Effect of media components on the gynogenic regeneration of onion (Allium cepa L.) cultivars and analysis of regenerants
Summary Two separate experiments were performed to analyze the effects of different media on gynogenic regeneration in four onion cultivars. In a two step flower/ovary culture procedure, 2,4-dichlorophenoxyacetic acid added to the induction medium was superior to phenylacetic acid in the highly rege...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
|---|
| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
1996 |
|---|
| Umfang: |
5 |
|---|
| Reproduktion: |
Springer Online Journal Archives 1860-2002 |
|---|---|
| Übergeordnetes Werk: |
in: Plant cell reports - 1981, 15(1996) vom: Dez., Seite 934-938 |
| Übergeordnetes Werk: |
volume:15 ; year:1996 ; month:12 ; pages:934-938 ; extent:5 |
| Links: |
|---|
| Katalog-ID: |
NLEJ203156897 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLEJ203156897 | ||
| 003 | DE-627 | ||
| 005 | 20210706120956.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 070528s1996 xx |||||o 00| ||eng c | ||
| 035 | |a (DE-627)NLEJ203156897 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 245 | 1 | 0 | |a Effect of media components on the gynogenic regeneration of onion (Allium cepa L.) cultivars and analysis of regenerants |
| 264 | 1 | |c 1996 | |
| 300 | |a 5 | ||
| 336 | |a nicht spezifiziert |b zzz |2 rdacontent | ||
| 337 | |a nicht spezifiziert |b z |2 rdamedia | ||
| 338 | |a nicht spezifiziert |b zu |2 rdacarrier | ||
| 520 | |a Summary Two separate experiments were performed to analyze the effects of different media on gynogenic regeneration in four onion cultivars. In a two step flower/ovary culture procedure, 2,4-dichlorophenoxyacetic acid added to the induction medium was superior to phenylacetic acid in the highly regenerating cultivar, while the effect of thidiazuron in the regeneration medium was generally optimal in higher (2 mg/l) rather than in lower (0.2 mg/l) concentrations. Gellan-gum was compared to agar solidified media. A higher number of regenerants was achieved on the former, but an undesirable hyperhydricity of regenerants formed on gellan-gum solidified media greatly reduced the final survival of formed embryos. Analysis of the time interval needed for regeneration showed high variability (from 46 to 152 days after inoculation), which was particularly pronounced in genotypes with lower regeneration capacity. Simpler isozyme patterns of regenerants showed that all analysed regenerants of the cultivar with a high regeneration capacity were homozygous, while in the other three cultivars, a considerable percentage (11.1 to 36.4%) of heterozygous regenerants were also detected. Ploidy analysis of the regenerants with simpler isozyme patterns revealed that the majority of lines remain haploid. Identification of 2 homozygous triploid regenerants demonstrated that as in androgenesis, nuclear fusions can occur during gynogenic haploid regeneration. | ||
| 533 | |f Springer Online Journal Archives 1860-2002 | ||
| 700 | 1 | |a Jakše, M. |4 oth | |
| 700 | 1 | |a Bohanec, B. |4 oth | |
| 700 | 1 | |a Ihan, A. |4 oth | |
| 773 | 0 | 8 | |i in |t Plant cell reports |d 1981 |g 15(1996) vom: Dez., Seite 934-938 |w (DE-627)NLEJ188984984 |w (DE-600)1462082-0 |x 1432-203X |7 nnns |
| 773 | 1 | 8 | |g volume:15 |g year:1996 |g month:12 |g pages:934-938 |g extent:5 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1007/BF00231591 |
| 912 | |a GBV_USEFLAG_U | ||
| 912 | |a ZDB-1-SOJ | ||
| 912 | |a GBV_NL_ARTICLE | ||
| 951 | |a AR | ||
| 952 | |d 15 |j 1996 |c 12 |h 934-938 |g 5 | ||
| matchkey_str |
article:1432203X:1996----::fetfeicmoetoteyoeirgnrtoooinlimeaclia |
|---|---|
| hierarchy_sort_str |
1996 |
| publishDate |
1996 |
| allfields |
(DE-627)NLEJ203156897 DE-627 ger DE-627 rakwb eng Effect of media components on the gynogenic regeneration of onion (Allium cepa L.) cultivars and analysis of regenerants 1996 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary Two separate experiments were performed to analyze the effects of different media on gynogenic regeneration in four onion cultivars. In a two step flower/ovary culture procedure, 2,4-dichlorophenoxyacetic acid added to the induction medium was superior to phenylacetic acid in the highly regenerating cultivar, while the effect of thidiazuron in the regeneration medium was generally optimal in higher (2 mg/l) rather than in lower (0.2 mg/l) concentrations. Gellan-gum was compared to agar solidified media. A higher number of regenerants was achieved on the former, but an undesirable hyperhydricity of regenerants formed on gellan-gum solidified media greatly reduced the final survival of formed embryos. Analysis of the time interval needed for regeneration showed high variability (from 46 to 152 days after inoculation), which was particularly pronounced in genotypes with lower regeneration capacity. Simpler isozyme patterns of regenerants showed that all analysed regenerants of the cultivar with a high regeneration capacity were homozygous, while in the other three cultivars, a considerable percentage (11.1 to 36.4%) of heterozygous regenerants were also detected. Ploidy analysis of the regenerants with simpler isozyme patterns revealed that the majority of lines remain haploid. Identification of 2 homozygous triploid regenerants demonstrated that as in androgenesis, nuclear fusions can occur during gynogenic haploid regeneration. Springer Online Journal Archives 1860-2002 Jakše, M. oth Bohanec, B. oth Ihan, A. oth in Plant cell reports 1981 15(1996) vom: Dez., Seite 934-938 (DE-627)NLEJ188984984 (DE-600)1462082-0 1432-203X nnns volume:15 year:1996 month:12 pages:934-938 extent:5 http://dx.doi.org/10.1007/BF00231591 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 15 1996 12 934-938 5 |
| spelling |
(DE-627)NLEJ203156897 DE-627 ger DE-627 rakwb eng Effect of media components on the gynogenic regeneration of onion (Allium cepa L.) cultivars and analysis of regenerants 1996 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary Two separate experiments were performed to analyze the effects of different media on gynogenic regeneration in four onion cultivars. In a two step flower/ovary culture procedure, 2,4-dichlorophenoxyacetic acid added to the induction medium was superior to phenylacetic acid in the highly regenerating cultivar, while the effect of thidiazuron in the regeneration medium was generally optimal in higher (2 mg/l) rather than in lower (0.2 mg/l) concentrations. Gellan-gum was compared to agar solidified media. A higher number of regenerants was achieved on the former, but an undesirable hyperhydricity of regenerants formed on gellan-gum solidified media greatly reduced the final survival of formed embryos. Analysis of the time interval needed for regeneration showed high variability (from 46 to 152 days after inoculation), which was particularly pronounced in genotypes with lower regeneration capacity. Simpler isozyme patterns of regenerants showed that all analysed regenerants of the cultivar with a high regeneration capacity were homozygous, while in the other three cultivars, a considerable percentage (11.1 to 36.4%) of heterozygous regenerants were also detected. Ploidy analysis of the regenerants with simpler isozyme patterns revealed that the majority of lines remain haploid. Identification of 2 homozygous triploid regenerants demonstrated that as in androgenesis, nuclear fusions can occur during gynogenic haploid regeneration. Springer Online Journal Archives 1860-2002 Jakše, M. oth Bohanec, B. oth Ihan, A. oth in Plant cell reports 1981 15(1996) vom: Dez., Seite 934-938 (DE-627)NLEJ188984984 (DE-600)1462082-0 1432-203X nnns volume:15 year:1996 month:12 pages:934-938 extent:5 http://dx.doi.org/10.1007/BF00231591 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 15 1996 12 934-938 5 |
| allfields_unstemmed |
(DE-627)NLEJ203156897 DE-627 ger DE-627 rakwb eng Effect of media components on the gynogenic regeneration of onion (Allium cepa L.) cultivars and analysis of regenerants 1996 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary Two separate experiments were performed to analyze the effects of different media on gynogenic regeneration in four onion cultivars. In a two step flower/ovary culture procedure, 2,4-dichlorophenoxyacetic acid added to the induction medium was superior to phenylacetic acid in the highly regenerating cultivar, while the effect of thidiazuron in the regeneration medium was generally optimal in higher (2 mg/l) rather than in lower (0.2 mg/l) concentrations. Gellan-gum was compared to agar solidified media. A higher number of regenerants was achieved on the former, but an undesirable hyperhydricity of regenerants formed on gellan-gum solidified media greatly reduced the final survival of formed embryos. Analysis of the time interval needed for regeneration showed high variability (from 46 to 152 days after inoculation), which was particularly pronounced in genotypes with lower regeneration capacity. Simpler isozyme patterns of regenerants showed that all analysed regenerants of the cultivar with a high regeneration capacity were homozygous, while in the other three cultivars, a considerable percentage (11.1 to 36.4%) of heterozygous regenerants were also detected. Ploidy analysis of the regenerants with simpler isozyme patterns revealed that the majority of lines remain haploid. Identification of 2 homozygous triploid regenerants demonstrated that as in androgenesis, nuclear fusions can occur during gynogenic haploid regeneration. Springer Online Journal Archives 1860-2002 Jakše, M. oth Bohanec, B. oth Ihan, A. oth in Plant cell reports 1981 15(1996) vom: Dez., Seite 934-938 (DE-627)NLEJ188984984 (DE-600)1462082-0 1432-203X nnns volume:15 year:1996 month:12 pages:934-938 extent:5 http://dx.doi.org/10.1007/BF00231591 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 15 1996 12 934-938 5 |
| allfieldsGer |
(DE-627)NLEJ203156897 DE-627 ger DE-627 rakwb eng Effect of media components on the gynogenic regeneration of onion (Allium cepa L.) cultivars and analysis of regenerants 1996 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary Two separate experiments were performed to analyze the effects of different media on gynogenic regeneration in four onion cultivars. In a two step flower/ovary culture procedure, 2,4-dichlorophenoxyacetic acid added to the induction medium was superior to phenylacetic acid in the highly regenerating cultivar, while the effect of thidiazuron in the regeneration medium was generally optimal in higher (2 mg/l) rather than in lower (0.2 mg/l) concentrations. Gellan-gum was compared to agar solidified media. A higher number of regenerants was achieved on the former, but an undesirable hyperhydricity of regenerants formed on gellan-gum solidified media greatly reduced the final survival of formed embryos. Analysis of the time interval needed for regeneration showed high variability (from 46 to 152 days after inoculation), which was particularly pronounced in genotypes with lower regeneration capacity. Simpler isozyme patterns of regenerants showed that all analysed regenerants of the cultivar with a high regeneration capacity were homozygous, while in the other three cultivars, a considerable percentage (11.1 to 36.4%) of heterozygous regenerants were also detected. Ploidy analysis of the regenerants with simpler isozyme patterns revealed that the majority of lines remain haploid. Identification of 2 homozygous triploid regenerants demonstrated that as in androgenesis, nuclear fusions can occur during gynogenic haploid regeneration. Springer Online Journal Archives 1860-2002 Jakše, M. oth Bohanec, B. oth Ihan, A. oth in Plant cell reports 1981 15(1996) vom: Dez., Seite 934-938 (DE-627)NLEJ188984984 (DE-600)1462082-0 1432-203X nnns volume:15 year:1996 month:12 pages:934-938 extent:5 http://dx.doi.org/10.1007/BF00231591 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 15 1996 12 934-938 5 |
| allfieldsSound |
(DE-627)NLEJ203156897 DE-627 ger DE-627 rakwb eng Effect of media components on the gynogenic regeneration of onion (Allium cepa L.) cultivars and analysis of regenerants 1996 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary Two separate experiments were performed to analyze the effects of different media on gynogenic regeneration in four onion cultivars. In a two step flower/ovary culture procedure, 2,4-dichlorophenoxyacetic acid added to the induction medium was superior to phenylacetic acid in the highly regenerating cultivar, while the effect of thidiazuron in the regeneration medium was generally optimal in higher (2 mg/l) rather than in lower (0.2 mg/l) concentrations. Gellan-gum was compared to agar solidified media. A higher number of regenerants was achieved on the former, but an undesirable hyperhydricity of regenerants formed on gellan-gum solidified media greatly reduced the final survival of formed embryos. Analysis of the time interval needed for regeneration showed high variability (from 46 to 152 days after inoculation), which was particularly pronounced in genotypes with lower regeneration capacity. Simpler isozyme patterns of regenerants showed that all analysed regenerants of the cultivar with a high regeneration capacity were homozygous, while in the other three cultivars, a considerable percentage (11.1 to 36.4%) of heterozygous regenerants were also detected. Ploidy analysis of the regenerants with simpler isozyme patterns revealed that the majority of lines remain haploid. Identification of 2 homozygous triploid regenerants demonstrated that as in androgenesis, nuclear fusions can occur during gynogenic haploid regeneration. Springer Online Journal Archives 1860-2002 Jakše, M. oth Bohanec, B. oth Ihan, A. oth in Plant cell reports 1981 15(1996) vom: Dez., Seite 934-938 (DE-627)NLEJ188984984 (DE-600)1462082-0 1432-203X nnns volume:15 year:1996 month:12 pages:934-938 extent:5 http://dx.doi.org/10.1007/BF00231591 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 15 1996 12 934-938 5 |
| language |
English |
| source |
in Plant cell reports 15(1996) vom: Dez., Seite 934-938 volume:15 year:1996 month:12 pages:934-938 extent:5 |
| sourceStr |
in Plant cell reports 15(1996) vom: Dez., Seite 934-938 volume:15 year:1996 month:12 pages:934-938 extent:5 |
| format_phy_str_mv |
Article |
| institution |
findex.gbv.de |
| isfreeaccess_bool |
false |
| container_title |
Plant cell reports |
| authorswithroles_txt_mv |
Jakše, M. @@oth@@ Bohanec, B. @@oth@@ Ihan, A. @@oth@@ |
| publishDateDaySort_date |
1996-12-01T00:00:00Z |
| hierarchy_top_id |
NLEJ188984984 |
| id |
NLEJ203156897 |
| language_de |
englisch |
| fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ203156897</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210706120956.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070528s1996 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ203156897</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Effect of media components on the gynogenic regeneration of onion (Allium cepa L.) cultivars and analysis of regenerants</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1996</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">5</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Summary Two separate experiments were performed to analyze the effects of different media on gynogenic regeneration in four onion cultivars. In a two step flower/ovary culture procedure, 2,4-dichlorophenoxyacetic acid added to the induction medium was superior to phenylacetic acid in the highly regenerating cultivar, while the effect of thidiazuron in the regeneration medium was generally optimal in higher (2 mg/l) rather than in lower (0.2 mg/l) concentrations. Gellan-gum was compared to agar solidified media. A higher number of regenerants was achieved on the former, but an undesirable hyperhydricity of regenerants formed on gellan-gum solidified media greatly reduced the final survival of formed embryos. Analysis of the time interval needed for regeneration showed high variability (from 46 to 152 days after inoculation), which was particularly pronounced in genotypes with lower regeneration capacity. Simpler isozyme patterns of regenerants showed that all analysed regenerants of the cultivar with a high regeneration capacity were homozygous, while in the other three cultivars, a considerable percentage (11.1 to 36.4%) of heterozygous regenerants were also detected. Ploidy analysis of the regenerants with simpler isozyme patterns revealed that the majority of lines remain haploid. Identification of 2 homozygous triploid regenerants demonstrated that as in androgenesis, nuclear fusions can occur during gynogenic haploid regeneration.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Springer Online Journal Archives 1860-2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Jakše, M.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Bohanec, B.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Ihan, A.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">Plant cell reports</subfield><subfield code="d">1981</subfield><subfield code="g">15(1996) vom: Dez., Seite 934-938</subfield><subfield code="w">(DE-627)NLEJ188984984</subfield><subfield code="w">(DE-600)1462082-0</subfield><subfield code="x">1432-203X</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:15</subfield><subfield code="g">year:1996</subfield><subfield code="g">month:12</subfield><subfield code="g">pages:934-938</subfield><subfield code="g">extent:5</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1007/BF00231591</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SOJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">15</subfield><subfield code="j">1996</subfield><subfield code="c">12</subfield><subfield code="h">934-938</subfield><subfield code="g">5</subfield></datafield></record></collection>
|
| series2 |
Springer Online Journal Archives 1860-2002 |
| ppnlink_with_tag_str_mv |
@@773@@(DE-627)NLEJ188984984 |
| format |
electronic Article |
| delete_txt_mv |
keep |
| collection |
NL |
| remote_str |
true |
| illustrated |
Not Illustrated |
| issn |
1432-203X |
| topic_title |
Effect of media components on the gynogenic regeneration of onion (Allium cepa L.) cultivars and analysis of regenerants |
| format_facet |
Elektronische Aufsätze Aufsätze Elektronische Ressource |
| format_main_str_mv |
Text Zeitschrift/Artikel |
| carriertype_str_mv |
zu |
| author2_variant |
m j mj b b bb a i ai |
| hierarchy_parent_title |
Plant cell reports |
| hierarchy_parent_id |
NLEJ188984984 |
| hierarchy_top_title |
Plant cell reports |
| isfreeaccess_txt |
false |
| familylinks_str_mv |
(DE-627)NLEJ188984984 (DE-600)1462082-0 |
| title |
Effect of media components on the gynogenic regeneration of onion (Allium cepa L.) cultivars and analysis of regenerants |
| spellingShingle |
Effect of media components on the gynogenic regeneration of onion (Allium cepa L.) cultivars and analysis of regenerants |
| ctrlnum |
(DE-627)NLEJ203156897 |
| title_full |
Effect of media components on the gynogenic regeneration of onion (Allium cepa L.) cultivars and analysis of regenerants |
| journal |
Plant cell reports |
| journalStr |
Plant cell reports |
| lang_code |
eng |
| isOA_bool |
false |
| recordtype |
marc |
| publishDateSort |
1996 |
| contenttype_str_mv |
zzz |
| container_start_page |
934 |
| container_volume |
15 |
| physical |
5 |
| format_se |
Elektronische Aufsätze |
| title_sort |
effect of media components on the gynogenic regeneration of onion (allium cepa l.) cultivars and analysis of regenerants |
| title_auth |
Effect of media components on the gynogenic regeneration of onion (Allium cepa L.) cultivars and analysis of regenerants |
| abstract |
Summary Two separate experiments were performed to analyze the effects of different media on gynogenic regeneration in four onion cultivars. In a two step flower/ovary culture procedure, 2,4-dichlorophenoxyacetic acid added to the induction medium was superior to phenylacetic acid in the highly regenerating cultivar, while the effect of thidiazuron in the regeneration medium was generally optimal in higher (2 mg/l) rather than in lower (0.2 mg/l) concentrations. Gellan-gum was compared to agar solidified media. A higher number of regenerants was achieved on the former, but an undesirable hyperhydricity of regenerants formed on gellan-gum solidified media greatly reduced the final survival of formed embryos. Analysis of the time interval needed for regeneration showed high variability (from 46 to 152 days after inoculation), which was particularly pronounced in genotypes with lower regeneration capacity. Simpler isozyme patterns of regenerants showed that all analysed regenerants of the cultivar with a high regeneration capacity were homozygous, while in the other three cultivars, a considerable percentage (11.1 to 36.4%) of heterozygous regenerants were also detected. Ploidy analysis of the regenerants with simpler isozyme patterns revealed that the majority of lines remain haploid. Identification of 2 homozygous triploid regenerants demonstrated that as in androgenesis, nuclear fusions can occur during gynogenic haploid regeneration. |
| abstractGer |
Summary Two separate experiments were performed to analyze the effects of different media on gynogenic regeneration in four onion cultivars. In a two step flower/ovary culture procedure, 2,4-dichlorophenoxyacetic acid added to the induction medium was superior to phenylacetic acid in the highly regenerating cultivar, while the effect of thidiazuron in the regeneration medium was generally optimal in higher (2 mg/l) rather than in lower (0.2 mg/l) concentrations. Gellan-gum was compared to agar solidified media. A higher number of regenerants was achieved on the former, but an undesirable hyperhydricity of regenerants formed on gellan-gum solidified media greatly reduced the final survival of formed embryos. Analysis of the time interval needed for regeneration showed high variability (from 46 to 152 days after inoculation), which was particularly pronounced in genotypes with lower regeneration capacity. Simpler isozyme patterns of regenerants showed that all analysed regenerants of the cultivar with a high regeneration capacity were homozygous, while in the other three cultivars, a considerable percentage (11.1 to 36.4%) of heterozygous regenerants were also detected. Ploidy analysis of the regenerants with simpler isozyme patterns revealed that the majority of lines remain haploid. Identification of 2 homozygous triploid regenerants demonstrated that as in androgenesis, nuclear fusions can occur during gynogenic haploid regeneration. |
| abstract_unstemmed |
Summary Two separate experiments were performed to analyze the effects of different media on gynogenic regeneration in four onion cultivars. In a two step flower/ovary culture procedure, 2,4-dichlorophenoxyacetic acid added to the induction medium was superior to phenylacetic acid in the highly regenerating cultivar, while the effect of thidiazuron in the regeneration medium was generally optimal in higher (2 mg/l) rather than in lower (0.2 mg/l) concentrations. Gellan-gum was compared to agar solidified media. A higher number of regenerants was achieved on the former, but an undesirable hyperhydricity of regenerants formed on gellan-gum solidified media greatly reduced the final survival of formed embryos. Analysis of the time interval needed for regeneration showed high variability (from 46 to 152 days after inoculation), which was particularly pronounced in genotypes with lower regeneration capacity. Simpler isozyme patterns of regenerants showed that all analysed regenerants of the cultivar with a high regeneration capacity were homozygous, while in the other three cultivars, a considerable percentage (11.1 to 36.4%) of heterozygous regenerants were also detected. Ploidy analysis of the regenerants with simpler isozyme patterns revealed that the majority of lines remain haploid. Identification of 2 homozygous triploid regenerants demonstrated that as in androgenesis, nuclear fusions can occur during gynogenic haploid regeneration. |
| collection_details |
GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE |
| title_short |
Effect of media components on the gynogenic regeneration of onion (Allium cepa L.) cultivars and analysis of regenerants |
| url |
http://dx.doi.org/10.1007/BF00231591 |
| remote_bool |
true |
| author2 |
Jakše, M. Bohanec, B. Ihan, A. |
| author2Str |
Jakše, M. Bohanec, B. Ihan, A. |
| ppnlink |
NLEJ188984984 |
| mediatype_str_mv |
z |
| isOA_txt |
false |
| hochschulschrift_bool |
false |
| author2_role |
oth oth oth |
| up_date |
2024-07-06T09:57:07.910Z |
| _version_ |
1803823169310556160 |
| fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ203156897</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210706120956.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070528s1996 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ203156897</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Effect of media components on the gynogenic regeneration of onion (Allium cepa L.) cultivars and analysis of regenerants</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1996</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">5</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Summary Two separate experiments were performed to analyze the effects of different media on gynogenic regeneration in four onion cultivars. In a two step flower/ovary culture procedure, 2,4-dichlorophenoxyacetic acid added to the induction medium was superior to phenylacetic acid in the highly regenerating cultivar, while the effect of thidiazuron in the regeneration medium was generally optimal in higher (2 mg/l) rather than in lower (0.2 mg/l) concentrations. Gellan-gum was compared to agar solidified media. A higher number of regenerants was achieved on the former, but an undesirable hyperhydricity of regenerants formed on gellan-gum solidified media greatly reduced the final survival of formed embryos. Analysis of the time interval needed for regeneration showed high variability (from 46 to 152 days after inoculation), which was particularly pronounced in genotypes with lower regeneration capacity. Simpler isozyme patterns of regenerants showed that all analysed regenerants of the cultivar with a high regeneration capacity were homozygous, while in the other three cultivars, a considerable percentage (11.1 to 36.4%) of heterozygous regenerants were also detected. Ploidy analysis of the regenerants with simpler isozyme patterns revealed that the majority of lines remain haploid. Identification of 2 homozygous triploid regenerants demonstrated that as in androgenesis, nuclear fusions can occur during gynogenic haploid regeneration.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Springer Online Journal Archives 1860-2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Jakše, M.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Bohanec, B.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Ihan, A.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">Plant cell reports</subfield><subfield code="d">1981</subfield><subfield code="g">15(1996) vom: Dez., Seite 934-938</subfield><subfield code="w">(DE-627)NLEJ188984984</subfield><subfield code="w">(DE-600)1462082-0</subfield><subfield code="x">1432-203X</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:15</subfield><subfield code="g">year:1996</subfield><subfield code="g">month:12</subfield><subfield code="g">pages:934-938</subfield><subfield code="g">extent:5</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1007/BF00231591</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SOJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">15</subfield><subfield code="j">1996</subfield><subfield code="c">12</subfield><subfield code="h">934-938</subfield><subfield code="g">5</subfield></datafield></record></collection>
|
| score |
7.3987617 |