Quantitative analysis of outward rectifying K+ channel currents in guard cell protoplasts fromVicia faba
Summary A quantitative analysis of the time and voltage dependence of outward-rectifying K+ currents ( $$I_{K^ + .out} $$ ) in guard cells fromVicia faba is described using the whole-cell patch-clamp technique. After step depolarizations from −75 mV to potentials positive to −40 mV, time-dependent o...
Ausführliche Beschreibung
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| Autor*in: |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1989 |
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| Umfang: |
7 |
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| Reproduktion: |
Springer Online Journal Archives 1860-2002 |
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| Übergeordnetes Werk: |
in: The journal of membrane biology - 1969, 107(1989) vom: März, Seite 229-235 |
| Übergeordnetes Werk: |
volume:107 ; year:1989 ; month:03 ; pages:229-235 ; extent:7 |
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NLEJ207034702 |
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| 245 | 1 | 0 | |a Quantitative analysis of outward rectifying K+ channel currents in guard cell protoplasts fromVicia faba |
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| 520 | |a Summary A quantitative analysis of the time and voltage dependence of outward-rectifying K+ currents ( $$I_{K^ + .out} $$ ) in guard cells fromVicia faba is described using the whole-cell patch-clamp technique. After step depolarizations from −75 mV to potentials positive to −40 mV, time-dependent outward currents were produced, which have recently been identified as K+ channel currents. This K+ current was characterized according to its time dependence and its steady-state activation. $$I_{K^ + .out} $$ could be described in terms of a Hodgkin-Huxley type conductance. Activation of the current in time was sigmoid and was well fitted by raising the activation variable to the second power. Deactivating tail currents were single exponentials, which suggests that only one conductance underlies this slow outward K+ current. Rates of channel closing were strongly dependent on the membrane potential, while rates of channel opening showed only limited voltage dependence leading to a highly asymmetric voltage dependence for channel closing and opening. The presented analysis provides a quantitative basis for the understanding of $$I_{K^ + .out} $$ channel gating and $$I_{K^ + .out} $$ channel functions in plant cells. | ||
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(DE-627)NLEJ207034702 DE-627 ger DE-627 rakwb eng Quantitative analysis of outward rectifying K+ channel currents in guard cell protoplasts fromVicia faba 1989 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary A quantitative analysis of the time and voltage dependence of outward-rectifying K+ currents ( $$I_{K^ + .out} $$ ) in guard cells fromVicia faba is described using the whole-cell patch-clamp technique. After step depolarizations from −75 mV to potentials positive to −40 mV, time-dependent outward currents were produced, which have recently been identified as K+ channel currents. This K+ current was characterized according to its time dependence and its steady-state activation. $$I_{K^ + .out} $$ could be described in terms of a Hodgkin-Huxley type conductance. Activation of the current in time was sigmoid and was well fitted by raising the activation variable to the second power. Deactivating tail currents were single exponentials, which suggests that only one conductance underlies this slow outward K+ current. Rates of channel closing were strongly dependent on the membrane potential, while rates of channel opening showed only limited voltage dependence leading to a highly asymmetric voltage dependence for channel closing and opening. The presented analysis provides a quantitative basis for the understanding of $$I_{K^ + .out} $$ channel gating and $$I_{K^ + .out} $$ channel functions in plant cells. Springer Online Journal Archives 1860-2002 Schroeder, Julian I. oth in The journal of membrane biology 1969 107(1989) vom: März, Seite 229-235 (DE-627)NLEJ18899419X (DE-600)1459323-3 1432-1424 nnns volume:107 year:1989 month:03 pages:229-235 extent:7 http://dx.doi.org/10.1007/BF01871938 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 107 1989 3 229-235 7 |
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(DE-627)NLEJ207034702 DE-627 ger DE-627 rakwb eng Quantitative analysis of outward rectifying K+ channel currents in guard cell protoplasts fromVicia faba 1989 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary A quantitative analysis of the time and voltage dependence of outward-rectifying K+ currents ( $$I_{K^ + .out} $$ ) in guard cells fromVicia faba is described using the whole-cell patch-clamp technique. After step depolarizations from −75 mV to potentials positive to −40 mV, time-dependent outward currents were produced, which have recently been identified as K+ channel currents. This K+ current was characterized according to its time dependence and its steady-state activation. $$I_{K^ + .out} $$ could be described in terms of a Hodgkin-Huxley type conductance. Activation of the current in time was sigmoid and was well fitted by raising the activation variable to the second power. Deactivating tail currents were single exponentials, which suggests that only one conductance underlies this slow outward K+ current. Rates of channel closing were strongly dependent on the membrane potential, while rates of channel opening showed only limited voltage dependence leading to a highly asymmetric voltage dependence for channel closing and opening. The presented analysis provides a quantitative basis for the understanding of $$I_{K^ + .out} $$ channel gating and $$I_{K^ + .out} $$ channel functions in plant cells. Springer Online Journal Archives 1860-2002 Schroeder, Julian I. oth in The journal of membrane biology 1969 107(1989) vom: März, Seite 229-235 (DE-627)NLEJ18899419X (DE-600)1459323-3 1432-1424 nnns volume:107 year:1989 month:03 pages:229-235 extent:7 http://dx.doi.org/10.1007/BF01871938 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 107 1989 3 229-235 7 |
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(DE-627)NLEJ207034702 DE-627 ger DE-627 rakwb eng Quantitative analysis of outward rectifying K+ channel currents in guard cell protoplasts fromVicia faba 1989 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary A quantitative analysis of the time and voltage dependence of outward-rectifying K+ currents ( $$I_{K^ + .out} $$ ) in guard cells fromVicia faba is described using the whole-cell patch-clamp technique. After step depolarizations from −75 mV to potentials positive to −40 mV, time-dependent outward currents were produced, which have recently been identified as K+ channel currents. This K+ current was characterized according to its time dependence and its steady-state activation. $$I_{K^ + .out} $$ could be described in terms of a Hodgkin-Huxley type conductance. Activation of the current in time was sigmoid and was well fitted by raising the activation variable to the second power. Deactivating tail currents were single exponentials, which suggests that only one conductance underlies this slow outward K+ current. Rates of channel closing were strongly dependent on the membrane potential, while rates of channel opening showed only limited voltage dependence leading to a highly asymmetric voltage dependence for channel closing and opening. The presented analysis provides a quantitative basis for the understanding of $$I_{K^ + .out} $$ channel gating and $$I_{K^ + .out} $$ channel functions in plant cells. Springer Online Journal Archives 1860-2002 Schroeder, Julian I. oth in The journal of membrane biology 1969 107(1989) vom: März, Seite 229-235 (DE-627)NLEJ18899419X (DE-600)1459323-3 1432-1424 nnns volume:107 year:1989 month:03 pages:229-235 extent:7 http://dx.doi.org/10.1007/BF01871938 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 107 1989 3 229-235 7 |
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(DE-627)NLEJ207034702 DE-627 ger DE-627 rakwb eng Quantitative analysis of outward rectifying K+ channel currents in guard cell protoplasts fromVicia faba 1989 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary A quantitative analysis of the time and voltage dependence of outward-rectifying K+ currents ( $$I_{K^ + .out} $$ ) in guard cells fromVicia faba is described using the whole-cell patch-clamp technique. After step depolarizations from −75 mV to potentials positive to −40 mV, time-dependent outward currents were produced, which have recently been identified as K+ channel currents. This K+ current was characterized according to its time dependence and its steady-state activation. $$I_{K^ + .out} $$ could be described in terms of a Hodgkin-Huxley type conductance. Activation of the current in time was sigmoid and was well fitted by raising the activation variable to the second power. Deactivating tail currents were single exponentials, which suggests that only one conductance underlies this slow outward K+ current. Rates of channel closing were strongly dependent on the membrane potential, while rates of channel opening showed only limited voltage dependence leading to a highly asymmetric voltage dependence for channel closing and opening. The presented analysis provides a quantitative basis for the understanding of $$I_{K^ + .out} $$ channel gating and $$I_{K^ + .out} $$ channel functions in plant cells. Springer Online Journal Archives 1860-2002 Schroeder, Julian I. oth in The journal of membrane biology 1969 107(1989) vom: März, Seite 229-235 (DE-627)NLEJ18899419X (DE-600)1459323-3 1432-1424 nnns volume:107 year:1989 month:03 pages:229-235 extent:7 http://dx.doi.org/10.1007/BF01871938 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 107 1989 3 229-235 7 |
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(DE-627)NLEJ207034702 DE-627 ger DE-627 rakwb eng Quantitative analysis of outward rectifying K+ channel currents in guard cell protoplasts fromVicia faba 1989 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary A quantitative analysis of the time and voltage dependence of outward-rectifying K+ currents ( $$I_{K^ + .out} $$ ) in guard cells fromVicia faba is described using the whole-cell patch-clamp technique. After step depolarizations from −75 mV to potentials positive to −40 mV, time-dependent outward currents were produced, which have recently been identified as K+ channel currents. This K+ current was characterized according to its time dependence and its steady-state activation. $$I_{K^ + .out} $$ could be described in terms of a Hodgkin-Huxley type conductance. Activation of the current in time was sigmoid and was well fitted by raising the activation variable to the second power. Deactivating tail currents were single exponentials, which suggests that only one conductance underlies this slow outward K+ current. Rates of channel closing were strongly dependent on the membrane potential, while rates of channel opening showed only limited voltage dependence leading to a highly asymmetric voltage dependence for channel closing and opening. The presented analysis provides a quantitative basis for the understanding of $$I_{K^ + .out} $$ channel gating and $$I_{K^ + .out} $$ channel functions in plant cells. Springer Online Journal Archives 1860-2002 Schroeder, Julian I. oth in The journal of membrane biology 1969 107(1989) vom: März, Seite 229-235 (DE-627)NLEJ18899419X (DE-600)1459323-3 1432-1424 nnns volume:107 year:1989 month:03 pages:229-235 extent:7 http://dx.doi.org/10.1007/BF01871938 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 107 1989 3 229-235 7 |
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quantitative analysis of outward rectifying k+ channel currents in guard cell protoplasts fromvicia faba |
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Quantitative analysis of outward rectifying K+ channel currents in guard cell protoplasts fromVicia faba |
| abstract |
Summary A quantitative analysis of the time and voltage dependence of outward-rectifying K+ currents ( $$I_{K^ + .out} $$ ) in guard cells fromVicia faba is described using the whole-cell patch-clamp technique. After step depolarizations from −75 mV to potentials positive to −40 mV, time-dependent outward currents were produced, which have recently been identified as K+ channel currents. This K+ current was characterized according to its time dependence and its steady-state activation. $$I_{K^ + .out} $$ could be described in terms of a Hodgkin-Huxley type conductance. Activation of the current in time was sigmoid and was well fitted by raising the activation variable to the second power. Deactivating tail currents were single exponentials, which suggests that only one conductance underlies this slow outward K+ current. Rates of channel closing were strongly dependent on the membrane potential, while rates of channel opening showed only limited voltage dependence leading to a highly asymmetric voltage dependence for channel closing and opening. The presented analysis provides a quantitative basis for the understanding of $$I_{K^ + .out} $$ channel gating and $$I_{K^ + .out} $$ channel functions in plant cells. |
| abstractGer |
Summary A quantitative analysis of the time and voltage dependence of outward-rectifying K+ currents ( $$I_{K^ + .out} $$ ) in guard cells fromVicia faba is described using the whole-cell patch-clamp technique. After step depolarizations from −75 mV to potentials positive to −40 mV, time-dependent outward currents were produced, which have recently been identified as K+ channel currents. This K+ current was characterized according to its time dependence and its steady-state activation. $$I_{K^ + .out} $$ could be described in terms of a Hodgkin-Huxley type conductance. Activation of the current in time was sigmoid and was well fitted by raising the activation variable to the second power. Deactivating tail currents were single exponentials, which suggests that only one conductance underlies this slow outward K+ current. Rates of channel closing were strongly dependent on the membrane potential, while rates of channel opening showed only limited voltage dependence leading to a highly asymmetric voltage dependence for channel closing and opening. The presented analysis provides a quantitative basis for the understanding of $$I_{K^ + .out} $$ channel gating and $$I_{K^ + .out} $$ channel functions in plant cells. |
| abstract_unstemmed |
Summary A quantitative analysis of the time and voltage dependence of outward-rectifying K+ currents ( $$I_{K^ + .out} $$ ) in guard cells fromVicia faba is described using the whole-cell patch-clamp technique. After step depolarizations from −75 mV to potentials positive to −40 mV, time-dependent outward currents were produced, which have recently been identified as K+ channel currents. This K+ current was characterized according to its time dependence and its steady-state activation. $$I_{K^ + .out} $$ could be described in terms of a Hodgkin-Huxley type conductance. Activation of the current in time was sigmoid and was well fitted by raising the activation variable to the second power. Deactivating tail currents were single exponentials, which suggests that only one conductance underlies this slow outward K+ current. Rates of channel closing were strongly dependent on the membrane potential, while rates of channel opening showed only limited voltage dependence leading to a highly asymmetric voltage dependence for channel closing and opening. The presented analysis provides a quantitative basis for the understanding of $$I_{K^ + .out} $$ channel gating and $$I_{K^ + .out} $$ channel functions in plant cells. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ207034702</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210706215914.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070528s1989 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ207034702</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Quantitative analysis of outward rectifying K+ channel currents in guard cell protoplasts fromVicia faba</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1989</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">7</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Summary A quantitative analysis of the time and voltage dependence of outward-rectifying K+ currents ( $$I_{K^ + .out} $$ ) in guard cells fromVicia faba is described using the whole-cell patch-clamp technique. After step depolarizations from −75 mV to potentials positive to −40 mV, time-dependent outward currents were produced, which have recently been identified as K+ channel currents. This K+ current was characterized according to its time dependence and its steady-state activation. $$I_{K^ + .out} $$ could be described in terms of a Hodgkin-Huxley type conductance. Activation of the current in time was sigmoid and was well fitted by raising the activation variable to the second power. Deactivating tail currents were single exponentials, which suggests that only one conductance underlies this slow outward K+ current. Rates of channel closing were strongly dependent on the membrane potential, while rates of channel opening showed only limited voltage dependence leading to a highly asymmetric voltage dependence for channel closing and opening. The presented analysis provides a quantitative basis for the understanding of $$I_{K^ + .out} $$ channel gating and $$I_{K^ + .out} $$ channel functions in plant cells.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Springer Online Journal Archives 1860-2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Schroeder, Julian I.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">The journal of membrane biology</subfield><subfield code="d">1969</subfield><subfield code="g">107(1989) vom: März, Seite 229-235</subfield><subfield code="w">(DE-627)NLEJ18899419X</subfield><subfield code="w">(DE-600)1459323-3</subfield><subfield code="x">1432-1424</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:107</subfield><subfield code="g">year:1989</subfield><subfield code="g">month:03</subfield><subfield code="g">pages:229-235</subfield><subfield code="g">extent:7</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1007/BF01871938</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SOJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">107</subfield><subfield code="j">1989</subfield><subfield code="c">3</subfield><subfield code="h">229-235</subfield><subfield code="g">7</subfield></datafield></record></collection>
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7.401335 |