A LOW AFFINITY, LOW MOLECULAR WEIGHT ENDOTHELIN-A RECEPTOR PRESENT IN NEONATAL RAT HEART
1. Addition of endothelin-1 (ET-1) to [3H]-inositol-labelled neonatal rat hearts stimulated the accumulation of [3H]-labelled inositol phosphates (InsP), but only at high concentrations; concentration at half maximum stimulation (EC50)>0.1 μmol/L). When similar experiments were performed using is...
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| Autor*in: |
Woodcock, Elizabeth A. [verfasserIn] Land, Sarah L. [verfasserIn] Andrews, Robert K. [verfasserIn] |
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| Format: |
E-Artikel |
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| Erschienen: |
Oxford, UK: Blackwell Publishing Ltd ; 1993 |
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| Schlagwörter: |
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| Umfang: |
Online-Ressource |
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| Reproduktion: |
2007 ; Blackwell Publishing Journal Backfiles 1879-2005 |
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| Übergeordnetes Werk: |
In: Clinical and experimental pharmacology and physiology - Oxford [u.a.] : Wiley-Blackwell, 1974, 20(1993), 5, Seite 0 |
| Übergeordnetes Werk: |
volume:20 ; year:1993 ; number:5 ; pages:0 |
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| DOI / URN: |
10.1111/j.1440-1681.1993.tb01697.x |
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| 520 | |a 1. Addition of endothelin-1 (ET-1) to [3H]-inositol-labelled neonatal rat hearts stimulated the accumulation of [3H]-labelled inositol phosphates (InsP), but only at high concentrations; concentration at half maximum stimulation (EC50)>0.1 μmol/L). When similar experiments were performed using isolated myocytes, the potency of endothelin-1 was higher and the EC50 value averaged 3.2 ± 0.5 nmol/L (mean±s.e.m., n = 4).2. The binding affinity of [125I]-endothelin-1 was higher for receptors on isolated cells than for receptors on membranes prepared from intact heart (72 ± 16 pmol/L compared with 3.9 ± 0.7 nmol/L, mean ± s.e.m., n= 4, P < 0.01; Students’t test).3. Receptors from both sources were cross-linked to [125I]-endothelin-1 and their molecular weights measured using sodium dodecylsulfate gradient polyacrylamide gel electrophoresis (SDS-PAGE). The receptors present on the isolated cells had a higher molecular weight (48 kD) than the receptor on the heart membranes (38 kD). | ||
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10.1111/j.1440-1681.1993.tb01697.x doi (DE-627)NLEJ238603016 DE-627 ger DE-627 rakwb Woodcock, Elizabeth A. verfasserin aut A LOW AFFINITY, LOW MOLECULAR WEIGHT ENDOTHELIN-A RECEPTOR PRESENT IN NEONATAL RAT HEART Oxford, UK Blackwell Publishing Ltd 1993 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier 1. Addition of endothelin-1 (ET-1) to [3H]-inositol-labelled neonatal rat hearts stimulated the accumulation of [3H]-labelled inositol phosphates (InsP), but only at high concentrations; concentration at half maximum stimulation (EC50)>0.1 μmol/L). When similar experiments were performed using isolated myocytes, the potency of endothelin-1 was higher and the EC50 value averaged 3.2 ± 0.5 nmol/L (mean±s.e.m., n = 4).2. The binding affinity of [125I]-endothelin-1 was higher for receptors on isolated cells than for receptors on membranes prepared from intact heart (72 ± 16 pmol/L compared with 3.9 ± 0.7 nmol/L, mean ± s.e.m., n= 4, P < 0.01; Students’t test).3. Receptors from both sources were cross-linked to [125I]-endothelin-1 and their molecular weights measured using sodium dodecylsulfate gradient polyacrylamide gel electrophoresis (SDS-PAGE). The receptors present on the isolated cells had a higher molecular weight (48 kD) than the receptor on the heart membranes (38 kD). 2007 Blackwell Publishing Journal Backfiles 1879-2005 |2007|||||||||| endothelin Land, Sarah L. verfasserin aut Andrews, Robert K. verfasserin aut In Clinical and experimental pharmacology and physiology Oxford [u.a.] : Wiley-Blackwell, 1974 20(1993), 5, Seite 0 Online-Ressource (DE-627)NLEJ243927150 (DE-600)2020033-X 1440-1681 nnns volume:20 year:1993 number:5 pages:0 http://dx.doi.org/10.1111/j.1440-1681.1993.tb01697.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 20 1993 5 0 |
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10.1111/j.1440-1681.1993.tb01697.x doi (DE-627)NLEJ238603016 DE-627 ger DE-627 rakwb Woodcock, Elizabeth A. verfasserin aut A LOW AFFINITY, LOW MOLECULAR WEIGHT ENDOTHELIN-A RECEPTOR PRESENT IN NEONATAL RAT HEART Oxford, UK Blackwell Publishing Ltd 1993 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier 1. Addition of endothelin-1 (ET-1) to [3H]-inositol-labelled neonatal rat hearts stimulated the accumulation of [3H]-labelled inositol phosphates (InsP), but only at high concentrations; concentration at half maximum stimulation (EC50)>0.1 μmol/L). When similar experiments were performed using isolated myocytes, the potency of endothelin-1 was higher and the EC50 value averaged 3.2 ± 0.5 nmol/L (mean±s.e.m., n = 4).2. The binding affinity of [125I]-endothelin-1 was higher for receptors on isolated cells than for receptors on membranes prepared from intact heart (72 ± 16 pmol/L compared with 3.9 ± 0.7 nmol/L, mean ± s.e.m., n= 4, P < 0.01; Students’t test).3. Receptors from both sources were cross-linked to [125I]-endothelin-1 and their molecular weights measured using sodium dodecylsulfate gradient polyacrylamide gel electrophoresis (SDS-PAGE). The receptors present on the isolated cells had a higher molecular weight (48 kD) than the receptor on the heart membranes (38 kD). 2007 Blackwell Publishing Journal Backfiles 1879-2005 |2007|||||||||| endothelin Land, Sarah L. verfasserin aut Andrews, Robert K. verfasserin aut In Clinical and experimental pharmacology and physiology Oxford [u.a.] : Wiley-Blackwell, 1974 20(1993), 5, Seite 0 Online-Ressource (DE-627)NLEJ243927150 (DE-600)2020033-X 1440-1681 nnns volume:20 year:1993 number:5 pages:0 http://dx.doi.org/10.1111/j.1440-1681.1993.tb01697.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 20 1993 5 0 |
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10.1111/j.1440-1681.1993.tb01697.x doi (DE-627)NLEJ238603016 DE-627 ger DE-627 rakwb Woodcock, Elizabeth A. verfasserin aut A LOW AFFINITY, LOW MOLECULAR WEIGHT ENDOTHELIN-A RECEPTOR PRESENT IN NEONATAL RAT HEART Oxford, UK Blackwell Publishing Ltd 1993 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier 1. Addition of endothelin-1 (ET-1) to [3H]-inositol-labelled neonatal rat hearts stimulated the accumulation of [3H]-labelled inositol phosphates (InsP), but only at high concentrations; concentration at half maximum stimulation (EC50)>0.1 μmol/L). When similar experiments were performed using isolated myocytes, the potency of endothelin-1 was higher and the EC50 value averaged 3.2 ± 0.5 nmol/L (mean±s.e.m., n = 4).2. The binding affinity of [125I]-endothelin-1 was higher for receptors on isolated cells than for receptors on membranes prepared from intact heart (72 ± 16 pmol/L compared with 3.9 ± 0.7 nmol/L, mean ± s.e.m., n= 4, P < 0.01; Students’t test).3. Receptors from both sources were cross-linked to [125I]-endothelin-1 and their molecular weights measured using sodium dodecylsulfate gradient polyacrylamide gel electrophoresis (SDS-PAGE). The receptors present on the isolated cells had a higher molecular weight (48 kD) than the receptor on the heart membranes (38 kD). 2007 Blackwell Publishing Journal Backfiles 1879-2005 |2007|||||||||| endothelin Land, Sarah L. verfasserin aut Andrews, Robert K. verfasserin aut In Clinical and experimental pharmacology and physiology Oxford [u.a.] : Wiley-Blackwell, 1974 20(1993), 5, Seite 0 Online-Ressource (DE-627)NLEJ243927150 (DE-600)2020033-X 1440-1681 nnns volume:20 year:1993 number:5 pages:0 http://dx.doi.org/10.1111/j.1440-1681.1993.tb01697.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 20 1993 5 0 |
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10.1111/j.1440-1681.1993.tb01697.x doi (DE-627)NLEJ238603016 DE-627 ger DE-627 rakwb Woodcock, Elizabeth A. verfasserin aut A LOW AFFINITY, LOW MOLECULAR WEIGHT ENDOTHELIN-A RECEPTOR PRESENT IN NEONATAL RAT HEART Oxford, UK Blackwell Publishing Ltd 1993 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier 1. Addition of endothelin-1 (ET-1) to [3H]-inositol-labelled neonatal rat hearts stimulated the accumulation of [3H]-labelled inositol phosphates (InsP), but only at high concentrations; concentration at half maximum stimulation (EC50)>0.1 μmol/L). When similar experiments were performed using isolated myocytes, the potency of endothelin-1 was higher and the EC50 value averaged 3.2 ± 0.5 nmol/L (mean±s.e.m., n = 4).2. The binding affinity of [125I]-endothelin-1 was higher for receptors on isolated cells than for receptors on membranes prepared from intact heart (72 ± 16 pmol/L compared with 3.9 ± 0.7 nmol/L, mean ± s.e.m., n= 4, P < 0.01; Students’t test).3. Receptors from both sources were cross-linked to [125I]-endothelin-1 and their molecular weights measured using sodium dodecylsulfate gradient polyacrylamide gel electrophoresis (SDS-PAGE). The receptors present on the isolated cells had a higher molecular weight (48 kD) than the receptor on the heart membranes (38 kD). 2007 Blackwell Publishing Journal Backfiles 1879-2005 |2007|||||||||| endothelin Land, Sarah L. verfasserin aut Andrews, Robert K. verfasserin aut In Clinical and experimental pharmacology and physiology Oxford [u.a.] : Wiley-Blackwell, 1974 20(1993), 5, Seite 0 Online-Ressource (DE-627)NLEJ243927150 (DE-600)2020033-X 1440-1681 nnns volume:20 year:1993 number:5 pages:0 http://dx.doi.org/10.1111/j.1440-1681.1993.tb01697.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 20 1993 5 0 |
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10.1111/j.1440-1681.1993.tb01697.x doi (DE-627)NLEJ238603016 DE-627 ger DE-627 rakwb Woodcock, Elizabeth A. verfasserin aut A LOW AFFINITY, LOW MOLECULAR WEIGHT ENDOTHELIN-A RECEPTOR PRESENT IN NEONATAL RAT HEART Oxford, UK Blackwell Publishing Ltd 1993 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier 1. Addition of endothelin-1 (ET-1) to [3H]-inositol-labelled neonatal rat hearts stimulated the accumulation of [3H]-labelled inositol phosphates (InsP), but only at high concentrations; concentration at half maximum stimulation (EC50)>0.1 μmol/L). When similar experiments were performed using isolated myocytes, the potency of endothelin-1 was higher and the EC50 value averaged 3.2 ± 0.5 nmol/L (mean±s.e.m., n = 4).2. The binding affinity of [125I]-endothelin-1 was higher for receptors on isolated cells than for receptors on membranes prepared from intact heart (72 ± 16 pmol/L compared with 3.9 ± 0.7 nmol/L, mean ± s.e.m., n= 4, P < 0.01; Students’t test).3. Receptors from both sources were cross-linked to [125I]-endothelin-1 and their molecular weights measured using sodium dodecylsulfate gradient polyacrylamide gel electrophoresis (SDS-PAGE). The receptors present on the isolated cells had a higher molecular weight (48 kD) than the receptor on the heart membranes (38 kD). 2007 Blackwell Publishing Journal Backfiles 1879-2005 |2007|||||||||| endothelin Land, Sarah L. verfasserin aut Andrews, Robert K. verfasserin aut In Clinical and experimental pharmacology and physiology Oxford [u.a.] : Wiley-Blackwell, 1974 20(1993), 5, Seite 0 Online-Ressource (DE-627)NLEJ243927150 (DE-600)2020033-X 1440-1681 nnns volume:20 year:1993 number:5 pages:0 http://dx.doi.org/10.1111/j.1440-1681.1993.tb01697.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 20 1993 5 0 |
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A LOW AFFINITY, LOW MOLECULAR WEIGHT ENDOTHELIN-A RECEPTOR PRESENT IN NEONATAL RAT HEART |
| abstract |
1. Addition of endothelin-1 (ET-1) to [3H]-inositol-labelled neonatal rat hearts stimulated the accumulation of [3H]-labelled inositol phosphates (InsP), but only at high concentrations; concentration at half maximum stimulation (EC50)>0.1 μmol/L). When similar experiments were performed using isolated myocytes, the potency of endothelin-1 was higher and the EC50 value averaged 3.2 ± 0.5 nmol/L (mean±s.e.m., n = 4).2. The binding affinity of [125I]-endothelin-1 was higher for receptors on isolated cells than for receptors on membranes prepared from intact heart (72 ± 16 pmol/L compared with 3.9 ± 0.7 nmol/L, mean ± s.e.m., n= 4, P < 0.01; Students’t test).3. Receptors from both sources were cross-linked to [125I]-endothelin-1 and their molecular weights measured using sodium dodecylsulfate gradient polyacrylamide gel electrophoresis (SDS-PAGE). The receptors present on the isolated cells had a higher molecular weight (48 kD) than the receptor on the heart membranes (38 kD). |
| abstractGer |
1. Addition of endothelin-1 (ET-1) to [3H]-inositol-labelled neonatal rat hearts stimulated the accumulation of [3H]-labelled inositol phosphates (InsP), but only at high concentrations; concentration at half maximum stimulation (EC50)>0.1 μmol/L). When similar experiments were performed using isolated myocytes, the potency of endothelin-1 was higher and the EC50 value averaged 3.2 ± 0.5 nmol/L (mean±s.e.m., n = 4).2. The binding affinity of [125I]-endothelin-1 was higher for receptors on isolated cells than for receptors on membranes prepared from intact heart (72 ± 16 pmol/L compared with 3.9 ± 0.7 nmol/L, mean ± s.e.m., n= 4, P < 0.01; Students’t test).3. Receptors from both sources were cross-linked to [125I]-endothelin-1 and their molecular weights measured using sodium dodecylsulfate gradient polyacrylamide gel electrophoresis (SDS-PAGE). The receptors present on the isolated cells had a higher molecular weight (48 kD) than the receptor on the heart membranes (38 kD). |
| abstract_unstemmed |
1. Addition of endothelin-1 (ET-1) to [3H]-inositol-labelled neonatal rat hearts stimulated the accumulation of [3H]-labelled inositol phosphates (InsP), but only at high concentrations; concentration at half maximum stimulation (EC50)>0.1 μmol/L). When similar experiments were performed using isolated myocytes, the potency of endothelin-1 was higher and the EC50 value averaged 3.2 ± 0.5 nmol/L (mean±s.e.m., n = 4).2. The binding affinity of [125I]-endothelin-1 was higher for receptors on isolated cells than for receptors on membranes prepared from intact heart (72 ± 16 pmol/L compared with 3.9 ± 0.7 nmol/L, mean ± s.e.m., n= 4, P < 0.01; Students’t test).3. Receptors from both sources were cross-linked to [125I]-endothelin-1 and their molecular weights measured using sodium dodecylsulfate gradient polyacrylamide gel electrophoresis (SDS-PAGE). The receptors present on the isolated cells had a higher molecular weight (48 kD) than the receptor on the heart membranes (38 kD). |
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A LOW AFFINITY, LOW MOLECULAR WEIGHT ENDOTHELIN-A RECEPTOR PRESENT IN NEONATAL RAT HEART |
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Land, Sarah L. Andrews, Robert K. |
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10.1111/j.1440-1681.1993.tb01697.x |
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