Localization of genes responsible for replication and immunity to colicin A on plasmid ColA-CA31
Summary The region containing the origin and regulatory sites for replication as well as the immunity gene (iaa) have been localized on the plasmid ColA-CA31. The region involved in replication functions of ColA can be hybridized with that of ColE1. It is located between 0 and 1 Kb on the plasmid ma...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1983 |
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| Umfang: |
6 |
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| Reproduktion: |
Springer Online Journal Archives 1860-2002 |
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| Übergeordnetes Werk: |
in: Molecular genetics and genomics - 1908, 192(1983) vom: März, Seite 500-505 |
| Übergeordnetes Werk: |
volume:192 ; year:1983 ; month:03 ; pages:500-505 ; extent:6 |
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NLEJ205477070 |
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| 245 | 1 | 0 | |a Localization of genes responsible for replication and immunity to colicin A on plasmid ColA-CA31 |
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| 520 | |a Summary The region containing the origin and regulatory sites for replication as well as the immunity gene (iaa) have been localized on the plasmid ColA-CA31. The region involved in replication functions of ColA can be hybridized with that of ColE1. It is located between 0 and 1 Kb on the plasmid map previously published (Morlon et al. 1982a). A 0.50 Kb HincII fragment of ColA can be weakly hybridized to the ColE1 immunity region. This fragment contains iaa since directed in vitro mutagenesis at an internal restriction site can abolish the immunity to colicin A; however, it does not contain the entire iaa. Knowing the localization of regions involved in autonomous DNA replication and immunity, a mini-ColA plasmid was constructed that contains these two regions. The mini-ColA of 2.8 Kb can be amplified in the presence of chloramphenicol and confers the immunity to transformants. It thus constitutes a useful cloning vector. Expression of ColA and of the various constructed plasmids in the maxicell system suggests that the immunity protein has a molecular weight of about 18–20 Kd. | ||
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| 700 | 1 | |a Lazdunski, Claude |4 oth | |
| 700 | 1 | |a Cavard, Danielle |4 oth | |
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(DE-627)NLEJ205477070 DE-627 ger DE-627 rakwb eng Localization of genes responsible for replication and immunity to colicin A on plasmid ColA-CA31 1983 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary The region containing the origin and regulatory sites for replication as well as the immunity gene (iaa) have been localized on the plasmid ColA-CA31. The region involved in replication functions of ColA can be hybridized with that of ColE1. It is located between 0 and 1 Kb on the plasmid map previously published (Morlon et al. 1982a). A 0.50 Kb HincII fragment of ColA can be weakly hybridized to the ColE1 immunity region. This fragment contains iaa since directed in vitro mutagenesis at an internal restriction site can abolish the immunity to colicin A; however, it does not contain the entire iaa. Knowing the localization of regions involved in autonomous DNA replication and immunity, a mini-ColA plasmid was constructed that contains these two regions. The mini-ColA of 2.8 Kb can be amplified in the presence of chloramphenicol and confers the immunity to transformants. It thus constitutes a useful cloning vector. Expression of ColA and of the various constructed plasmids in the maxicell system suggests that the immunity protein has a molecular weight of about 18–20 Kd. Springer Online Journal Archives 1860-2002 Crozel, Veronica oth Lazdunski, Claude oth Cavard, Danielle oth in Molecular genetics and genomics 1908 192(1983) vom: März, Seite 500-505 (DE-627)NLEJ188991441 (DE-600)1462070-4 1617-4623 nnns volume:192 year:1983 month:03 pages:500-505 extent:6 http://dx.doi.org/10.1007/BF00392196 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 192 1983 3 500-505 6 |
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(DE-627)NLEJ205477070 DE-627 ger DE-627 rakwb eng Localization of genes responsible for replication and immunity to colicin A on plasmid ColA-CA31 1983 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary The region containing the origin and regulatory sites for replication as well as the immunity gene (iaa) have been localized on the plasmid ColA-CA31. The region involved in replication functions of ColA can be hybridized with that of ColE1. It is located between 0 and 1 Kb on the plasmid map previously published (Morlon et al. 1982a). A 0.50 Kb HincII fragment of ColA can be weakly hybridized to the ColE1 immunity region. This fragment contains iaa since directed in vitro mutagenesis at an internal restriction site can abolish the immunity to colicin A; however, it does not contain the entire iaa. Knowing the localization of regions involved in autonomous DNA replication and immunity, a mini-ColA plasmid was constructed that contains these two regions. The mini-ColA of 2.8 Kb can be amplified in the presence of chloramphenicol and confers the immunity to transformants. It thus constitutes a useful cloning vector. Expression of ColA and of the various constructed plasmids in the maxicell system suggests that the immunity protein has a molecular weight of about 18–20 Kd. Springer Online Journal Archives 1860-2002 Crozel, Veronica oth Lazdunski, Claude oth Cavard, Danielle oth in Molecular genetics and genomics 1908 192(1983) vom: März, Seite 500-505 (DE-627)NLEJ188991441 (DE-600)1462070-4 1617-4623 nnns volume:192 year:1983 month:03 pages:500-505 extent:6 http://dx.doi.org/10.1007/BF00392196 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 192 1983 3 500-505 6 |
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(DE-627)NLEJ205477070 DE-627 ger DE-627 rakwb eng Localization of genes responsible for replication and immunity to colicin A on plasmid ColA-CA31 1983 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary The region containing the origin and regulatory sites for replication as well as the immunity gene (iaa) have been localized on the plasmid ColA-CA31. The region involved in replication functions of ColA can be hybridized with that of ColE1. It is located between 0 and 1 Kb on the plasmid map previously published (Morlon et al. 1982a). A 0.50 Kb HincII fragment of ColA can be weakly hybridized to the ColE1 immunity region. This fragment contains iaa since directed in vitro mutagenesis at an internal restriction site can abolish the immunity to colicin A; however, it does not contain the entire iaa. Knowing the localization of regions involved in autonomous DNA replication and immunity, a mini-ColA plasmid was constructed that contains these two regions. The mini-ColA of 2.8 Kb can be amplified in the presence of chloramphenicol and confers the immunity to transformants. It thus constitutes a useful cloning vector. Expression of ColA and of the various constructed plasmids in the maxicell system suggests that the immunity protein has a molecular weight of about 18–20 Kd. Springer Online Journal Archives 1860-2002 Crozel, Veronica oth Lazdunski, Claude oth Cavard, Danielle oth in Molecular genetics and genomics 1908 192(1983) vom: März, Seite 500-505 (DE-627)NLEJ188991441 (DE-600)1462070-4 1617-4623 nnns volume:192 year:1983 month:03 pages:500-505 extent:6 http://dx.doi.org/10.1007/BF00392196 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 192 1983 3 500-505 6 |
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(DE-627)NLEJ205477070 DE-627 ger DE-627 rakwb eng Localization of genes responsible for replication and immunity to colicin A on plasmid ColA-CA31 1983 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary The region containing the origin and regulatory sites for replication as well as the immunity gene (iaa) have been localized on the plasmid ColA-CA31. The region involved in replication functions of ColA can be hybridized with that of ColE1. It is located between 0 and 1 Kb on the plasmid map previously published (Morlon et al. 1982a). A 0.50 Kb HincII fragment of ColA can be weakly hybridized to the ColE1 immunity region. This fragment contains iaa since directed in vitro mutagenesis at an internal restriction site can abolish the immunity to colicin A; however, it does not contain the entire iaa. Knowing the localization of regions involved in autonomous DNA replication and immunity, a mini-ColA plasmid was constructed that contains these two regions. The mini-ColA of 2.8 Kb can be amplified in the presence of chloramphenicol and confers the immunity to transformants. It thus constitutes a useful cloning vector. Expression of ColA and of the various constructed plasmids in the maxicell system suggests that the immunity protein has a molecular weight of about 18–20 Kd. Springer Online Journal Archives 1860-2002 Crozel, Veronica oth Lazdunski, Claude oth Cavard, Danielle oth in Molecular genetics and genomics 1908 192(1983) vom: März, Seite 500-505 (DE-627)NLEJ188991441 (DE-600)1462070-4 1617-4623 nnns volume:192 year:1983 month:03 pages:500-505 extent:6 http://dx.doi.org/10.1007/BF00392196 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 192 1983 3 500-505 6 |
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(DE-627)NLEJ205477070 DE-627 ger DE-627 rakwb eng Localization of genes responsible for replication and immunity to colicin A on plasmid ColA-CA31 1983 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary The region containing the origin and regulatory sites for replication as well as the immunity gene (iaa) have been localized on the plasmid ColA-CA31. The region involved in replication functions of ColA can be hybridized with that of ColE1. It is located between 0 and 1 Kb on the plasmid map previously published (Morlon et al. 1982a). A 0.50 Kb HincII fragment of ColA can be weakly hybridized to the ColE1 immunity region. This fragment contains iaa since directed in vitro mutagenesis at an internal restriction site can abolish the immunity to colicin A; however, it does not contain the entire iaa. Knowing the localization of regions involved in autonomous DNA replication and immunity, a mini-ColA plasmid was constructed that contains these two regions. The mini-ColA of 2.8 Kb can be amplified in the presence of chloramphenicol and confers the immunity to transformants. It thus constitutes a useful cloning vector. Expression of ColA and of the various constructed plasmids in the maxicell system suggests that the immunity protein has a molecular weight of about 18–20 Kd. Springer Online Journal Archives 1860-2002 Crozel, Veronica oth Lazdunski, Claude oth Cavard, Danielle oth in Molecular genetics and genomics 1908 192(1983) vom: März, Seite 500-505 (DE-627)NLEJ188991441 (DE-600)1462070-4 1617-4623 nnns volume:192 year:1983 month:03 pages:500-505 extent:6 http://dx.doi.org/10.1007/BF00392196 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 192 1983 3 500-505 6 |
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in Molecular genetics and genomics 192(1983) vom: März, Seite 500-505 volume:192 year:1983 month:03 pages:500-505 extent:6 |
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Localization of genes responsible for replication and immunity to colicin A on plasmid ColA-CA31 |
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Localization of genes responsible for replication and immunity to colicin A on plasmid ColA-CA31 |
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Localization of genes responsible for replication and immunity to colicin A on plasmid ColA-CA31 |
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localization of genes responsible for replication and immunity to colicin a on plasmid cola-ca31 |
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Localization of genes responsible for replication and immunity to colicin A on plasmid ColA-CA31 |
| abstract |
Summary The region containing the origin and regulatory sites for replication as well as the immunity gene (iaa) have been localized on the plasmid ColA-CA31. The region involved in replication functions of ColA can be hybridized with that of ColE1. It is located between 0 and 1 Kb on the plasmid map previously published (Morlon et al. 1982a). A 0.50 Kb HincII fragment of ColA can be weakly hybridized to the ColE1 immunity region. This fragment contains iaa since directed in vitro mutagenesis at an internal restriction site can abolish the immunity to colicin A; however, it does not contain the entire iaa. Knowing the localization of regions involved in autonomous DNA replication and immunity, a mini-ColA plasmid was constructed that contains these two regions. The mini-ColA of 2.8 Kb can be amplified in the presence of chloramphenicol and confers the immunity to transformants. It thus constitutes a useful cloning vector. Expression of ColA and of the various constructed plasmids in the maxicell system suggests that the immunity protein has a molecular weight of about 18–20 Kd. |
| abstractGer |
Summary The region containing the origin and regulatory sites for replication as well as the immunity gene (iaa) have been localized on the plasmid ColA-CA31. The region involved in replication functions of ColA can be hybridized with that of ColE1. It is located between 0 and 1 Kb on the plasmid map previously published (Morlon et al. 1982a). A 0.50 Kb HincII fragment of ColA can be weakly hybridized to the ColE1 immunity region. This fragment contains iaa since directed in vitro mutagenesis at an internal restriction site can abolish the immunity to colicin A; however, it does not contain the entire iaa. Knowing the localization of regions involved in autonomous DNA replication and immunity, a mini-ColA plasmid was constructed that contains these two regions. The mini-ColA of 2.8 Kb can be amplified in the presence of chloramphenicol and confers the immunity to transformants. It thus constitutes a useful cloning vector. Expression of ColA and of the various constructed plasmids in the maxicell system suggests that the immunity protein has a molecular weight of about 18–20 Kd. |
| abstract_unstemmed |
Summary The region containing the origin and regulatory sites for replication as well as the immunity gene (iaa) have been localized on the plasmid ColA-CA31. The region involved in replication functions of ColA can be hybridized with that of ColE1. It is located between 0 and 1 Kb on the plasmid map previously published (Morlon et al. 1982a). A 0.50 Kb HincII fragment of ColA can be weakly hybridized to the ColE1 immunity region. This fragment contains iaa since directed in vitro mutagenesis at an internal restriction site can abolish the immunity to colicin A; however, it does not contain the entire iaa. Knowing the localization of regions involved in autonomous DNA replication and immunity, a mini-ColA plasmid was constructed that contains these two regions. The mini-ColA of 2.8 Kb can be amplified in the presence of chloramphenicol and confers the immunity to transformants. It thus constitutes a useful cloning vector. Expression of ColA and of the various constructed plasmids in the maxicell system suggests that the immunity protein has a molecular weight of about 18–20 Kd. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ205477070</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210706175805.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070528s1983 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ205477070</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Localization of genes responsible for replication and immunity to colicin A on plasmid ColA-CA31</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1983</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">6</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Summary The region containing the origin and regulatory sites for replication as well as the immunity gene (iaa) have been localized on the plasmid ColA-CA31. The region involved in replication functions of ColA can be hybridized with that of ColE1. It is located between 0 and 1 Kb on the plasmid map previously published (Morlon et al. 1982a). A 0.50 Kb HincII fragment of ColA can be weakly hybridized to the ColE1 immunity region. This fragment contains iaa since directed in vitro mutagenesis at an internal restriction site can abolish the immunity to colicin A; however, it does not contain the entire iaa. Knowing the localization of regions involved in autonomous DNA replication and immunity, a mini-ColA plasmid was constructed that contains these two regions. The mini-ColA of 2.8 Kb can be amplified in the presence of chloramphenicol and confers the immunity to transformants. It thus constitutes a useful cloning vector. Expression of ColA and of the various constructed plasmids in the maxicell system suggests that the immunity protein has a molecular weight of about 18–20 Kd.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Springer Online Journal Archives 1860-2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Crozel, Veronica</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Lazdunski, Claude</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Cavard, Danielle</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">Molecular genetics and genomics</subfield><subfield code="d">1908</subfield><subfield code="g">192(1983) vom: März, Seite 500-505</subfield><subfield code="w">(DE-627)NLEJ188991441</subfield><subfield code="w">(DE-600)1462070-4</subfield><subfield code="x">1617-4623</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:192</subfield><subfield code="g">year:1983</subfield><subfield code="g">month:03</subfield><subfield code="g">pages:500-505</subfield><subfield code="g">extent:6</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1007/BF00392196</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SOJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">192</subfield><subfield code="j">1983</subfield><subfield code="c">3</subfield><subfield code="h">500-505</subfield><subfield code="g">6</subfield></datafield></record></collection>
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