Strong cytotoxicity to human gingival fibroblasts by Porphyromonas gingivalis ATCC 33277
The aim of the present study was to analyze the cytotoxicity of some bacterial species associated with periodontal diseases. The specificity of cytotoxicity was estimated against cells of various origin and from different individuals. The reference bacteria were Actinobacillus actinomycetemcoinitans...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Johansson, Anders [verfasserIn] Bergenholtz, Axel [verfasserIn] Holm, Stig E. [verfasserIn] |
|---|
| Format: |
E-Artikel |
|---|
| Erschienen: |
Oxford, UK: Blackwell Publishing Ltd ; 1996 |
|---|
| Schlagwörter: |
|---|
| Umfang: |
Online-Ressource |
|---|
| Reproduktion: |
2006 ; Blackwell Publishing Journal Backfiles 1879-2005 |
|---|---|
| Übergeordnetes Werk: |
In: Journal of periodontal research - Oxford [u.a.] : Wiley-Blackwell, 1966, 31(1996), 7, Seite 0 |
| Übergeordnetes Werk: |
volume:31 ; year:1996 ; number:7 ; pages:0 |
| Links: |
|---|
| DOI / URN: |
10.1111/j.1600-0765.1996.tb01412.x |
|---|
| Katalog-ID: |
NLEJ239992148 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLEJ239992148 | ||
| 003 | DE-627 | ||
| 005 | 20230506151109.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 120426s1996 xx |||||o 00| ||und c | ||
| 024 | 7 | |a 10.1111/j.1600-0765.1996.tb01412.x |2 doi | |
| 035 | |a (DE-627)NLEJ239992148 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 100 | 1 | |a Johansson, Anders |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Strong cytotoxicity to human gingival fibroblasts by Porphyromonas gingivalis ATCC 33277 |
| 264 | 1 | |a Oxford, UK |b Blackwell Publishing Ltd |c 1996 | |
| 300 | |a Online-Ressource | ||
| 336 | |a nicht spezifiziert |b zzz |2 rdacontent | ||
| 337 | |a nicht spezifiziert |b z |2 rdamedia | ||
| 338 | |a nicht spezifiziert |b zu |2 rdacarrier | ||
| 520 | |a The aim of the present study was to analyze the cytotoxicity of some bacterial species associated with periodontal diseases. The specificity of cytotoxicity was estimated against cells of various origin and from different individuals. The reference bacteria were Actinobacillus actinomycetemcoinitans, Porphyromonas gingivalis and Fusobacterium nucleatum. These bacteria were cultured for 24 h in liquid media and the supernatants were used in cytotoxicity assays. The target cells used were human gingival fibroblasts (GF). dermal fibroblasts (K4), gingival epithelial cells (E) and HeLa-cells (HeLa). These cells were exposed at 4 h or 24 h, respectively, to various concentrations of culture supernatants from the selected bacteria. The influence on the viability and metabolism of the cells were estimated quantitatively as increase in neutral red uptake and lactic acid production. Growth medium supernatants of P. gingivalis 33277 were strongly cytotoxic to gingival fibroblasts after 24 h incubation, compared to supernatants of P. gingivalis 381 or W 50, A. actinomycetemcoinitans or F. nucleatum cultures. The toxic effect of P. gingivalis 33277 decreased drastically after heat inactivation, which indicates effects of proteins. By adding anti-sera the cytotoxicity of P. gingivalis 33277 could be dose dependently neutralized, which was not the case when supernatants of A. actinomycetemcoinitans was tested. Target cells of epithelial origin did not show increased cytotoxicity to P. gingivalis 33277. The results of the present study strengthen the hypothesis that P. gingivalis remains as a suspect causative key component in periodontal diseases. | ||
| 533 | |d 2006 |f Blackwell Publishing Journal Backfiles 1879-2005 |7 |2006|||||||||| | ||
| 650 | 4 | |a gingival fibroblasts: Actinobacillus actinomycetemcomitans | |
| 700 | 1 | |a Bergenholtz, Axel |e verfasserin |4 aut | |
| 700 | 1 | |a Holm, Stig E. |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i In |t Journal of periodontal research |d Oxford [u.a.] : Wiley-Blackwell, 1966 |g 31(1996), 7, Seite 0 |h Online-Ressource |w (DE-627)NLEJ243927452 |w (DE-600)2025633-4 |x 1600-0765 |7 nnns |
| 773 | 1 | 8 | |g volume:31 |g year:1996 |g number:7 |g pages:0 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1111/j.1600-0765.1996.tb01412.x |q text/html |x Verlag |z Deutschlandweit zugänglich |3 Volltext |
| 912 | |a GBV_USEFLAG_U | ||
| 912 | |a ZDB-1-DJB | ||
| 912 | |a GBV_NL_ARTICLE | ||
| 951 | |a AR | ||
| 952 | |d 31 |j 1996 |e 7 |h 0 | ||
| author_variant |
a j aj a b ab s e h se seh |
|---|---|
| matchkey_str |
article:16000765:1996----::togyooiiyouagniafbolssyopyoo |
| hierarchy_sort_str |
1996 |
| publishDate |
1996 |
| allfields |
10.1111/j.1600-0765.1996.tb01412.x doi (DE-627)NLEJ239992148 DE-627 ger DE-627 rakwb Johansson, Anders verfasserin aut Strong cytotoxicity to human gingival fibroblasts by Porphyromonas gingivalis ATCC 33277 Oxford, UK Blackwell Publishing Ltd 1996 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The aim of the present study was to analyze the cytotoxicity of some bacterial species associated with periodontal diseases. The specificity of cytotoxicity was estimated against cells of various origin and from different individuals. The reference bacteria were Actinobacillus actinomycetemcoinitans, Porphyromonas gingivalis and Fusobacterium nucleatum. These bacteria were cultured for 24 h in liquid media and the supernatants were used in cytotoxicity assays. The target cells used were human gingival fibroblasts (GF). dermal fibroblasts (K4), gingival epithelial cells (E) and HeLa-cells (HeLa). These cells were exposed at 4 h or 24 h, respectively, to various concentrations of culture supernatants from the selected bacteria. The influence on the viability and metabolism of the cells were estimated quantitatively as increase in neutral red uptake and lactic acid production. Growth medium supernatants of P. gingivalis 33277 were strongly cytotoxic to gingival fibroblasts after 24 h incubation, compared to supernatants of P. gingivalis 381 or W 50, A. actinomycetemcoinitans or F. nucleatum cultures. The toxic effect of P. gingivalis 33277 decreased drastically after heat inactivation, which indicates effects of proteins. By adding anti-sera the cytotoxicity of P. gingivalis 33277 could be dose dependently neutralized, which was not the case when supernatants of A. actinomycetemcoinitans was tested. Target cells of epithelial origin did not show increased cytotoxicity to P. gingivalis 33277. The results of the present study strengthen the hypothesis that P. gingivalis remains as a suspect causative key component in periodontal diseases. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| gingival fibroblasts: Actinobacillus actinomycetemcomitans Bergenholtz, Axel verfasserin aut Holm, Stig E. verfasserin aut In Journal of periodontal research Oxford [u.a.] : Wiley-Blackwell, 1966 31(1996), 7, Seite 0 Online-Ressource (DE-627)NLEJ243927452 (DE-600)2025633-4 1600-0765 nnns volume:31 year:1996 number:7 pages:0 http://dx.doi.org/10.1111/j.1600-0765.1996.tb01412.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 31 1996 7 0 |
| spelling |
10.1111/j.1600-0765.1996.tb01412.x doi (DE-627)NLEJ239992148 DE-627 ger DE-627 rakwb Johansson, Anders verfasserin aut Strong cytotoxicity to human gingival fibroblasts by Porphyromonas gingivalis ATCC 33277 Oxford, UK Blackwell Publishing Ltd 1996 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The aim of the present study was to analyze the cytotoxicity of some bacterial species associated with periodontal diseases. The specificity of cytotoxicity was estimated against cells of various origin and from different individuals. The reference bacteria were Actinobacillus actinomycetemcoinitans, Porphyromonas gingivalis and Fusobacterium nucleatum. These bacteria were cultured for 24 h in liquid media and the supernatants were used in cytotoxicity assays. The target cells used were human gingival fibroblasts (GF). dermal fibroblasts (K4), gingival epithelial cells (E) and HeLa-cells (HeLa). These cells were exposed at 4 h or 24 h, respectively, to various concentrations of culture supernatants from the selected bacteria. The influence on the viability and metabolism of the cells were estimated quantitatively as increase in neutral red uptake and lactic acid production. Growth medium supernatants of P. gingivalis 33277 were strongly cytotoxic to gingival fibroblasts after 24 h incubation, compared to supernatants of P. gingivalis 381 or W 50, A. actinomycetemcoinitans or F. nucleatum cultures. The toxic effect of P. gingivalis 33277 decreased drastically after heat inactivation, which indicates effects of proteins. By adding anti-sera the cytotoxicity of P. gingivalis 33277 could be dose dependently neutralized, which was not the case when supernatants of A. actinomycetemcoinitans was tested. Target cells of epithelial origin did not show increased cytotoxicity to P. gingivalis 33277. The results of the present study strengthen the hypothesis that P. gingivalis remains as a suspect causative key component in periodontal diseases. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| gingival fibroblasts: Actinobacillus actinomycetemcomitans Bergenholtz, Axel verfasserin aut Holm, Stig E. verfasserin aut In Journal of periodontal research Oxford [u.a.] : Wiley-Blackwell, 1966 31(1996), 7, Seite 0 Online-Ressource (DE-627)NLEJ243927452 (DE-600)2025633-4 1600-0765 nnns volume:31 year:1996 number:7 pages:0 http://dx.doi.org/10.1111/j.1600-0765.1996.tb01412.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 31 1996 7 0 |
| allfields_unstemmed |
10.1111/j.1600-0765.1996.tb01412.x doi (DE-627)NLEJ239992148 DE-627 ger DE-627 rakwb Johansson, Anders verfasserin aut Strong cytotoxicity to human gingival fibroblasts by Porphyromonas gingivalis ATCC 33277 Oxford, UK Blackwell Publishing Ltd 1996 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The aim of the present study was to analyze the cytotoxicity of some bacterial species associated with periodontal diseases. The specificity of cytotoxicity was estimated against cells of various origin and from different individuals. The reference bacteria were Actinobacillus actinomycetemcoinitans, Porphyromonas gingivalis and Fusobacterium nucleatum. These bacteria were cultured for 24 h in liquid media and the supernatants were used in cytotoxicity assays. The target cells used were human gingival fibroblasts (GF). dermal fibroblasts (K4), gingival epithelial cells (E) and HeLa-cells (HeLa). These cells were exposed at 4 h or 24 h, respectively, to various concentrations of culture supernatants from the selected bacteria. The influence on the viability and metabolism of the cells were estimated quantitatively as increase in neutral red uptake and lactic acid production. Growth medium supernatants of P. gingivalis 33277 were strongly cytotoxic to gingival fibroblasts after 24 h incubation, compared to supernatants of P. gingivalis 381 or W 50, A. actinomycetemcoinitans or F. nucleatum cultures. The toxic effect of P. gingivalis 33277 decreased drastically after heat inactivation, which indicates effects of proteins. By adding anti-sera the cytotoxicity of P. gingivalis 33277 could be dose dependently neutralized, which was not the case when supernatants of A. actinomycetemcoinitans was tested. Target cells of epithelial origin did not show increased cytotoxicity to P. gingivalis 33277. The results of the present study strengthen the hypothesis that P. gingivalis remains as a suspect causative key component in periodontal diseases. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| gingival fibroblasts: Actinobacillus actinomycetemcomitans Bergenholtz, Axel verfasserin aut Holm, Stig E. verfasserin aut In Journal of periodontal research Oxford [u.a.] : Wiley-Blackwell, 1966 31(1996), 7, Seite 0 Online-Ressource (DE-627)NLEJ243927452 (DE-600)2025633-4 1600-0765 nnns volume:31 year:1996 number:7 pages:0 http://dx.doi.org/10.1111/j.1600-0765.1996.tb01412.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 31 1996 7 0 |
| allfieldsGer |
10.1111/j.1600-0765.1996.tb01412.x doi (DE-627)NLEJ239992148 DE-627 ger DE-627 rakwb Johansson, Anders verfasserin aut Strong cytotoxicity to human gingival fibroblasts by Porphyromonas gingivalis ATCC 33277 Oxford, UK Blackwell Publishing Ltd 1996 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The aim of the present study was to analyze the cytotoxicity of some bacterial species associated with periodontal diseases. The specificity of cytotoxicity was estimated against cells of various origin and from different individuals. The reference bacteria were Actinobacillus actinomycetemcoinitans, Porphyromonas gingivalis and Fusobacterium nucleatum. These bacteria were cultured for 24 h in liquid media and the supernatants were used in cytotoxicity assays. The target cells used were human gingival fibroblasts (GF). dermal fibroblasts (K4), gingival epithelial cells (E) and HeLa-cells (HeLa). These cells were exposed at 4 h or 24 h, respectively, to various concentrations of culture supernatants from the selected bacteria. The influence on the viability and metabolism of the cells were estimated quantitatively as increase in neutral red uptake and lactic acid production. Growth medium supernatants of P. gingivalis 33277 were strongly cytotoxic to gingival fibroblasts after 24 h incubation, compared to supernatants of P. gingivalis 381 or W 50, A. actinomycetemcoinitans or F. nucleatum cultures. The toxic effect of P. gingivalis 33277 decreased drastically after heat inactivation, which indicates effects of proteins. By adding anti-sera the cytotoxicity of P. gingivalis 33277 could be dose dependently neutralized, which was not the case when supernatants of A. actinomycetemcoinitans was tested. Target cells of epithelial origin did not show increased cytotoxicity to P. gingivalis 33277. The results of the present study strengthen the hypothesis that P. gingivalis remains as a suspect causative key component in periodontal diseases. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| gingival fibroblasts: Actinobacillus actinomycetemcomitans Bergenholtz, Axel verfasserin aut Holm, Stig E. verfasserin aut In Journal of periodontal research Oxford [u.a.] : Wiley-Blackwell, 1966 31(1996), 7, Seite 0 Online-Ressource (DE-627)NLEJ243927452 (DE-600)2025633-4 1600-0765 nnns volume:31 year:1996 number:7 pages:0 http://dx.doi.org/10.1111/j.1600-0765.1996.tb01412.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 31 1996 7 0 |
| allfieldsSound |
10.1111/j.1600-0765.1996.tb01412.x doi (DE-627)NLEJ239992148 DE-627 ger DE-627 rakwb Johansson, Anders verfasserin aut Strong cytotoxicity to human gingival fibroblasts by Porphyromonas gingivalis ATCC 33277 Oxford, UK Blackwell Publishing Ltd 1996 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The aim of the present study was to analyze the cytotoxicity of some bacterial species associated with periodontal diseases. The specificity of cytotoxicity was estimated against cells of various origin and from different individuals. The reference bacteria were Actinobacillus actinomycetemcoinitans, Porphyromonas gingivalis and Fusobacterium nucleatum. These bacteria were cultured for 24 h in liquid media and the supernatants were used in cytotoxicity assays. The target cells used were human gingival fibroblasts (GF). dermal fibroblasts (K4), gingival epithelial cells (E) and HeLa-cells (HeLa). These cells were exposed at 4 h or 24 h, respectively, to various concentrations of culture supernatants from the selected bacteria. The influence on the viability and metabolism of the cells were estimated quantitatively as increase in neutral red uptake and lactic acid production. Growth medium supernatants of P. gingivalis 33277 were strongly cytotoxic to gingival fibroblasts after 24 h incubation, compared to supernatants of P. gingivalis 381 or W 50, A. actinomycetemcoinitans or F. nucleatum cultures. The toxic effect of P. gingivalis 33277 decreased drastically after heat inactivation, which indicates effects of proteins. By adding anti-sera the cytotoxicity of P. gingivalis 33277 could be dose dependently neutralized, which was not the case when supernatants of A. actinomycetemcoinitans was tested. Target cells of epithelial origin did not show increased cytotoxicity to P. gingivalis 33277. The results of the present study strengthen the hypothesis that P. gingivalis remains as a suspect causative key component in periodontal diseases. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| gingival fibroblasts: Actinobacillus actinomycetemcomitans Bergenholtz, Axel verfasserin aut Holm, Stig E. verfasserin aut In Journal of periodontal research Oxford [u.a.] : Wiley-Blackwell, 1966 31(1996), 7, Seite 0 Online-Ressource (DE-627)NLEJ243927452 (DE-600)2025633-4 1600-0765 nnns volume:31 year:1996 number:7 pages:0 http://dx.doi.org/10.1111/j.1600-0765.1996.tb01412.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 31 1996 7 0 |
| source |
In Journal of periodontal research 31(1996), 7, Seite 0 volume:31 year:1996 number:7 pages:0 |
| sourceStr |
In Journal of periodontal research 31(1996), 7, Seite 0 volume:31 year:1996 number:7 pages:0 |
| format_phy_str_mv |
Article |
| institution |
findex.gbv.de |
| topic_facet |
gingival fibroblasts: Actinobacillus actinomycetemcomitans |
| isfreeaccess_bool |
false |
| container_title |
Journal of periodontal research |
| authorswithroles_txt_mv |
Johansson, Anders @@aut@@ Bergenholtz, Axel @@aut@@ Holm, Stig E. @@aut@@ |
| publishDateDaySort_date |
1996-01-01T00:00:00Z |
| hierarchy_top_id |
NLEJ243927452 |
| id |
NLEJ239992148 |
| fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ239992148</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230506151109.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">120426s1996 xx |||||o 00| ||und c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1111/j.1600-0765.1996.tb01412.x</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ239992148</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Johansson, Anders</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Strong cytotoxicity to human gingival fibroblasts by Porphyromonas gingivalis ATCC 33277</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Oxford, UK</subfield><subfield code="b">Blackwell Publishing Ltd</subfield><subfield code="c">1996</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">The aim of the present study was to analyze the cytotoxicity of some bacterial species associated with periodontal diseases. The specificity of cytotoxicity was estimated against cells of various origin and from different individuals. The reference bacteria were Actinobacillus actinomycetemcoinitans, Porphyromonas gingivalis and Fusobacterium nucleatum. These bacteria were cultured for 24 h in liquid media and the supernatants were used in cytotoxicity assays. The target cells used were human gingival fibroblasts (GF). dermal fibroblasts (K4), gingival epithelial cells (E) and HeLa-cells (HeLa). These cells were exposed at 4 h or 24 h, respectively, to various concentrations of culture supernatants from the selected bacteria. The influence on the viability and metabolism of the cells were estimated quantitatively as increase in neutral red uptake and lactic acid production. Growth medium supernatants of P. gingivalis 33277 were strongly cytotoxic to gingival fibroblasts after 24 h incubation, compared to supernatants of P. gingivalis 381 or W 50, A. actinomycetemcoinitans or F. nucleatum cultures. The toxic effect of P. gingivalis 33277 decreased drastically after heat inactivation, which indicates effects of proteins. By adding anti-sera the cytotoxicity of P. gingivalis 33277 could be dose dependently neutralized, which was not the case when supernatants of A. actinomycetemcoinitans was tested. Target cells of epithelial origin did not show increased cytotoxicity to P. gingivalis 33277. The results of the present study strengthen the hypothesis that P. gingivalis remains as a suspect causative key component in periodontal diseases.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="d">2006</subfield><subfield code="f">Blackwell Publishing Journal Backfiles 1879-2005</subfield><subfield code="7">|2006||||||||||</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">gingival fibroblasts: Actinobacillus actinomycetemcomitans</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Bergenholtz, Axel</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Holm, Stig E.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Journal of periodontal research</subfield><subfield code="d">Oxford [u.a.] : Wiley-Blackwell, 1966</subfield><subfield code="g">31(1996), 7, Seite 0</subfield><subfield code="h">Online-Ressource</subfield><subfield code="w">(DE-627)NLEJ243927452</subfield><subfield code="w">(DE-600)2025633-4</subfield><subfield code="x">1600-0765</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:31</subfield><subfield code="g">year:1996</subfield><subfield code="g">number:7</subfield><subfield code="g">pages:0</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1111/j.1600-0765.1996.tb01412.x</subfield><subfield code="q">text/html</subfield><subfield code="x">Verlag</subfield><subfield code="z">Deutschlandweit zugänglich</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-DJB</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">31</subfield><subfield code="j">1996</subfield><subfield code="e">7</subfield><subfield code="h">0</subfield></datafield></record></collection>
|
| series2 |
Blackwell Publishing Journal Backfiles 1879-2005 |
| author |
Johansson, Anders |
| spellingShingle |
Johansson, Anders misc gingival fibroblasts: Actinobacillus actinomycetemcomitans Strong cytotoxicity to human gingival fibroblasts by Porphyromonas gingivalis ATCC 33277 |
| authorStr |
Johansson, Anders |
| ppnlink_with_tag_str_mv |
@@773@@(DE-627)NLEJ243927452 |
| format |
electronic Article |
| delete_txt_mv |
keep |
| author_role |
aut aut aut |
| collection |
NL |
| publishPlace |
Oxford, UK |
| remote_str |
true |
| illustrated |
Not Illustrated |
| issn |
1600-0765 |
| topic_title |
Strong cytotoxicity to human gingival fibroblasts by Porphyromonas gingivalis ATCC 33277 gingival fibroblasts: Actinobacillus actinomycetemcomitans |
| publisher |
Blackwell Publishing Ltd |
| publisherStr |
Blackwell Publishing Ltd |
| topic |
misc gingival fibroblasts: Actinobacillus actinomycetemcomitans |
| topic_unstemmed |
misc gingival fibroblasts: Actinobacillus actinomycetemcomitans |
| topic_browse |
misc gingival fibroblasts: Actinobacillus actinomycetemcomitans |
| format_facet |
Elektronische Aufsätze Aufsätze Elektronische Ressource |
| format_main_str_mv |
Text Zeitschrift/Artikel |
| carriertype_str_mv |
zu |
| hierarchy_parent_title |
Journal of periodontal research |
| hierarchy_parent_id |
NLEJ243927452 |
| hierarchy_top_title |
Journal of periodontal research |
| isfreeaccess_txt |
false |
| familylinks_str_mv |
(DE-627)NLEJ243927452 (DE-600)2025633-4 |
| title |
Strong cytotoxicity to human gingival fibroblasts by Porphyromonas gingivalis ATCC 33277 |
| ctrlnum |
(DE-627)NLEJ239992148 |
| title_full |
Strong cytotoxicity to human gingival fibroblasts by Porphyromonas gingivalis ATCC 33277 |
| author_sort |
Johansson, Anders |
| journal |
Journal of periodontal research |
| journalStr |
Journal of periodontal research |
| isOA_bool |
false |
| recordtype |
marc |
| publishDateSort |
1996 |
| contenttype_str_mv |
zzz |
| container_start_page |
0 |
| author_browse |
Johansson, Anders Bergenholtz, Axel Holm, Stig E. |
| container_volume |
31 |
| physical |
Online-Ressource |
| format_se |
Elektronische Aufsätze |
| author-letter |
Johansson, Anders |
| doi_str_mv |
10.1111/j.1600-0765.1996.tb01412.x |
| author2-role |
verfasserin |
| title_sort |
strong cytotoxicity to human gingival fibroblasts by porphyromonas gingivalis atcc 33277 |
| title_auth |
Strong cytotoxicity to human gingival fibroblasts by Porphyromonas gingivalis ATCC 33277 |
| abstract |
The aim of the present study was to analyze the cytotoxicity of some bacterial species associated with periodontal diseases. The specificity of cytotoxicity was estimated against cells of various origin and from different individuals. The reference bacteria were Actinobacillus actinomycetemcoinitans, Porphyromonas gingivalis and Fusobacterium nucleatum. These bacteria were cultured for 24 h in liquid media and the supernatants were used in cytotoxicity assays. The target cells used were human gingival fibroblasts (GF). dermal fibroblasts (K4), gingival epithelial cells (E) and HeLa-cells (HeLa). These cells were exposed at 4 h or 24 h, respectively, to various concentrations of culture supernatants from the selected bacteria. The influence on the viability and metabolism of the cells were estimated quantitatively as increase in neutral red uptake and lactic acid production. Growth medium supernatants of P. gingivalis 33277 were strongly cytotoxic to gingival fibroblasts after 24 h incubation, compared to supernatants of P. gingivalis 381 or W 50, A. actinomycetemcoinitans or F. nucleatum cultures. The toxic effect of P. gingivalis 33277 decreased drastically after heat inactivation, which indicates effects of proteins. By adding anti-sera the cytotoxicity of P. gingivalis 33277 could be dose dependently neutralized, which was not the case when supernatants of A. actinomycetemcoinitans was tested. Target cells of epithelial origin did not show increased cytotoxicity to P. gingivalis 33277. The results of the present study strengthen the hypothesis that P. gingivalis remains as a suspect causative key component in periodontal diseases. |
| abstractGer |
The aim of the present study was to analyze the cytotoxicity of some bacterial species associated with periodontal diseases. The specificity of cytotoxicity was estimated against cells of various origin and from different individuals. The reference bacteria were Actinobacillus actinomycetemcoinitans, Porphyromonas gingivalis and Fusobacterium nucleatum. These bacteria were cultured for 24 h in liquid media and the supernatants were used in cytotoxicity assays. The target cells used were human gingival fibroblasts (GF). dermal fibroblasts (K4), gingival epithelial cells (E) and HeLa-cells (HeLa). These cells were exposed at 4 h or 24 h, respectively, to various concentrations of culture supernatants from the selected bacteria. The influence on the viability and metabolism of the cells were estimated quantitatively as increase in neutral red uptake and lactic acid production. Growth medium supernatants of P. gingivalis 33277 were strongly cytotoxic to gingival fibroblasts after 24 h incubation, compared to supernatants of P. gingivalis 381 or W 50, A. actinomycetemcoinitans or F. nucleatum cultures. The toxic effect of P. gingivalis 33277 decreased drastically after heat inactivation, which indicates effects of proteins. By adding anti-sera the cytotoxicity of P. gingivalis 33277 could be dose dependently neutralized, which was not the case when supernatants of A. actinomycetemcoinitans was tested. Target cells of epithelial origin did not show increased cytotoxicity to P. gingivalis 33277. The results of the present study strengthen the hypothesis that P. gingivalis remains as a suspect causative key component in periodontal diseases. |
| abstract_unstemmed |
The aim of the present study was to analyze the cytotoxicity of some bacterial species associated with periodontal diseases. The specificity of cytotoxicity was estimated against cells of various origin and from different individuals. The reference bacteria were Actinobacillus actinomycetemcoinitans, Porphyromonas gingivalis and Fusobacterium nucleatum. These bacteria were cultured for 24 h in liquid media and the supernatants were used in cytotoxicity assays. The target cells used were human gingival fibroblasts (GF). dermal fibroblasts (K4), gingival epithelial cells (E) and HeLa-cells (HeLa). These cells were exposed at 4 h or 24 h, respectively, to various concentrations of culture supernatants from the selected bacteria. The influence on the viability and metabolism of the cells were estimated quantitatively as increase in neutral red uptake and lactic acid production. Growth medium supernatants of P. gingivalis 33277 were strongly cytotoxic to gingival fibroblasts after 24 h incubation, compared to supernatants of P. gingivalis 381 or W 50, A. actinomycetemcoinitans or F. nucleatum cultures. The toxic effect of P. gingivalis 33277 decreased drastically after heat inactivation, which indicates effects of proteins. By adding anti-sera the cytotoxicity of P. gingivalis 33277 could be dose dependently neutralized, which was not the case when supernatants of A. actinomycetemcoinitans was tested. Target cells of epithelial origin did not show increased cytotoxicity to P. gingivalis 33277. The results of the present study strengthen the hypothesis that P. gingivalis remains as a suspect causative key component in periodontal diseases. |
| collection_details |
GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE |
| container_issue |
7 |
| title_short |
Strong cytotoxicity to human gingival fibroblasts by Porphyromonas gingivalis ATCC 33277 |
| url |
http://dx.doi.org/10.1111/j.1600-0765.1996.tb01412.x |
| remote_bool |
true |
| author2 |
Bergenholtz, Axel Holm, Stig E. |
| author2Str |
Bergenholtz, Axel Holm, Stig E. |
| ppnlink |
NLEJ243927452 |
| mediatype_str_mv |
z |
| isOA_txt |
false |
| hochschulschrift_bool |
false |
| doi_str |
10.1111/j.1600-0765.1996.tb01412.x |
| up_date |
2024-07-06T08:51:11.852Z |
| _version_ |
1803819021084131328 |
| fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ239992148</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230506151109.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">120426s1996 xx |||||o 00| ||und c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1111/j.1600-0765.1996.tb01412.x</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ239992148</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Johansson, Anders</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Strong cytotoxicity to human gingival fibroblasts by Porphyromonas gingivalis ATCC 33277</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Oxford, UK</subfield><subfield code="b">Blackwell Publishing Ltd</subfield><subfield code="c">1996</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">The aim of the present study was to analyze the cytotoxicity of some bacterial species associated with periodontal diseases. The specificity of cytotoxicity was estimated against cells of various origin and from different individuals. The reference bacteria were Actinobacillus actinomycetemcoinitans, Porphyromonas gingivalis and Fusobacterium nucleatum. These bacteria were cultured for 24 h in liquid media and the supernatants were used in cytotoxicity assays. The target cells used were human gingival fibroblasts (GF). dermal fibroblasts (K4), gingival epithelial cells (E) and HeLa-cells (HeLa). These cells were exposed at 4 h or 24 h, respectively, to various concentrations of culture supernatants from the selected bacteria. The influence on the viability and metabolism of the cells were estimated quantitatively as increase in neutral red uptake and lactic acid production. Growth medium supernatants of P. gingivalis 33277 were strongly cytotoxic to gingival fibroblasts after 24 h incubation, compared to supernatants of P. gingivalis 381 or W 50, A. actinomycetemcoinitans or F. nucleatum cultures. The toxic effect of P. gingivalis 33277 decreased drastically after heat inactivation, which indicates effects of proteins. By adding anti-sera the cytotoxicity of P. gingivalis 33277 could be dose dependently neutralized, which was not the case when supernatants of A. actinomycetemcoinitans was tested. Target cells of epithelial origin did not show increased cytotoxicity to P. gingivalis 33277. The results of the present study strengthen the hypothesis that P. gingivalis remains as a suspect causative key component in periodontal diseases.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="d">2006</subfield><subfield code="f">Blackwell Publishing Journal Backfiles 1879-2005</subfield><subfield code="7">|2006||||||||||</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">gingival fibroblasts: Actinobacillus actinomycetemcomitans</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Bergenholtz, Axel</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Holm, Stig E.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Journal of periodontal research</subfield><subfield code="d">Oxford [u.a.] : Wiley-Blackwell, 1966</subfield><subfield code="g">31(1996), 7, Seite 0</subfield><subfield code="h">Online-Ressource</subfield><subfield code="w">(DE-627)NLEJ243927452</subfield><subfield code="w">(DE-600)2025633-4</subfield><subfield code="x">1600-0765</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:31</subfield><subfield code="g">year:1996</subfield><subfield code="g">number:7</subfield><subfield code="g">pages:0</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1111/j.1600-0765.1996.tb01412.x</subfield><subfield code="q">text/html</subfield><subfield code="x">Verlag</subfield><subfield code="z">Deutschlandweit zugänglich</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-DJB</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">31</subfield><subfield code="j">1996</subfield><subfield code="e">7</subfield><subfield code="h">0</subfield></datafield></record></collection>
|
| score |
7.4000654 |