Übergeordnetes Werk: |
in: Current genetics - 1979, 7(1983) vom: Feb., Seite 165-166 |
| Übergeordnetes Werk: |
volume:7 ; year:1983 ; month:02 ; pages:165-166 ; extent:2 |
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NLEJ203135431 |
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| 245 | 1 | 0 | |a Nuclear association in yeast of a hybrid vector containing mitochondrial DNA |
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| 520 | |a Summary The hybrid vector pCP2, consisting of the bacterial plasmid pBR325, the nuclear gene Leu-2 of Saccharomyces cerevisiae and a fragment of mitochondrial DNA from Cephalosporium acremonium, was found to associate with the nucleus in a transformed strain of Saccharomyces cerevisiae. This was inducted by (1) efficient expression of the Leu-2 gene as evidenced by a short generation time on selective medium; (2) independence of Leu-2 gene expression from mitochondrial protein synthesis, since pCP2 was shown to replicate and to be expressed in petite mutants; (3) association of pCP2 with isolated DNA from nuclei as proved by transformation experiments with E. coli. | ||
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| 700 | 1 | |a Tudzynski, Paul |4 oth | |
| 700 | 1 | |a Esser, Karl |4 oth | |
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(DE-627)NLEJ203135431 DE-627 ger DE-627 rakwb eng Nuclear association in yeast of a hybrid vector containing mitochondrial DNA 1983 2 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary The hybrid vector pCP2, consisting of the bacterial plasmid pBR325, the nuclear gene Leu-2 of Saccharomyces cerevisiae and a fragment of mitochondrial DNA from Cephalosporium acremonium, was found to associate with the nucleus in a transformed strain of Saccharomyces cerevisiae. This was inducted by (1) efficient expression of the Leu-2 gene as evidenced by a short generation time on selective medium; (2) independence of Leu-2 gene expression from mitochondrial protein synthesis, since pCP2 was shown to replicate and to be expressed in petite mutants; (3) association of pCP2 with isolated DNA from nuclei as proved by transformation experiments with E. coli. Springer Online Journal Archives 1860-2002 Tudzynski, Paul oth Esser, Karl oth in Current genetics 1979 7(1983) vom: Feb., Seite 165-166 (DE-627)NLEJ18898433X (DE-600)1458984-9 1432-0983 nnns volume:7 year:1983 month:02 pages:165-166 extent:2 http://dx.doi.org/10.1007/BF00365643 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 7 1983 2 165-166 2 |
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(DE-627)NLEJ203135431 DE-627 ger DE-627 rakwb eng Nuclear association in yeast of a hybrid vector containing mitochondrial DNA 1983 2 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary The hybrid vector pCP2, consisting of the bacterial plasmid pBR325, the nuclear gene Leu-2 of Saccharomyces cerevisiae and a fragment of mitochondrial DNA from Cephalosporium acremonium, was found to associate with the nucleus in a transformed strain of Saccharomyces cerevisiae. This was inducted by (1) efficient expression of the Leu-2 gene as evidenced by a short generation time on selective medium; (2) independence of Leu-2 gene expression from mitochondrial protein synthesis, since pCP2 was shown to replicate and to be expressed in petite mutants; (3) association of pCP2 with isolated DNA from nuclei as proved by transformation experiments with E. coli. Springer Online Journal Archives 1860-2002 Tudzynski, Paul oth Esser, Karl oth in Current genetics 1979 7(1983) vom: Feb., Seite 165-166 (DE-627)NLEJ18898433X (DE-600)1458984-9 1432-0983 nnns volume:7 year:1983 month:02 pages:165-166 extent:2 http://dx.doi.org/10.1007/BF00365643 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 7 1983 2 165-166 2 |
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(DE-627)NLEJ203135431 DE-627 ger DE-627 rakwb eng Nuclear association in yeast of a hybrid vector containing mitochondrial DNA 1983 2 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary The hybrid vector pCP2, consisting of the bacterial plasmid pBR325, the nuclear gene Leu-2 of Saccharomyces cerevisiae and a fragment of mitochondrial DNA from Cephalosporium acremonium, was found to associate with the nucleus in a transformed strain of Saccharomyces cerevisiae. This was inducted by (1) efficient expression of the Leu-2 gene as evidenced by a short generation time on selective medium; (2) independence of Leu-2 gene expression from mitochondrial protein synthesis, since pCP2 was shown to replicate and to be expressed in petite mutants; (3) association of pCP2 with isolated DNA from nuclei as proved by transformation experiments with E. coli. Springer Online Journal Archives 1860-2002 Tudzynski, Paul oth Esser, Karl oth in Current genetics 1979 7(1983) vom: Feb., Seite 165-166 (DE-627)NLEJ18898433X (DE-600)1458984-9 1432-0983 nnns volume:7 year:1983 month:02 pages:165-166 extent:2 http://dx.doi.org/10.1007/BF00365643 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 7 1983 2 165-166 2 |
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(DE-627)NLEJ203135431 DE-627 ger DE-627 rakwb eng Nuclear association in yeast of a hybrid vector containing mitochondrial DNA 1983 2 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary The hybrid vector pCP2, consisting of the bacterial plasmid pBR325, the nuclear gene Leu-2 of Saccharomyces cerevisiae and a fragment of mitochondrial DNA from Cephalosporium acremonium, was found to associate with the nucleus in a transformed strain of Saccharomyces cerevisiae. This was inducted by (1) efficient expression of the Leu-2 gene as evidenced by a short generation time on selective medium; (2) independence of Leu-2 gene expression from mitochondrial protein synthesis, since pCP2 was shown to replicate and to be expressed in petite mutants; (3) association of pCP2 with isolated DNA from nuclei as proved by transformation experiments with E. coli. Springer Online Journal Archives 1860-2002 Tudzynski, Paul oth Esser, Karl oth in Current genetics 1979 7(1983) vom: Feb., Seite 165-166 (DE-627)NLEJ18898433X (DE-600)1458984-9 1432-0983 nnns volume:7 year:1983 month:02 pages:165-166 extent:2 http://dx.doi.org/10.1007/BF00365643 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 7 1983 2 165-166 2 |
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(DE-627)NLEJ203135431 DE-627 ger DE-627 rakwb eng Nuclear association in yeast of a hybrid vector containing mitochondrial DNA 1983 2 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary The hybrid vector pCP2, consisting of the bacterial plasmid pBR325, the nuclear gene Leu-2 of Saccharomyces cerevisiae and a fragment of mitochondrial DNA from Cephalosporium acremonium, was found to associate with the nucleus in a transformed strain of Saccharomyces cerevisiae. This was inducted by (1) efficient expression of the Leu-2 gene as evidenced by a short generation time on selective medium; (2) independence of Leu-2 gene expression from mitochondrial protein synthesis, since pCP2 was shown to replicate and to be expressed in petite mutants; (3) association of pCP2 with isolated DNA from nuclei as proved by transformation experiments with E. coli. Springer Online Journal Archives 1860-2002 Tudzynski, Paul oth Esser, Karl oth in Current genetics 1979 7(1983) vom: Feb., Seite 165-166 (DE-627)NLEJ18898433X (DE-600)1458984-9 1432-0983 nnns volume:7 year:1983 month:02 pages:165-166 extent:2 http://dx.doi.org/10.1007/BF00365643 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 7 1983 2 165-166 2 |
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nuclear association in yeast of a hybrid vector containing mitochondrial dna |
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Nuclear association in yeast of a hybrid vector containing mitochondrial DNA |
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Summary The hybrid vector pCP2, consisting of the bacterial plasmid pBR325, the nuclear gene Leu-2 of Saccharomyces cerevisiae and a fragment of mitochondrial DNA from Cephalosporium acremonium, was found to associate with the nucleus in a transformed strain of Saccharomyces cerevisiae. This was inducted by (1) efficient expression of the Leu-2 gene as evidenced by a short generation time on selective medium; (2) independence of Leu-2 gene expression from mitochondrial protein synthesis, since pCP2 was shown to replicate and to be expressed in petite mutants; (3) association of pCP2 with isolated DNA from nuclei as proved by transformation experiments with E. coli. |
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Summary The hybrid vector pCP2, consisting of the bacterial plasmid pBR325, the nuclear gene Leu-2 of Saccharomyces cerevisiae and a fragment of mitochondrial DNA from Cephalosporium acremonium, was found to associate with the nucleus in a transformed strain of Saccharomyces cerevisiae. This was inducted by (1) efficient expression of the Leu-2 gene as evidenced by a short generation time on selective medium; (2) independence of Leu-2 gene expression from mitochondrial protein synthesis, since pCP2 was shown to replicate and to be expressed in petite mutants; (3) association of pCP2 with isolated DNA from nuclei as proved by transformation experiments with E. coli. |
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Summary The hybrid vector pCP2, consisting of the bacterial plasmid pBR325, the nuclear gene Leu-2 of Saccharomyces cerevisiae and a fragment of mitochondrial DNA from Cephalosporium acremonium, was found to associate with the nucleus in a transformed strain of Saccharomyces cerevisiae. This was inducted by (1) efficient expression of the Leu-2 gene as evidenced by a short generation time on selective medium; (2) independence of Leu-2 gene expression from mitochondrial protein synthesis, since pCP2 was shown to replicate and to be expressed in petite mutants; (3) association of pCP2 with isolated DNA from nuclei as proved by transformation experiments with E. coli. |
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