Modification of in vitro mouse embryogenesis by X-rays and fluorochromes
Abstract In view of planned studies using single particle irradiation at the Institute for Medical Radiobiology (IMR), confocal microscopy will become an important tool to visualise subtle changes in embryo morphology. Therefore, the influence of X-rays and that of three different fluorochromes on t...
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E-Artikel |
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Englisch |
| Erschienen: |
1994 |
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11 |
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Springer Online Journal Archives 1860-2002 |
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in: Radiation and environmental biophysics - 1963, 33(1994) vom: Apr., Seite 341-351 |
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volume:33 ; year:1994 ; month:04 ; pages:341-351 ; extent:11 |
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| 520 | |a Abstract In view of planned studies using single particle irradiation at the Institute for Medical Radiobiology (IMR), confocal microscopy will become an important tool to visualise subtle changes in embryo morphology. Therefore, the influence of X-rays and that of three different fluorochromes on the in vitro development of murine 2-cell stage embryos was investigated. Embryos of B6C3F1 mice were cultured at 32 h post-conception (pc) and treated with X-rays, acridine orange (AO), ethidium bromide (EB) or propidium iodide (PI), respectively, in various doses (0.0–3.0 Gy) or concentrations (AO: 0.05–2.00 µg/ml; EB and PI: 0.50–10.00 µg/ml). Additional experiments using combinations of AO and irradiation were performed. The embryos were cultivated for a total of 7 days and checked for their vital status by morphological endpoints such as the percentage of embryos that reached the blastocyst stage and the hatching rate. After treatment of the 2-cell embryos with AO, EB and PI, the dose-effect curves with the endpoint ‘hatching rate’ showed a 23-fold reduction of the ED50 for AO (0.23 µg/ ml) compared with EB (5.30 µg/ml). Despite the higher toxicity, AO had much better staining qualities in subtoxic concentrations than EB. PI showed no toxicity in these experiments and was used as an inverse control for embryo vitality. No synergistic modification of the radiogenic effects could be seen in combination experiments (AO+X-rays). | ||
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(DE-627)NLEJ20413577X DE-627 ger DE-627 rakwb eng Modification of in vitro mouse embryogenesis by X-rays and fluorochromes 1994 11 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract In view of planned studies using single particle irradiation at the Institute for Medical Radiobiology (IMR), confocal microscopy will become an important tool to visualise subtle changes in embryo morphology. Therefore, the influence of X-rays and that of three different fluorochromes on the in vitro development of murine 2-cell stage embryos was investigated. Embryos of B6C3F1 mice were cultured at 32 h post-conception (pc) and treated with X-rays, acridine orange (AO), ethidium bromide (EB) or propidium iodide (PI), respectively, in various doses (0.0–3.0 Gy) or concentrations (AO: 0.05–2.00 µg/ml; EB and PI: 0.50–10.00 µg/ml). Additional experiments using combinations of AO and irradiation were performed. The embryos were cultivated for a total of 7 days and checked for their vital status by morphological endpoints such as the percentage of embryos that reached the blastocyst stage and the hatching rate. After treatment of the 2-cell embryos with AO, EB and PI, the dose-effect curves with the endpoint ‘hatching rate’ showed a 23-fold reduction of the ED50 for AO (0.23 µg/ ml) compared with EB (5.30 µg/ml). Despite the higher toxicity, AO had much better staining qualities in subtoxic concentrations than EB. PI showed no toxicity in these experiments and was used as an inverse control for embryo vitality. No synergistic modification of the radiogenic effects could be seen in combination experiments (AO+X-rays). Springer Online Journal Archives 1860-2002 Kohler, M. oth Kündig, A. oth Reist, H. -W. oth Michel, C. oth in Radiation and environmental biophysics 1963 33(1994) vom: Apr., Seite 341-351 (DE-627)NLEJ188988556 (DE-600)1462083-2 1432-2099 nnns volume:33 year:1994 month:04 pages:341-351 extent:11 http://dx.doi.org/10.1007/BF01210455 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 33 1994 4 341-351 11 |
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(DE-627)NLEJ20413577X DE-627 ger DE-627 rakwb eng Modification of in vitro mouse embryogenesis by X-rays and fluorochromes 1994 11 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract In view of planned studies using single particle irradiation at the Institute for Medical Radiobiology (IMR), confocal microscopy will become an important tool to visualise subtle changes in embryo morphology. Therefore, the influence of X-rays and that of three different fluorochromes on the in vitro development of murine 2-cell stage embryos was investigated. Embryos of B6C3F1 mice were cultured at 32 h post-conception (pc) and treated with X-rays, acridine orange (AO), ethidium bromide (EB) or propidium iodide (PI), respectively, in various doses (0.0–3.0 Gy) or concentrations (AO: 0.05–2.00 µg/ml; EB and PI: 0.50–10.00 µg/ml). Additional experiments using combinations of AO and irradiation were performed. The embryos were cultivated for a total of 7 days and checked for their vital status by morphological endpoints such as the percentage of embryos that reached the blastocyst stage and the hatching rate. After treatment of the 2-cell embryos with AO, EB and PI, the dose-effect curves with the endpoint ‘hatching rate’ showed a 23-fold reduction of the ED50 for AO (0.23 µg/ ml) compared with EB (5.30 µg/ml). Despite the higher toxicity, AO had much better staining qualities in subtoxic concentrations than EB. PI showed no toxicity in these experiments and was used as an inverse control for embryo vitality. No synergistic modification of the radiogenic effects could be seen in combination experiments (AO+X-rays). Springer Online Journal Archives 1860-2002 Kohler, M. oth Kündig, A. oth Reist, H. -W. oth Michel, C. oth in Radiation and environmental biophysics 1963 33(1994) vom: Apr., Seite 341-351 (DE-627)NLEJ188988556 (DE-600)1462083-2 1432-2099 nnns volume:33 year:1994 month:04 pages:341-351 extent:11 http://dx.doi.org/10.1007/BF01210455 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 33 1994 4 341-351 11 |
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(DE-627)NLEJ20413577X DE-627 ger DE-627 rakwb eng Modification of in vitro mouse embryogenesis by X-rays and fluorochromes 1994 11 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract In view of planned studies using single particle irradiation at the Institute for Medical Radiobiology (IMR), confocal microscopy will become an important tool to visualise subtle changes in embryo morphology. Therefore, the influence of X-rays and that of three different fluorochromes on the in vitro development of murine 2-cell stage embryos was investigated. Embryos of B6C3F1 mice were cultured at 32 h post-conception (pc) and treated with X-rays, acridine orange (AO), ethidium bromide (EB) or propidium iodide (PI), respectively, in various doses (0.0–3.0 Gy) or concentrations (AO: 0.05–2.00 µg/ml; EB and PI: 0.50–10.00 µg/ml). Additional experiments using combinations of AO and irradiation were performed. The embryos were cultivated for a total of 7 days and checked for their vital status by morphological endpoints such as the percentage of embryos that reached the blastocyst stage and the hatching rate. After treatment of the 2-cell embryos with AO, EB and PI, the dose-effect curves with the endpoint ‘hatching rate’ showed a 23-fold reduction of the ED50 for AO (0.23 µg/ ml) compared with EB (5.30 µg/ml). Despite the higher toxicity, AO had much better staining qualities in subtoxic concentrations than EB. PI showed no toxicity in these experiments and was used as an inverse control for embryo vitality. No synergistic modification of the radiogenic effects could be seen in combination experiments (AO+X-rays). Springer Online Journal Archives 1860-2002 Kohler, M. oth Kündig, A. oth Reist, H. -W. oth Michel, C. oth in Radiation and environmental biophysics 1963 33(1994) vom: Apr., Seite 341-351 (DE-627)NLEJ188988556 (DE-600)1462083-2 1432-2099 nnns volume:33 year:1994 month:04 pages:341-351 extent:11 http://dx.doi.org/10.1007/BF01210455 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 33 1994 4 341-351 11 |
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(DE-627)NLEJ20413577X DE-627 ger DE-627 rakwb eng Modification of in vitro mouse embryogenesis by X-rays and fluorochromes 1994 11 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract In view of planned studies using single particle irradiation at the Institute for Medical Radiobiology (IMR), confocal microscopy will become an important tool to visualise subtle changes in embryo morphology. Therefore, the influence of X-rays and that of three different fluorochromes on the in vitro development of murine 2-cell stage embryos was investigated. Embryos of B6C3F1 mice were cultured at 32 h post-conception (pc) and treated with X-rays, acridine orange (AO), ethidium bromide (EB) or propidium iodide (PI), respectively, in various doses (0.0–3.0 Gy) or concentrations (AO: 0.05–2.00 µg/ml; EB and PI: 0.50–10.00 µg/ml). Additional experiments using combinations of AO and irradiation were performed. The embryos were cultivated for a total of 7 days and checked for their vital status by morphological endpoints such as the percentage of embryos that reached the blastocyst stage and the hatching rate. After treatment of the 2-cell embryos with AO, EB and PI, the dose-effect curves with the endpoint ‘hatching rate’ showed a 23-fold reduction of the ED50 for AO (0.23 µg/ ml) compared with EB (5.30 µg/ml). Despite the higher toxicity, AO had much better staining qualities in subtoxic concentrations than EB. PI showed no toxicity in these experiments and was used as an inverse control for embryo vitality. No synergistic modification of the radiogenic effects could be seen in combination experiments (AO+X-rays). Springer Online Journal Archives 1860-2002 Kohler, M. oth Kündig, A. oth Reist, H. -W. oth Michel, C. oth in Radiation and environmental biophysics 1963 33(1994) vom: Apr., Seite 341-351 (DE-627)NLEJ188988556 (DE-600)1462083-2 1432-2099 nnns volume:33 year:1994 month:04 pages:341-351 extent:11 http://dx.doi.org/10.1007/BF01210455 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 33 1994 4 341-351 11 |
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Modification of in vitro mouse embryogenesis by X-rays and fluorochromes |
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Abstract In view of planned studies using single particle irradiation at the Institute for Medical Radiobiology (IMR), confocal microscopy will become an important tool to visualise subtle changes in embryo morphology. Therefore, the influence of X-rays and that of three different fluorochromes on the in vitro development of murine 2-cell stage embryos was investigated. Embryos of B6C3F1 mice were cultured at 32 h post-conception (pc) and treated with X-rays, acridine orange (AO), ethidium bromide (EB) or propidium iodide (PI), respectively, in various doses (0.0–3.0 Gy) or concentrations (AO: 0.05–2.00 µg/ml; EB and PI: 0.50–10.00 µg/ml). Additional experiments using combinations of AO and irradiation were performed. The embryos were cultivated for a total of 7 days and checked for their vital status by morphological endpoints such as the percentage of embryos that reached the blastocyst stage and the hatching rate. After treatment of the 2-cell embryos with AO, EB and PI, the dose-effect curves with the endpoint ‘hatching rate’ showed a 23-fold reduction of the ED50 for AO (0.23 µg/ ml) compared with EB (5.30 µg/ml). Despite the higher toxicity, AO had much better staining qualities in subtoxic concentrations than EB. PI showed no toxicity in these experiments and was used as an inverse control for embryo vitality. No synergistic modification of the radiogenic effects could be seen in combination experiments (AO+X-rays). |
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Abstract In view of planned studies using single particle irradiation at the Institute for Medical Radiobiology (IMR), confocal microscopy will become an important tool to visualise subtle changes in embryo morphology. Therefore, the influence of X-rays and that of three different fluorochromes on the in vitro development of murine 2-cell stage embryos was investigated. Embryos of B6C3F1 mice were cultured at 32 h post-conception (pc) and treated with X-rays, acridine orange (AO), ethidium bromide (EB) or propidium iodide (PI), respectively, in various doses (0.0–3.0 Gy) or concentrations (AO: 0.05–2.00 µg/ml; EB and PI: 0.50–10.00 µg/ml). Additional experiments using combinations of AO and irradiation were performed. The embryos were cultivated for a total of 7 days and checked for their vital status by morphological endpoints such as the percentage of embryos that reached the blastocyst stage and the hatching rate. After treatment of the 2-cell embryos with AO, EB and PI, the dose-effect curves with the endpoint ‘hatching rate’ showed a 23-fold reduction of the ED50 for AO (0.23 µg/ ml) compared with EB (5.30 µg/ml). Despite the higher toxicity, AO had much better staining qualities in subtoxic concentrations than EB. PI showed no toxicity in these experiments and was used as an inverse control for embryo vitality. No synergistic modification of the radiogenic effects could be seen in combination experiments (AO+X-rays). |
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Abstract In view of planned studies using single particle irradiation at the Institute for Medical Radiobiology (IMR), confocal microscopy will become an important tool to visualise subtle changes in embryo morphology. Therefore, the influence of X-rays and that of three different fluorochromes on the in vitro development of murine 2-cell stage embryos was investigated. Embryos of B6C3F1 mice were cultured at 32 h post-conception (pc) and treated with X-rays, acridine orange (AO), ethidium bromide (EB) or propidium iodide (PI), respectively, in various doses (0.0–3.0 Gy) or concentrations (AO: 0.05–2.00 µg/ml; EB and PI: 0.50–10.00 µg/ml). Additional experiments using combinations of AO and irradiation were performed. The embryos were cultivated for a total of 7 days and checked for their vital status by morphological endpoints such as the percentage of embryos that reached the blastocyst stage and the hatching rate. After treatment of the 2-cell embryos with AO, EB and PI, the dose-effect curves with the endpoint ‘hatching rate’ showed a 23-fold reduction of the ED50 for AO (0.23 µg/ ml) compared with EB (5.30 µg/ml). Despite the higher toxicity, AO had much better staining qualities in subtoxic concentrations than EB. PI showed no toxicity in these experiments and was used as an inverse control for embryo vitality. No synergistic modification of the radiogenic effects could be seen in combination experiments (AO+X-rays). |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ20413577X</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210706143731.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070528s1994 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ20413577X</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Modification of in vitro mouse embryogenesis by X-rays and fluorochromes</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1994</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">11</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract In view of planned studies using single particle irradiation at the Institute for Medical Radiobiology (IMR), confocal microscopy will become an important tool to visualise subtle changes in embryo morphology. Therefore, the influence of X-rays and that of three different fluorochromes on the in vitro development of murine 2-cell stage embryos was investigated. Embryos of B6C3F1 mice were cultured at 32 h post-conception (pc) and treated with X-rays, acridine orange (AO), ethidium bromide (EB) or propidium iodide (PI), respectively, in various doses (0.0–3.0 Gy) or concentrations (AO: 0.05–2.00 µg/ml; EB and PI: 0.50–10.00 µg/ml). Additional experiments using combinations of AO and irradiation were performed. The embryos were cultivated for a total of 7 days and checked for their vital status by morphological endpoints such as the percentage of embryos that reached the blastocyst stage and the hatching rate. After treatment of the 2-cell embryos with AO, EB and PI, the dose-effect curves with the endpoint ‘hatching rate’ showed a 23-fold reduction of the ED50 for AO (0.23 µg/ ml) compared with EB (5.30 µg/ml). Despite the higher toxicity, AO had much better staining qualities in subtoxic concentrations than EB. PI showed no toxicity in these experiments and was used as an inverse control for embryo vitality. No synergistic modification of the radiogenic effects could be seen in combination experiments (AO+X-rays).</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Springer Online Journal Archives 1860-2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Kohler, M.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Kündig, A.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Reist, H. -W.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Michel, C.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">Radiation and environmental biophysics</subfield><subfield code="d">1963</subfield><subfield code="g">33(1994) vom: Apr., Seite 341-351</subfield><subfield code="w">(DE-627)NLEJ188988556</subfield><subfield code="w">(DE-600)1462083-2</subfield><subfield code="x">1432-2099</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:33</subfield><subfield code="g">year:1994</subfield><subfield code="g">month:04</subfield><subfield code="g">pages:341-351</subfield><subfield code="g">extent:11</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1007/BF01210455</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SOJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">33</subfield><subfield code="j">1994</subfield><subfield code="c">4</subfield><subfield code="h">341-351</subfield><subfield code="g">11</subfield></datafield></record></collection>
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