Cloning, Sequencing, and Function of sanF: A Gene Involved in Nikkomycin Biosynthesis of Streptomyces ansochromogenes
Abstract A 111-bp DNA fragment related to nikkomycin biosynthesis of Streptomyces ansochromogenes 7100 was obtained with the method of reverse genetics. Then, a 2.2-kb DNA fragment was cloned from the DNA library of S. ansochromogenes 7100 by using the 111-bp fragment as a probe. Sequence analysis s...
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2000 |
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| Umfang: |
5 |
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Springer Online Journal Archives 1860-2002 |
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| Übergeordnetes Werk: |
in: Current microbiology - 1981, 41(2000) vom: Mai, Seite 312-316 |
| Übergeordnetes Werk: |
volume:41 ; year:2000 ; month:05 ; pages:312-316 ; extent:5 |
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| 520 | |a Abstract A 111-bp DNA fragment related to nikkomycin biosynthesis of Streptomyces ansochromogenes 7100 was obtained with the method of reverse genetics. Then, a 2.2-kb DNA fragment was cloned from the DNA library of S. ansochromogenes 7100 by using the 111-bp fragment as a probe. Sequence analysis showed that the fragment contains one complete open reading frame (ORF) that encodes a 219-amino acid (aa) protein, and this gene was designated sanF (GenBank Accession No. AF223971). The function of the sanF gene was studied by a strategy of gene disruption, and the resulting sanF mutants lost the ability to synthesize biologically active nikkomycin, indicating that sanF is essential for nikkomycin biosynthesis. | ||
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(DE-627)NLEJ207312567 DE-627 ger DE-627 rakwb eng Cloning, Sequencing, and Function of sanF: A Gene Involved in Nikkomycin Biosynthesis of Streptomyces ansochromogenes 2000 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract A 111-bp DNA fragment related to nikkomycin biosynthesis of Streptomyces ansochromogenes 7100 was obtained with the method of reverse genetics. Then, a 2.2-kb DNA fragment was cloned from the DNA library of S. ansochromogenes 7100 by using the 111-bp fragment as a probe. Sequence analysis showed that the fragment contains one complete open reading frame (ORF) that encodes a 219-amino acid (aa) protein, and this gene was designated sanF (GenBank Accession No. AF223971). The function of the sanF gene was studied by a strategy of gene disruption, and the resulting sanF mutants lost the ability to synthesize biologically active nikkomycin, indicating that sanF is essential for nikkomycin biosynthesis. Springer Online Journal Archives 1860-2002 Chen, Wei oth Zeng, Hongmei oth Tan, Huarong oth in Current microbiology 1981 41(2000) vom: Mai, Seite 312-316 (DE-627)NLEJ188990542 (DE-600)1458987-4 1432-0991 nnns volume:41 year:2000 month:05 pages:312-316 extent:5 http://dx.doi.org/10.1007/s002840010141 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 41 2000 5 312-316 5 |
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(DE-627)NLEJ207312567 DE-627 ger DE-627 rakwb eng Cloning, Sequencing, and Function of sanF: A Gene Involved in Nikkomycin Biosynthesis of Streptomyces ansochromogenes 2000 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract A 111-bp DNA fragment related to nikkomycin biosynthesis of Streptomyces ansochromogenes 7100 was obtained with the method of reverse genetics. Then, a 2.2-kb DNA fragment was cloned from the DNA library of S. ansochromogenes 7100 by using the 111-bp fragment as a probe. Sequence analysis showed that the fragment contains one complete open reading frame (ORF) that encodes a 219-amino acid (aa) protein, and this gene was designated sanF (GenBank Accession No. AF223971). The function of the sanF gene was studied by a strategy of gene disruption, and the resulting sanF mutants lost the ability to synthesize biologically active nikkomycin, indicating that sanF is essential for nikkomycin biosynthesis. Springer Online Journal Archives 1860-2002 Chen, Wei oth Zeng, Hongmei oth Tan, Huarong oth in Current microbiology 1981 41(2000) vom: Mai, Seite 312-316 (DE-627)NLEJ188990542 (DE-600)1458987-4 1432-0991 nnns volume:41 year:2000 month:05 pages:312-316 extent:5 http://dx.doi.org/10.1007/s002840010141 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 41 2000 5 312-316 5 |
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(DE-627)NLEJ207312567 DE-627 ger DE-627 rakwb eng Cloning, Sequencing, and Function of sanF: A Gene Involved in Nikkomycin Biosynthesis of Streptomyces ansochromogenes 2000 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract A 111-bp DNA fragment related to nikkomycin biosynthesis of Streptomyces ansochromogenes 7100 was obtained with the method of reverse genetics. Then, a 2.2-kb DNA fragment was cloned from the DNA library of S. ansochromogenes 7100 by using the 111-bp fragment as a probe. Sequence analysis showed that the fragment contains one complete open reading frame (ORF) that encodes a 219-amino acid (aa) protein, and this gene was designated sanF (GenBank Accession No. AF223971). The function of the sanF gene was studied by a strategy of gene disruption, and the resulting sanF mutants lost the ability to synthesize biologically active nikkomycin, indicating that sanF is essential for nikkomycin biosynthesis. Springer Online Journal Archives 1860-2002 Chen, Wei oth Zeng, Hongmei oth Tan, Huarong oth in Current microbiology 1981 41(2000) vom: Mai, Seite 312-316 (DE-627)NLEJ188990542 (DE-600)1458987-4 1432-0991 nnns volume:41 year:2000 month:05 pages:312-316 extent:5 http://dx.doi.org/10.1007/s002840010141 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 41 2000 5 312-316 5 |
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(DE-627)NLEJ207312567 DE-627 ger DE-627 rakwb eng Cloning, Sequencing, and Function of sanF: A Gene Involved in Nikkomycin Biosynthesis of Streptomyces ansochromogenes 2000 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract A 111-bp DNA fragment related to nikkomycin biosynthesis of Streptomyces ansochromogenes 7100 was obtained with the method of reverse genetics. Then, a 2.2-kb DNA fragment was cloned from the DNA library of S. ansochromogenes 7100 by using the 111-bp fragment as a probe. Sequence analysis showed that the fragment contains one complete open reading frame (ORF) that encodes a 219-amino acid (aa) protein, and this gene was designated sanF (GenBank Accession No. AF223971). The function of the sanF gene was studied by a strategy of gene disruption, and the resulting sanF mutants lost the ability to synthesize biologically active nikkomycin, indicating that sanF is essential for nikkomycin biosynthesis. Springer Online Journal Archives 1860-2002 Chen, Wei oth Zeng, Hongmei oth Tan, Huarong oth in Current microbiology 1981 41(2000) vom: Mai, Seite 312-316 (DE-627)NLEJ188990542 (DE-600)1458987-4 1432-0991 nnns volume:41 year:2000 month:05 pages:312-316 extent:5 http://dx.doi.org/10.1007/s002840010141 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 41 2000 5 312-316 5 |
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(DE-627)NLEJ207312567 DE-627 ger DE-627 rakwb eng Cloning, Sequencing, and Function of sanF: A Gene Involved in Nikkomycin Biosynthesis of Streptomyces ansochromogenes 2000 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract A 111-bp DNA fragment related to nikkomycin biosynthesis of Streptomyces ansochromogenes 7100 was obtained with the method of reverse genetics. Then, a 2.2-kb DNA fragment was cloned from the DNA library of S. ansochromogenes 7100 by using the 111-bp fragment as a probe. Sequence analysis showed that the fragment contains one complete open reading frame (ORF) that encodes a 219-amino acid (aa) protein, and this gene was designated sanF (GenBank Accession No. AF223971). The function of the sanF gene was studied by a strategy of gene disruption, and the resulting sanF mutants lost the ability to synthesize biologically active nikkomycin, indicating that sanF is essential for nikkomycin biosynthesis. Springer Online Journal Archives 1860-2002 Chen, Wei oth Zeng, Hongmei oth Tan, Huarong oth in Current microbiology 1981 41(2000) vom: Mai, Seite 312-316 (DE-627)NLEJ188990542 (DE-600)1458987-4 1432-0991 nnns volume:41 year:2000 month:05 pages:312-316 extent:5 http://dx.doi.org/10.1007/s002840010141 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 41 2000 5 312-316 5 |
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Cloning, Sequencing, and Function of sanF: A Gene Involved in Nikkomycin Biosynthesis of Streptomyces ansochromogenes |
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Abstract A 111-bp DNA fragment related to nikkomycin biosynthesis of Streptomyces ansochromogenes 7100 was obtained with the method of reverse genetics. Then, a 2.2-kb DNA fragment was cloned from the DNA library of S. ansochromogenes 7100 by using the 111-bp fragment as a probe. Sequence analysis showed that the fragment contains one complete open reading frame (ORF) that encodes a 219-amino acid (aa) protein, and this gene was designated sanF (GenBank Accession No. AF223971). The function of the sanF gene was studied by a strategy of gene disruption, and the resulting sanF mutants lost the ability to synthesize biologically active nikkomycin, indicating that sanF is essential for nikkomycin biosynthesis. |
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Abstract A 111-bp DNA fragment related to nikkomycin biosynthesis of Streptomyces ansochromogenes 7100 was obtained with the method of reverse genetics. Then, a 2.2-kb DNA fragment was cloned from the DNA library of S. ansochromogenes 7100 by using the 111-bp fragment as a probe. Sequence analysis showed that the fragment contains one complete open reading frame (ORF) that encodes a 219-amino acid (aa) protein, and this gene was designated sanF (GenBank Accession No. AF223971). The function of the sanF gene was studied by a strategy of gene disruption, and the resulting sanF mutants lost the ability to synthesize biologically active nikkomycin, indicating that sanF is essential for nikkomycin biosynthesis. |
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Abstract A 111-bp DNA fragment related to nikkomycin biosynthesis of Streptomyces ansochromogenes 7100 was obtained with the method of reverse genetics. Then, a 2.2-kb DNA fragment was cloned from the DNA library of S. ansochromogenes 7100 by using the 111-bp fragment as a probe. Sequence analysis showed that the fragment contains one complete open reading frame (ORF) that encodes a 219-amino acid (aa) protein, and this gene was designated sanF (GenBank Accession No. AF223971). The function of the sanF gene was studied by a strategy of gene disruption, and the resulting sanF mutants lost the ability to synthesize biologically active nikkomycin, indicating that sanF is essential for nikkomycin biosynthesis. |
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