Efficiency of the transfer of a pSAM2-derivative plasmid between two strains of Streptomyces lividans in conditions ranging from agar slants to non-sterile soil microcosms
Abstract: The aim of this work was to determine the efficiency of the conjugative plasmid pTS130 to transfer in various environmental conditions between two strains of Streptomyces lividans. This plasmid is a derivative of the conjugative and integrative plasmid pSAM2 isolated originally from Strept...
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| Autor*in: |
Clerc, Sylvie [verfasserIn] Simonet, Pascal [verfasserIn] |
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E-Artikel |
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| Erschienen: |
Oxford, UK: Blackwell Publishing Ltd ; 1996 |
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| Umfang: |
Online-Ressource |
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| Reproduktion: |
2006 ; Blackwell Publishing Journal Backfiles 1879-2005 |
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| Übergeordnetes Werk: |
In: FEMS microbiology ecology - Oxford [u.a.] : Wiley-Blackwell, 1990, 21(1996), 3, Seite 0 |
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volume:21 ; year:1996 ; number:3 ; pages:0 |
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| DOI / URN: |
10.1111/j.1574-6941.1996.tb00343.x |
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| 245 | 1 | 0 | |a Efficiency of the transfer of a pSAM2-derivative plasmid between two strains of Streptomyces lividans in conditions ranging from agar slants to non-sterile soil microcosms |
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| 520 | |a Abstract: The aim of this work was to determine the efficiency of the conjugative plasmid pTS130 to transfer in various environmental conditions between two strains of Streptomyces lividans. This plasmid is a derivative of the conjugative and integrative plasmid pSAM2 isolated originally from Streptomyces ambofaciens and capable of transfer to a large range of bacteria. Our results demonstrate the high frequency of the conjugation mechanism since more than 60% of the recipient cells developed on agar slants harbored the plasmid pTS130 (as evidenced by Southern hybridization with a pSAM2 derivative plasmid probe). When donor and recipient strains were inoculated into sterile and non-sterile soil microcosms, transconjugants were detected after two days of incubation in both cases. However, the number of donor, recipient and transconjugant cells were established at a lower level in the non-sterile soil than in the sterile soil experiments. Moreover, nutrient amendment of the sterile soil was found to increase the population levels of parental strains and transfer frequencies both significantly and simultaneously. On the other hand, modifying water potential of the soil microcosms did not result in affecting the establishment of the Streptomyces lividans cells or the transfer rate. | ||
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10.1111/j.1574-6941.1996.tb00343.x doi (DE-627)NLEJ239832086 DE-627 ger DE-627 rakwb Clerc, Sylvie verfasserin aut Efficiency of the transfer of a pSAM2-derivative plasmid between two strains of Streptomyces lividans in conditions ranging from agar slants to non-sterile soil microcosms Oxford, UK Blackwell Publishing Ltd 1996 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract: The aim of this work was to determine the efficiency of the conjugative plasmid pTS130 to transfer in various environmental conditions between two strains of Streptomyces lividans. This plasmid is a derivative of the conjugative and integrative plasmid pSAM2 isolated originally from Streptomyces ambofaciens and capable of transfer to a large range of bacteria. Our results demonstrate the high frequency of the conjugation mechanism since more than 60% of the recipient cells developed on agar slants harbored the plasmid pTS130 (as evidenced by Southern hybridization with a pSAM2 derivative plasmid probe). When donor and recipient strains were inoculated into sterile and non-sterile soil microcosms, transconjugants were detected after two days of incubation in both cases. However, the number of donor, recipient and transconjugant cells were established at a lower level in the non-sterile soil than in the sterile soil experiments. Moreover, nutrient amendment of the sterile soil was found to increase the population levels of parental strains and transfer frequencies both significantly and simultaneously. On the other hand, modifying water potential of the soil microcosms did not result in affecting the establishment of the Streptomyces lividans cells or the transfer rate. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Conjugation Simonet, Pascal verfasserin aut In FEMS microbiology ecology Oxford [u.a.] : Wiley-Blackwell, 1990 21(1996), 3, Seite 0 Online-Ressource (DE-627)NLEJ243926324 (DE-600)1501712-6 1574-6941 nnns volume:21 year:1996 number:3 pages:0 http://dx.doi.org/10.1111/j.1574-6941.1996.tb00343.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 21 1996 3 0 |
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10.1111/j.1574-6941.1996.tb00343.x doi (DE-627)NLEJ239832086 DE-627 ger DE-627 rakwb Clerc, Sylvie verfasserin aut Efficiency of the transfer of a pSAM2-derivative plasmid between two strains of Streptomyces lividans in conditions ranging from agar slants to non-sterile soil microcosms Oxford, UK Blackwell Publishing Ltd 1996 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract: The aim of this work was to determine the efficiency of the conjugative plasmid pTS130 to transfer in various environmental conditions between two strains of Streptomyces lividans. This plasmid is a derivative of the conjugative and integrative plasmid pSAM2 isolated originally from Streptomyces ambofaciens and capable of transfer to a large range of bacteria. Our results demonstrate the high frequency of the conjugation mechanism since more than 60% of the recipient cells developed on agar slants harbored the plasmid pTS130 (as evidenced by Southern hybridization with a pSAM2 derivative plasmid probe). When donor and recipient strains were inoculated into sterile and non-sterile soil microcosms, transconjugants were detected after two days of incubation in both cases. However, the number of donor, recipient and transconjugant cells were established at a lower level in the non-sterile soil than in the sterile soil experiments. Moreover, nutrient amendment of the sterile soil was found to increase the population levels of parental strains and transfer frequencies both significantly and simultaneously. On the other hand, modifying water potential of the soil microcosms did not result in affecting the establishment of the Streptomyces lividans cells or the transfer rate. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Conjugation Simonet, Pascal verfasserin aut In FEMS microbiology ecology Oxford [u.a.] : Wiley-Blackwell, 1990 21(1996), 3, Seite 0 Online-Ressource (DE-627)NLEJ243926324 (DE-600)1501712-6 1574-6941 nnns volume:21 year:1996 number:3 pages:0 http://dx.doi.org/10.1111/j.1574-6941.1996.tb00343.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 21 1996 3 0 |
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10.1111/j.1574-6941.1996.tb00343.x doi (DE-627)NLEJ239832086 DE-627 ger DE-627 rakwb Clerc, Sylvie verfasserin aut Efficiency of the transfer of a pSAM2-derivative plasmid between two strains of Streptomyces lividans in conditions ranging from agar slants to non-sterile soil microcosms Oxford, UK Blackwell Publishing Ltd 1996 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract: The aim of this work was to determine the efficiency of the conjugative plasmid pTS130 to transfer in various environmental conditions between two strains of Streptomyces lividans. This plasmid is a derivative of the conjugative and integrative plasmid pSAM2 isolated originally from Streptomyces ambofaciens and capable of transfer to a large range of bacteria. Our results demonstrate the high frequency of the conjugation mechanism since more than 60% of the recipient cells developed on agar slants harbored the plasmid pTS130 (as evidenced by Southern hybridization with a pSAM2 derivative plasmid probe). When donor and recipient strains were inoculated into sterile and non-sterile soil microcosms, transconjugants were detected after two days of incubation in both cases. However, the number of donor, recipient and transconjugant cells were established at a lower level in the non-sterile soil than in the sterile soil experiments. Moreover, nutrient amendment of the sterile soil was found to increase the population levels of parental strains and transfer frequencies both significantly and simultaneously. On the other hand, modifying water potential of the soil microcosms did not result in affecting the establishment of the Streptomyces lividans cells or the transfer rate. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Conjugation Simonet, Pascal verfasserin aut In FEMS microbiology ecology Oxford [u.a.] : Wiley-Blackwell, 1990 21(1996), 3, Seite 0 Online-Ressource (DE-627)NLEJ243926324 (DE-600)1501712-6 1574-6941 nnns volume:21 year:1996 number:3 pages:0 http://dx.doi.org/10.1111/j.1574-6941.1996.tb00343.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 21 1996 3 0 |
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10.1111/j.1574-6941.1996.tb00343.x doi (DE-627)NLEJ239832086 DE-627 ger DE-627 rakwb Clerc, Sylvie verfasserin aut Efficiency of the transfer of a pSAM2-derivative plasmid between two strains of Streptomyces lividans in conditions ranging from agar slants to non-sterile soil microcosms Oxford, UK Blackwell Publishing Ltd 1996 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract: The aim of this work was to determine the efficiency of the conjugative plasmid pTS130 to transfer in various environmental conditions between two strains of Streptomyces lividans. This plasmid is a derivative of the conjugative and integrative plasmid pSAM2 isolated originally from Streptomyces ambofaciens and capable of transfer to a large range of bacteria. Our results demonstrate the high frequency of the conjugation mechanism since more than 60% of the recipient cells developed on agar slants harbored the plasmid pTS130 (as evidenced by Southern hybridization with a pSAM2 derivative plasmid probe). When donor and recipient strains were inoculated into sterile and non-sterile soil microcosms, transconjugants were detected after two days of incubation in both cases. However, the number of donor, recipient and transconjugant cells were established at a lower level in the non-sterile soil than in the sterile soil experiments. Moreover, nutrient amendment of the sterile soil was found to increase the population levels of parental strains and transfer frequencies both significantly and simultaneously. On the other hand, modifying water potential of the soil microcosms did not result in affecting the establishment of the Streptomyces lividans cells or the transfer rate. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Conjugation Simonet, Pascal verfasserin aut In FEMS microbiology ecology Oxford [u.a.] : Wiley-Blackwell, 1990 21(1996), 3, Seite 0 Online-Ressource (DE-627)NLEJ243926324 (DE-600)1501712-6 1574-6941 nnns volume:21 year:1996 number:3 pages:0 http://dx.doi.org/10.1111/j.1574-6941.1996.tb00343.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 21 1996 3 0 |
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10.1111/j.1574-6941.1996.tb00343.x doi (DE-627)NLEJ239832086 DE-627 ger DE-627 rakwb Clerc, Sylvie verfasserin aut Efficiency of the transfer of a pSAM2-derivative plasmid between two strains of Streptomyces lividans in conditions ranging from agar slants to non-sterile soil microcosms Oxford, UK Blackwell Publishing Ltd 1996 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract: The aim of this work was to determine the efficiency of the conjugative plasmid pTS130 to transfer in various environmental conditions between two strains of Streptomyces lividans. This plasmid is a derivative of the conjugative and integrative plasmid pSAM2 isolated originally from Streptomyces ambofaciens and capable of transfer to a large range of bacteria. Our results demonstrate the high frequency of the conjugation mechanism since more than 60% of the recipient cells developed on agar slants harbored the plasmid pTS130 (as evidenced by Southern hybridization with a pSAM2 derivative plasmid probe). When donor and recipient strains were inoculated into sterile and non-sterile soil microcosms, transconjugants were detected after two days of incubation in both cases. However, the number of donor, recipient and transconjugant cells were established at a lower level in the non-sterile soil than in the sterile soil experiments. Moreover, nutrient amendment of the sterile soil was found to increase the population levels of parental strains and transfer frequencies both significantly and simultaneously. On the other hand, modifying water potential of the soil microcosms did not result in affecting the establishment of the Streptomyces lividans cells or the transfer rate. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Conjugation Simonet, Pascal verfasserin aut In FEMS microbiology ecology Oxford [u.a.] : Wiley-Blackwell, 1990 21(1996), 3, Seite 0 Online-Ressource (DE-627)NLEJ243926324 (DE-600)1501712-6 1574-6941 nnns volume:21 year:1996 number:3 pages:0 http://dx.doi.org/10.1111/j.1574-6941.1996.tb00343.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 21 1996 3 0 |
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Efficiency of the transfer of a pSAM2-derivative plasmid between two strains of Streptomyces lividans in conditions ranging from agar slants to non-sterile soil microcosms Conjugation |
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efficiency of the transfer of a psam2-derivative plasmid between two strains of streptomyces lividans in conditions ranging from agar slants to non-sterile soil microcosms |
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Efficiency of the transfer of a pSAM2-derivative plasmid between two strains of Streptomyces lividans in conditions ranging from agar slants to non-sterile soil microcosms |
| abstract |
Abstract: The aim of this work was to determine the efficiency of the conjugative plasmid pTS130 to transfer in various environmental conditions between two strains of Streptomyces lividans. This plasmid is a derivative of the conjugative and integrative plasmid pSAM2 isolated originally from Streptomyces ambofaciens and capable of transfer to a large range of bacteria. Our results demonstrate the high frequency of the conjugation mechanism since more than 60% of the recipient cells developed on agar slants harbored the plasmid pTS130 (as evidenced by Southern hybridization with a pSAM2 derivative plasmid probe). When donor and recipient strains were inoculated into sterile and non-sterile soil microcosms, transconjugants were detected after two days of incubation in both cases. However, the number of donor, recipient and transconjugant cells were established at a lower level in the non-sterile soil than in the sterile soil experiments. Moreover, nutrient amendment of the sterile soil was found to increase the population levels of parental strains and transfer frequencies both significantly and simultaneously. On the other hand, modifying water potential of the soil microcosms did not result in affecting the establishment of the Streptomyces lividans cells or the transfer rate. |
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Abstract: The aim of this work was to determine the efficiency of the conjugative plasmid pTS130 to transfer in various environmental conditions between two strains of Streptomyces lividans. This plasmid is a derivative of the conjugative and integrative plasmid pSAM2 isolated originally from Streptomyces ambofaciens and capable of transfer to a large range of bacteria. Our results demonstrate the high frequency of the conjugation mechanism since more than 60% of the recipient cells developed on agar slants harbored the plasmid pTS130 (as evidenced by Southern hybridization with a pSAM2 derivative plasmid probe). When donor and recipient strains were inoculated into sterile and non-sterile soil microcosms, transconjugants were detected after two days of incubation in both cases. However, the number of donor, recipient and transconjugant cells were established at a lower level in the non-sterile soil than in the sterile soil experiments. Moreover, nutrient amendment of the sterile soil was found to increase the population levels of parental strains and transfer frequencies both significantly and simultaneously. On the other hand, modifying water potential of the soil microcosms did not result in affecting the establishment of the Streptomyces lividans cells or the transfer rate. |
| abstract_unstemmed |
Abstract: The aim of this work was to determine the efficiency of the conjugative plasmid pTS130 to transfer in various environmental conditions between two strains of Streptomyces lividans. This plasmid is a derivative of the conjugative and integrative plasmid pSAM2 isolated originally from Streptomyces ambofaciens and capable of transfer to a large range of bacteria. Our results demonstrate the high frequency of the conjugation mechanism since more than 60% of the recipient cells developed on agar slants harbored the plasmid pTS130 (as evidenced by Southern hybridization with a pSAM2 derivative plasmid probe). When donor and recipient strains were inoculated into sterile and non-sterile soil microcosms, transconjugants were detected after two days of incubation in both cases. However, the number of donor, recipient and transconjugant cells were established at a lower level in the non-sterile soil than in the sterile soil experiments. Moreover, nutrient amendment of the sterile soil was found to increase the population levels of parental strains and transfer frequencies both significantly and simultaneously. On the other hand, modifying water potential of the soil microcosms did not result in affecting the establishment of the Streptomyces lividans cells or the transfer rate. |
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Simonet, Pascal |
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NLEJ243926324 |
| mediatype_str_mv |
z |
| isOA_txt |
false |
| hochschulschrift_bool |
false |
| doi_str |
10.1111/j.1574-6941.1996.tb00343.x |
| up_date |
2024-07-06T08:29:03.555Z |
| _version_ |
1803817628263776256 |
| fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ239832086</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210707094913.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">120426s1996 xx |||||o 00| ||und c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1111/j.1574-6941.1996.tb00343.x</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ239832086</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Clerc, Sylvie</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Efficiency of the transfer of a pSAM2-derivative plasmid between two strains of Streptomyces lividans in conditions ranging from agar slants to non-sterile soil microcosms</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Oxford, UK</subfield><subfield code="b">Blackwell Publishing Ltd</subfield><subfield code="c">1996</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract: The aim of this work was to determine the efficiency of the conjugative plasmid pTS130 to transfer in various environmental conditions between two strains of Streptomyces lividans. This plasmid is a derivative of the conjugative and integrative plasmid pSAM2 isolated originally from Streptomyces ambofaciens and capable of transfer to a large range of bacteria. Our results demonstrate the high frequency of the conjugation mechanism since more than 60% of the recipient cells developed on agar slants harbored the plasmid pTS130 (as evidenced by Southern hybridization with a pSAM2 derivative plasmid probe). When donor and recipient strains were inoculated into sterile and non-sterile soil microcosms, transconjugants were detected after two days of incubation in both cases. However, the number of donor, recipient and transconjugant cells were established at a lower level in the non-sterile soil than in the sterile soil experiments. Moreover, nutrient amendment of the sterile soil was found to increase the population levels of parental strains and transfer frequencies both significantly and simultaneously. On the other hand, modifying water potential of the soil microcosms did not result in affecting the establishment of the Streptomyces lividans cells or the transfer rate.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="d">2006</subfield><subfield code="f">Blackwell Publishing Journal Backfiles 1879-2005</subfield><subfield code="7">|2006||||||||||</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Conjugation</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Simonet, Pascal</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">FEMS microbiology ecology</subfield><subfield code="d">Oxford [u.a.] : Wiley-Blackwell, 1990</subfield><subfield code="g">21(1996), 3, Seite 0</subfield><subfield code="h">Online-Ressource</subfield><subfield code="w">(DE-627)NLEJ243926324</subfield><subfield code="w">(DE-600)1501712-6</subfield><subfield code="x">1574-6941</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:21</subfield><subfield code="g">year:1996</subfield><subfield code="g">number:3</subfield><subfield code="g">pages:0</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1111/j.1574-6941.1996.tb00343.x</subfield><subfield code="q">text/html</subfield><subfield code="x">Verlag</subfield><subfield code="z">Deutschlandweit zugänglich</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-DJB</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">21</subfield><subfield code="j">1996</subfield><subfield code="e">3</subfield><subfield code="h">0</subfield></datafield></record></collection>
|
| score |
7.398546 |