Immunocytochemical localization of γ-glutamyl-transferase on isolated renal cortical tubular fragments
Summary The localization of γ-Glutamyltransferase (γ-GT, E.C.2.3.2.2.) was studied on isolated tubular fragments from rat kidney cortex immunocytochemically. Monospecific antibodies raised in the goat against rat kidney γ-GT were used. Antigoat immunoglobulin from the rabbit conjugated with ferritin...
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1984 |
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| Umfang: |
5 |
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| Reproduktion: |
Springer Online Journal Archives 1860-2002 |
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| Übergeordnetes Werk: |
in: Histochemistry and cell biology - 1958, 80(1984) vom: März, Seite 289-293 |
| Übergeordnetes Werk: |
volume:80 ; year:1984 ; month:03 ; pages:289-293 ; extent:5 |
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NLEJ204402328 |
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| 245 | 1 | 0 | |a Immunocytochemical localization of γ-glutamyl-transferase on isolated renal cortical tubular fragments |
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| 520 | |a Summary The localization of γ-Glutamyltransferase (γ-GT, E.C.2.3.2.2.) was studied on isolated tubular fragments from rat kidney cortex immunocytochemically. Monospecific antibodies raised in the goat against rat kidney γ-GT were used. Antigoat immunoglobulin from the rabbit conjugated with ferritin was used for visualisation of the antibody binding sites. The enzyme was found to be localized at the brush border membrane of proximal tubules, the luminal membrane of distal tubules and collecting duct segments. The enzyme could further be localized on the antiluminal or basolateral cell membranes of proximal and distal tubular fragments, whereas no such localization was verified for collecting duct segments. The role of this basolateral γ-GT localization in context with the kidney's ability to extract over 83% of the renal arterial glutathione (GSH) input during a single passage is discussed. | ||
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| 700 | 1 | |a Gstraunthaler, G. |4 oth | |
| 700 | 1 | |a Kotanko, P. |4 oth | |
| 700 | 1 | |a Wolf, H. |4 oth | |
| 700 | 1 | |a Curthoys, N. P. |4 oth | |
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(DE-627)NLEJ204402328 DE-627 ger DE-627 rakwb eng Immunocytochemical localization of γ-glutamyl-transferase on isolated renal cortical tubular fragments 1984 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary The localization of γ-Glutamyltransferase (γ-GT, E.C.2.3.2.2.) was studied on isolated tubular fragments from rat kidney cortex immunocytochemically. Monospecific antibodies raised in the goat against rat kidney γ-GT were used. Antigoat immunoglobulin from the rabbit conjugated with ferritin was used for visualisation of the antibody binding sites. The enzyme was found to be localized at the brush border membrane of proximal tubules, the luminal membrane of distal tubules and collecting duct segments. The enzyme could further be localized on the antiluminal or basolateral cell membranes of proximal and distal tubular fragments, whereas no such localization was verified for collecting duct segments. The role of this basolateral γ-GT localization in context with the kidney's ability to extract over 83% of the renal arterial glutathione (GSH) input during a single passage is discussed. Springer Online Journal Archives 1860-2002 Pfaller, W. oth Gstraunthaler, G. oth Kotanko, P. oth Wolf, H. oth Curthoys, N. P. oth in Histochemistry and cell biology 1958 80(1984) vom: März, Seite 289-293 (DE-627)NLEJ188988467 (DE-600)1398345-3 1432-119X nnns volume:80 year:1984 month:03 pages:289-293 extent:5 http://dx.doi.org/10.1007/BF00495779 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 80 1984 3 289-293 5 |
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(DE-627)NLEJ204402328 DE-627 ger DE-627 rakwb eng Immunocytochemical localization of γ-glutamyl-transferase on isolated renal cortical tubular fragments 1984 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary The localization of γ-Glutamyltransferase (γ-GT, E.C.2.3.2.2.) was studied on isolated tubular fragments from rat kidney cortex immunocytochemically. Monospecific antibodies raised in the goat against rat kidney γ-GT were used. Antigoat immunoglobulin from the rabbit conjugated with ferritin was used for visualisation of the antibody binding sites. The enzyme was found to be localized at the brush border membrane of proximal tubules, the luminal membrane of distal tubules and collecting duct segments. The enzyme could further be localized on the antiluminal or basolateral cell membranes of proximal and distal tubular fragments, whereas no such localization was verified for collecting duct segments. The role of this basolateral γ-GT localization in context with the kidney's ability to extract over 83% of the renal arterial glutathione (GSH) input during a single passage is discussed. Springer Online Journal Archives 1860-2002 Pfaller, W. oth Gstraunthaler, G. oth Kotanko, P. oth Wolf, H. oth Curthoys, N. P. oth in Histochemistry and cell biology 1958 80(1984) vom: März, Seite 289-293 (DE-627)NLEJ188988467 (DE-600)1398345-3 1432-119X nnns volume:80 year:1984 month:03 pages:289-293 extent:5 http://dx.doi.org/10.1007/BF00495779 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 80 1984 3 289-293 5 |
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(DE-627)NLEJ204402328 DE-627 ger DE-627 rakwb eng Immunocytochemical localization of γ-glutamyl-transferase on isolated renal cortical tubular fragments 1984 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary The localization of γ-Glutamyltransferase (γ-GT, E.C.2.3.2.2.) was studied on isolated tubular fragments from rat kidney cortex immunocytochemically. Monospecific antibodies raised in the goat against rat kidney γ-GT were used. Antigoat immunoglobulin from the rabbit conjugated with ferritin was used for visualisation of the antibody binding sites. The enzyme was found to be localized at the brush border membrane of proximal tubules, the luminal membrane of distal tubules and collecting duct segments. The enzyme could further be localized on the antiluminal or basolateral cell membranes of proximal and distal tubular fragments, whereas no such localization was verified for collecting duct segments. The role of this basolateral γ-GT localization in context with the kidney's ability to extract over 83% of the renal arterial glutathione (GSH) input during a single passage is discussed. Springer Online Journal Archives 1860-2002 Pfaller, W. oth Gstraunthaler, G. oth Kotanko, P. oth Wolf, H. oth Curthoys, N. P. oth in Histochemistry and cell biology 1958 80(1984) vom: März, Seite 289-293 (DE-627)NLEJ188988467 (DE-600)1398345-3 1432-119X nnns volume:80 year:1984 month:03 pages:289-293 extent:5 http://dx.doi.org/10.1007/BF00495779 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 80 1984 3 289-293 5 |
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(DE-627)NLEJ204402328 DE-627 ger DE-627 rakwb eng Immunocytochemical localization of γ-glutamyl-transferase on isolated renal cortical tubular fragments 1984 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary The localization of γ-Glutamyltransferase (γ-GT, E.C.2.3.2.2.) was studied on isolated tubular fragments from rat kidney cortex immunocytochemically. Monospecific antibodies raised in the goat against rat kidney γ-GT were used. Antigoat immunoglobulin from the rabbit conjugated with ferritin was used for visualisation of the antibody binding sites. The enzyme was found to be localized at the brush border membrane of proximal tubules, the luminal membrane of distal tubules and collecting duct segments. The enzyme could further be localized on the antiluminal or basolateral cell membranes of proximal and distal tubular fragments, whereas no such localization was verified for collecting duct segments. The role of this basolateral γ-GT localization in context with the kidney's ability to extract over 83% of the renal arterial glutathione (GSH) input during a single passage is discussed. Springer Online Journal Archives 1860-2002 Pfaller, W. oth Gstraunthaler, G. oth Kotanko, P. oth Wolf, H. oth Curthoys, N. P. oth in Histochemistry and cell biology 1958 80(1984) vom: März, Seite 289-293 (DE-627)NLEJ188988467 (DE-600)1398345-3 1432-119X nnns volume:80 year:1984 month:03 pages:289-293 extent:5 http://dx.doi.org/10.1007/BF00495779 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 80 1984 3 289-293 5 |
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(DE-627)NLEJ204402328 DE-627 ger DE-627 rakwb eng Immunocytochemical localization of γ-glutamyl-transferase on isolated renal cortical tubular fragments 1984 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary The localization of γ-Glutamyltransferase (γ-GT, E.C.2.3.2.2.) was studied on isolated tubular fragments from rat kidney cortex immunocytochemically. Monospecific antibodies raised in the goat against rat kidney γ-GT were used. Antigoat immunoglobulin from the rabbit conjugated with ferritin was used for visualisation of the antibody binding sites. The enzyme was found to be localized at the brush border membrane of proximal tubules, the luminal membrane of distal tubules and collecting duct segments. The enzyme could further be localized on the antiluminal or basolateral cell membranes of proximal and distal tubular fragments, whereas no such localization was verified for collecting duct segments. The role of this basolateral γ-GT localization in context with the kidney's ability to extract over 83% of the renal arterial glutathione (GSH) input during a single passage is discussed. Springer Online Journal Archives 1860-2002 Pfaller, W. oth Gstraunthaler, G. oth Kotanko, P. oth Wolf, H. oth Curthoys, N. P. oth in Histochemistry and cell biology 1958 80(1984) vom: März, Seite 289-293 (DE-627)NLEJ188988467 (DE-600)1398345-3 1432-119X nnns volume:80 year:1984 month:03 pages:289-293 extent:5 http://dx.doi.org/10.1007/BF00495779 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 80 1984 3 289-293 5 |
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in Histochemistry and cell biology 80(1984) vom: März, Seite 289-293 volume:80 year:1984 month:03 pages:289-293 extent:5 |
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immunocytochemical localization of γ-glutamyl-transferase on isolated renal cortical tubular fragments |
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Immunocytochemical localization of γ-glutamyl-transferase on isolated renal cortical tubular fragments |
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Summary The localization of γ-Glutamyltransferase (γ-GT, E.C.2.3.2.2.) was studied on isolated tubular fragments from rat kidney cortex immunocytochemically. Monospecific antibodies raised in the goat against rat kidney γ-GT were used. Antigoat immunoglobulin from the rabbit conjugated with ferritin was used for visualisation of the antibody binding sites. The enzyme was found to be localized at the brush border membrane of proximal tubules, the luminal membrane of distal tubules and collecting duct segments. The enzyme could further be localized on the antiluminal or basolateral cell membranes of proximal and distal tubular fragments, whereas no such localization was verified for collecting duct segments. The role of this basolateral γ-GT localization in context with the kidney's ability to extract over 83% of the renal arterial glutathione (GSH) input during a single passage is discussed. |
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Summary The localization of γ-Glutamyltransferase (γ-GT, E.C.2.3.2.2.) was studied on isolated tubular fragments from rat kidney cortex immunocytochemically. Monospecific antibodies raised in the goat against rat kidney γ-GT were used. Antigoat immunoglobulin from the rabbit conjugated with ferritin was used for visualisation of the antibody binding sites. The enzyme was found to be localized at the brush border membrane of proximal tubules, the luminal membrane of distal tubules and collecting duct segments. The enzyme could further be localized on the antiluminal or basolateral cell membranes of proximal and distal tubular fragments, whereas no such localization was verified for collecting duct segments. The role of this basolateral γ-GT localization in context with the kidney's ability to extract over 83% of the renal arterial glutathione (GSH) input during a single passage is discussed. |
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Summary The localization of γ-Glutamyltransferase (γ-GT, E.C.2.3.2.2.) was studied on isolated tubular fragments from rat kidney cortex immunocytochemically. Monospecific antibodies raised in the goat against rat kidney γ-GT were used. Antigoat immunoglobulin from the rabbit conjugated with ferritin was used for visualisation of the antibody binding sites. The enzyme was found to be localized at the brush border membrane of proximal tubules, the luminal membrane of distal tubules and collecting duct segments. The enzyme could further be localized on the antiluminal or basolateral cell membranes of proximal and distal tubular fragments, whereas no such localization was verified for collecting duct segments. The role of this basolateral γ-GT localization in context with the kidney's ability to extract over 83% of the renal arterial glutathione (GSH) input during a single passage is discussed. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ204402328</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210706151758.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070528s1984 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ204402328</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Immunocytochemical localization of γ-glutamyl-transferase on isolated renal cortical tubular fragments</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1984</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">5</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Summary The localization of γ-Glutamyltransferase (γ-GT, E.C.2.3.2.2.) was studied on isolated tubular fragments from rat kidney cortex immunocytochemically. Monospecific antibodies raised in the goat against rat kidney γ-GT were used. Antigoat immunoglobulin from the rabbit conjugated with ferritin was used for visualisation of the antibody binding sites. The enzyme was found to be localized at the brush border membrane of proximal tubules, the luminal membrane of distal tubules and collecting duct segments. The enzyme could further be localized on the antiluminal or basolateral cell membranes of proximal and distal tubular fragments, whereas no such localization was verified for collecting duct segments. The role of this basolateral γ-GT localization in context with the kidney's ability to extract over 83% of the renal arterial glutathione (GSH) input during a single passage is discussed.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Springer Online Journal Archives 1860-2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Pfaller, W.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Gstraunthaler, G.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Kotanko, P.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Wolf, H.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Curthoys, N. P.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">Histochemistry and cell biology</subfield><subfield code="d">1958</subfield><subfield code="g">80(1984) vom: März, Seite 289-293</subfield><subfield code="w">(DE-627)NLEJ188988467</subfield><subfield code="w">(DE-600)1398345-3</subfield><subfield code="x">1432-119X</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:80</subfield><subfield code="g">year:1984</subfield><subfield code="g">month:03</subfield><subfield code="g">pages:289-293</subfield><subfield code="g">extent:5</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1007/BF00495779</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SOJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">80</subfield><subfield code="j">1984</subfield><subfield code="c">3</subfield><subfield code="h">289-293</subfield><subfield code="g">5</subfield></datafield></record></collection>
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