Isolation and amplification of DNA from turmeric powder
An efficient protocol for the isolation of high molecular weight DNA from dry powdered samples of turmeric including market samples is described which will help in PCR based detection of adulteration in marketed turmeric powders. The method involves a modified CTAB (3 per cent) procedure with 2?M Na...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2004 |
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| Schlagwörter: |
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| Umfang: |
6 |
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| Reproduktion: |
Emerald Fulltext Archive Database 1994-2005 |
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| Übergeordnetes Werk: |
In: British food journal - Bradford : Emerald, 1899, 106(2004), 9, Seite 673-678 |
| Übergeordnetes Werk: |
volume:106 ; year:2004 ; number:9 ; pages:673-678 ; extent:6 |
| Links: |
|---|
| DOI / URN: |
10.1108/00070700410558201 |
|---|
| Katalog-ID: |
NLEJ219608237 |
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| 245 | 1 | 0 | |a Isolation and amplification of DNA from turmeric powder |
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| 520 | |a An efficient protocol for the isolation of high molecular weight DNA from dry powdered samples of turmeric including market samples is described which will help in PCR based detection of adulteration in marketed turmeric powders. The method involves a modified CTAB (3 per cent) procedure with 2?M NaCl, 0.3 per cent ß-mercaptoethanol coupled with purification of DNA in 30 per cent polyethylene glycol (8000). The yield of the DNA obtained from the samples varied from 2 to 4?µg/g tissue. The DNA obtained from the five different samples were consistently amplifiable (RAPD primers). | ||
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| 700 | 1 | |a Remya, R. |4 oth | |
| 700 | 1 | |a Syamkumar, S. |4 oth | |
| 700 | 1 | |a Sasikumar, B. |4 oth | |
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10.1108/00070700410558201 doi (DE-627)NLEJ219608237 DE-627 ger DE-627 rakwb eng XA-GB Isolation and amplification of DNA from turmeric powder 2004 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier An efficient protocol for the isolation of high molecular weight DNA from dry powdered samples of turmeric including market samples is described which will help in PCR based detection of adulteration in marketed turmeric powders. The method involves a modified CTAB (3 per cent) procedure with 2?M NaCl, 0.3 per cent ß-mercaptoethanol coupled with purification of DNA in 30 per cent polyethylene glycol (8000). The yield of the DNA obtained from the samples varied from 2 to 4?µg/g tissue. The DNA obtained from the five different samples were consistently amplifiable (RAPD primers). Emerald Fulltext Archive Database 1994-2005 Food additives Food testing Remya, R. oth Syamkumar, S. oth Sasikumar, B. oth In British food journal Bradford : Emerald, 1899 106(2004), 9, Seite 673-678 Online-Ressource (DE-627)NLEJ219579555 (DE-600)2027461-0 nnns volume:106 year:2004 number:9 pages:673-678 extent:6 http://dx.doi.org/10.1108/00070700410558201 GBV_USEFLAG_U ZDB-1-EFD GBV_NL_ARTICLE AR 106 2004 9 673-678 6 |
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10.1108/00070700410558201 doi (DE-627)NLEJ219608237 DE-627 ger DE-627 rakwb eng XA-GB Isolation and amplification of DNA from turmeric powder 2004 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier An efficient protocol for the isolation of high molecular weight DNA from dry powdered samples of turmeric including market samples is described which will help in PCR based detection of adulteration in marketed turmeric powders. The method involves a modified CTAB (3 per cent) procedure with 2?M NaCl, 0.3 per cent ß-mercaptoethanol coupled with purification of DNA in 30 per cent polyethylene glycol (8000). The yield of the DNA obtained from the samples varied from 2 to 4?µg/g tissue. The DNA obtained from the five different samples were consistently amplifiable (RAPD primers). Emerald Fulltext Archive Database 1994-2005 Food additives Food testing Remya, R. oth Syamkumar, S. oth Sasikumar, B. oth In British food journal Bradford : Emerald, 1899 106(2004), 9, Seite 673-678 Online-Ressource (DE-627)NLEJ219579555 (DE-600)2027461-0 nnns volume:106 year:2004 number:9 pages:673-678 extent:6 http://dx.doi.org/10.1108/00070700410558201 GBV_USEFLAG_U ZDB-1-EFD GBV_NL_ARTICLE AR 106 2004 9 673-678 6 |
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10.1108/00070700410558201 doi (DE-627)NLEJ219608237 DE-627 ger DE-627 rakwb eng XA-GB Isolation and amplification of DNA from turmeric powder 2004 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier An efficient protocol for the isolation of high molecular weight DNA from dry powdered samples of turmeric including market samples is described which will help in PCR based detection of adulteration in marketed turmeric powders. The method involves a modified CTAB (3 per cent) procedure with 2?M NaCl, 0.3 per cent ß-mercaptoethanol coupled with purification of DNA in 30 per cent polyethylene glycol (8000). The yield of the DNA obtained from the samples varied from 2 to 4?µg/g tissue. The DNA obtained from the five different samples were consistently amplifiable (RAPD primers). Emerald Fulltext Archive Database 1994-2005 Food additives Food testing Remya, R. oth Syamkumar, S. oth Sasikumar, B. oth In British food journal Bradford : Emerald, 1899 106(2004), 9, Seite 673-678 Online-Ressource (DE-627)NLEJ219579555 (DE-600)2027461-0 nnns volume:106 year:2004 number:9 pages:673-678 extent:6 http://dx.doi.org/10.1108/00070700410558201 GBV_USEFLAG_U ZDB-1-EFD GBV_NL_ARTICLE AR 106 2004 9 673-678 6 |
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10.1108/00070700410558201 doi (DE-627)NLEJ219608237 DE-627 ger DE-627 rakwb eng XA-GB Isolation and amplification of DNA from turmeric powder 2004 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier An efficient protocol for the isolation of high molecular weight DNA from dry powdered samples of turmeric including market samples is described which will help in PCR based detection of adulteration in marketed turmeric powders. The method involves a modified CTAB (3 per cent) procedure with 2?M NaCl, 0.3 per cent ß-mercaptoethanol coupled with purification of DNA in 30 per cent polyethylene glycol (8000). The yield of the DNA obtained from the samples varied from 2 to 4?µg/g tissue. The DNA obtained from the five different samples were consistently amplifiable (RAPD primers). Emerald Fulltext Archive Database 1994-2005 Food additives Food testing Remya, R. oth Syamkumar, S. oth Sasikumar, B. oth In British food journal Bradford : Emerald, 1899 106(2004), 9, Seite 673-678 Online-Ressource (DE-627)NLEJ219579555 (DE-600)2027461-0 nnns volume:106 year:2004 number:9 pages:673-678 extent:6 http://dx.doi.org/10.1108/00070700410558201 GBV_USEFLAG_U ZDB-1-EFD GBV_NL_ARTICLE AR 106 2004 9 673-678 6 |
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10.1108/00070700410558201 doi (DE-627)NLEJ219608237 DE-627 ger DE-627 rakwb eng XA-GB Isolation and amplification of DNA from turmeric powder 2004 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier An efficient protocol for the isolation of high molecular weight DNA from dry powdered samples of turmeric including market samples is described which will help in PCR based detection of adulteration in marketed turmeric powders. The method involves a modified CTAB (3 per cent) procedure with 2?M NaCl, 0.3 per cent ß-mercaptoethanol coupled with purification of DNA in 30 per cent polyethylene glycol (8000). The yield of the DNA obtained from the samples varied from 2 to 4?µg/g tissue. The DNA obtained from the five different samples were consistently amplifiable (RAPD primers). Emerald Fulltext Archive Database 1994-2005 Food additives Food testing Remya, R. oth Syamkumar, S. oth Sasikumar, B. oth In British food journal Bradford : Emerald, 1899 106(2004), 9, Seite 673-678 Online-Ressource (DE-627)NLEJ219579555 (DE-600)2027461-0 nnns volume:106 year:2004 number:9 pages:673-678 extent:6 http://dx.doi.org/10.1108/00070700410558201 GBV_USEFLAG_U ZDB-1-EFD GBV_NL_ARTICLE AR 106 2004 9 673-678 6 |
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Isolation and amplification of DNA from turmeric powder |
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An efficient protocol for the isolation of high molecular weight DNA from dry powdered samples of turmeric including market samples is described which will help in PCR based detection of adulteration in marketed turmeric powders. The method involves a modified CTAB (3 per cent) procedure with 2?M NaCl, 0.3 per cent ß-mercaptoethanol coupled with purification of DNA in 30 per cent polyethylene glycol (8000). The yield of the DNA obtained from the samples varied from 2 to 4?µg/g tissue. The DNA obtained from the five different samples were consistently amplifiable (RAPD primers). |
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An efficient protocol for the isolation of high molecular weight DNA from dry powdered samples of turmeric including market samples is described which will help in PCR based detection of adulteration in marketed turmeric powders. The method involves a modified CTAB (3 per cent) procedure with 2?M NaCl, 0.3 per cent ß-mercaptoethanol coupled with purification of DNA in 30 per cent polyethylene glycol (8000). The yield of the DNA obtained from the samples varied from 2 to 4?µg/g tissue. The DNA obtained from the five different samples were consistently amplifiable (RAPD primers). |
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An efficient protocol for the isolation of high molecular weight DNA from dry powdered samples of turmeric including market samples is described which will help in PCR based detection of adulteration in marketed turmeric powders. The method involves a modified CTAB (3 per cent) procedure with 2?M NaCl, 0.3 per cent ß-mercaptoethanol coupled with purification of DNA in 30 per cent polyethylene glycol (8000). The yield of the DNA obtained from the samples varied from 2 to 4?µg/g tissue. The DNA obtained from the five different samples were consistently amplifiable (RAPD primers). |
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