Role of transforming growth factor-β1 during glomerulosclerosis in rats with reduced renal mass
Abstract Background These studies were designed to investigate chronologic changes of transforming growth factor-β1 (TGF-β1) expression during the evolution of glomerulosclerosis in rats after partial nephrectomy. Methods The time course of TGF-β1 expression and its localization at the renal cortex...
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E-Artikel |
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| Sprache: |
Englisch |
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1997 |
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8 |
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Springer Online Journal Archives 1860-2002 |
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in: Clinical and experimental nephrology - 1997, 1(1997) vom: März, Seite 187-194 |
| Übergeordnetes Werk: |
volume:1 ; year:1997 ; month:03 ; pages:187-194 ; extent:8 |
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| 520 | |a Abstract Background These studies were designed to investigate chronologic changes of transforming growth factor-β1 (TGF-β1) expression during the evolution of glomerulosclerosis in rats after partial nephrectomy. Methods The time course of TGF-β1 expression and its localization at the renal cortex was examined immunohistochemically in thirty-six 5/6 nephrectomized (Nx) and 30 sham-operated Wistar rats serially at 3 days and at 1,2,4,8 and 12 weeks after surgery. The extent of glomerulosclerosis was assessed by using the sclerosis index (index range, 0 to 4). Monocyte/macrophage infiltration and type IV collagen were immunohistochemically identified using the monoclonal antibody ED1 and monoclonal anti-type IV collagen antibody, respectively. Results At 12 weeks, the sclerosis index was 1.64±0.20 and 0.06±0.01 (P<0.0001, analysis of variance) in 5/6 nephrectomized and sham-operated rats, respectively. In the nephrectomized rats, the area of positive-TGF-β1 staining increased time-dependently, concomitant with positive results for ED1 and type IV collagen stainings. There was a strong topologic relationship among TGF-β1, ED1, and type IV collagen expression. Northern blot analysis of TGF-β1 messenger ribonucleic acid (mRNA) showed time-dependently augmented expression in nephrectomized, but not in shamoperated, rats (0.8-, 2.2-, 1.6,- and 3.4-fold at 1, 4, 8 and 12 weeks, respectively). Conclusion TGF-β1 plays a pivotal role in the progression of glomerulosclerosis in nephrectomized rats, in association with infiltration of monocytes/macrophages. | ||
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| 700 | 1 | |a Degawa, Noriyuki |4 oth | |
| 700 | 1 | |a Kato, Shuichi |4 oth | |
| 700 | 1 | |a Tomoike, Hitonobu |4 oth | |
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(DE-627)NLEJ207706018 DE-627 ger DE-627 rakwb eng Role of transforming growth factor-β1 during glomerulosclerosis in rats with reduced renal mass 1997 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract Background These studies were designed to investigate chronologic changes of transforming growth factor-β1 (TGF-β1) expression during the evolution of glomerulosclerosis in rats after partial nephrectomy. Methods The time course of TGF-β1 expression and its localization at the renal cortex was examined immunohistochemically in thirty-six 5/6 nephrectomized (Nx) and 30 sham-operated Wistar rats serially at 3 days and at 1,2,4,8 and 12 weeks after surgery. The extent of glomerulosclerosis was assessed by using the sclerosis index (index range, 0 to 4). Monocyte/macrophage infiltration and type IV collagen were immunohistochemically identified using the monoclonal antibody ED1 and monoclonal anti-type IV collagen antibody, respectively. Results At 12 weeks, the sclerosis index was 1.64±0.20 and 0.06±0.01 (P<0.0001, analysis of variance) in 5/6 nephrectomized and sham-operated rats, respectively. In the nephrectomized rats, the area of positive-TGF-β1 staining increased time-dependently, concomitant with positive results for ED1 and type IV collagen stainings. There was a strong topologic relationship among TGF-β1, ED1, and type IV collagen expression. Northern blot analysis of TGF-β1 messenger ribonucleic acid (mRNA) showed time-dependently augmented expression in nephrectomized, but not in shamoperated, rats (0.8-, 2.2-, 1.6,- and 3.4-fold at 1, 4, 8 and 12 weeks, respectively). Conclusion TGF-β1 plays a pivotal role in the progression of glomerulosclerosis in nephrectomized rats, in association with infiltration of monocytes/macrophages. Springer Online Journal Archives 1860-2002 Konta, Tsuneo oth Degawa, Noriyuki oth Kato, Shuichi oth Tomoike, Hitonobu oth in Clinical and experimental nephrology 1997 1(1997) vom: März, Seite 187-194 (DE-627)NLEJ188994696 (DE-600)1499111-1 1437-7799 nnns volume:1 year:1997 month:03 pages:187-194 extent:8 http://dx.doi.org/10.1007/BF02480694 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 1 1997 3 187-194 8 |
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(DE-627)NLEJ207706018 DE-627 ger DE-627 rakwb eng Role of transforming growth factor-β1 during glomerulosclerosis in rats with reduced renal mass 1997 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract Background These studies were designed to investigate chronologic changes of transforming growth factor-β1 (TGF-β1) expression during the evolution of glomerulosclerosis in rats after partial nephrectomy. Methods The time course of TGF-β1 expression and its localization at the renal cortex was examined immunohistochemically in thirty-six 5/6 nephrectomized (Nx) and 30 sham-operated Wistar rats serially at 3 days and at 1,2,4,8 and 12 weeks after surgery. The extent of glomerulosclerosis was assessed by using the sclerosis index (index range, 0 to 4). Monocyte/macrophage infiltration and type IV collagen were immunohistochemically identified using the monoclonal antibody ED1 and monoclonal anti-type IV collagen antibody, respectively. Results At 12 weeks, the sclerosis index was 1.64±0.20 and 0.06±0.01 (P<0.0001, analysis of variance) in 5/6 nephrectomized and sham-operated rats, respectively. In the nephrectomized rats, the area of positive-TGF-β1 staining increased time-dependently, concomitant with positive results for ED1 and type IV collagen stainings. There was a strong topologic relationship among TGF-β1, ED1, and type IV collagen expression. Northern blot analysis of TGF-β1 messenger ribonucleic acid (mRNA) showed time-dependently augmented expression in nephrectomized, but not in shamoperated, rats (0.8-, 2.2-, 1.6,- and 3.4-fold at 1, 4, 8 and 12 weeks, respectively). Conclusion TGF-β1 plays a pivotal role in the progression of glomerulosclerosis in nephrectomized rats, in association with infiltration of monocytes/macrophages. Springer Online Journal Archives 1860-2002 Konta, Tsuneo oth Degawa, Noriyuki oth Kato, Shuichi oth Tomoike, Hitonobu oth in Clinical and experimental nephrology 1997 1(1997) vom: März, Seite 187-194 (DE-627)NLEJ188994696 (DE-600)1499111-1 1437-7799 nnns volume:1 year:1997 month:03 pages:187-194 extent:8 http://dx.doi.org/10.1007/BF02480694 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 1 1997 3 187-194 8 |
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(DE-627)NLEJ207706018 DE-627 ger DE-627 rakwb eng Role of transforming growth factor-β1 during glomerulosclerosis in rats with reduced renal mass 1997 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract Background These studies were designed to investigate chronologic changes of transforming growth factor-β1 (TGF-β1) expression during the evolution of glomerulosclerosis in rats after partial nephrectomy. Methods The time course of TGF-β1 expression and its localization at the renal cortex was examined immunohistochemically in thirty-six 5/6 nephrectomized (Nx) and 30 sham-operated Wistar rats serially at 3 days and at 1,2,4,8 and 12 weeks after surgery. The extent of glomerulosclerosis was assessed by using the sclerosis index (index range, 0 to 4). Monocyte/macrophage infiltration and type IV collagen were immunohistochemically identified using the monoclonal antibody ED1 and monoclonal anti-type IV collagen antibody, respectively. Results At 12 weeks, the sclerosis index was 1.64±0.20 and 0.06±0.01 (P<0.0001, analysis of variance) in 5/6 nephrectomized and sham-operated rats, respectively. In the nephrectomized rats, the area of positive-TGF-β1 staining increased time-dependently, concomitant with positive results for ED1 and type IV collagen stainings. There was a strong topologic relationship among TGF-β1, ED1, and type IV collagen expression. Northern blot analysis of TGF-β1 messenger ribonucleic acid (mRNA) showed time-dependently augmented expression in nephrectomized, but not in shamoperated, rats (0.8-, 2.2-, 1.6,- and 3.4-fold at 1, 4, 8 and 12 weeks, respectively). Conclusion TGF-β1 plays a pivotal role in the progression of glomerulosclerosis in nephrectomized rats, in association with infiltration of monocytes/macrophages. Springer Online Journal Archives 1860-2002 Konta, Tsuneo oth Degawa, Noriyuki oth Kato, Shuichi oth Tomoike, Hitonobu oth in Clinical and experimental nephrology 1997 1(1997) vom: März, Seite 187-194 (DE-627)NLEJ188994696 (DE-600)1499111-1 1437-7799 nnns volume:1 year:1997 month:03 pages:187-194 extent:8 http://dx.doi.org/10.1007/BF02480694 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 1 1997 3 187-194 8 |
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(DE-627)NLEJ207706018 DE-627 ger DE-627 rakwb eng Role of transforming growth factor-β1 during glomerulosclerosis in rats with reduced renal mass 1997 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract Background These studies were designed to investigate chronologic changes of transforming growth factor-β1 (TGF-β1) expression during the evolution of glomerulosclerosis in rats after partial nephrectomy. Methods The time course of TGF-β1 expression and its localization at the renal cortex was examined immunohistochemically in thirty-six 5/6 nephrectomized (Nx) and 30 sham-operated Wistar rats serially at 3 days and at 1,2,4,8 and 12 weeks after surgery. The extent of glomerulosclerosis was assessed by using the sclerosis index (index range, 0 to 4). Monocyte/macrophage infiltration and type IV collagen were immunohistochemically identified using the monoclonal antibody ED1 and monoclonal anti-type IV collagen antibody, respectively. Results At 12 weeks, the sclerosis index was 1.64±0.20 and 0.06±0.01 (P<0.0001, analysis of variance) in 5/6 nephrectomized and sham-operated rats, respectively. In the nephrectomized rats, the area of positive-TGF-β1 staining increased time-dependently, concomitant with positive results for ED1 and type IV collagen stainings. There was a strong topologic relationship among TGF-β1, ED1, and type IV collagen expression. Northern blot analysis of TGF-β1 messenger ribonucleic acid (mRNA) showed time-dependently augmented expression in nephrectomized, but not in shamoperated, rats (0.8-, 2.2-, 1.6,- and 3.4-fold at 1, 4, 8 and 12 weeks, respectively). Conclusion TGF-β1 plays a pivotal role in the progression of glomerulosclerosis in nephrectomized rats, in association with infiltration of monocytes/macrophages. Springer Online Journal Archives 1860-2002 Konta, Tsuneo oth Degawa, Noriyuki oth Kato, Shuichi oth Tomoike, Hitonobu oth in Clinical and experimental nephrology 1997 1(1997) vom: März, Seite 187-194 (DE-627)NLEJ188994696 (DE-600)1499111-1 1437-7799 nnns volume:1 year:1997 month:03 pages:187-194 extent:8 http://dx.doi.org/10.1007/BF02480694 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 1 1997 3 187-194 8 |
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(DE-627)NLEJ207706018 DE-627 ger DE-627 rakwb eng Role of transforming growth factor-β1 during glomerulosclerosis in rats with reduced renal mass 1997 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract Background These studies were designed to investigate chronologic changes of transforming growth factor-β1 (TGF-β1) expression during the evolution of glomerulosclerosis in rats after partial nephrectomy. Methods The time course of TGF-β1 expression and its localization at the renal cortex was examined immunohistochemically in thirty-six 5/6 nephrectomized (Nx) and 30 sham-operated Wistar rats serially at 3 days and at 1,2,4,8 and 12 weeks after surgery. The extent of glomerulosclerosis was assessed by using the sclerosis index (index range, 0 to 4). Monocyte/macrophage infiltration and type IV collagen were immunohistochemically identified using the monoclonal antibody ED1 and monoclonal anti-type IV collagen antibody, respectively. Results At 12 weeks, the sclerosis index was 1.64±0.20 and 0.06±0.01 (P<0.0001, analysis of variance) in 5/6 nephrectomized and sham-operated rats, respectively. In the nephrectomized rats, the area of positive-TGF-β1 staining increased time-dependently, concomitant with positive results for ED1 and type IV collagen stainings. There was a strong topologic relationship among TGF-β1, ED1, and type IV collagen expression. Northern blot analysis of TGF-β1 messenger ribonucleic acid (mRNA) showed time-dependently augmented expression in nephrectomized, but not in shamoperated, rats (0.8-, 2.2-, 1.6,- and 3.4-fold at 1, 4, 8 and 12 weeks, respectively). Conclusion TGF-β1 plays a pivotal role in the progression of glomerulosclerosis in nephrectomized rats, in association with infiltration of monocytes/macrophages. Springer Online Journal Archives 1860-2002 Konta, Tsuneo oth Degawa, Noriyuki oth Kato, Shuichi oth Tomoike, Hitonobu oth in Clinical and experimental nephrology 1997 1(1997) vom: März, Seite 187-194 (DE-627)NLEJ188994696 (DE-600)1499111-1 1437-7799 nnns volume:1 year:1997 month:03 pages:187-194 extent:8 http://dx.doi.org/10.1007/BF02480694 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 1 1997 3 187-194 8 |
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Role of transforming growth factor-β1 during glomerulosclerosis in rats with reduced renal mass |
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Abstract Background These studies were designed to investigate chronologic changes of transforming growth factor-β1 (TGF-β1) expression during the evolution of glomerulosclerosis in rats after partial nephrectomy. Methods The time course of TGF-β1 expression and its localization at the renal cortex was examined immunohistochemically in thirty-six 5/6 nephrectomized (Nx) and 30 sham-operated Wistar rats serially at 3 days and at 1,2,4,8 and 12 weeks after surgery. The extent of glomerulosclerosis was assessed by using the sclerosis index (index range, 0 to 4). Monocyte/macrophage infiltration and type IV collagen were immunohistochemically identified using the monoclonal antibody ED1 and monoclonal anti-type IV collagen antibody, respectively. Results At 12 weeks, the sclerosis index was 1.64±0.20 and 0.06±0.01 (P<0.0001, analysis of variance) in 5/6 nephrectomized and sham-operated rats, respectively. In the nephrectomized rats, the area of positive-TGF-β1 staining increased time-dependently, concomitant with positive results for ED1 and type IV collagen stainings. There was a strong topologic relationship among TGF-β1, ED1, and type IV collagen expression. Northern blot analysis of TGF-β1 messenger ribonucleic acid (mRNA) showed time-dependently augmented expression in nephrectomized, but not in shamoperated, rats (0.8-, 2.2-, 1.6,- and 3.4-fold at 1, 4, 8 and 12 weeks, respectively). Conclusion TGF-β1 plays a pivotal role in the progression of glomerulosclerosis in nephrectomized rats, in association with infiltration of monocytes/macrophages. |
| abstractGer |
Abstract Background These studies were designed to investigate chronologic changes of transforming growth factor-β1 (TGF-β1) expression during the evolution of glomerulosclerosis in rats after partial nephrectomy. Methods The time course of TGF-β1 expression and its localization at the renal cortex was examined immunohistochemically in thirty-six 5/6 nephrectomized (Nx) and 30 sham-operated Wistar rats serially at 3 days and at 1,2,4,8 and 12 weeks after surgery. The extent of glomerulosclerosis was assessed by using the sclerosis index (index range, 0 to 4). Monocyte/macrophage infiltration and type IV collagen were immunohistochemically identified using the monoclonal antibody ED1 and monoclonal anti-type IV collagen antibody, respectively. Results At 12 weeks, the sclerosis index was 1.64±0.20 and 0.06±0.01 (P<0.0001, analysis of variance) in 5/6 nephrectomized and sham-operated rats, respectively. In the nephrectomized rats, the area of positive-TGF-β1 staining increased time-dependently, concomitant with positive results for ED1 and type IV collagen stainings. There was a strong topologic relationship among TGF-β1, ED1, and type IV collagen expression. Northern blot analysis of TGF-β1 messenger ribonucleic acid (mRNA) showed time-dependently augmented expression in nephrectomized, but not in shamoperated, rats (0.8-, 2.2-, 1.6,- and 3.4-fold at 1, 4, 8 and 12 weeks, respectively). Conclusion TGF-β1 plays a pivotal role in the progression of glomerulosclerosis in nephrectomized rats, in association with infiltration of monocytes/macrophages. |
| abstract_unstemmed |
Abstract Background These studies were designed to investigate chronologic changes of transforming growth factor-β1 (TGF-β1) expression during the evolution of glomerulosclerosis in rats after partial nephrectomy. Methods The time course of TGF-β1 expression and its localization at the renal cortex was examined immunohistochemically in thirty-six 5/6 nephrectomized (Nx) and 30 sham-operated Wistar rats serially at 3 days and at 1,2,4,8 and 12 weeks after surgery. The extent of glomerulosclerosis was assessed by using the sclerosis index (index range, 0 to 4). Monocyte/macrophage infiltration and type IV collagen were immunohistochemically identified using the monoclonal antibody ED1 and monoclonal anti-type IV collagen antibody, respectively. Results At 12 weeks, the sclerosis index was 1.64±0.20 and 0.06±0.01 (P<0.0001, analysis of variance) in 5/6 nephrectomized and sham-operated rats, respectively. In the nephrectomized rats, the area of positive-TGF-β1 staining increased time-dependently, concomitant with positive results for ED1 and type IV collagen stainings. There was a strong topologic relationship among TGF-β1, ED1, and type IV collagen expression. Northern blot analysis of TGF-β1 messenger ribonucleic acid (mRNA) showed time-dependently augmented expression in nephrectomized, but not in shamoperated, rats (0.8-, 2.2-, 1.6,- and 3.4-fold at 1, 4, 8 and 12 weeks, respectively). Conclusion TGF-β1 plays a pivotal role in the progression of glomerulosclerosis in nephrectomized rats, in association with infiltration of monocytes/macrophages. |
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Role of transforming growth factor-β1 during glomerulosclerosis in rats with reduced renal mass |
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http://dx.doi.org/10.1007/BF02480694 |
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Konta, Tsuneo Degawa, Noriyuki Kato, Shuichi Tomoike, Hitonobu |
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Konta, Tsuneo Degawa, Noriyuki Kato, Shuichi Tomoike, Hitonobu |
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2024-07-06T10:26:58.628Z |
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