Tel2p, a regulator of yeast telomeric length in vivo, binds to single-stranded telomeric DNA in vitro
Abstract. The telomeres of the yeast Saccharomyces cerevisiae consist of a duplex region of TG1–3 repeats that acquire a single-stranded 3’ extension of the TG1–3 strand at the end of S-phase. The length of these repeats is kept within a defined range by regulators such as the TEL2-encoded protein (...
Ausführliche Beschreibung
Gespeichert in:
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1999 |
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| Umfang: |
13 |
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| Reproduktion: |
Springer Online Journal Archives 1860-2002 |
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| Übergeordnetes Werk: |
in: Chromosoma - 1939, 108(1999) vom: Mai, Seite 278-290 |
| Übergeordnetes Werk: |
volume:108 ; year:1999 ; month:05 ; pages:278-290 ; extent:13 |
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| 520 | |a Abstract. The telomeres of the yeast Saccharomyces cerevisiae consist of a duplex region of TG1–3 repeats that acquire a single-stranded 3’ extension of the TG1–3 strand at the end of S-phase. The length of these repeats is kept within a defined range by regulators such as the TEL2-encoded protein (Tel2p). Here we show that Tel2p can specifically bind to single-stranded TG1–3. Tel2p binding produced several shifted bands; however, only the slowest migrating band contained Tel2p. Methylation protection and interference experiments as well as gel shift experiments using inosine-containing probes indicated that the faster migrating bands resulted from Tel2p-mediated formation of DNA secondary structures held together by G-G interactions. Tel2p bound to single-stranded substrates that were at least 19 bases in length and contained 14 bases of TG1–3, and also to double-stranded/single-stranded hybrid substrates with a 3’ TG1–3 overhang. Tel2p binding to a hybrid substrate with a 24 base single-stranded TG1–3 extension also produced a band characteristic of G-G-mediated secondary structures. These data suggest that Tel2p could regulate telomeric length by binding to the 3’ single-stranded TG1–3 extension present at yeast telomeres. | ||
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(DE-627)NLEJ204149452 DE-627 ger DE-627 rakwb eng Tel2p, a regulator of yeast telomeric length in vivo, binds to single-stranded telomeric DNA in vitro 1999 13 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract. The telomeres of the yeast Saccharomyces cerevisiae consist of a duplex region of TG1–3 repeats that acquire a single-stranded 3’ extension of the TG1–3 strand at the end of S-phase. The length of these repeats is kept within a defined range by regulators such as the TEL2-encoded protein (Tel2p). Here we show that Tel2p can specifically bind to single-stranded TG1–3. Tel2p binding produced several shifted bands; however, only the slowest migrating band contained Tel2p. Methylation protection and interference experiments as well as gel shift experiments using inosine-containing probes indicated that the faster migrating bands resulted from Tel2p-mediated formation of DNA secondary structures held together by G-G interactions. Tel2p bound to single-stranded substrates that were at least 19 bases in length and contained 14 bases of TG1–3, and also to double-stranded/single-stranded hybrid substrates with a 3’ TG1–3 overhang. Tel2p binding to a hybrid substrate with a 24 base single-stranded TG1–3 extension also produced a band characteristic of G-G-mediated secondary structures. These data suggest that Tel2p could regulate telomeric length by binding to the 3’ single-stranded TG1–3 extension present at yeast telomeres. Springer Online Journal Archives 1860-2002 Kota, Rama S. oth Runge, Kurt W. oth in Chromosoma 1939 108(1999) vom: Mai, Seite 278-290 (DE-627)NLEJ188993002 (DE-600)1458507-8 1432-0886 nnns volume:108 year:1999 month:05 pages:278-290 extent:13 http://dx.doi.org/10.1007/s004120050379 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 108 1999 5 278-290 13 |
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(DE-627)NLEJ204149452 DE-627 ger DE-627 rakwb eng Tel2p, a regulator of yeast telomeric length in vivo, binds to single-stranded telomeric DNA in vitro 1999 13 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract. The telomeres of the yeast Saccharomyces cerevisiae consist of a duplex region of TG1–3 repeats that acquire a single-stranded 3’ extension of the TG1–3 strand at the end of S-phase. The length of these repeats is kept within a defined range by regulators such as the TEL2-encoded protein (Tel2p). Here we show that Tel2p can specifically bind to single-stranded TG1–3. Tel2p binding produced several shifted bands; however, only the slowest migrating band contained Tel2p. Methylation protection and interference experiments as well as gel shift experiments using inosine-containing probes indicated that the faster migrating bands resulted from Tel2p-mediated formation of DNA secondary structures held together by G-G interactions. Tel2p bound to single-stranded substrates that were at least 19 bases in length and contained 14 bases of TG1–3, and also to double-stranded/single-stranded hybrid substrates with a 3’ TG1–3 overhang. Tel2p binding to a hybrid substrate with a 24 base single-stranded TG1–3 extension also produced a band characteristic of G-G-mediated secondary structures. These data suggest that Tel2p could regulate telomeric length by binding to the 3’ single-stranded TG1–3 extension present at yeast telomeres. Springer Online Journal Archives 1860-2002 Kota, Rama S. oth Runge, Kurt W. oth in Chromosoma 1939 108(1999) vom: Mai, Seite 278-290 (DE-627)NLEJ188993002 (DE-600)1458507-8 1432-0886 nnns volume:108 year:1999 month:05 pages:278-290 extent:13 http://dx.doi.org/10.1007/s004120050379 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 108 1999 5 278-290 13 |
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(DE-627)NLEJ204149452 DE-627 ger DE-627 rakwb eng Tel2p, a regulator of yeast telomeric length in vivo, binds to single-stranded telomeric DNA in vitro 1999 13 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract. The telomeres of the yeast Saccharomyces cerevisiae consist of a duplex region of TG1–3 repeats that acquire a single-stranded 3’ extension of the TG1–3 strand at the end of S-phase. The length of these repeats is kept within a defined range by regulators such as the TEL2-encoded protein (Tel2p). Here we show that Tel2p can specifically bind to single-stranded TG1–3. Tel2p binding produced several shifted bands; however, only the slowest migrating band contained Tel2p. Methylation protection and interference experiments as well as gel shift experiments using inosine-containing probes indicated that the faster migrating bands resulted from Tel2p-mediated formation of DNA secondary structures held together by G-G interactions. Tel2p bound to single-stranded substrates that were at least 19 bases in length and contained 14 bases of TG1–3, and also to double-stranded/single-stranded hybrid substrates with a 3’ TG1–3 overhang. Tel2p binding to a hybrid substrate with a 24 base single-stranded TG1–3 extension also produced a band characteristic of G-G-mediated secondary structures. These data suggest that Tel2p could regulate telomeric length by binding to the 3’ single-stranded TG1–3 extension present at yeast telomeres. Springer Online Journal Archives 1860-2002 Kota, Rama S. oth Runge, Kurt W. oth in Chromosoma 1939 108(1999) vom: Mai, Seite 278-290 (DE-627)NLEJ188993002 (DE-600)1458507-8 1432-0886 nnns volume:108 year:1999 month:05 pages:278-290 extent:13 http://dx.doi.org/10.1007/s004120050379 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 108 1999 5 278-290 13 |
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(DE-627)NLEJ204149452 DE-627 ger DE-627 rakwb eng Tel2p, a regulator of yeast telomeric length in vivo, binds to single-stranded telomeric DNA in vitro 1999 13 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract. The telomeres of the yeast Saccharomyces cerevisiae consist of a duplex region of TG1–3 repeats that acquire a single-stranded 3’ extension of the TG1–3 strand at the end of S-phase. The length of these repeats is kept within a defined range by regulators such as the TEL2-encoded protein (Tel2p). Here we show that Tel2p can specifically bind to single-stranded TG1–3. Tel2p binding produced several shifted bands; however, only the slowest migrating band contained Tel2p. Methylation protection and interference experiments as well as gel shift experiments using inosine-containing probes indicated that the faster migrating bands resulted from Tel2p-mediated formation of DNA secondary structures held together by G-G interactions. Tel2p bound to single-stranded substrates that were at least 19 bases in length and contained 14 bases of TG1–3, and also to double-stranded/single-stranded hybrid substrates with a 3’ TG1–3 overhang. Tel2p binding to a hybrid substrate with a 24 base single-stranded TG1–3 extension also produced a band characteristic of G-G-mediated secondary structures. These data suggest that Tel2p could regulate telomeric length by binding to the 3’ single-stranded TG1–3 extension present at yeast telomeres. Springer Online Journal Archives 1860-2002 Kota, Rama S. oth Runge, Kurt W. oth in Chromosoma 1939 108(1999) vom: Mai, Seite 278-290 (DE-627)NLEJ188993002 (DE-600)1458507-8 1432-0886 nnns volume:108 year:1999 month:05 pages:278-290 extent:13 http://dx.doi.org/10.1007/s004120050379 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 108 1999 5 278-290 13 |
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(DE-627)NLEJ204149452 DE-627 ger DE-627 rakwb eng Tel2p, a regulator of yeast telomeric length in vivo, binds to single-stranded telomeric DNA in vitro 1999 13 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract. The telomeres of the yeast Saccharomyces cerevisiae consist of a duplex region of TG1–3 repeats that acquire a single-stranded 3’ extension of the TG1–3 strand at the end of S-phase. The length of these repeats is kept within a defined range by regulators such as the TEL2-encoded protein (Tel2p). Here we show that Tel2p can specifically bind to single-stranded TG1–3. Tel2p binding produced several shifted bands; however, only the slowest migrating band contained Tel2p. Methylation protection and interference experiments as well as gel shift experiments using inosine-containing probes indicated that the faster migrating bands resulted from Tel2p-mediated formation of DNA secondary structures held together by G-G interactions. Tel2p bound to single-stranded substrates that were at least 19 bases in length and contained 14 bases of TG1–3, and also to double-stranded/single-stranded hybrid substrates with a 3’ TG1–3 overhang. Tel2p binding to a hybrid substrate with a 24 base single-stranded TG1–3 extension also produced a band characteristic of G-G-mediated secondary structures. These data suggest that Tel2p could regulate telomeric length by binding to the 3’ single-stranded TG1–3 extension present at yeast telomeres. Springer Online Journal Archives 1860-2002 Kota, Rama S. oth Runge, Kurt W. oth in Chromosoma 1939 108(1999) vom: Mai, Seite 278-290 (DE-627)NLEJ188993002 (DE-600)1458507-8 1432-0886 nnns volume:108 year:1999 month:05 pages:278-290 extent:13 http://dx.doi.org/10.1007/s004120050379 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 108 1999 5 278-290 13 |
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Tel2p, a regulator of yeast telomeric length in vivo, binds to single-stranded telomeric DNA in vitro |
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Abstract. The telomeres of the yeast Saccharomyces cerevisiae consist of a duplex region of TG1–3 repeats that acquire a single-stranded 3’ extension of the TG1–3 strand at the end of S-phase. The length of these repeats is kept within a defined range by regulators such as the TEL2-encoded protein (Tel2p). Here we show that Tel2p can specifically bind to single-stranded TG1–3. Tel2p binding produced several shifted bands; however, only the slowest migrating band contained Tel2p. Methylation protection and interference experiments as well as gel shift experiments using inosine-containing probes indicated that the faster migrating bands resulted from Tel2p-mediated formation of DNA secondary structures held together by G-G interactions. Tel2p bound to single-stranded substrates that were at least 19 bases in length and contained 14 bases of TG1–3, and also to double-stranded/single-stranded hybrid substrates with a 3’ TG1–3 overhang. Tel2p binding to a hybrid substrate with a 24 base single-stranded TG1–3 extension also produced a band characteristic of G-G-mediated secondary structures. These data suggest that Tel2p could regulate telomeric length by binding to the 3’ single-stranded TG1–3 extension present at yeast telomeres. |
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Abstract. The telomeres of the yeast Saccharomyces cerevisiae consist of a duplex region of TG1–3 repeats that acquire a single-stranded 3’ extension of the TG1–3 strand at the end of S-phase. The length of these repeats is kept within a defined range by regulators such as the TEL2-encoded protein (Tel2p). Here we show that Tel2p can specifically bind to single-stranded TG1–3. Tel2p binding produced several shifted bands; however, only the slowest migrating band contained Tel2p. Methylation protection and interference experiments as well as gel shift experiments using inosine-containing probes indicated that the faster migrating bands resulted from Tel2p-mediated formation of DNA secondary structures held together by G-G interactions. Tel2p bound to single-stranded substrates that were at least 19 bases in length and contained 14 bases of TG1–3, and also to double-stranded/single-stranded hybrid substrates with a 3’ TG1–3 overhang. Tel2p binding to a hybrid substrate with a 24 base single-stranded TG1–3 extension also produced a band characteristic of G-G-mediated secondary structures. These data suggest that Tel2p could regulate telomeric length by binding to the 3’ single-stranded TG1–3 extension present at yeast telomeres. |
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Abstract. The telomeres of the yeast Saccharomyces cerevisiae consist of a duplex region of TG1–3 repeats that acquire a single-stranded 3’ extension of the TG1–3 strand at the end of S-phase. The length of these repeats is kept within a defined range by regulators such as the TEL2-encoded protein (Tel2p). Here we show that Tel2p can specifically bind to single-stranded TG1–3. Tel2p binding produced several shifted bands; however, only the slowest migrating band contained Tel2p. Methylation protection and interference experiments as well as gel shift experiments using inosine-containing probes indicated that the faster migrating bands resulted from Tel2p-mediated formation of DNA secondary structures held together by G-G interactions. Tel2p bound to single-stranded substrates that were at least 19 bases in length and contained 14 bases of TG1–3, and also to double-stranded/single-stranded hybrid substrates with a 3’ TG1–3 overhang. Tel2p binding to a hybrid substrate with a 24 base single-stranded TG1–3 extension also produced a band characteristic of G-G-mediated secondary structures. These data suggest that Tel2p could regulate telomeric length by binding to the 3’ single-stranded TG1–3 extension present at yeast telomeres. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ204149452</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210706143927.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070528s1999 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ204149452</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Tel2p, a regulator of yeast telomeric length in vivo, binds to single-stranded telomeric DNA in vitro</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1999</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">13</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract. The telomeres of the yeast Saccharomyces cerevisiae consist of a duplex region of TG1–3 repeats that acquire a single-stranded 3’ extension of the TG1–3 strand at the end of S-phase. The length of these repeats is kept within a defined range by regulators such as the TEL2-encoded protein (Tel2p). Here we show that Tel2p can specifically bind to single-stranded TG1–3. Tel2p binding produced several shifted bands; however, only the slowest migrating band contained Tel2p. Methylation protection and interference experiments as well as gel shift experiments using inosine-containing probes indicated that the faster migrating bands resulted from Tel2p-mediated formation of DNA secondary structures held together by G-G interactions. Tel2p bound to single-stranded substrates that were at least 19 bases in length and contained 14 bases of TG1–3, and also to double-stranded/single-stranded hybrid substrates with a 3’ TG1–3 overhang. Tel2p binding to a hybrid substrate with a 24 base single-stranded TG1–3 extension also produced a band characteristic of G-G-mediated secondary structures. These data suggest that Tel2p could regulate telomeric length by binding to the 3’ single-stranded TG1–3 extension present at yeast telomeres.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Springer Online Journal Archives 1860-2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Kota, Rama S.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Runge, Kurt W.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">Chromosoma</subfield><subfield code="d">1939</subfield><subfield code="g">108(1999) vom: Mai, Seite 278-290</subfield><subfield code="w">(DE-627)NLEJ188993002</subfield><subfield code="w">(DE-600)1458507-8</subfield><subfield code="x">1432-0886</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:108</subfield><subfield code="g">year:1999</subfield><subfield code="g">month:05</subfield><subfield code="g">pages:278-290</subfield><subfield code="g">extent:13</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1007/s004120050379</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SOJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">108</subfield><subfield code="j">1999</subfield><subfield code="c">5</subfield><subfield code="h">278-290</subfield><subfield code="g">13</subfield></datafield></record></collection>
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7.399946 |