The utilisation of arginine by oral streptococci grown glucose-limited in a chemostat
Abstract Pure cultures of 2 strains of Streptococcus milleri and one of Streptococcus mutans were grown glucose-limited at dilution rates of D= 0.1 h−1 and D= 0.3 h−1 and controlled pH of 7.0 in a chemically defined medium containing arginine levels of 0.6, 10, 33, 60 and 74 mM. Culture filtrate lev...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Rogers, A.H. [verfasserIn] Zilm, P.S. [verfasserIn] Gully, N.J. [verfasserIn] |
|---|
| Format: |
E-Artikel |
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| Erschienen: |
Oxford, UK: Blackwell Publishing Ltd ; 1986 |
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| Umfang: |
Online-Ressource |
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| Reproduktion: |
2006 ; Blackwell Publishing Journal Backfiles 1879-2005 |
|---|---|
| Übergeordnetes Werk: |
In: FEMS microbiology letters - Federation of European Microbiological Societies ; GKD-ID: 114439X, Oxford [u.a.] : Wiley-Blackwell, 1977, 37(1986), 1, Seite 0 |
| Übergeordnetes Werk: |
volume:37 ; year:1986 ; number:1 ; pages:0 |
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| DOI / URN: |
10.1111/j.1574-6968.1986.tb01757.x |
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NLEJ239760166 |
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| 520 | |a Abstract Pure cultures of 2 strains of Streptococcus milleri and one of Streptococcus mutans were grown glucose-limited at dilution rates of D= 0.1 h−1 and D= 0.3 h−1 and controlled pH of 7.0 in a chemically defined medium containing arginine levels of 0.6, 10, 33, 60 and 74 mM. Culture filtrate levels of arginine and its metabolites and glucose were determined as were cell dry weights and intracellular glycogen (IG).In S. milleri, stoichiometric conversion of up to 60 mM of arginine to citrulline and ornithine occurred, with 75% of the arginine appearing as ornithine. Similar patterns were obtained at D= 0.3 h−1, although only 30–40% of the arginine was converted to ornithine. At the 10 mM arginine level, biomass but no IG was produced while above this level, IG was also synthesised. At D= 0.3 h−1, substantial amounts of IG were detected at all arginine levels. As expected, S. mutans did not respond to increasing arginine levels and the use of this amino acid as an additional carbon and energy source with the release of ammonia in organisms such as S. milleri and Streptococcus sanguis may help to explain their dominance when growing in vitro and in vivo with S. mutans under glucose-limiting conditions. | ||
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10.1111/j.1574-6968.1986.tb01757.x doi (DE-627)NLEJ239760166 DE-627 ger DE-627 rakwb Rogers, A.H. verfasserin aut The utilisation of arginine by oral streptococci grown glucose-limited in a chemostat Oxford, UK Blackwell Publishing Ltd 1986 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract Pure cultures of 2 strains of Streptococcus milleri and one of Streptococcus mutans were grown glucose-limited at dilution rates of D= 0.1 h−1 and D= 0.3 h−1 and controlled pH of 7.0 in a chemically defined medium containing arginine levels of 0.6, 10, 33, 60 and 74 mM. Culture filtrate levels of arginine and its metabolites and glucose were determined as were cell dry weights and intracellular glycogen (IG).In S. milleri, stoichiometric conversion of up to 60 mM of arginine to citrulline and ornithine occurred, with 75% of the arginine appearing as ornithine. Similar patterns were obtained at D= 0.3 h−1, although only 30–40% of the arginine was converted to ornithine. At the 10 mM arginine level, biomass but no IG was produced while above this level, IG was also synthesised. At D= 0.3 h−1, substantial amounts of IG were detected at all arginine levels. As expected, S. mutans did not respond to increasing arginine levels and the use of this amino acid as an additional carbon and energy source with the release of ammonia in organisms such as S. milleri and Streptococcus sanguis may help to explain their dominance when growing in vitro and in vivo with S. mutans under glucose-limiting conditions. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Zilm, P.S. verfasserin aut Gully, N.J. verfasserin aut In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 37(1986), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:37 year:1986 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6968.1986.tb01757.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 37 1986 1 0 |
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10.1111/j.1574-6968.1986.tb01757.x doi (DE-627)NLEJ239760166 DE-627 ger DE-627 rakwb Rogers, A.H. verfasserin aut The utilisation of arginine by oral streptococci grown glucose-limited in a chemostat Oxford, UK Blackwell Publishing Ltd 1986 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract Pure cultures of 2 strains of Streptococcus milleri and one of Streptococcus mutans were grown glucose-limited at dilution rates of D= 0.1 h−1 and D= 0.3 h−1 and controlled pH of 7.0 in a chemically defined medium containing arginine levels of 0.6, 10, 33, 60 and 74 mM. Culture filtrate levels of arginine and its metabolites and glucose were determined as were cell dry weights and intracellular glycogen (IG).In S. milleri, stoichiometric conversion of up to 60 mM of arginine to citrulline and ornithine occurred, with 75% of the arginine appearing as ornithine. Similar patterns were obtained at D= 0.3 h−1, although only 30–40% of the arginine was converted to ornithine. At the 10 mM arginine level, biomass but no IG was produced while above this level, IG was also synthesised. At D= 0.3 h−1, substantial amounts of IG were detected at all arginine levels. As expected, S. mutans did not respond to increasing arginine levels and the use of this amino acid as an additional carbon and energy source with the release of ammonia in organisms such as S. milleri and Streptococcus sanguis may help to explain their dominance when growing in vitro and in vivo with S. mutans under glucose-limiting conditions. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Zilm, P.S. verfasserin aut Gully, N.J. verfasserin aut In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 37(1986), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:37 year:1986 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6968.1986.tb01757.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 37 1986 1 0 |
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10.1111/j.1574-6968.1986.tb01757.x doi (DE-627)NLEJ239760166 DE-627 ger DE-627 rakwb Rogers, A.H. verfasserin aut The utilisation of arginine by oral streptococci grown glucose-limited in a chemostat Oxford, UK Blackwell Publishing Ltd 1986 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract Pure cultures of 2 strains of Streptococcus milleri and one of Streptococcus mutans were grown glucose-limited at dilution rates of D= 0.1 h−1 and D= 0.3 h−1 and controlled pH of 7.0 in a chemically defined medium containing arginine levels of 0.6, 10, 33, 60 and 74 mM. Culture filtrate levels of arginine and its metabolites and glucose were determined as were cell dry weights and intracellular glycogen (IG).In S. milleri, stoichiometric conversion of up to 60 mM of arginine to citrulline and ornithine occurred, with 75% of the arginine appearing as ornithine. Similar patterns were obtained at D= 0.3 h−1, although only 30–40% of the arginine was converted to ornithine. At the 10 mM arginine level, biomass but no IG was produced while above this level, IG was also synthesised. At D= 0.3 h−1, substantial amounts of IG were detected at all arginine levels. As expected, S. mutans did not respond to increasing arginine levels and the use of this amino acid as an additional carbon and energy source with the release of ammonia in organisms such as S. milleri and Streptococcus sanguis may help to explain their dominance when growing in vitro and in vivo with S. mutans under glucose-limiting conditions. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Zilm, P.S. verfasserin aut Gully, N.J. verfasserin aut In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 37(1986), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:37 year:1986 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6968.1986.tb01757.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 37 1986 1 0 |
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10.1111/j.1574-6968.1986.tb01757.x doi (DE-627)NLEJ239760166 DE-627 ger DE-627 rakwb Rogers, A.H. verfasserin aut The utilisation of arginine by oral streptococci grown glucose-limited in a chemostat Oxford, UK Blackwell Publishing Ltd 1986 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract Pure cultures of 2 strains of Streptococcus milleri and one of Streptococcus mutans were grown glucose-limited at dilution rates of D= 0.1 h−1 and D= 0.3 h−1 and controlled pH of 7.0 in a chemically defined medium containing arginine levels of 0.6, 10, 33, 60 and 74 mM. Culture filtrate levels of arginine and its metabolites and glucose were determined as were cell dry weights and intracellular glycogen (IG).In S. milleri, stoichiometric conversion of up to 60 mM of arginine to citrulline and ornithine occurred, with 75% of the arginine appearing as ornithine. Similar patterns were obtained at D= 0.3 h−1, although only 30–40% of the arginine was converted to ornithine. At the 10 mM arginine level, biomass but no IG was produced while above this level, IG was also synthesised. At D= 0.3 h−1, substantial amounts of IG were detected at all arginine levels. As expected, S. mutans did not respond to increasing arginine levels and the use of this amino acid as an additional carbon and energy source with the release of ammonia in organisms such as S. milleri and Streptococcus sanguis may help to explain their dominance when growing in vitro and in vivo with S. mutans under glucose-limiting conditions. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Zilm, P.S. verfasserin aut Gully, N.J. verfasserin aut In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 37(1986), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:37 year:1986 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6968.1986.tb01757.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 37 1986 1 0 |
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10.1111/j.1574-6968.1986.tb01757.x doi (DE-627)NLEJ239760166 DE-627 ger DE-627 rakwb Rogers, A.H. verfasserin aut The utilisation of arginine by oral streptococci grown glucose-limited in a chemostat Oxford, UK Blackwell Publishing Ltd 1986 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract Pure cultures of 2 strains of Streptococcus milleri and one of Streptococcus mutans were grown glucose-limited at dilution rates of D= 0.1 h−1 and D= 0.3 h−1 and controlled pH of 7.0 in a chemically defined medium containing arginine levels of 0.6, 10, 33, 60 and 74 mM. Culture filtrate levels of arginine and its metabolites and glucose were determined as were cell dry weights and intracellular glycogen (IG).In S. milleri, stoichiometric conversion of up to 60 mM of arginine to citrulline and ornithine occurred, with 75% of the arginine appearing as ornithine. Similar patterns were obtained at D= 0.3 h−1, although only 30–40% of the arginine was converted to ornithine. At the 10 mM arginine level, biomass but no IG was produced while above this level, IG was also synthesised. At D= 0.3 h−1, substantial amounts of IG were detected at all arginine levels. As expected, S. mutans did not respond to increasing arginine levels and the use of this amino acid as an additional carbon and energy source with the release of ammonia in organisms such as S. milleri and Streptococcus sanguis may help to explain their dominance when growing in vitro and in vivo with S. mutans under glucose-limiting conditions. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Zilm, P.S. verfasserin aut Gully, N.J. verfasserin aut In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 37(1986), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:37 year:1986 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6968.1986.tb01757.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 37 1986 1 0 |
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The utilisation of arginine by oral streptococci grown glucose-limited in a chemostat |
| abstract |
Abstract Pure cultures of 2 strains of Streptococcus milleri and one of Streptococcus mutans were grown glucose-limited at dilution rates of D= 0.1 h−1 and D= 0.3 h−1 and controlled pH of 7.0 in a chemically defined medium containing arginine levels of 0.6, 10, 33, 60 and 74 mM. Culture filtrate levels of arginine and its metabolites and glucose were determined as were cell dry weights and intracellular glycogen (IG).In S. milleri, stoichiometric conversion of up to 60 mM of arginine to citrulline and ornithine occurred, with 75% of the arginine appearing as ornithine. Similar patterns were obtained at D= 0.3 h−1, although only 30–40% of the arginine was converted to ornithine. At the 10 mM arginine level, biomass but no IG was produced while above this level, IG was also synthesised. At D= 0.3 h−1, substantial amounts of IG were detected at all arginine levels. As expected, S. mutans did not respond to increasing arginine levels and the use of this amino acid as an additional carbon and energy source with the release of ammonia in organisms such as S. milleri and Streptococcus sanguis may help to explain their dominance when growing in vitro and in vivo with S. mutans under glucose-limiting conditions. |
| abstractGer |
Abstract Pure cultures of 2 strains of Streptococcus milleri and one of Streptococcus mutans were grown glucose-limited at dilution rates of D= 0.1 h−1 and D= 0.3 h−1 and controlled pH of 7.0 in a chemically defined medium containing arginine levels of 0.6, 10, 33, 60 and 74 mM. Culture filtrate levels of arginine and its metabolites and glucose were determined as were cell dry weights and intracellular glycogen (IG).In S. milleri, stoichiometric conversion of up to 60 mM of arginine to citrulline and ornithine occurred, with 75% of the arginine appearing as ornithine. Similar patterns were obtained at D= 0.3 h−1, although only 30–40% of the arginine was converted to ornithine. At the 10 mM arginine level, biomass but no IG was produced while above this level, IG was also synthesised. At D= 0.3 h−1, substantial amounts of IG were detected at all arginine levels. As expected, S. mutans did not respond to increasing arginine levels and the use of this amino acid as an additional carbon and energy source with the release of ammonia in organisms such as S. milleri and Streptococcus sanguis may help to explain their dominance when growing in vitro and in vivo with S. mutans under glucose-limiting conditions. |
| abstract_unstemmed |
Abstract Pure cultures of 2 strains of Streptococcus milleri and one of Streptococcus mutans were grown glucose-limited at dilution rates of D= 0.1 h−1 and D= 0.3 h−1 and controlled pH of 7.0 in a chemically defined medium containing arginine levels of 0.6, 10, 33, 60 and 74 mM. Culture filtrate levels of arginine and its metabolites and glucose were determined as were cell dry weights and intracellular glycogen (IG).In S. milleri, stoichiometric conversion of up to 60 mM of arginine to citrulline and ornithine occurred, with 75% of the arginine appearing as ornithine. Similar patterns were obtained at D= 0.3 h−1, although only 30–40% of the arginine was converted to ornithine. At the 10 mM arginine level, biomass but no IG was produced while above this level, IG was also synthesised. At D= 0.3 h−1, substantial amounts of IG were detected at all arginine levels. As expected, S. mutans did not respond to increasing arginine levels and the use of this amino acid as an additional carbon and energy source with the release of ammonia in organisms such as S. milleri and Streptococcus sanguis may help to explain their dominance when growing in vitro and in vivo with S. mutans under glucose-limiting conditions. |
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The utilisation of arginine by oral streptococci grown glucose-limited in a chemostat |
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Zilm, P.S. Gully, N.J. |
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