Glass microelectrode measurements of sieve tube membrane potentials in internode discs and petiole strips of tomato (Solanum lycopersicum L.)
Summary In tissue slices of tomato (Solanum lycopersicum L.) sieve tube membrane potentials (Em) were measured by use of glass microelectrodes. In internode discs, the potential differences (pd) of phloem cells near the cut surface fell into two distinct categories with average values of −66 and −10...
Ausführliche Beschreibung
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| Autor*in: |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1989 |
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| Umfang: |
11 |
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| Reproduktion: |
Springer Online Journal Archives 1860-2002 |
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| Übergeordnetes Werk: |
in: Protoplasma - 1926, 149(1989) vom: Feb./März, Seite 144-154 |
| Übergeordnetes Werk: |
volume:149 ; year:1989 ; month:02/03 ; pages:144-154 ; extent:11 |
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NLEJ208453040 |
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| 245 | 1 | 0 | |a Glass microelectrode measurements of sieve tube membrane potentials in internode discs and petiole strips of tomato (Solanum lycopersicum L.) |
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| 520 | |a Summary In tissue slices of tomato (Solanum lycopersicum L.) sieve tube membrane potentials (Em) were measured by use of glass microelectrodes. In internode discs, the potential differences (pd) of phloem cells near the cut surface fell into two distinct categories with average values of −66 and −109 mV. More distant from the cut surface the values decreased to averages of −71 and −140 mV. These pds were associated with phloem parenchyma cells and sieve tube/companion cell complexes, respectively. In petiole strips, pds were recorded from cells which were identified by iontophoretic injection of fluorescent dye. Averages in two different bathing media, were −140/−146mV, −149/−152mV, and −70/−68mV for sieve tubes, companion cells, and phloem parenchyma cells, respectively. The membrane potentials recorded from sieve tubes were transiently reduced upon sucrose addition. Reduction by CCCP and KCN was more permanent. Sieve tube Ems recovered more slowly from potassium than from sucrose-induced depolarizations. Light/ dark (L/D) responses were minute (±3 mV). The limitations of the present experimentation are evaluated with special reference to the question as whether the recorded Ems represent sieve tube membrane potentials occurring in the intact plant. | ||
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(DE-627)NLEJ208453040 DE-627 ger DE-627 rakwb eng Glass microelectrode measurements of sieve tube membrane potentials in internode discs and petiole strips of tomato (Solanum lycopersicum L.) 1989 11 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary In tissue slices of tomato (Solanum lycopersicum L.) sieve tube membrane potentials (Em) were measured by use of glass microelectrodes. In internode discs, the potential differences (pd) of phloem cells near the cut surface fell into two distinct categories with average values of −66 and −109 mV. More distant from the cut surface the values decreased to averages of −71 and −140 mV. These pds were associated with phloem parenchyma cells and sieve tube/companion cell complexes, respectively. In petiole strips, pds were recorded from cells which were identified by iontophoretic injection of fluorescent dye. Averages in two different bathing media, were −140/−146mV, −149/−152mV, and −70/−68mV for sieve tubes, companion cells, and phloem parenchyma cells, respectively. The membrane potentials recorded from sieve tubes were transiently reduced upon sucrose addition. Reduction by CCCP and KCN was more permanent. Sieve tube Ems recovered more slowly from potassium than from sucrose-induced depolarizations. Light/ dark (L/D) responses were minute (±3 mV). The limitations of the present experimentation are evaluated with special reference to the question as whether the recorded Ems represent sieve tube membrane potentials occurring in the intact plant. Springer Online Journal Archives 1860-2002 Schoot, C. oth Bel, A. J. E. oth in Protoplasma 1926 149(1989) vom: Feb./März, Seite 144-154 (DE-627)NLEJ188989579 (DE-600)1463033-3 1615-6102 nnns volume:149 year:1989 month:02/03 pages:144-154 extent:11 http://dx.doi.org/10.1007/BF01322986 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 149 1989 2/3 144-154 11 |
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(DE-627)NLEJ208453040 DE-627 ger DE-627 rakwb eng Glass microelectrode measurements of sieve tube membrane potentials in internode discs and petiole strips of tomato (Solanum lycopersicum L.) 1989 11 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary In tissue slices of tomato (Solanum lycopersicum L.) sieve tube membrane potentials (Em) were measured by use of glass microelectrodes. In internode discs, the potential differences (pd) of phloem cells near the cut surface fell into two distinct categories with average values of −66 and −109 mV. More distant from the cut surface the values decreased to averages of −71 and −140 mV. These pds were associated with phloem parenchyma cells and sieve tube/companion cell complexes, respectively. In petiole strips, pds were recorded from cells which were identified by iontophoretic injection of fluorescent dye. Averages in two different bathing media, were −140/−146mV, −149/−152mV, and −70/−68mV for sieve tubes, companion cells, and phloem parenchyma cells, respectively. The membrane potentials recorded from sieve tubes were transiently reduced upon sucrose addition. Reduction by CCCP and KCN was more permanent. Sieve tube Ems recovered more slowly from potassium than from sucrose-induced depolarizations. Light/ dark (L/D) responses were minute (±3 mV). The limitations of the present experimentation are evaluated with special reference to the question as whether the recorded Ems represent sieve tube membrane potentials occurring in the intact plant. Springer Online Journal Archives 1860-2002 Schoot, C. oth Bel, A. J. E. oth in Protoplasma 1926 149(1989) vom: Feb./März, Seite 144-154 (DE-627)NLEJ188989579 (DE-600)1463033-3 1615-6102 nnns volume:149 year:1989 month:02/03 pages:144-154 extent:11 http://dx.doi.org/10.1007/BF01322986 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 149 1989 2/3 144-154 11 |
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(DE-627)NLEJ208453040 DE-627 ger DE-627 rakwb eng Glass microelectrode measurements of sieve tube membrane potentials in internode discs and petiole strips of tomato (Solanum lycopersicum L.) 1989 11 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary In tissue slices of tomato (Solanum lycopersicum L.) sieve tube membrane potentials (Em) were measured by use of glass microelectrodes. In internode discs, the potential differences (pd) of phloem cells near the cut surface fell into two distinct categories with average values of −66 and −109 mV. More distant from the cut surface the values decreased to averages of −71 and −140 mV. These pds were associated with phloem parenchyma cells and sieve tube/companion cell complexes, respectively. In petiole strips, pds were recorded from cells which were identified by iontophoretic injection of fluorescent dye. Averages in two different bathing media, were −140/−146mV, −149/−152mV, and −70/−68mV for sieve tubes, companion cells, and phloem parenchyma cells, respectively. The membrane potentials recorded from sieve tubes were transiently reduced upon sucrose addition. Reduction by CCCP and KCN was more permanent. Sieve tube Ems recovered more slowly from potassium than from sucrose-induced depolarizations. Light/ dark (L/D) responses were minute (±3 mV). The limitations of the present experimentation are evaluated with special reference to the question as whether the recorded Ems represent sieve tube membrane potentials occurring in the intact plant. Springer Online Journal Archives 1860-2002 Schoot, C. oth Bel, A. J. E. oth in Protoplasma 1926 149(1989) vom: Feb./März, Seite 144-154 (DE-627)NLEJ188989579 (DE-600)1463033-3 1615-6102 nnns volume:149 year:1989 month:02/03 pages:144-154 extent:11 http://dx.doi.org/10.1007/BF01322986 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 149 1989 2/3 144-154 11 |
| allfieldsGer |
(DE-627)NLEJ208453040 DE-627 ger DE-627 rakwb eng Glass microelectrode measurements of sieve tube membrane potentials in internode discs and petiole strips of tomato (Solanum lycopersicum L.) 1989 11 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary In tissue slices of tomato (Solanum lycopersicum L.) sieve tube membrane potentials (Em) were measured by use of glass microelectrodes. In internode discs, the potential differences (pd) of phloem cells near the cut surface fell into two distinct categories with average values of −66 and −109 mV. More distant from the cut surface the values decreased to averages of −71 and −140 mV. These pds were associated with phloem parenchyma cells and sieve tube/companion cell complexes, respectively. In petiole strips, pds were recorded from cells which were identified by iontophoretic injection of fluorescent dye. Averages in two different bathing media, were −140/−146mV, −149/−152mV, and −70/−68mV for sieve tubes, companion cells, and phloem parenchyma cells, respectively. The membrane potentials recorded from sieve tubes were transiently reduced upon sucrose addition. Reduction by CCCP and KCN was more permanent. Sieve tube Ems recovered more slowly from potassium than from sucrose-induced depolarizations. Light/ dark (L/D) responses were minute (±3 mV). The limitations of the present experimentation are evaluated with special reference to the question as whether the recorded Ems represent sieve tube membrane potentials occurring in the intact plant. Springer Online Journal Archives 1860-2002 Schoot, C. oth Bel, A. J. E. oth in Protoplasma 1926 149(1989) vom: Feb./März, Seite 144-154 (DE-627)NLEJ188989579 (DE-600)1463033-3 1615-6102 nnns volume:149 year:1989 month:02/03 pages:144-154 extent:11 http://dx.doi.org/10.1007/BF01322986 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 149 1989 2/3 144-154 11 |
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(DE-627)NLEJ208453040 DE-627 ger DE-627 rakwb eng Glass microelectrode measurements of sieve tube membrane potentials in internode discs and petiole strips of tomato (Solanum lycopersicum L.) 1989 11 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary In tissue slices of tomato (Solanum lycopersicum L.) sieve tube membrane potentials (Em) were measured by use of glass microelectrodes. In internode discs, the potential differences (pd) of phloem cells near the cut surface fell into two distinct categories with average values of −66 and −109 mV. More distant from the cut surface the values decreased to averages of −71 and −140 mV. These pds were associated with phloem parenchyma cells and sieve tube/companion cell complexes, respectively. In petiole strips, pds were recorded from cells which were identified by iontophoretic injection of fluorescent dye. Averages in two different bathing media, were −140/−146mV, −149/−152mV, and −70/−68mV for sieve tubes, companion cells, and phloem parenchyma cells, respectively. The membrane potentials recorded from sieve tubes were transiently reduced upon sucrose addition. Reduction by CCCP and KCN was more permanent. Sieve tube Ems recovered more slowly from potassium than from sucrose-induced depolarizations. Light/ dark (L/D) responses were minute (±3 mV). The limitations of the present experimentation are evaluated with special reference to the question as whether the recorded Ems represent sieve tube membrane potentials occurring in the intact plant. Springer Online Journal Archives 1860-2002 Schoot, C. oth Bel, A. J. E. oth in Protoplasma 1926 149(1989) vom: Feb./März, Seite 144-154 (DE-627)NLEJ188989579 (DE-600)1463033-3 1615-6102 nnns volume:149 year:1989 month:02/03 pages:144-154 extent:11 http://dx.doi.org/10.1007/BF01322986 GBV_USEFLAG_U ZDB-1-SOJ GBV_NL_ARTICLE AR 149 1989 2/3 144-154 11 |
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Glass microelectrode measurements of sieve tube membrane potentials in internode discs and petiole strips of tomato (Solanum lycopersicum L.) |
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Glass microelectrode measurements of sieve tube membrane potentials in internode discs and petiole strips of tomato (Solanum lycopersicum L.) |
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glass microelectrode measurements of sieve tube membrane potentials in internode discs and petiole strips of tomato (solanum lycopersicum l.) |
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Glass microelectrode measurements of sieve tube membrane potentials in internode discs and petiole strips of tomato (Solanum lycopersicum L.) |
| abstract |
Summary In tissue slices of tomato (Solanum lycopersicum L.) sieve tube membrane potentials (Em) were measured by use of glass microelectrodes. In internode discs, the potential differences (pd) of phloem cells near the cut surface fell into two distinct categories with average values of −66 and −109 mV. More distant from the cut surface the values decreased to averages of −71 and −140 mV. These pds were associated with phloem parenchyma cells and sieve tube/companion cell complexes, respectively. In petiole strips, pds were recorded from cells which were identified by iontophoretic injection of fluorescent dye. Averages in two different bathing media, were −140/−146mV, −149/−152mV, and −70/−68mV for sieve tubes, companion cells, and phloem parenchyma cells, respectively. The membrane potentials recorded from sieve tubes were transiently reduced upon sucrose addition. Reduction by CCCP and KCN was more permanent. Sieve tube Ems recovered more slowly from potassium than from sucrose-induced depolarizations. Light/ dark (L/D) responses were minute (±3 mV). The limitations of the present experimentation are evaluated with special reference to the question as whether the recorded Ems represent sieve tube membrane potentials occurring in the intact plant. |
| abstractGer |
Summary In tissue slices of tomato (Solanum lycopersicum L.) sieve tube membrane potentials (Em) were measured by use of glass microelectrodes. In internode discs, the potential differences (pd) of phloem cells near the cut surface fell into two distinct categories with average values of −66 and −109 mV. More distant from the cut surface the values decreased to averages of −71 and −140 mV. These pds were associated with phloem parenchyma cells and sieve tube/companion cell complexes, respectively. In petiole strips, pds were recorded from cells which were identified by iontophoretic injection of fluorescent dye. Averages in two different bathing media, were −140/−146mV, −149/−152mV, and −70/−68mV for sieve tubes, companion cells, and phloem parenchyma cells, respectively. The membrane potentials recorded from sieve tubes were transiently reduced upon sucrose addition. Reduction by CCCP and KCN was more permanent. Sieve tube Ems recovered more slowly from potassium than from sucrose-induced depolarizations. Light/ dark (L/D) responses were minute (±3 mV). The limitations of the present experimentation are evaluated with special reference to the question as whether the recorded Ems represent sieve tube membrane potentials occurring in the intact plant. |
| abstract_unstemmed |
Summary In tissue slices of tomato (Solanum lycopersicum L.) sieve tube membrane potentials (Em) were measured by use of glass microelectrodes. In internode discs, the potential differences (pd) of phloem cells near the cut surface fell into two distinct categories with average values of −66 and −109 mV. More distant from the cut surface the values decreased to averages of −71 and −140 mV. These pds were associated with phloem parenchyma cells and sieve tube/companion cell complexes, respectively. In petiole strips, pds were recorded from cells which were identified by iontophoretic injection of fluorescent dye. Averages in two different bathing media, were −140/−146mV, −149/−152mV, and −70/−68mV for sieve tubes, companion cells, and phloem parenchyma cells, respectively. The membrane potentials recorded from sieve tubes were transiently reduced upon sucrose addition. Reduction by CCCP and KCN was more permanent. Sieve tube Ems recovered more slowly from potassium than from sucrose-induced depolarizations. Light/ dark (L/D) responses were minute (±3 mV). The limitations of the present experimentation are evaluated with special reference to the question as whether the recorded Ems represent sieve tube membrane potentials occurring in the intact plant. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ208453040</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210707014514.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070528s1989 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ208453040</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Glass microelectrode measurements of sieve tube membrane potentials in internode discs and petiole strips of tomato (Solanum lycopersicum L.)</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1989</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">11</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Summary In tissue slices of tomato (Solanum lycopersicum L.) sieve tube membrane potentials (Em) were measured by use of glass microelectrodes. In internode discs, the potential differences (pd) of phloem cells near the cut surface fell into two distinct categories with average values of −66 and −109 mV. More distant from the cut surface the values decreased to averages of −71 and −140 mV. These pds were associated with phloem parenchyma cells and sieve tube/companion cell complexes, respectively. In petiole strips, pds were recorded from cells which were identified by iontophoretic injection of fluorescent dye. Averages in two different bathing media, were −140/−146mV, −149/−152mV, and −70/−68mV for sieve tubes, companion cells, and phloem parenchyma cells, respectively. The membrane potentials recorded from sieve tubes were transiently reduced upon sucrose addition. Reduction by CCCP and KCN was more permanent. Sieve tube Ems recovered more slowly from potassium than from sucrose-induced depolarizations. Light/ dark (L/D) responses were minute (±3 mV). The limitations of the present experimentation are evaluated with special reference to the question as whether the recorded Ems represent sieve tube membrane potentials occurring in the intact plant.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Springer Online Journal Archives 1860-2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Schoot, C.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Bel, A. J. E.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">Protoplasma</subfield><subfield code="d">1926</subfield><subfield code="g">149(1989) vom: Feb./März, Seite 144-154</subfield><subfield code="w">(DE-627)NLEJ188989579</subfield><subfield code="w">(DE-600)1463033-3</subfield><subfield code="x">1615-6102</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:149</subfield><subfield code="g">year:1989</subfield><subfield code="g">month:02/03</subfield><subfield code="g">pages:144-154</subfield><subfield code="g">extent:11</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1007/BF01322986</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SOJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">149</subfield><subfield code="j">1989</subfield><subfield code="c">2/3</subfield><subfield code="h">144-154</subfield><subfield code="g">11</subfield></datafield></record></collection>
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