Identification of IPNV-specified components released from productively infected RTG-2 cells following massive cytopathic effect
Summary. Rainbow trout gonad cells (RTG-2) display a dramatic cytopathic effect and lysis following productive infection by infectious pancreatic necrosis virus (IPNV). In this study viruses were efficiently released into the growth medium together with low amounts of the monomeric free form of the...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
|---|
| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
1999 |
|---|
| Umfang: |
15 |
|---|
| Reproduktion: |
Springer Online Journal Archives 1860-2002 |
|---|---|
| Übergeordnetes Werk: |
in: Archives of virology - 1939, 144(1999) vom: Aug., Seite 1487-1501 |
| Übergeordnetes Werk: |
volume:144 ; year:1999 ; month:08 ; pages:1487-1501 ; extent:15 |
| Links: |
|---|
| Katalog-ID: |
NLEJ208251340 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLEJ208251340 | ||
| 003 | DE-627 | ||
| 005 | 20230506085556.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 070528s1999 xx |||||o 00| ||eng c | ||
| 035 | |a (DE-627)NLEJ208251340 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 245 | 1 | 0 | |a Identification of IPNV-specified components released from productively infected RTG-2 cells following massive cytopathic effect |
| 264 | 1 | |c 1999 | |
| 300 | |a 15 | ||
| 336 | |a nicht spezifiziert |b zzz |2 rdacontent | ||
| 337 | |a nicht spezifiziert |b z |2 rdamedia | ||
| 338 | |a nicht spezifiziert |b zu |2 rdacarrier | ||
| 520 | |a Summary. Rainbow trout gonad cells (RTG-2) display a dramatic cytopathic effect and lysis following productive infection by infectious pancreatic necrosis virus (IPNV). In this study viruses were efficiently released into the growth medium together with low amounts of the monomeric free form of the structural protein VP3, heterodimers of VP2-VP3, aggregates of pVP2 and viral RNA associated with VP3. Ribonucleoprotein complexes of RNA-VP3 contained RNA equivalent to at the most 25% of full length viral genomes. Infectivity of material released into the growth medium late in infection was only associated with fully assembled viruses and isolated subviral RNA-VP3 complexes were not infectious. Upon purification of IPNV, viral hexa- and pentagonal particles of approx. 15 nm diameter were occasionally co-purified with the virus and then appeared in large quantities. Similar particle-like structures were seen as substructures of purified viruses that were treated with and partially disintegrated by CsCl of virus isodensity concentration. | ||
| 533 | |f Springer Online Journal Archives 1860-2002 | ||
| 700 | 1 | |a Hjalmarsson, A. |4 oth | |
| 700 | 1 | |a Everitt, E. |4 oth | |
| 773 | 0 | 8 | |i in |t Archives of virology |d 1939 |g 144(1999) vom: Aug., Seite 1487-1501 |w (DE-627)NLEJ18899582X |w (DE-600)1458460-8 |x 1432-8798 |7 nnns |
| 773 | 1 | 8 | |g volume:144 |g year:1999 |g month:08 |g pages:1487-1501 |g extent:15 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1007/s007050050605 |
| 912 | |a GBV_USEFLAG_U | ||
| 912 | |a ZDB-1-SOJ | ||
| 912 | |a GBV_NL_ARTICLE | ||
| 951 | |a AR | ||
| 952 | |d 144 |j 1999 |c 8 |h 1487-1501 |g 15 | ||
| matchkey_str |
article:14328798:1999----::dniiainfpvpcfecmoetrlaefopoutvlifcergclsol |
|---|---|
| hierarchy_sort_str |
1999 |
| publishDate |
1999 |
| allfields |
(DE-627)NLEJ208251340 DE-627 ger DE-627 rakwb eng Identification of IPNV-specified components released from productively infected RTG-2 cells following massive cytopathic effect 1999 15 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary. Rainbow trout gonad cells (RTG-2) display a dramatic cytopathic effect and lysis following productive infection by infectious pancreatic necrosis virus (IPNV). In this study viruses were efficiently released into the growth medium together with low amounts of the monomeric free form of the structural protein VP3, heterodimers of VP2-VP3, aggregates of pVP2 and viral RNA associated with VP3. Ribonucleoprotein complexes of RNA-VP3 contained RNA equivalent to at the most 25% of full length viral genomes. Infectivity of material released into the growth medium late in infection was only associated with fully assembled viruses and isolated subviral RNA-VP3 complexes were not infectious. Upon purification of IPNV, viral hexa- and pentagonal particles of approx. 15 nm diameter were occasionally co-purified with the virus and then appeared in large quantities. Similar particle-like structures were seen as substructures of purified viruses that were treated with and partially disintegrated by CsCl of virus isodensity concentration. Springer Online Journal Archives 1860-2002 Hjalmarsson, A. oth Everitt, E. oth in Archives of virology 1939 144(1999) vom: Aug., Seite 1487-1501 (DE-627)NLEJ18899582X (DE-600)1458460-8 1432-8798 nnns volume:144 year:1999 month:08 pages:1487-1501 extent:15 http://dx.doi.org/10.1007/s007050050605 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 144 1999 8 1487-1501 15 |
| spelling |
(DE-627)NLEJ208251340 DE-627 ger DE-627 rakwb eng Identification of IPNV-specified components released from productively infected RTG-2 cells following massive cytopathic effect 1999 15 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary. Rainbow trout gonad cells (RTG-2) display a dramatic cytopathic effect and lysis following productive infection by infectious pancreatic necrosis virus (IPNV). In this study viruses were efficiently released into the growth medium together with low amounts of the monomeric free form of the structural protein VP3, heterodimers of VP2-VP3, aggregates of pVP2 and viral RNA associated with VP3. Ribonucleoprotein complexes of RNA-VP3 contained RNA equivalent to at the most 25% of full length viral genomes. Infectivity of material released into the growth medium late in infection was only associated with fully assembled viruses and isolated subviral RNA-VP3 complexes were not infectious. Upon purification of IPNV, viral hexa- and pentagonal particles of approx. 15 nm diameter were occasionally co-purified with the virus and then appeared in large quantities. Similar particle-like structures were seen as substructures of purified viruses that were treated with and partially disintegrated by CsCl of virus isodensity concentration. Springer Online Journal Archives 1860-2002 Hjalmarsson, A. oth Everitt, E. oth in Archives of virology 1939 144(1999) vom: Aug., Seite 1487-1501 (DE-627)NLEJ18899582X (DE-600)1458460-8 1432-8798 nnns volume:144 year:1999 month:08 pages:1487-1501 extent:15 http://dx.doi.org/10.1007/s007050050605 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 144 1999 8 1487-1501 15 |
| allfields_unstemmed |
(DE-627)NLEJ208251340 DE-627 ger DE-627 rakwb eng Identification of IPNV-specified components released from productively infected RTG-2 cells following massive cytopathic effect 1999 15 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary. Rainbow trout gonad cells (RTG-2) display a dramatic cytopathic effect and lysis following productive infection by infectious pancreatic necrosis virus (IPNV). In this study viruses were efficiently released into the growth medium together with low amounts of the monomeric free form of the structural protein VP3, heterodimers of VP2-VP3, aggregates of pVP2 and viral RNA associated with VP3. Ribonucleoprotein complexes of RNA-VP3 contained RNA equivalent to at the most 25% of full length viral genomes. Infectivity of material released into the growth medium late in infection was only associated with fully assembled viruses and isolated subviral RNA-VP3 complexes were not infectious. Upon purification of IPNV, viral hexa- and pentagonal particles of approx. 15 nm diameter were occasionally co-purified with the virus and then appeared in large quantities. Similar particle-like structures were seen as substructures of purified viruses that were treated with and partially disintegrated by CsCl of virus isodensity concentration. Springer Online Journal Archives 1860-2002 Hjalmarsson, A. oth Everitt, E. oth in Archives of virology 1939 144(1999) vom: Aug., Seite 1487-1501 (DE-627)NLEJ18899582X (DE-600)1458460-8 1432-8798 nnns volume:144 year:1999 month:08 pages:1487-1501 extent:15 http://dx.doi.org/10.1007/s007050050605 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 144 1999 8 1487-1501 15 |
| allfieldsGer |
(DE-627)NLEJ208251340 DE-627 ger DE-627 rakwb eng Identification of IPNV-specified components released from productively infected RTG-2 cells following massive cytopathic effect 1999 15 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary. Rainbow trout gonad cells (RTG-2) display a dramatic cytopathic effect and lysis following productive infection by infectious pancreatic necrosis virus (IPNV). In this study viruses were efficiently released into the growth medium together with low amounts of the monomeric free form of the structural protein VP3, heterodimers of VP2-VP3, aggregates of pVP2 and viral RNA associated with VP3. Ribonucleoprotein complexes of RNA-VP3 contained RNA equivalent to at the most 25% of full length viral genomes. Infectivity of material released into the growth medium late in infection was only associated with fully assembled viruses and isolated subviral RNA-VP3 complexes were not infectious. Upon purification of IPNV, viral hexa- and pentagonal particles of approx. 15 nm diameter were occasionally co-purified with the virus and then appeared in large quantities. Similar particle-like structures were seen as substructures of purified viruses that were treated with and partially disintegrated by CsCl of virus isodensity concentration. Springer Online Journal Archives 1860-2002 Hjalmarsson, A. oth Everitt, E. oth in Archives of virology 1939 144(1999) vom: Aug., Seite 1487-1501 (DE-627)NLEJ18899582X (DE-600)1458460-8 1432-8798 nnns volume:144 year:1999 month:08 pages:1487-1501 extent:15 http://dx.doi.org/10.1007/s007050050605 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 144 1999 8 1487-1501 15 |
| allfieldsSound |
(DE-627)NLEJ208251340 DE-627 ger DE-627 rakwb eng Identification of IPNV-specified components released from productively infected RTG-2 cells following massive cytopathic effect 1999 15 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary. Rainbow trout gonad cells (RTG-2) display a dramatic cytopathic effect and lysis following productive infection by infectious pancreatic necrosis virus (IPNV). In this study viruses were efficiently released into the growth medium together with low amounts of the monomeric free form of the structural protein VP3, heterodimers of VP2-VP3, aggregates of pVP2 and viral RNA associated with VP3. Ribonucleoprotein complexes of RNA-VP3 contained RNA equivalent to at the most 25% of full length viral genomes. Infectivity of material released into the growth medium late in infection was only associated with fully assembled viruses and isolated subviral RNA-VP3 complexes were not infectious. Upon purification of IPNV, viral hexa- and pentagonal particles of approx. 15 nm diameter were occasionally co-purified with the virus and then appeared in large quantities. Similar particle-like structures were seen as substructures of purified viruses that were treated with and partially disintegrated by CsCl of virus isodensity concentration. Springer Online Journal Archives 1860-2002 Hjalmarsson, A. oth Everitt, E. oth in Archives of virology 1939 144(1999) vom: Aug., Seite 1487-1501 (DE-627)NLEJ18899582X (DE-600)1458460-8 1432-8798 nnns volume:144 year:1999 month:08 pages:1487-1501 extent:15 http://dx.doi.org/10.1007/s007050050605 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 144 1999 8 1487-1501 15 |
| language |
English |
| source |
in Archives of virology 144(1999) vom: Aug., Seite 1487-1501 volume:144 year:1999 month:08 pages:1487-1501 extent:15 |
| sourceStr |
in Archives of virology 144(1999) vom: Aug., Seite 1487-1501 volume:144 year:1999 month:08 pages:1487-1501 extent:15 |
| format_phy_str_mv |
Article |
| institution |
findex.gbv.de |
| isfreeaccess_bool |
false |
| container_title |
Archives of virology |
| authorswithroles_txt_mv |
Hjalmarsson, A. @@oth@@ Everitt, E. @@oth@@ |
| publishDateDaySort_date |
1999-08-01T00:00:00Z |
| hierarchy_top_id |
NLEJ18899582X |
| id |
NLEJ208251340 |
| language_de |
englisch |
| fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ208251340</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230506085556.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070528s1999 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ208251340</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Identification of IPNV-specified components released from productively infected RTG-2 cells following massive cytopathic effect</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1999</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">15</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Summary. Rainbow trout gonad cells (RTG-2) display a dramatic cytopathic effect and lysis following productive infection by infectious pancreatic necrosis virus (IPNV). In this study viruses were efficiently released into the growth medium together with low amounts of the monomeric free form of the structural protein VP3, heterodimers of VP2-VP3, aggregates of pVP2 and viral RNA associated with VP3. Ribonucleoprotein complexes of RNA-VP3 contained RNA equivalent to at the most 25% of full length viral genomes. Infectivity of material released into the growth medium late in infection was only associated with fully assembled viruses and isolated subviral RNA-VP3 complexes were not infectious. Upon purification of IPNV, viral hexa- and pentagonal particles of approx. 15 nm diameter were occasionally co-purified with the virus and then appeared in large quantities. Similar particle-like structures were seen as substructures of purified viruses that were treated with and partially disintegrated by CsCl of virus isodensity concentration.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Springer Online Journal Archives 1860-2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Hjalmarsson, A.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Everitt, E.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">Archives of virology</subfield><subfield code="d">1939</subfield><subfield code="g">144(1999) vom: Aug., Seite 1487-1501</subfield><subfield code="w">(DE-627)NLEJ18899582X</subfield><subfield code="w">(DE-600)1458460-8</subfield><subfield code="x">1432-8798</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:144</subfield><subfield code="g">year:1999</subfield><subfield code="g">month:08</subfield><subfield code="g">pages:1487-1501</subfield><subfield code="g">extent:15</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1007/s007050050605</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SOJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">144</subfield><subfield code="j">1999</subfield><subfield code="c">8</subfield><subfield code="h">1487-1501</subfield><subfield code="g">15</subfield></datafield></record></collection>
|
| series2 |
Springer Online Journal Archives 1860-2002 |
| ppnlink_with_tag_str_mv |
@@773@@(DE-627)NLEJ18899582X |
| format |
electronic Article |
| delete_txt_mv |
keep |
| collection |
NL |
| remote_str |
true |
| illustrated |
Not Illustrated |
| issn |
1432-8798 |
| topic_title |
Identification of IPNV-specified components released from productively infected RTG-2 cells following massive cytopathic effect |
| format_facet |
Elektronische Aufsätze Aufsätze Elektronische Ressource |
| format_main_str_mv |
Text Zeitschrift/Artikel |
| carriertype_str_mv |
zu |
| author2_variant |
a h ah e e ee |
| hierarchy_parent_title |
Archives of virology |
| hierarchy_parent_id |
NLEJ18899582X |
| hierarchy_top_title |
Archives of virology |
| isfreeaccess_txt |
false |
| familylinks_str_mv |
(DE-627)NLEJ18899582X (DE-600)1458460-8 |
| title |
Identification of IPNV-specified components released from productively infected RTG-2 cells following massive cytopathic effect |
| spellingShingle |
Identification of IPNV-specified components released from productively infected RTG-2 cells following massive cytopathic effect |
| ctrlnum |
(DE-627)NLEJ208251340 |
| title_full |
Identification of IPNV-specified components released from productively infected RTG-2 cells following massive cytopathic effect |
| journal |
Archives of virology |
| journalStr |
Archives of virology |
| lang_code |
eng |
| isOA_bool |
false |
| recordtype |
marc |
| publishDateSort |
1999 |
| contenttype_str_mv |
zzz |
| container_start_page |
1487 |
| container_volume |
144 |
| physical |
15 |
| format_se |
Elektronische Aufsätze |
| title_sort |
identification of ipnv-specified components released from productively infected rtg-2 cells following massive cytopathic effect |
| title_auth |
Identification of IPNV-specified components released from productively infected RTG-2 cells following massive cytopathic effect |
| abstract |
Summary. Rainbow trout gonad cells (RTG-2) display a dramatic cytopathic effect and lysis following productive infection by infectious pancreatic necrosis virus (IPNV). In this study viruses were efficiently released into the growth medium together with low amounts of the monomeric free form of the structural protein VP3, heterodimers of VP2-VP3, aggregates of pVP2 and viral RNA associated with VP3. Ribonucleoprotein complexes of RNA-VP3 contained RNA equivalent to at the most 25% of full length viral genomes. Infectivity of material released into the growth medium late in infection was only associated with fully assembled viruses and isolated subviral RNA-VP3 complexes were not infectious. Upon purification of IPNV, viral hexa- and pentagonal particles of approx. 15 nm diameter were occasionally co-purified with the virus and then appeared in large quantities. Similar particle-like structures were seen as substructures of purified viruses that were treated with and partially disintegrated by CsCl of virus isodensity concentration. |
| abstractGer |
Summary. Rainbow trout gonad cells (RTG-2) display a dramatic cytopathic effect and lysis following productive infection by infectious pancreatic necrosis virus (IPNV). In this study viruses were efficiently released into the growth medium together with low amounts of the monomeric free form of the structural protein VP3, heterodimers of VP2-VP3, aggregates of pVP2 and viral RNA associated with VP3. Ribonucleoprotein complexes of RNA-VP3 contained RNA equivalent to at the most 25% of full length viral genomes. Infectivity of material released into the growth medium late in infection was only associated with fully assembled viruses and isolated subviral RNA-VP3 complexes were not infectious. Upon purification of IPNV, viral hexa- and pentagonal particles of approx. 15 nm diameter were occasionally co-purified with the virus and then appeared in large quantities. Similar particle-like structures were seen as substructures of purified viruses that were treated with and partially disintegrated by CsCl of virus isodensity concentration. |
| abstract_unstemmed |
Summary. Rainbow trout gonad cells (RTG-2) display a dramatic cytopathic effect and lysis following productive infection by infectious pancreatic necrosis virus (IPNV). In this study viruses were efficiently released into the growth medium together with low amounts of the monomeric free form of the structural protein VP3, heterodimers of VP2-VP3, aggregates of pVP2 and viral RNA associated with VP3. Ribonucleoprotein complexes of RNA-VP3 contained RNA equivalent to at the most 25% of full length viral genomes. Infectivity of material released into the growth medium late in infection was only associated with fully assembled viruses and isolated subviral RNA-VP3 complexes were not infectious. Upon purification of IPNV, viral hexa- and pentagonal particles of approx. 15 nm diameter were occasionally co-purified with the virus and then appeared in large quantities. Similar particle-like structures were seen as substructures of purified viruses that were treated with and partially disintegrated by CsCl of virus isodensity concentration. |
| collection_details |
GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE |
| title_short |
Identification of IPNV-specified components released from productively infected RTG-2 cells following massive cytopathic effect |
| url |
http://dx.doi.org/10.1007/s007050050605 |
| remote_bool |
true |
| author2 |
Hjalmarsson, A. Everitt, E. |
| author2Str |
Hjalmarsson, A. Everitt, E. |
| ppnlink |
NLEJ18899582X |
| mediatype_str_mv |
z |
| isOA_txt |
false |
| hochschulschrift_bool |
false |
| author2_role |
oth oth |
| up_date |
2024-07-06T11:39:19.115Z |
| _version_ |
1803829598346018816 |
| fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ208251340</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230506085556.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070528s1999 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ208251340</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Identification of IPNV-specified components released from productively infected RTG-2 cells following massive cytopathic effect</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1999</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">15</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Summary. Rainbow trout gonad cells (RTG-2) display a dramatic cytopathic effect and lysis following productive infection by infectious pancreatic necrosis virus (IPNV). In this study viruses were efficiently released into the growth medium together with low amounts of the monomeric free form of the structural protein VP3, heterodimers of VP2-VP3, aggregates of pVP2 and viral RNA associated with VP3. Ribonucleoprotein complexes of RNA-VP3 contained RNA equivalent to at the most 25% of full length viral genomes. Infectivity of material released into the growth medium late in infection was only associated with fully assembled viruses and isolated subviral RNA-VP3 complexes were not infectious. Upon purification of IPNV, viral hexa- and pentagonal particles of approx. 15 nm diameter were occasionally co-purified with the virus and then appeared in large quantities. Similar particle-like structures were seen as substructures of purified viruses that were treated with and partially disintegrated by CsCl of virus isodensity concentration.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Springer Online Journal Archives 1860-2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Hjalmarsson, A.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Everitt, E.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">Archives of virology</subfield><subfield code="d">1939</subfield><subfield code="g">144(1999) vom: Aug., Seite 1487-1501</subfield><subfield code="w">(DE-627)NLEJ18899582X</subfield><subfield code="w">(DE-600)1458460-8</subfield><subfield code="x">1432-8798</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:144</subfield><subfield code="g">year:1999</subfield><subfield code="g">month:08</subfield><subfield code="g">pages:1487-1501</subfield><subfield code="g">extent:15</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1007/s007050050605</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SOJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">144</subfield><subfield code="j">1999</subfield><subfield code="c">8</subfield><subfield code="h">1487-1501</subfield><subfield code="g">15</subfield></datafield></record></collection>
|
| score |
7.4024754 |