The influence of stromal cells on the MTT assay (I)—In vitro chemosensitivity of the tumor and stromal cells to mitomycin C—
Abstract To clarify the influence of stromal cells on the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide assay (MTT assay), a gastric carcinoma cell line (KATO-III) and a human fibroblast cell line (IMR-90) were subjected to a colorimetric assay, in which the chemosensitivity to mi...
Ausführliche Beschreibung
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Englisch |
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1991 |
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4 |
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Springer Online Journal Archives 1860-2002 |
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Übergeordnetes Werk: |
in: Surgery today - 1971, 21(1991) vom: März, Seite 304-307 |
Übergeordnetes Werk: |
volume:21 ; year:1991 ; month:03 ; pages:304-307 ; extent:4 |
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520 | |a Abstract To clarify the influence of stromal cells on the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide assay (MTT assay), a gastric carcinoma cell line (KATO-III) and a human fibroblast cell line (IMR-90) were subjected to a colorimetric assay, in which the chemosensitivity to mitomycin C was assessed in different mixtures of the cell lines. KATO-III was found to be highly sensitive to mitomycin C at 10 μg/ml, whereas IMR-90 was insensitive to mitomycin C at the same concentration. When the mixtures of these two cell lines were tested by the assay, a mixture of more than 25 per cent stromal cells reduced the sensitivity of KATO-III to mitomycin C. This suggested that the stromal cells in fresh surgical specimens might reduce the apparent sensitivity of the tumor cells. | ||
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(DE-627)NLEJ207645515 DE-627 ger DE-627 rakwb eng The influence of stromal cells on the MTT assay (I)—In vitro chemosensitivity of the tumor and stromal cells to mitomycin C— 1991 4 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract To clarify the influence of stromal cells on the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide assay (MTT assay), a gastric carcinoma cell line (KATO-III) and a human fibroblast cell line (IMR-90) were subjected to a colorimetric assay, in which the chemosensitivity to mitomycin C was assessed in different mixtures of the cell lines. KATO-III was found to be highly sensitive to mitomycin C at 10 μg/ml, whereas IMR-90 was insensitive to mitomycin C at the same concentration. When the mixtures of these two cell lines were tested by the assay, a mixture of more than 25 per cent stromal cells reduced the sensitivity of KATO-III to mitomycin C. This suggested that the stromal cells in fresh surgical specimens might reduce the apparent sensitivity of the tumor cells. Springer Online Journal Archives 1860-2002 Suto, Akihiko oth in Surgery today 1971 21(1991) vom: März, Seite 304-307 (DE-627)NLEJ188991352 (DE-600)1463169-6 1436-2813 nnns volume:21 year:1991 month:03 pages:304-307 extent:4 http://dx.doi.org/10.1007/BF02470951 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 21 1991 3 304-307 4 |
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(DE-627)NLEJ207645515 DE-627 ger DE-627 rakwb eng The influence of stromal cells on the MTT assay (I)—In vitro chemosensitivity of the tumor and stromal cells to mitomycin C— 1991 4 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract To clarify the influence of stromal cells on the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide assay (MTT assay), a gastric carcinoma cell line (KATO-III) and a human fibroblast cell line (IMR-90) were subjected to a colorimetric assay, in which the chemosensitivity to mitomycin C was assessed in different mixtures of the cell lines. KATO-III was found to be highly sensitive to mitomycin C at 10 μg/ml, whereas IMR-90 was insensitive to mitomycin C at the same concentration. When the mixtures of these two cell lines were tested by the assay, a mixture of more than 25 per cent stromal cells reduced the sensitivity of KATO-III to mitomycin C. This suggested that the stromal cells in fresh surgical specimens might reduce the apparent sensitivity of the tumor cells. Springer Online Journal Archives 1860-2002 Suto, Akihiko oth in Surgery today 1971 21(1991) vom: März, Seite 304-307 (DE-627)NLEJ188991352 (DE-600)1463169-6 1436-2813 nnns volume:21 year:1991 month:03 pages:304-307 extent:4 http://dx.doi.org/10.1007/BF02470951 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 21 1991 3 304-307 4 |
allfields_unstemmed |
(DE-627)NLEJ207645515 DE-627 ger DE-627 rakwb eng The influence of stromal cells on the MTT assay (I)—In vitro chemosensitivity of the tumor and stromal cells to mitomycin C— 1991 4 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract To clarify the influence of stromal cells on the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide assay (MTT assay), a gastric carcinoma cell line (KATO-III) and a human fibroblast cell line (IMR-90) were subjected to a colorimetric assay, in which the chemosensitivity to mitomycin C was assessed in different mixtures of the cell lines. KATO-III was found to be highly sensitive to mitomycin C at 10 μg/ml, whereas IMR-90 was insensitive to mitomycin C at the same concentration. When the mixtures of these two cell lines were tested by the assay, a mixture of more than 25 per cent stromal cells reduced the sensitivity of KATO-III to mitomycin C. This suggested that the stromal cells in fresh surgical specimens might reduce the apparent sensitivity of the tumor cells. Springer Online Journal Archives 1860-2002 Suto, Akihiko oth in Surgery today 1971 21(1991) vom: März, Seite 304-307 (DE-627)NLEJ188991352 (DE-600)1463169-6 1436-2813 nnns volume:21 year:1991 month:03 pages:304-307 extent:4 http://dx.doi.org/10.1007/BF02470951 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 21 1991 3 304-307 4 |
allfieldsGer |
(DE-627)NLEJ207645515 DE-627 ger DE-627 rakwb eng The influence of stromal cells on the MTT assay (I)—In vitro chemosensitivity of the tumor and stromal cells to mitomycin C— 1991 4 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract To clarify the influence of stromal cells on the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide assay (MTT assay), a gastric carcinoma cell line (KATO-III) and a human fibroblast cell line (IMR-90) were subjected to a colorimetric assay, in which the chemosensitivity to mitomycin C was assessed in different mixtures of the cell lines. KATO-III was found to be highly sensitive to mitomycin C at 10 μg/ml, whereas IMR-90 was insensitive to mitomycin C at the same concentration. When the mixtures of these two cell lines were tested by the assay, a mixture of more than 25 per cent stromal cells reduced the sensitivity of KATO-III to mitomycin C. This suggested that the stromal cells in fresh surgical specimens might reduce the apparent sensitivity of the tumor cells. Springer Online Journal Archives 1860-2002 Suto, Akihiko oth in Surgery today 1971 21(1991) vom: März, Seite 304-307 (DE-627)NLEJ188991352 (DE-600)1463169-6 1436-2813 nnns volume:21 year:1991 month:03 pages:304-307 extent:4 http://dx.doi.org/10.1007/BF02470951 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 21 1991 3 304-307 4 |
allfieldsSound |
(DE-627)NLEJ207645515 DE-627 ger DE-627 rakwb eng The influence of stromal cells on the MTT assay (I)—In vitro chemosensitivity of the tumor and stromal cells to mitomycin C— 1991 4 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract To clarify the influence of stromal cells on the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide assay (MTT assay), a gastric carcinoma cell line (KATO-III) and a human fibroblast cell line (IMR-90) were subjected to a colorimetric assay, in which the chemosensitivity to mitomycin C was assessed in different mixtures of the cell lines. KATO-III was found to be highly sensitive to mitomycin C at 10 μg/ml, whereas IMR-90 was insensitive to mitomycin C at the same concentration. When the mixtures of these two cell lines were tested by the assay, a mixture of more than 25 per cent stromal cells reduced the sensitivity of KATO-III to mitomycin C. This suggested that the stromal cells in fresh surgical specimens might reduce the apparent sensitivity of the tumor cells. Springer Online Journal Archives 1860-2002 Suto, Akihiko oth in Surgery today 1971 21(1991) vom: März, Seite 304-307 (DE-627)NLEJ188991352 (DE-600)1463169-6 1436-2813 nnns volume:21 year:1991 month:03 pages:304-307 extent:4 http://dx.doi.org/10.1007/BF02470951 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 21 1991 3 304-307 4 |
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The influence of stromal cells on the MTT assay (I)—In vitro chemosensitivity of the tumor and stromal cells to mitomycin C— |
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the influence of stromal cells on the mtt assay (i)—in vitro chemosensitivity of the tumor and stromal cells to mitomycin c— |
title_auth |
The influence of stromal cells on the MTT assay (I)—In vitro chemosensitivity of the tumor and stromal cells to mitomycin C— |
abstract |
Abstract To clarify the influence of stromal cells on the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide assay (MTT assay), a gastric carcinoma cell line (KATO-III) and a human fibroblast cell line (IMR-90) were subjected to a colorimetric assay, in which the chemosensitivity to mitomycin C was assessed in different mixtures of the cell lines. KATO-III was found to be highly sensitive to mitomycin C at 10 μg/ml, whereas IMR-90 was insensitive to mitomycin C at the same concentration. When the mixtures of these two cell lines were tested by the assay, a mixture of more than 25 per cent stromal cells reduced the sensitivity of KATO-III to mitomycin C. This suggested that the stromal cells in fresh surgical specimens might reduce the apparent sensitivity of the tumor cells. |
abstractGer |
Abstract To clarify the influence of stromal cells on the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide assay (MTT assay), a gastric carcinoma cell line (KATO-III) and a human fibroblast cell line (IMR-90) were subjected to a colorimetric assay, in which the chemosensitivity to mitomycin C was assessed in different mixtures of the cell lines. KATO-III was found to be highly sensitive to mitomycin C at 10 μg/ml, whereas IMR-90 was insensitive to mitomycin C at the same concentration. When the mixtures of these two cell lines were tested by the assay, a mixture of more than 25 per cent stromal cells reduced the sensitivity of KATO-III to mitomycin C. This suggested that the stromal cells in fresh surgical specimens might reduce the apparent sensitivity of the tumor cells. |
abstract_unstemmed |
Abstract To clarify the influence of stromal cells on the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide assay (MTT assay), a gastric carcinoma cell line (KATO-III) and a human fibroblast cell line (IMR-90) were subjected to a colorimetric assay, in which the chemosensitivity to mitomycin C was assessed in different mixtures of the cell lines. KATO-III was found to be highly sensitive to mitomycin C at 10 μg/ml, whereas IMR-90 was insensitive to mitomycin C at the same concentration. When the mixtures of these two cell lines were tested by the assay, a mixture of more than 25 per cent stromal cells reduced the sensitivity of KATO-III to mitomycin C. This suggested that the stromal cells in fresh surgical specimens might reduce the apparent sensitivity of the tumor cells. |
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