Construction and rapid screening of a representative yeast artificial chromosome library from the Plasmodium falciparum strain Dd2
Abstract Large genomic DNA fragments from the Plasmodium falciparum clone Dd2 have been cloned as artificial chromosomes in yeast (YAC). The resulting library has a 10-fold redundancy for single-copy genes and consists of 1440 individual clones, including 240 telomeric clones, with an average inser...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1996 |
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| Umfang: |
3 |
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| Reproduktion: |
Springer Online Journal Archives 1860-2002 |
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| Übergeordnetes Werk: |
volume:83 ; year:1996 ; month:01 ; pages:87-89 ; extent:3 |
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| 520 | |a Abstract Large genomic DNA fragments from the Plasmodium falciparum clone Dd2 have been cloned as artificial chromosomes in yeast (YAC). The resulting library has a 10-fold redundancy for single-copy genes and consists of 1440 individual clones, including 240 telomeric clones, with an average insert size of 150 kb. A novel hybridization method was developed for the rapid and cost-effective screening of protozoan YAC libraries. The Dd2 YAC clones will facilitate a positional approach to the parasite’s genes and aid in the dissection of genetic loci associated with the virulence and pathogenicity of P. falciparum. | ||
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(DE-627)NLEJ205421288 DE-627 ger DE-627 rakwb eng Construction and rapid screening of a representative yeast artificial chromosome library from the Plasmodium falciparum strain Dd2 1996 3 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract Large genomic DNA fragments from the Plasmodium falciparum clone Dd2 have been cloned as artificial chromosomes in yeast (YAC). The resulting library has a 10-fold redundancy for single-copy genes and consists of 1440 individual clones, including 240 telomeric clones, with an average insert size of 150 kb. A novel hybridization method was developed for the rapid and cost-effective screening of protozoan YAC libraries. The Dd2 YAC clones will facilitate a positional approach to the parasite’s genes and aid in the dissection of genetic loci associated with the virulence and pathogenicity of P. falciparum. Springer Online Journal Archives 1860-2002 Camargo, Anamaria A. oth Fischer, Katja oth Lanzer, M. oth volume:83 year:1996 month:01 pages:87-89 extent:3 http://dx.doi.org/10.1007/s004360050214 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 83 1996 1 87-89 3 |
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(DE-627)NLEJ205421288 DE-627 ger DE-627 rakwb eng Construction and rapid screening of a representative yeast artificial chromosome library from the Plasmodium falciparum strain Dd2 1996 3 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract Large genomic DNA fragments from the Plasmodium falciparum clone Dd2 have been cloned as artificial chromosomes in yeast (YAC). The resulting library has a 10-fold redundancy for single-copy genes and consists of 1440 individual clones, including 240 telomeric clones, with an average insert size of 150 kb. A novel hybridization method was developed for the rapid and cost-effective screening of protozoan YAC libraries. The Dd2 YAC clones will facilitate a positional approach to the parasite’s genes and aid in the dissection of genetic loci associated with the virulence and pathogenicity of P. falciparum. Springer Online Journal Archives 1860-2002 Camargo, Anamaria A. oth Fischer, Katja oth Lanzer, M. oth volume:83 year:1996 month:01 pages:87-89 extent:3 http://dx.doi.org/10.1007/s004360050214 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 83 1996 1 87-89 3 |
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(DE-627)NLEJ205421288 DE-627 ger DE-627 rakwb eng Construction and rapid screening of a representative yeast artificial chromosome library from the Plasmodium falciparum strain Dd2 1996 3 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract Large genomic DNA fragments from the Plasmodium falciparum clone Dd2 have been cloned as artificial chromosomes in yeast (YAC). The resulting library has a 10-fold redundancy for single-copy genes and consists of 1440 individual clones, including 240 telomeric clones, with an average insert size of 150 kb. A novel hybridization method was developed for the rapid and cost-effective screening of protozoan YAC libraries. The Dd2 YAC clones will facilitate a positional approach to the parasite’s genes and aid in the dissection of genetic loci associated with the virulence and pathogenicity of P. falciparum. Springer Online Journal Archives 1860-2002 Camargo, Anamaria A. oth Fischer, Katja oth Lanzer, M. oth volume:83 year:1996 month:01 pages:87-89 extent:3 http://dx.doi.org/10.1007/s004360050214 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 83 1996 1 87-89 3 |
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(DE-627)NLEJ205421288 DE-627 ger DE-627 rakwb eng Construction and rapid screening of a representative yeast artificial chromosome library from the Plasmodium falciparum strain Dd2 1996 3 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract Large genomic DNA fragments from the Plasmodium falciparum clone Dd2 have been cloned as artificial chromosomes in yeast (YAC). The resulting library has a 10-fold redundancy for single-copy genes and consists of 1440 individual clones, including 240 telomeric clones, with an average insert size of 150 kb. A novel hybridization method was developed for the rapid and cost-effective screening of protozoan YAC libraries. The Dd2 YAC clones will facilitate a positional approach to the parasite’s genes and aid in the dissection of genetic loci associated with the virulence and pathogenicity of P. falciparum. Springer Online Journal Archives 1860-2002 Camargo, Anamaria A. oth Fischer, Katja oth Lanzer, M. oth volume:83 year:1996 month:01 pages:87-89 extent:3 http://dx.doi.org/10.1007/s004360050214 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 83 1996 1 87-89 3 |
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(DE-627)NLEJ205421288 DE-627 ger DE-627 rakwb eng Construction and rapid screening of a representative yeast artificial chromosome library from the Plasmodium falciparum strain Dd2 1996 3 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract Large genomic DNA fragments from the Plasmodium falciparum clone Dd2 have been cloned as artificial chromosomes in yeast (YAC). The resulting library has a 10-fold redundancy for single-copy genes and consists of 1440 individual clones, including 240 telomeric clones, with an average insert size of 150 kb. A novel hybridization method was developed for the rapid and cost-effective screening of protozoan YAC libraries. The Dd2 YAC clones will facilitate a positional approach to the parasite’s genes and aid in the dissection of genetic loci associated with the virulence and pathogenicity of P. falciparum. Springer Online Journal Archives 1860-2002 Camargo, Anamaria A. oth Fischer, Katja oth Lanzer, M. oth volume:83 year:1996 month:01 pages:87-89 extent:3 http://dx.doi.org/10.1007/s004360050214 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 83 1996 1 87-89 3 |
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Construction and rapid screening of a representative yeast artificial chromosome library from the Plasmodium falciparum strain Dd2 |
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construction and rapid screening of a representative yeast artificial chromosome library from the plasmodium falciparum strain dd2 |
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Construction and rapid screening of a representative yeast artificial chromosome library from the Plasmodium falciparum strain Dd2 |
| abstract |
Abstract Large genomic DNA fragments from the Plasmodium falciparum clone Dd2 have been cloned as artificial chromosomes in yeast (YAC). The resulting library has a 10-fold redundancy for single-copy genes and consists of 1440 individual clones, including 240 telomeric clones, with an average insert size of 150 kb. A novel hybridization method was developed for the rapid and cost-effective screening of protozoan YAC libraries. The Dd2 YAC clones will facilitate a positional approach to the parasite’s genes and aid in the dissection of genetic loci associated with the virulence and pathogenicity of P. falciparum. |
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Abstract Large genomic DNA fragments from the Plasmodium falciparum clone Dd2 have been cloned as artificial chromosomes in yeast (YAC). The resulting library has a 10-fold redundancy for single-copy genes and consists of 1440 individual clones, including 240 telomeric clones, with an average insert size of 150 kb. A novel hybridization method was developed for the rapid and cost-effective screening of protozoan YAC libraries. The Dd2 YAC clones will facilitate a positional approach to the parasite’s genes and aid in the dissection of genetic loci associated with the virulence and pathogenicity of P. falciparum. |
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Abstract Large genomic DNA fragments from the Plasmodium falciparum clone Dd2 have been cloned as artificial chromosomes in yeast (YAC). The resulting library has a 10-fold redundancy for single-copy genes and consists of 1440 individual clones, including 240 telomeric clones, with an average insert size of 150 kb. A novel hybridization method was developed for the rapid and cost-effective screening of protozoan YAC libraries. The Dd2 YAC clones will facilitate a positional approach to the parasite’s genes and aid in the dissection of genetic loci associated with the virulence and pathogenicity of P. falciparum. |
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Construction and rapid screening of a representative yeast artificial chromosome library from the Plasmodium falciparum strain Dd2 |
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