A Three-Layer Immunoradiometric Assay for Determination of IgG Subclass Antibodies in Human Sera (“IgG Subclass RAST”)
We report the development of a three-layer immunoradiometric assay (TIRA) for measurement of IgG antibodies of all four subclasses in human sera. The first layer consists of diluted human serum, the second layer is monoclonal mouse antibodies to human IgG subclasses., and the third layer is 125I-lab...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Djurup, R. [verfasserIn] Søndergaard, I [verfasserIn] Magnusson, C. G. M. [verfasserIn] |
|---|
| Format: |
E-Artikel |
|---|
| Erschienen: |
Oxford, UK: Blackwell Publishing Ltd ; 1984 |
|---|
| Schlagwörter: |
|---|
| Umfang: |
Online-Ressource |
|---|
| Reproduktion: |
2007 ; Blackwell Publishing Journal Backfiles 1879-2005 |
|---|---|
| Übergeordnetes Werk: |
In: Allergy - Oxford : Blackwell Munksgaard, 1978, 39(1984), 1, Seite 0 |
| Übergeordnetes Werk: |
volume:39 ; year:1984 ; number:1 ; pages:0 |
| Links: |
|---|
| DOI / URN: |
10.1111/j.1398-9995.1984.tb01933.x |
|---|
| Katalog-ID: |
NLEJ239294955 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLEJ239294955 | ||
| 003 | DE-627 | ||
| 005 | 20210707082937.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 120426s1984 xx |||||o 00| ||und c | ||
| 024 | 7 | |a 10.1111/j.1398-9995.1984.tb01933.x |2 doi | |
| 035 | |a (DE-627)NLEJ239294955 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 100 | 1 | |a Djurup, R. |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a A Three-Layer Immunoradiometric Assay for Determination of IgG Subclass Antibodies in Human Sera (“IgG Subclass RAST”) |
| 264 | 1 | |a Oxford, UK |b Blackwell Publishing Ltd |c 1984 | |
| 300 | |a Online-Ressource | ||
| 336 | |a nicht spezifiziert |b zzz |2 rdacontent | ||
| 337 | |a nicht spezifiziert |b z |2 rdamedia | ||
| 338 | |a nicht spezifiziert |b zu |2 rdacarrier | ||
| 520 | |a We report the development of a three-layer immunoradiometric assay (TIRA) for measurement of IgG antibodies of all four subclasses in human sera. The first layer consists of diluted human serum, the second layer is monoclonal mouse antibodies to human IgG subclasses., and the third layer is 125I-labelled rabbit anti-mouse IgG. Monoclonal anti-IgG1. anti-IgC3 and anti-IgG4 reacted only with their complementary IgG subclass, whereas the anti-lgG2 showed slight cross-reactivity to immunoglobins of other subclasses and classes and to light chain proteins. The observed cross-reactivity was found to be without importance, when the TIRA was applied to measurement of IgG subclass antibodies. Equipotency was established by use of appropriate dilutions of the monoclonal antibodies, and the assay was calibrated by use of human reference serum. The TIRA therefore permits reliable inter-individual and intra-individual comparisons of the IgG antibody response in all four subclasses. Von-sped fie binding obtained with pooled normal human serum was below 0.33′#. Inter-assay coefficient of variation was between 18 and 27%, The TIRA was applied to measurement of IgG subclass antibodies to timothy grass pollen in sera from grass pollen allergies undergoing immunotherapy. | ||
| 533 | |d 2007 |f Blackwell Publishing Journal Backfiles 1879-2005 |7 |2007|||||||||| | ||
| 650 | 4 | |a blocking antibodies | |
| 700 | 1 | |a Søndergaard, I |e verfasserin |4 aut | |
| 700 | 1 | |a Magnusson, C. G. M. |e verfasserin |4 aut | |
| 700 | 1 | |a Weeke, B. |4 oth | |
| 773 | 0 | 8 | |i In |t Allergy |d Oxford : Blackwell Munksgaard, 1978 |g 39(1984), 1, Seite 0 |h Online-Ressource |w (DE-627)NLEJ243926979 |w (DE-600)2003114-2 |x 1398-9995 |7 nnns |
| 773 | 1 | 8 | |g volume:39 |g year:1984 |g number:1 |g pages:0 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1111/j.1398-9995.1984.tb01933.x |q text/html |x Verlag |z Deutschlandweit zugänglich |3 Volltext |
| 912 | |a GBV_USEFLAG_U | ||
| 912 | |a ZDB-1-DJB | ||
| 912 | |a GBV_NL_ARTICLE | ||
| 951 | |a AR | ||
| 952 | |d 39 |j 1984 |e 1 |h 0 | ||
| author_variant |
r d rd i s is c g m m cgm cgmm |
|---|---|
| matchkey_str |
article:13989995:1984----::treaeimnrdoercsafreemntooigucasniois |
| hierarchy_sort_str |
1984 |
| publishDate |
1984 |
| allfields |
10.1111/j.1398-9995.1984.tb01933.x doi (DE-627)NLEJ239294955 DE-627 ger DE-627 rakwb Djurup, R. verfasserin aut A Three-Layer Immunoradiometric Assay for Determination of IgG Subclass Antibodies in Human Sera (“IgG Subclass RAST”) Oxford, UK Blackwell Publishing Ltd 1984 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier We report the development of a three-layer immunoradiometric assay (TIRA) for measurement of IgG antibodies of all four subclasses in human sera. The first layer consists of diluted human serum, the second layer is monoclonal mouse antibodies to human IgG subclasses., and the third layer is 125I-labelled rabbit anti-mouse IgG. Monoclonal anti-IgG1. anti-IgC3 and anti-IgG4 reacted only with their complementary IgG subclass, whereas the anti-lgG2 showed slight cross-reactivity to immunoglobins of other subclasses and classes and to light chain proteins. The observed cross-reactivity was found to be without importance, when the TIRA was applied to measurement of IgG subclass antibodies. Equipotency was established by use of appropriate dilutions of the monoclonal antibodies, and the assay was calibrated by use of human reference serum. The TIRA therefore permits reliable inter-individual and intra-individual comparisons of the IgG antibody response in all four subclasses. Von-sped fie binding obtained with pooled normal human serum was below 0.33′#. Inter-assay coefficient of variation was between 18 and 27%, The TIRA was applied to measurement of IgG subclass antibodies to timothy grass pollen in sera from grass pollen allergies undergoing immunotherapy. 2007 Blackwell Publishing Journal Backfiles 1879-2005 |2007|||||||||| blocking antibodies Søndergaard, I verfasserin aut Magnusson, C. G. M. verfasserin aut Weeke, B. oth In Allergy Oxford : Blackwell Munksgaard, 1978 39(1984), 1, Seite 0 Online-Ressource (DE-627)NLEJ243926979 (DE-600)2003114-2 1398-9995 nnns volume:39 year:1984 number:1 pages:0 http://dx.doi.org/10.1111/j.1398-9995.1984.tb01933.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 39 1984 1 0 |
| spelling |
10.1111/j.1398-9995.1984.tb01933.x doi (DE-627)NLEJ239294955 DE-627 ger DE-627 rakwb Djurup, R. verfasserin aut A Three-Layer Immunoradiometric Assay for Determination of IgG Subclass Antibodies in Human Sera (“IgG Subclass RAST”) Oxford, UK Blackwell Publishing Ltd 1984 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier We report the development of a three-layer immunoradiometric assay (TIRA) for measurement of IgG antibodies of all four subclasses in human sera. The first layer consists of diluted human serum, the second layer is monoclonal mouse antibodies to human IgG subclasses., and the third layer is 125I-labelled rabbit anti-mouse IgG. Monoclonal anti-IgG1. anti-IgC3 and anti-IgG4 reacted only with their complementary IgG subclass, whereas the anti-lgG2 showed slight cross-reactivity to immunoglobins of other subclasses and classes and to light chain proteins. The observed cross-reactivity was found to be without importance, when the TIRA was applied to measurement of IgG subclass antibodies. Equipotency was established by use of appropriate dilutions of the monoclonal antibodies, and the assay was calibrated by use of human reference serum. The TIRA therefore permits reliable inter-individual and intra-individual comparisons of the IgG antibody response in all four subclasses. Von-sped fie binding obtained with pooled normal human serum was below 0.33′#. Inter-assay coefficient of variation was between 18 and 27%, The TIRA was applied to measurement of IgG subclass antibodies to timothy grass pollen in sera from grass pollen allergies undergoing immunotherapy. 2007 Blackwell Publishing Journal Backfiles 1879-2005 |2007|||||||||| blocking antibodies Søndergaard, I verfasserin aut Magnusson, C. G. M. verfasserin aut Weeke, B. oth In Allergy Oxford : Blackwell Munksgaard, 1978 39(1984), 1, Seite 0 Online-Ressource (DE-627)NLEJ243926979 (DE-600)2003114-2 1398-9995 nnns volume:39 year:1984 number:1 pages:0 http://dx.doi.org/10.1111/j.1398-9995.1984.tb01933.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 39 1984 1 0 |
| allfields_unstemmed |
10.1111/j.1398-9995.1984.tb01933.x doi (DE-627)NLEJ239294955 DE-627 ger DE-627 rakwb Djurup, R. verfasserin aut A Three-Layer Immunoradiometric Assay for Determination of IgG Subclass Antibodies in Human Sera (“IgG Subclass RAST”) Oxford, UK Blackwell Publishing Ltd 1984 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier We report the development of a three-layer immunoradiometric assay (TIRA) for measurement of IgG antibodies of all four subclasses in human sera. The first layer consists of diluted human serum, the second layer is monoclonal mouse antibodies to human IgG subclasses., and the third layer is 125I-labelled rabbit anti-mouse IgG. Monoclonal anti-IgG1. anti-IgC3 and anti-IgG4 reacted only with their complementary IgG subclass, whereas the anti-lgG2 showed slight cross-reactivity to immunoglobins of other subclasses and classes and to light chain proteins. The observed cross-reactivity was found to be without importance, when the TIRA was applied to measurement of IgG subclass antibodies. Equipotency was established by use of appropriate dilutions of the monoclonal antibodies, and the assay was calibrated by use of human reference serum. The TIRA therefore permits reliable inter-individual and intra-individual comparisons of the IgG antibody response in all four subclasses. Von-sped fie binding obtained with pooled normal human serum was below 0.33′#. Inter-assay coefficient of variation was between 18 and 27%, The TIRA was applied to measurement of IgG subclass antibodies to timothy grass pollen in sera from grass pollen allergies undergoing immunotherapy. 2007 Blackwell Publishing Journal Backfiles 1879-2005 |2007|||||||||| blocking antibodies Søndergaard, I verfasserin aut Magnusson, C. G. M. verfasserin aut Weeke, B. oth In Allergy Oxford : Blackwell Munksgaard, 1978 39(1984), 1, Seite 0 Online-Ressource (DE-627)NLEJ243926979 (DE-600)2003114-2 1398-9995 nnns volume:39 year:1984 number:1 pages:0 http://dx.doi.org/10.1111/j.1398-9995.1984.tb01933.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 39 1984 1 0 |
| allfieldsGer |
10.1111/j.1398-9995.1984.tb01933.x doi (DE-627)NLEJ239294955 DE-627 ger DE-627 rakwb Djurup, R. verfasserin aut A Three-Layer Immunoradiometric Assay for Determination of IgG Subclass Antibodies in Human Sera (“IgG Subclass RAST”) Oxford, UK Blackwell Publishing Ltd 1984 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier We report the development of a three-layer immunoradiometric assay (TIRA) for measurement of IgG antibodies of all four subclasses in human sera. The first layer consists of diluted human serum, the second layer is monoclonal mouse antibodies to human IgG subclasses., and the third layer is 125I-labelled rabbit anti-mouse IgG. Monoclonal anti-IgG1. anti-IgC3 and anti-IgG4 reacted only with their complementary IgG subclass, whereas the anti-lgG2 showed slight cross-reactivity to immunoglobins of other subclasses and classes and to light chain proteins. The observed cross-reactivity was found to be without importance, when the TIRA was applied to measurement of IgG subclass antibodies. Equipotency was established by use of appropriate dilutions of the monoclonal antibodies, and the assay was calibrated by use of human reference serum. The TIRA therefore permits reliable inter-individual and intra-individual comparisons of the IgG antibody response in all four subclasses. Von-sped fie binding obtained with pooled normal human serum was below 0.33′#. Inter-assay coefficient of variation was between 18 and 27%, The TIRA was applied to measurement of IgG subclass antibodies to timothy grass pollen in sera from grass pollen allergies undergoing immunotherapy. 2007 Blackwell Publishing Journal Backfiles 1879-2005 |2007|||||||||| blocking antibodies Søndergaard, I verfasserin aut Magnusson, C. G. M. verfasserin aut Weeke, B. oth In Allergy Oxford : Blackwell Munksgaard, 1978 39(1984), 1, Seite 0 Online-Ressource (DE-627)NLEJ243926979 (DE-600)2003114-2 1398-9995 nnns volume:39 year:1984 number:1 pages:0 http://dx.doi.org/10.1111/j.1398-9995.1984.tb01933.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 39 1984 1 0 |
| allfieldsSound |
10.1111/j.1398-9995.1984.tb01933.x doi (DE-627)NLEJ239294955 DE-627 ger DE-627 rakwb Djurup, R. verfasserin aut A Three-Layer Immunoradiometric Assay for Determination of IgG Subclass Antibodies in Human Sera (“IgG Subclass RAST”) Oxford, UK Blackwell Publishing Ltd 1984 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier We report the development of a three-layer immunoradiometric assay (TIRA) for measurement of IgG antibodies of all four subclasses in human sera. The first layer consists of diluted human serum, the second layer is monoclonal mouse antibodies to human IgG subclasses., and the third layer is 125I-labelled rabbit anti-mouse IgG. Monoclonal anti-IgG1. anti-IgC3 and anti-IgG4 reacted only with their complementary IgG subclass, whereas the anti-lgG2 showed slight cross-reactivity to immunoglobins of other subclasses and classes and to light chain proteins. The observed cross-reactivity was found to be without importance, when the TIRA was applied to measurement of IgG subclass antibodies. Equipotency was established by use of appropriate dilutions of the monoclonal antibodies, and the assay was calibrated by use of human reference serum. The TIRA therefore permits reliable inter-individual and intra-individual comparisons of the IgG antibody response in all four subclasses. Von-sped fie binding obtained with pooled normal human serum was below 0.33′#. Inter-assay coefficient of variation was between 18 and 27%, The TIRA was applied to measurement of IgG subclass antibodies to timothy grass pollen in sera from grass pollen allergies undergoing immunotherapy. 2007 Blackwell Publishing Journal Backfiles 1879-2005 |2007|||||||||| blocking antibodies Søndergaard, I verfasserin aut Magnusson, C. G. M. verfasserin aut Weeke, B. oth In Allergy Oxford : Blackwell Munksgaard, 1978 39(1984), 1, Seite 0 Online-Ressource (DE-627)NLEJ243926979 (DE-600)2003114-2 1398-9995 nnns volume:39 year:1984 number:1 pages:0 http://dx.doi.org/10.1111/j.1398-9995.1984.tb01933.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 39 1984 1 0 |
| source |
In Allergy 39(1984), 1, Seite 0 volume:39 year:1984 number:1 pages:0 |
| sourceStr |
In Allergy 39(1984), 1, Seite 0 volume:39 year:1984 number:1 pages:0 |
| format_phy_str_mv |
Article |
| institution |
findex.gbv.de |
| topic_facet |
blocking antibodies |
| isfreeaccess_bool |
false |
| container_title |
Allergy |
| authorswithroles_txt_mv |
Djurup, R. @@aut@@ Søndergaard, I @@aut@@ Magnusson, C. G. M. @@aut@@ Weeke, B. @@oth@@ |
| publishDateDaySort_date |
1984-01-01T00:00:00Z |
| hierarchy_top_id |
NLEJ243926979 |
| id |
NLEJ239294955 |
| fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ239294955</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210707082937.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">120426s1984 xx |||||o 00| ||und c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1111/j.1398-9995.1984.tb01933.x</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ239294955</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Djurup, R.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">A Three-Layer Immunoradiometric Assay for Determination of IgG Subclass Antibodies in Human Sera (“IgG Subclass RAST”)</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Oxford, UK</subfield><subfield code="b">Blackwell Publishing Ltd</subfield><subfield code="c">1984</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">We report the development of a three-layer immunoradiometric assay (TIRA) for measurement of IgG antibodies of all four subclasses in human sera. The first layer consists of diluted human serum, the second layer is monoclonal mouse antibodies to human IgG subclasses., and the third layer is 125I-labelled rabbit anti-mouse IgG. Monoclonal anti-IgG1. anti-IgC3 and anti-IgG4 reacted only with their complementary IgG subclass, whereas the anti-lgG2 showed slight cross-reactivity to immunoglobins of other subclasses and classes and to light chain proteins. The observed cross-reactivity was found to be without importance, when the TIRA was applied to measurement of IgG subclass antibodies. Equipotency was established by use of appropriate dilutions of the monoclonal antibodies, and the assay was calibrated by use of human reference serum. The TIRA therefore permits reliable inter-individual and intra-individual comparisons of the IgG antibody response in all four subclasses. Von-sped fie binding obtained with pooled normal human serum was below 0.33′#. Inter-assay coefficient of variation was between 18 and 27%, The TIRA was applied to measurement of IgG subclass antibodies to timothy grass pollen in sera from grass pollen allergies undergoing immunotherapy.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="d">2007</subfield><subfield code="f">Blackwell Publishing Journal Backfiles 1879-2005</subfield><subfield code="7">|2007||||||||||</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">blocking antibodies</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Søndergaard, I</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Magnusson, C. G. M.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Weeke, B.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Allergy</subfield><subfield code="d">Oxford : Blackwell Munksgaard, 1978</subfield><subfield code="g">39(1984), 1, Seite 0</subfield><subfield code="h">Online-Ressource</subfield><subfield code="w">(DE-627)NLEJ243926979</subfield><subfield code="w">(DE-600)2003114-2</subfield><subfield code="x">1398-9995</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:39</subfield><subfield code="g">year:1984</subfield><subfield code="g">number:1</subfield><subfield code="g">pages:0</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1111/j.1398-9995.1984.tb01933.x</subfield><subfield code="q">text/html</subfield><subfield code="x">Verlag</subfield><subfield code="z">Deutschlandweit zugänglich</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-DJB</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">39</subfield><subfield code="j">1984</subfield><subfield code="e">1</subfield><subfield code="h">0</subfield></datafield></record></collection>
|
| series2 |
Blackwell Publishing Journal Backfiles 1879-2005 |
| author |
Djurup, R. |
| spellingShingle |
Djurup, R. misc blocking antibodies A Three-Layer Immunoradiometric Assay for Determination of IgG Subclass Antibodies in Human Sera (“IgG Subclass RAST”) |
| authorStr |
Djurup, R. |
| ppnlink_with_tag_str_mv |
@@773@@(DE-627)NLEJ243926979 |
| format |
electronic Article |
| delete_txt_mv |
keep |
| author_role |
aut aut aut |
| collection |
NL |
| publishPlace |
Oxford, UK |
| remote_str |
true |
| illustrated |
Not Illustrated |
| issn |
1398-9995 |
| topic_title |
A Three-Layer Immunoradiometric Assay for Determination of IgG Subclass Antibodies in Human Sera (“IgG Subclass RAST”) blocking antibodies |
| publisher |
Blackwell Publishing Ltd |
| publisherStr |
Blackwell Publishing Ltd |
| topic |
misc blocking antibodies |
| topic_unstemmed |
misc blocking antibodies |
| topic_browse |
misc blocking antibodies |
| format_facet |
Elektronische Aufsätze Aufsätze Elektronische Ressource |
| format_main_str_mv |
Text Zeitschrift/Artikel |
| carriertype_str_mv |
zu |
| author2_variant |
b w bw |
| hierarchy_parent_title |
Allergy |
| hierarchy_parent_id |
NLEJ243926979 |
| hierarchy_top_title |
Allergy |
| isfreeaccess_txt |
false |
| familylinks_str_mv |
(DE-627)NLEJ243926979 (DE-600)2003114-2 |
| title |
A Three-Layer Immunoradiometric Assay for Determination of IgG Subclass Antibodies in Human Sera (“IgG Subclass RAST”) |
| ctrlnum |
(DE-627)NLEJ239294955 |
| title_full |
A Three-Layer Immunoradiometric Assay for Determination of IgG Subclass Antibodies in Human Sera (“IgG Subclass RAST”) |
| author_sort |
Djurup, R. |
| journal |
Allergy |
| journalStr |
Allergy |
| isOA_bool |
false |
| recordtype |
marc |
| publishDateSort |
1984 |
| contenttype_str_mv |
zzz |
| container_start_page |
0 |
| author_browse |
Djurup, R. Søndergaard, I Magnusson, C. G. M. |
| container_volume |
39 |
| physical |
Online-Ressource |
| format_se |
Elektronische Aufsätze |
| author-letter |
Djurup, R. |
| doi_str_mv |
10.1111/j.1398-9995.1984.tb01933.x |
| author2-role |
verfasserin |
| title_sort |
a three-layer immunoradiometric assay for determination of igg subclass antibodies in human sera (“igg subclass rast”) |
| title_auth |
A Three-Layer Immunoradiometric Assay for Determination of IgG Subclass Antibodies in Human Sera (“IgG Subclass RAST”) |
| abstract |
We report the development of a three-layer immunoradiometric assay (TIRA) for measurement of IgG antibodies of all four subclasses in human sera. The first layer consists of diluted human serum, the second layer is monoclonal mouse antibodies to human IgG subclasses., and the third layer is 125I-labelled rabbit anti-mouse IgG. Monoclonal anti-IgG1. anti-IgC3 and anti-IgG4 reacted only with their complementary IgG subclass, whereas the anti-lgG2 showed slight cross-reactivity to immunoglobins of other subclasses and classes and to light chain proteins. The observed cross-reactivity was found to be without importance, when the TIRA was applied to measurement of IgG subclass antibodies. Equipotency was established by use of appropriate dilutions of the monoclonal antibodies, and the assay was calibrated by use of human reference serum. The TIRA therefore permits reliable inter-individual and intra-individual comparisons of the IgG antibody response in all four subclasses. Von-sped fie binding obtained with pooled normal human serum was below 0.33′#. Inter-assay coefficient of variation was between 18 and 27%, The TIRA was applied to measurement of IgG subclass antibodies to timothy grass pollen in sera from grass pollen allergies undergoing immunotherapy. |
| abstractGer |
We report the development of a three-layer immunoradiometric assay (TIRA) for measurement of IgG antibodies of all four subclasses in human sera. The first layer consists of diluted human serum, the second layer is monoclonal mouse antibodies to human IgG subclasses., and the third layer is 125I-labelled rabbit anti-mouse IgG. Monoclonal anti-IgG1. anti-IgC3 and anti-IgG4 reacted only with their complementary IgG subclass, whereas the anti-lgG2 showed slight cross-reactivity to immunoglobins of other subclasses and classes and to light chain proteins. The observed cross-reactivity was found to be without importance, when the TIRA was applied to measurement of IgG subclass antibodies. Equipotency was established by use of appropriate dilutions of the monoclonal antibodies, and the assay was calibrated by use of human reference serum. The TIRA therefore permits reliable inter-individual and intra-individual comparisons of the IgG antibody response in all four subclasses. Von-sped fie binding obtained with pooled normal human serum was below 0.33′#. Inter-assay coefficient of variation was between 18 and 27%, The TIRA was applied to measurement of IgG subclass antibodies to timothy grass pollen in sera from grass pollen allergies undergoing immunotherapy. |
| abstract_unstemmed |
We report the development of a three-layer immunoradiometric assay (TIRA) for measurement of IgG antibodies of all four subclasses in human sera. The first layer consists of diluted human serum, the second layer is monoclonal mouse antibodies to human IgG subclasses., and the third layer is 125I-labelled rabbit anti-mouse IgG. Monoclonal anti-IgG1. anti-IgC3 and anti-IgG4 reacted only with their complementary IgG subclass, whereas the anti-lgG2 showed slight cross-reactivity to immunoglobins of other subclasses and classes and to light chain proteins. The observed cross-reactivity was found to be without importance, when the TIRA was applied to measurement of IgG subclass antibodies. Equipotency was established by use of appropriate dilutions of the monoclonal antibodies, and the assay was calibrated by use of human reference serum. The TIRA therefore permits reliable inter-individual and intra-individual comparisons of the IgG antibody response in all four subclasses. Von-sped fie binding obtained with pooled normal human serum was below 0.33′#. Inter-assay coefficient of variation was between 18 and 27%, The TIRA was applied to measurement of IgG subclass antibodies to timothy grass pollen in sera from grass pollen allergies undergoing immunotherapy. |
| collection_details |
GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE |
| container_issue |
1 |
| title_short |
A Three-Layer Immunoradiometric Assay for Determination of IgG Subclass Antibodies in Human Sera (“IgG Subclass RAST”) |
| url |
http://dx.doi.org/10.1111/j.1398-9995.1984.tb01933.x |
| remote_bool |
true |
| author2 |
Søndergaard, I Magnusson, C. G. M. Weeke, B. |
| author2Str |
Søndergaard, I Magnusson, C. G. M. Weeke, B. |
| ppnlink |
NLEJ243926979 |
| mediatype_str_mv |
z |
| isOA_txt |
false |
| hochschulschrift_bool |
false |
| author2_role |
oth |
| doi_str |
10.1111/j.1398-9995.1984.tb01933.x |
| up_date |
2024-07-06T07:08:35.971Z |
| _version_ |
1803812566175055872 |
| fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ239294955</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210707082937.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">120426s1984 xx |||||o 00| ||und c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1111/j.1398-9995.1984.tb01933.x</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ239294955</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Djurup, R.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">A Three-Layer Immunoradiometric Assay for Determination of IgG Subclass Antibodies in Human Sera (“IgG Subclass RAST”)</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Oxford, UK</subfield><subfield code="b">Blackwell Publishing Ltd</subfield><subfield code="c">1984</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">We report the development of a three-layer immunoradiometric assay (TIRA) for measurement of IgG antibodies of all four subclasses in human sera. The first layer consists of diluted human serum, the second layer is monoclonal mouse antibodies to human IgG subclasses., and the third layer is 125I-labelled rabbit anti-mouse IgG. Monoclonal anti-IgG1. anti-IgC3 and anti-IgG4 reacted only with their complementary IgG subclass, whereas the anti-lgG2 showed slight cross-reactivity to immunoglobins of other subclasses and classes and to light chain proteins. The observed cross-reactivity was found to be without importance, when the TIRA was applied to measurement of IgG subclass antibodies. Equipotency was established by use of appropriate dilutions of the monoclonal antibodies, and the assay was calibrated by use of human reference serum. The TIRA therefore permits reliable inter-individual and intra-individual comparisons of the IgG antibody response in all four subclasses. Von-sped fie binding obtained with pooled normal human serum was below 0.33′#. Inter-assay coefficient of variation was between 18 and 27%, The TIRA was applied to measurement of IgG subclass antibodies to timothy grass pollen in sera from grass pollen allergies undergoing immunotherapy.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="d">2007</subfield><subfield code="f">Blackwell Publishing Journal Backfiles 1879-2005</subfield><subfield code="7">|2007||||||||||</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">blocking antibodies</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Søndergaard, I</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Magnusson, C. G. M.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Weeke, B.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Allergy</subfield><subfield code="d">Oxford : Blackwell Munksgaard, 1978</subfield><subfield code="g">39(1984), 1, Seite 0</subfield><subfield code="h">Online-Ressource</subfield><subfield code="w">(DE-627)NLEJ243926979</subfield><subfield code="w">(DE-600)2003114-2</subfield><subfield code="x">1398-9995</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:39</subfield><subfield code="g">year:1984</subfield><subfield code="g">number:1</subfield><subfield code="g">pages:0</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1111/j.1398-9995.1984.tb01933.x</subfield><subfield code="q">text/html</subfield><subfield code="x">Verlag</subfield><subfield code="z">Deutschlandweit zugänglich</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-DJB</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">39</subfield><subfield code="j">1984</subfield><subfield code="e">1</subfield><subfield code="h">0</subfield></datafield></record></collection>
|
| score |
7.401019 |