Analysis of a set of RAPD markers by hybridization and sequencing in Brassica: a note of caution
Abstract A series of RAPD markers generated by a single 10-mer primer were analyzed by hybridization to amplified and genomic DNA and by sequencing in two Brassica species. Primer B18 produced different profiles of nine major bands each in both Brassica nigra (B genome) and B. napus (AC genomes). Cl...
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1995 |
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| Umfang: |
5 |
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| Reproduktion: |
Springer Online Journal Archives 1860-2002 |
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| Übergeordnetes Werk: |
in: Plant cell reports - 1981, 14(1995) vom: Okt., Seite 630-634 |
| Übergeordnetes Werk: |
volume:14 ; year:1995 ; month:10 ; pages:630-634 ; extent:5 |
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| 520 | |a Abstract A series of RAPD markers generated by a single 10-mer primer were analyzed by hybridization to amplified and genomic DNA and by sequencing in two Brassica species. Primer B18 produced different profiles of nine major bands each in both Brassica nigra (B genome) and B. napus (AC genomes). Cloning and sequencing of five B18 B. nigra amplification products revealed that they were all unrelated to each other. Only limited stretches of high similarity of up to 69 nucleotides were shared by some of these clones. Hybridization to genomic DNA indicated that only two corresponded to a highly repeated sequence, whereas the rest were low copy sequences. In spite of their lack of homology, when these clones were used as probes to amplified B. nigra DNA, they hybridized to multiple bands in the profile. Hybridization of B. nigra clones for bands of similar sizes in both species, failed to hybridize in B. napus, revealing lack of homology between the DNAs of the two species. Because of these inconsistencies, it is concluded that RAPD markers, although useful for genetical studies, should be used with caution specially when basing homology on cross-hybridization and fragment sizes. | ||
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(DE-627)NLEJ203154010 DE-627 ger DE-627 rakwb eng Analysis of a set of RAPD markers by hybridization and sequencing in Brassica: a note of caution 1995 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract A series of RAPD markers generated by a single 10-mer primer were analyzed by hybridization to amplified and genomic DNA and by sequencing in two Brassica species. Primer B18 produced different profiles of nine major bands each in both Brassica nigra (B genome) and B. napus (AC genomes). Cloning and sequencing of five B18 B. nigra amplification products revealed that they were all unrelated to each other. Only limited stretches of high similarity of up to 69 nucleotides were shared by some of these clones. Hybridization to genomic DNA indicated that only two corresponded to a highly repeated sequence, whereas the rest were low copy sequences. In spite of their lack of homology, when these clones were used as probes to amplified B. nigra DNA, they hybridized to multiple bands in the profile. Hybridization of B. nigra clones for bands of similar sizes in both species, failed to hybridize in B. napus, revealing lack of homology between the DNAs of the two species. Because of these inconsistencies, it is concluded that RAPD markers, although useful for genetical studies, should be used with caution specially when basing homology on cross-hybridization and fragment sizes. Springer Online Journal Archives 1860-2002 Quiros, Carlos F. oth This, Patrice oth Laudie, Michelle oth Benet, Ariadna oth Chevre, Anne-Marie oth Delseny, Michel oth in Plant cell reports 1981 14(1995) vom: Okt., Seite 630-634 (DE-627)NLEJ188984984 (DE-600)1462082-0 1432-203X nnns volume:14 year:1995 month:10 pages:630-634 extent:5 http://dx.doi.org/10.1007/BF00232727 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 14 1995 10 630-634 5 |
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(DE-627)NLEJ203154010 DE-627 ger DE-627 rakwb eng Analysis of a set of RAPD markers by hybridization and sequencing in Brassica: a note of caution 1995 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract A series of RAPD markers generated by a single 10-mer primer were analyzed by hybridization to amplified and genomic DNA and by sequencing in two Brassica species. Primer B18 produced different profiles of nine major bands each in both Brassica nigra (B genome) and B. napus (AC genomes). Cloning and sequencing of five B18 B. nigra amplification products revealed that they were all unrelated to each other. Only limited stretches of high similarity of up to 69 nucleotides were shared by some of these clones. Hybridization to genomic DNA indicated that only two corresponded to a highly repeated sequence, whereas the rest were low copy sequences. In spite of their lack of homology, when these clones were used as probes to amplified B. nigra DNA, they hybridized to multiple bands in the profile. Hybridization of B. nigra clones for bands of similar sizes in both species, failed to hybridize in B. napus, revealing lack of homology between the DNAs of the two species. Because of these inconsistencies, it is concluded that RAPD markers, although useful for genetical studies, should be used with caution specially when basing homology on cross-hybridization and fragment sizes. Springer Online Journal Archives 1860-2002 Quiros, Carlos F. oth This, Patrice oth Laudie, Michelle oth Benet, Ariadna oth Chevre, Anne-Marie oth Delseny, Michel oth in Plant cell reports 1981 14(1995) vom: Okt., Seite 630-634 (DE-627)NLEJ188984984 (DE-600)1462082-0 1432-203X nnns volume:14 year:1995 month:10 pages:630-634 extent:5 http://dx.doi.org/10.1007/BF00232727 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 14 1995 10 630-634 5 |
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(DE-627)NLEJ203154010 DE-627 ger DE-627 rakwb eng Analysis of a set of RAPD markers by hybridization and sequencing in Brassica: a note of caution 1995 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract A series of RAPD markers generated by a single 10-mer primer were analyzed by hybridization to amplified and genomic DNA and by sequencing in two Brassica species. Primer B18 produced different profiles of nine major bands each in both Brassica nigra (B genome) and B. napus (AC genomes). Cloning and sequencing of five B18 B. nigra amplification products revealed that they were all unrelated to each other. Only limited stretches of high similarity of up to 69 nucleotides were shared by some of these clones. Hybridization to genomic DNA indicated that only two corresponded to a highly repeated sequence, whereas the rest were low copy sequences. In spite of their lack of homology, when these clones were used as probes to amplified B. nigra DNA, they hybridized to multiple bands in the profile. Hybridization of B. nigra clones for bands of similar sizes in both species, failed to hybridize in B. napus, revealing lack of homology between the DNAs of the two species. Because of these inconsistencies, it is concluded that RAPD markers, although useful for genetical studies, should be used with caution specially when basing homology on cross-hybridization and fragment sizes. Springer Online Journal Archives 1860-2002 Quiros, Carlos F. oth This, Patrice oth Laudie, Michelle oth Benet, Ariadna oth Chevre, Anne-Marie oth Delseny, Michel oth in Plant cell reports 1981 14(1995) vom: Okt., Seite 630-634 (DE-627)NLEJ188984984 (DE-600)1462082-0 1432-203X nnns volume:14 year:1995 month:10 pages:630-634 extent:5 http://dx.doi.org/10.1007/BF00232727 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 14 1995 10 630-634 5 |
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(DE-627)NLEJ203154010 DE-627 ger DE-627 rakwb eng Analysis of a set of RAPD markers by hybridization and sequencing in Brassica: a note of caution 1995 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract A series of RAPD markers generated by a single 10-mer primer were analyzed by hybridization to amplified and genomic DNA and by sequencing in two Brassica species. Primer B18 produced different profiles of nine major bands each in both Brassica nigra (B genome) and B. napus (AC genomes). Cloning and sequencing of five B18 B. nigra amplification products revealed that they were all unrelated to each other. Only limited stretches of high similarity of up to 69 nucleotides were shared by some of these clones. Hybridization to genomic DNA indicated that only two corresponded to a highly repeated sequence, whereas the rest were low copy sequences. In spite of their lack of homology, when these clones were used as probes to amplified B. nigra DNA, they hybridized to multiple bands in the profile. Hybridization of B. nigra clones for bands of similar sizes in both species, failed to hybridize in B. napus, revealing lack of homology between the DNAs of the two species. Because of these inconsistencies, it is concluded that RAPD markers, although useful for genetical studies, should be used with caution specially when basing homology on cross-hybridization and fragment sizes. Springer Online Journal Archives 1860-2002 Quiros, Carlos F. oth This, Patrice oth Laudie, Michelle oth Benet, Ariadna oth Chevre, Anne-Marie oth Delseny, Michel oth in Plant cell reports 1981 14(1995) vom: Okt., Seite 630-634 (DE-627)NLEJ188984984 (DE-600)1462082-0 1432-203X nnns volume:14 year:1995 month:10 pages:630-634 extent:5 http://dx.doi.org/10.1007/BF00232727 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 14 1995 10 630-634 5 |
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(DE-627)NLEJ203154010 DE-627 ger DE-627 rakwb eng Analysis of a set of RAPD markers by hybridization and sequencing in Brassica: a note of caution 1995 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract A series of RAPD markers generated by a single 10-mer primer were analyzed by hybridization to amplified and genomic DNA and by sequencing in two Brassica species. Primer B18 produced different profiles of nine major bands each in both Brassica nigra (B genome) and B. napus (AC genomes). Cloning and sequencing of five B18 B. nigra amplification products revealed that they were all unrelated to each other. Only limited stretches of high similarity of up to 69 nucleotides were shared by some of these clones. Hybridization to genomic DNA indicated that only two corresponded to a highly repeated sequence, whereas the rest were low copy sequences. In spite of their lack of homology, when these clones were used as probes to amplified B. nigra DNA, they hybridized to multiple bands in the profile. Hybridization of B. nigra clones for bands of similar sizes in both species, failed to hybridize in B. napus, revealing lack of homology between the DNAs of the two species. Because of these inconsistencies, it is concluded that RAPD markers, although useful for genetical studies, should be used with caution specially when basing homology on cross-hybridization and fragment sizes. Springer Online Journal Archives 1860-2002 Quiros, Carlos F. oth This, Patrice oth Laudie, Michelle oth Benet, Ariadna oth Chevre, Anne-Marie oth Delseny, Michel oth in Plant cell reports 1981 14(1995) vom: Okt., Seite 630-634 (DE-627)NLEJ188984984 (DE-600)1462082-0 1432-203X nnns volume:14 year:1995 month:10 pages:630-634 extent:5 http://dx.doi.org/10.1007/BF00232727 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 14 1995 10 630-634 5 |
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Analysis of a set of RAPD markers by hybridization and sequencing in Brassica: a note of caution |
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Abstract A series of RAPD markers generated by a single 10-mer primer were analyzed by hybridization to amplified and genomic DNA and by sequencing in two Brassica species. Primer B18 produced different profiles of nine major bands each in both Brassica nigra (B genome) and B. napus (AC genomes). Cloning and sequencing of five B18 B. nigra amplification products revealed that they were all unrelated to each other. Only limited stretches of high similarity of up to 69 nucleotides were shared by some of these clones. Hybridization to genomic DNA indicated that only two corresponded to a highly repeated sequence, whereas the rest were low copy sequences. In spite of their lack of homology, when these clones were used as probes to amplified B. nigra DNA, they hybridized to multiple bands in the profile. Hybridization of B. nigra clones for bands of similar sizes in both species, failed to hybridize in B. napus, revealing lack of homology between the DNAs of the two species. Because of these inconsistencies, it is concluded that RAPD markers, although useful for genetical studies, should be used with caution specially when basing homology on cross-hybridization and fragment sizes. |
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Abstract A series of RAPD markers generated by a single 10-mer primer were analyzed by hybridization to amplified and genomic DNA and by sequencing in two Brassica species. Primer B18 produced different profiles of nine major bands each in both Brassica nigra (B genome) and B. napus (AC genomes). Cloning and sequencing of five B18 B. nigra amplification products revealed that they were all unrelated to each other. Only limited stretches of high similarity of up to 69 nucleotides were shared by some of these clones. Hybridization to genomic DNA indicated that only two corresponded to a highly repeated sequence, whereas the rest were low copy sequences. In spite of their lack of homology, when these clones were used as probes to amplified B. nigra DNA, they hybridized to multiple bands in the profile. Hybridization of B. nigra clones for bands of similar sizes in both species, failed to hybridize in B. napus, revealing lack of homology between the DNAs of the two species. Because of these inconsistencies, it is concluded that RAPD markers, although useful for genetical studies, should be used with caution specially when basing homology on cross-hybridization and fragment sizes. |
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Abstract A series of RAPD markers generated by a single 10-mer primer were analyzed by hybridization to amplified and genomic DNA and by sequencing in two Brassica species. Primer B18 produced different profiles of nine major bands each in both Brassica nigra (B genome) and B. napus (AC genomes). Cloning and sequencing of five B18 B. nigra amplification products revealed that they were all unrelated to each other. Only limited stretches of high similarity of up to 69 nucleotides were shared by some of these clones. Hybridization to genomic DNA indicated that only two corresponded to a highly repeated sequence, whereas the rest were low copy sequences. In spite of their lack of homology, when these clones were used as probes to amplified B. nigra DNA, they hybridized to multiple bands in the profile. Hybridization of B. nigra clones for bands of similar sizes in both species, failed to hybridize in B. napus, revealing lack of homology between the DNAs of the two species. Because of these inconsistencies, it is concluded that RAPD markers, although useful for genetical studies, should be used with caution specially when basing homology on cross-hybridization and fragment sizes. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ203154010</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210706120930.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070528s1995 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ203154010</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Analysis of a set of RAPD markers by hybridization and sequencing in Brassica: a note of caution</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1995</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">5</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract A series of RAPD markers generated by a single 10-mer primer were analyzed by hybridization to amplified and genomic DNA and by sequencing in two Brassica species. Primer B18 produced different profiles of nine major bands each in both Brassica nigra (B genome) and B. napus (AC genomes). Cloning and sequencing of five B18 B. nigra amplification products revealed that they were all unrelated to each other. Only limited stretches of high similarity of up to 69 nucleotides were shared by some of these clones. Hybridization to genomic DNA indicated that only two corresponded to a highly repeated sequence, whereas the rest were low copy sequences. In spite of their lack of homology, when these clones were used as probes to amplified B. nigra DNA, they hybridized to multiple bands in the profile. Hybridization of B. nigra clones for bands of similar sizes in both species, failed to hybridize in B. napus, revealing lack of homology between the DNAs of the two species. Because of these inconsistencies, it is concluded that RAPD markers, although useful for genetical studies, should be used with caution specially when basing homology on cross-hybridization and fragment sizes.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Springer Online Journal Archives 1860-2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Quiros, Carlos F.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">This, Patrice</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Laudie, Michelle</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Benet, Ariadna</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Chevre, Anne-Marie</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Delseny, Michel</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">Plant cell reports</subfield><subfield code="d">1981</subfield><subfield code="g">14(1995) vom: Okt., Seite 630-634</subfield><subfield code="w">(DE-627)NLEJ188984984</subfield><subfield code="w">(DE-600)1462082-0</subfield><subfield code="x">1432-203X</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:14</subfield><subfield code="g">year:1995</subfield><subfield code="g">month:10</subfield><subfield code="g">pages:630-634</subfield><subfield code="g">extent:5</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1007/BF00232727</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SOJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">14</subfield><subfield code="j">1995</subfield><subfield code="c">10</subfield><subfield code="h">630-634</subfield><subfield code="g">5</subfield></datafield></record></collection>
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