Quantitation and optimization of enzymatic and mechanical procedures to produce high-yield single cell suspensions from human gingiva
Existing enzymatic and mechanical techniques of cell release from human gingiva were quantitated in regard to: cell yield; viability; freedom from cell clumping; and debris formation. To optimize these parameters methodologies were modified and combined with new procedures which included incubation...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
McCulloch, C. A. G. [verfasserIn] Knowles, G. [verfasserIn] Overall, C. M. [verfasserIn] |
|---|
| Format: |
E-Artikel |
|---|
| Erschienen: |
Oxford, UK: Blackwell Publishing Ltd ; 1987 |
|---|
| Umfang: |
Online-Ressource |
|---|
| Reproduktion: |
2006 ; Blackwell Publishing Journal Backfiles 1879-2005 |
|---|---|
| Übergeordnetes Werk: |
In: Journal of periodontal research - Oxford [u.a.] : Wiley-Blackwell, 1966, 22(1987), 1, Seite 0 |
| Übergeordnetes Werk: |
volume:22 ; year:1987 ; number:1 ; pages:0 |
| Links: |
|---|
| DOI / URN: |
10.1111/j.1600-0765.1987.tb01538.x |
|---|
| Katalog-ID: |
NLEJ239999770 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLEJ239999770 | ||
| 003 | DE-627 | ||
| 005 | 20210707101343.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 120426s1987 xx |||||o 00| ||und c | ||
| 024 | 7 | |a 10.1111/j.1600-0765.1987.tb01538.x |2 doi | |
| 035 | |a (DE-627)NLEJ239999770 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 100 | 1 | |a McCulloch, C. A. G. |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Quantitation and optimization of enzymatic and mechanical procedures to produce high-yield single cell suspensions from human gingiva |
| 264 | 1 | |a Oxford, UK |b Blackwell Publishing Ltd |c 1987 | |
| 300 | |a Online-Ressource | ||
| 336 | |a nicht spezifiziert |b zzz |2 rdacontent | ||
| 337 | |a nicht spezifiziert |b z |2 rdamedia | ||
| 338 | |a nicht spezifiziert |b zu |2 rdacarrier | ||
| 520 | |a Existing enzymatic and mechanical techniques of cell release from human gingiva were quantitated in regard to: cell yield; viability; freedom from cell clumping; and debris formation. To optimize these parameters methodologies were modified and combined with new procedures which included incubation with a variety of enzymes. Fibrous and inflamed tissues from 17 patients were examined. The optimal procedure that was selected included thorough mincing of the tissue plus the addition of DNA-ase, elastase and hyaluronidase to a standard bacterial collagenase mixture. Consistently, up to 3.4 × 106 cells per 100 mg of tissue were released after tissue digestion, with a mean viability of 85%. The cell suspensions were relatively free of debris and cell clumps. Data from Coulter particle counting was in close agreement with hemocytometer counts. The composition of the cell suspension was not significantly different from the original tissue sample. Tissue remnants following the digestion procedure only contained an estimated 1 % of the original number of cells in the sample. The single cell suspensions produced using this method were found to be suitable for analysis by flow cytometry. The possible selective cytotoxicity of the enzymatic technique on cell populations in the DNA-synthesis phase of the cell cycle was examined in healthy gingival tissue of a Cynomolgus monkey. The gingiva was injected with 3H-thymidine and the percentage of labeled fibroblasts was enumerated in radioautographs either of sections prepared from undigested tissue or in smears of single cell suspensions. There was no significant difference in the percentage of labeled cells between sections or smears. The data suggest that the method reported here can be applied to flow cytometry analysis of progenitor cell populations in gingival tissues. | ||
| 533 | |d 2006 |f Blackwell Publishing Journal Backfiles 1879-2005 |7 |2006|||||||||| | ||
| 700 | 1 | |a Knowles, G. |e verfasserin |4 aut | |
| 700 | 1 | |a Overall, C. M. |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i In |t Journal of periodontal research |d Oxford [u.a.] : Wiley-Blackwell, 1966 |g 22(1987), 1, Seite 0 |h Online-Ressource |w (DE-627)NLEJ243927452 |w (DE-600)2025633-4 |x 1600-0765 |7 nnns |
| 773 | 1 | 8 | |g volume:22 |g year:1987 |g number:1 |g pages:0 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1111/j.1600-0765.1987.tb01538.x |q text/html |x Verlag |z Deutschlandweit zugänglich |3 Volltext |
| 912 | |a GBV_USEFLAG_U | ||
| 912 | |a ZDB-1-DJB | ||
| 912 | |a GBV_NL_ARTICLE | ||
| 951 | |a AR | ||
| 952 | |d 22 |j 1987 |e 1 |h 0 | ||
| author_variant |
c a g m cag cagm g k gk c m o cm cmo |
|---|---|
| matchkey_str |
article:16000765:1987----::uniainnotmztooezmtcnmcaiapoeuetpoueihilsnlc |
| hierarchy_sort_str |
1987 |
| publishDate |
1987 |
| allfields |
10.1111/j.1600-0765.1987.tb01538.x doi (DE-627)NLEJ239999770 DE-627 ger DE-627 rakwb McCulloch, C. A. G. verfasserin aut Quantitation and optimization of enzymatic and mechanical procedures to produce high-yield single cell suspensions from human gingiva Oxford, UK Blackwell Publishing Ltd 1987 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Existing enzymatic and mechanical techniques of cell release from human gingiva were quantitated in regard to: cell yield; viability; freedom from cell clumping; and debris formation. To optimize these parameters methodologies were modified and combined with new procedures which included incubation with a variety of enzymes. Fibrous and inflamed tissues from 17 patients were examined. The optimal procedure that was selected included thorough mincing of the tissue plus the addition of DNA-ase, elastase and hyaluronidase to a standard bacterial collagenase mixture. Consistently, up to 3.4 × 106 cells per 100 mg of tissue were released after tissue digestion, with a mean viability of 85%. The cell suspensions were relatively free of debris and cell clumps. Data from Coulter particle counting was in close agreement with hemocytometer counts. The composition of the cell suspension was not significantly different from the original tissue sample. Tissue remnants following the digestion procedure only contained an estimated 1 % of the original number of cells in the sample. The single cell suspensions produced using this method were found to be suitable for analysis by flow cytometry. The possible selective cytotoxicity of the enzymatic technique on cell populations in the DNA-synthesis phase of the cell cycle was examined in healthy gingival tissue of a Cynomolgus monkey. The gingiva was injected with 3H-thymidine and the percentage of labeled fibroblasts was enumerated in radioautographs either of sections prepared from undigested tissue or in smears of single cell suspensions. There was no significant difference in the percentage of labeled cells between sections or smears. The data suggest that the method reported here can be applied to flow cytometry analysis of progenitor cell populations in gingival tissues. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Knowles, G. verfasserin aut Overall, C. M. verfasserin aut In Journal of periodontal research Oxford [u.a.] : Wiley-Blackwell, 1966 22(1987), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927452 (DE-600)2025633-4 1600-0765 nnns volume:22 year:1987 number:1 pages:0 http://dx.doi.org/10.1111/j.1600-0765.1987.tb01538.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 22 1987 1 0 |
| spelling |
10.1111/j.1600-0765.1987.tb01538.x doi (DE-627)NLEJ239999770 DE-627 ger DE-627 rakwb McCulloch, C. A. G. verfasserin aut Quantitation and optimization of enzymatic and mechanical procedures to produce high-yield single cell suspensions from human gingiva Oxford, UK Blackwell Publishing Ltd 1987 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Existing enzymatic and mechanical techniques of cell release from human gingiva were quantitated in regard to: cell yield; viability; freedom from cell clumping; and debris formation. To optimize these parameters methodologies were modified and combined with new procedures which included incubation with a variety of enzymes. Fibrous and inflamed tissues from 17 patients were examined. The optimal procedure that was selected included thorough mincing of the tissue plus the addition of DNA-ase, elastase and hyaluronidase to a standard bacterial collagenase mixture. Consistently, up to 3.4 × 106 cells per 100 mg of tissue were released after tissue digestion, with a mean viability of 85%. The cell suspensions were relatively free of debris and cell clumps. Data from Coulter particle counting was in close agreement with hemocytometer counts. The composition of the cell suspension was not significantly different from the original tissue sample. Tissue remnants following the digestion procedure only contained an estimated 1 % of the original number of cells in the sample. The single cell suspensions produced using this method were found to be suitable for analysis by flow cytometry. The possible selective cytotoxicity of the enzymatic technique on cell populations in the DNA-synthesis phase of the cell cycle was examined in healthy gingival tissue of a Cynomolgus monkey. The gingiva was injected with 3H-thymidine and the percentage of labeled fibroblasts was enumerated in radioautographs either of sections prepared from undigested tissue or in smears of single cell suspensions. There was no significant difference in the percentage of labeled cells between sections or smears. The data suggest that the method reported here can be applied to flow cytometry analysis of progenitor cell populations in gingival tissues. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Knowles, G. verfasserin aut Overall, C. M. verfasserin aut In Journal of periodontal research Oxford [u.a.] : Wiley-Blackwell, 1966 22(1987), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927452 (DE-600)2025633-4 1600-0765 nnns volume:22 year:1987 number:1 pages:0 http://dx.doi.org/10.1111/j.1600-0765.1987.tb01538.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 22 1987 1 0 |
| allfields_unstemmed |
10.1111/j.1600-0765.1987.tb01538.x doi (DE-627)NLEJ239999770 DE-627 ger DE-627 rakwb McCulloch, C. A. G. verfasserin aut Quantitation and optimization of enzymatic and mechanical procedures to produce high-yield single cell suspensions from human gingiva Oxford, UK Blackwell Publishing Ltd 1987 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Existing enzymatic and mechanical techniques of cell release from human gingiva were quantitated in regard to: cell yield; viability; freedom from cell clumping; and debris formation. To optimize these parameters methodologies were modified and combined with new procedures which included incubation with a variety of enzymes. Fibrous and inflamed tissues from 17 patients were examined. The optimal procedure that was selected included thorough mincing of the tissue plus the addition of DNA-ase, elastase and hyaluronidase to a standard bacterial collagenase mixture. Consistently, up to 3.4 × 106 cells per 100 mg of tissue were released after tissue digestion, with a mean viability of 85%. The cell suspensions were relatively free of debris and cell clumps. Data from Coulter particle counting was in close agreement with hemocytometer counts. The composition of the cell suspension was not significantly different from the original tissue sample. Tissue remnants following the digestion procedure only contained an estimated 1 % of the original number of cells in the sample. The single cell suspensions produced using this method were found to be suitable for analysis by flow cytometry. The possible selective cytotoxicity of the enzymatic technique on cell populations in the DNA-synthesis phase of the cell cycle was examined in healthy gingival tissue of a Cynomolgus monkey. The gingiva was injected with 3H-thymidine and the percentage of labeled fibroblasts was enumerated in radioautographs either of sections prepared from undigested tissue or in smears of single cell suspensions. There was no significant difference in the percentage of labeled cells between sections or smears. The data suggest that the method reported here can be applied to flow cytometry analysis of progenitor cell populations in gingival tissues. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Knowles, G. verfasserin aut Overall, C. M. verfasserin aut In Journal of periodontal research Oxford [u.a.] : Wiley-Blackwell, 1966 22(1987), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927452 (DE-600)2025633-4 1600-0765 nnns volume:22 year:1987 number:1 pages:0 http://dx.doi.org/10.1111/j.1600-0765.1987.tb01538.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 22 1987 1 0 |
| allfieldsGer |
10.1111/j.1600-0765.1987.tb01538.x doi (DE-627)NLEJ239999770 DE-627 ger DE-627 rakwb McCulloch, C. A. G. verfasserin aut Quantitation and optimization of enzymatic and mechanical procedures to produce high-yield single cell suspensions from human gingiva Oxford, UK Blackwell Publishing Ltd 1987 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Existing enzymatic and mechanical techniques of cell release from human gingiva were quantitated in regard to: cell yield; viability; freedom from cell clumping; and debris formation. To optimize these parameters methodologies were modified and combined with new procedures which included incubation with a variety of enzymes. Fibrous and inflamed tissues from 17 patients were examined. The optimal procedure that was selected included thorough mincing of the tissue plus the addition of DNA-ase, elastase and hyaluronidase to a standard bacterial collagenase mixture. Consistently, up to 3.4 × 106 cells per 100 mg of tissue were released after tissue digestion, with a mean viability of 85%. The cell suspensions were relatively free of debris and cell clumps. Data from Coulter particle counting was in close agreement with hemocytometer counts. The composition of the cell suspension was not significantly different from the original tissue sample. Tissue remnants following the digestion procedure only contained an estimated 1 % of the original number of cells in the sample. The single cell suspensions produced using this method were found to be suitable for analysis by flow cytometry. The possible selective cytotoxicity of the enzymatic technique on cell populations in the DNA-synthesis phase of the cell cycle was examined in healthy gingival tissue of a Cynomolgus monkey. The gingiva was injected with 3H-thymidine and the percentage of labeled fibroblasts was enumerated in radioautographs either of sections prepared from undigested tissue or in smears of single cell suspensions. There was no significant difference in the percentage of labeled cells between sections or smears. The data suggest that the method reported here can be applied to flow cytometry analysis of progenitor cell populations in gingival tissues. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Knowles, G. verfasserin aut Overall, C. M. verfasserin aut In Journal of periodontal research Oxford [u.a.] : Wiley-Blackwell, 1966 22(1987), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927452 (DE-600)2025633-4 1600-0765 nnns volume:22 year:1987 number:1 pages:0 http://dx.doi.org/10.1111/j.1600-0765.1987.tb01538.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 22 1987 1 0 |
| allfieldsSound |
10.1111/j.1600-0765.1987.tb01538.x doi (DE-627)NLEJ239999770 DE-627 ger DE-627 rakwb McCulloch, C. A. G. verfasserin aut Quantitation and optimization of enzymatic and mechanical procedures to produce high-yield single cell suspensions from human gingiva Oxford, UK Blackwell Publishing Ltd 1987 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Existing enzymatic and mechanical techniques of cell release from human gingiva were quantitated in regard to: cell yield; viability; freedom from cell clumping; and debris formation. To optimize these parameters methodologies were modified and combined with new procedures which included incubation with a variety of enzymes. Fibrous and inflamed tissues from 17 patients were examined. The optimal procedure that was selected included thorough mincing of the tissue plus the addition of DNA-ase, elastase and hyaluronidase to a standard bacterial collagenase mixture. Consistently, up to 3.4 × 106 cells per 100 mg of tissue were released after tissue digestion, with a mean viability of 85%. The cell suspensions were relatively free of debris and cell clumps. Data from Coulter particle counting was in close agreement with hemocytometer counts. The composition of the cell suspension was not significantly different from the original tissue sample. Tissue remnants following the digestion procedure only contained an estimated 1 % of the original number of cells in the sample. The single cell suspensions produced using this method were found to be suitable for analysis by flow cytometry. The possible selective cytotoxicity of the enzymatic technique on cell populations in the DNA-synthesis phase of the cell cycle was examined in healthy gingival tissue of a Cynomolgus monkey. The gingiva was injected with 3H-thymidine and the percentage of labeled fibroblasts was enumerated in radioautographs either of sections prepared from undigested tissue or in smears of single cell suspensions. There was no significant difference in the percentage of labeled cells between sections or smears. The data suggest that the method reported here can be applied to flow cytometry analysis of progenitor cell populations in gingival tissues. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Knowles, G. verfasserin aut Overall, C. M. verfasserin aut In Journal of periodontal research Oxford [u.a.] : Wiley-Blackwell, 1966 22(1987), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927452 (DE-600)2025633-4 1600-0765 nnns volume:22 year:1987 number:1 pages:0 http://dx.doi.org/10.1111/j.1600-0765.1987.tb01538.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 22 1987 1 0 |
| source |
In Journal of periodontal research 22(1987), 1, Seite 0 volume:22 year:1987 number:1 pages:0 |
| sourceStr |
In Journal of periodontal research 22(1987), 1, Seite 0 volume:22 year:1987 number:1 pages:0 |
| format_phy_str_mv |
Article |
| institution |
findex.gbv.de |
| isfreeaccess_bool |
false |
| container_title |
Journal of periodontal research |
| authorswithroles_txt_mv |
McCulloch, C. A. G. @@aut@@ Knowles, G. @@aut@@ Overall, C. M. @@aut@@ |
| publishDateDaySort_date |
1987-01-01T00:00:00Z |
| hierarchy_top_id |
NLEJ243927452 |
| id |
NLEJ239999770 |
| fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ239999770</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210707101343.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">120426s1987 xx |||||o 00| ||und c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1111/j.1600-0765.1987.tb01538.x</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ239999770</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">McCulloch, C. A. G.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Quantitation and optimization of enzymatic and mechanical procedures to produce high-yield single cell suspensions from human gingiva</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Oxford, UK</subfield><subfield code="b">Blackwell Publishing Ltd</subfield><subfield code="c">1987</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Existing enzymatic and mechanical techniques of cell release from human gingiva were quantitated in regard to: cell yield; viability; freedom from cell clumping; and debris formation. To optimize these parameters methodologies were modified and combined with new procedures which included incubation with a variety of enzymes. Fibrous and inflamed tissues from 17 patients were examined. The optimal procedure that was selected included thorough mincing of the tissue plus the addition of DNA-ase, elastase and hyaluronidase to a standard bacterial collagenase mixture. Consistently, up to 3.4 × 106 cells per 100 mg of tissue were released after tissue digestion, with a mean viability of 85%. The cell suspensions were relatively free of debris and cell clumps. Data from Coulter particle counting was in close agreement with hemocytometer counts. The composition of the cell suspension was not significantly different from the original tissue sample. Tissue remnants following the digestion procedure only contained an estimated 1 % of the original number of cells in the sample. The single cell suspensions produced using this method were found to be suitable for analysis by flow cytometry. The possible selective cytotoxicity of the enzymatic technique on cell populations in the DNA-synthesis phase of the cell cycle was examined in healthy gingival tissue of a Cynomolgus monkey. The gingiva was injected with 3H-thymidine and the percentage of labeled fibroblasts was enumerated in radioautographs either of sections prepared from undigested tissue or in smears of single cell suspensions. There was no significant difference in the percentage of labeled cells between sections or smears. The data suggest that the method reported here can be applied to flow cytometry analysis of progenitor cell populations in gingival tissues.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="d">2006</subfield><subfield code="f">Blackwell Publishing Journal Backfiles 1879-2005</subfield><subfield code="7">|2006||||||||||</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Knowles, G.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Overall, C. M.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Journal of periodontal research</subfield><subfield code="d">Oxford [u.a.] : Wiley-Blackwell, 1966</subfield><subfield code="g">22(1987), 1, Seite 0</subfield><subfield code="h">Online-Ressource</subfield><subfield code="w">(DE-627)NLEJ243927452</subfield><subfield code="w">(DE-600)2025633-4</subfield><subfield code="x">1600-0765</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:22</subfield><subfield code="g">year:1987</subfield><subfield code="g">number:1</subfield><subfield code="g">pages:0</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1111/j.1600-0765.1987.tb01538.x</subfield><subfield code="q">text/html</subfield><subfield code="x">Verlag</subfield><subfield code="z">Deutschlandweit zugänglich</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-DJB</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">22</subfield><subfield code="j">1987</subfield><subfield code="e">1</subfield><subfield code="h">0</subfield></datafield></record></collection>
|
| series2 |
Blackwell Publishing Journal Backfiles 1879-2005 |
| author |
McCulloch, C. A. G. |
| spellingShingle |
McCulloch, C. A. G. Quantitation and optimization of enzymatic and mechanical procedures to produce high-yield single cell suspensions from human gingiva |
| authorStr |
McCulloch, C. A. G. |
| ppnlink_with_tag_str_mv |
@@773@@(DE-627)NLEJ243927452 |
| format |
electronic Article |
| delete_txt_mv |
keep |
| author_role |
aut aut aut |
| collection |
NL |
| publishPlace |
Oxford, UK |
| remote_str |
true |
| illustrated |
Not Illustrated |
| issn |
1600-0765 |
| topic_title |
Quantitation and optimization of enzymatic and mechanical procedures to produce high-yield single cell suspensions from human gingiva |
| publisher |
Blackwell Publishing Ltd |
| publisherStr |
Blackwell Publishing Ltd |
| format_facet |
Elektronische Aufsätze Aufsätze Elektronische Ressource |
| format_main_str_mv |
Text Zeitschrift/Artikel |
| carriertype_str_mv |
zu |
| hierarchy_parent_title |
Journal of periodontal research |
| hierarchy_parent_id |
NLEJ243927452 |
| hierarchy_top_title |
Journal of periodontal research |
| isfreeaccess_txt |
false |
| familylinks_str_mv |
(DE-627)NLEJ243927452 (DE-600)2025633-4 |
| title |
Quantitation and optimization of enzymatic and mechanical procedures to produce high-yield single cell suspensions from human gingiva |
| ctrlnum |
(DE-627)NLEJ239999770 |
| title_full |
Quantitation and optimization of enzymatic and mechanical procedures to produce high-yield single cell suspensions from human gingiva |
| author_sort |
McCulloch, C. A. G. |
| journal |
Journal of periodontal research |
| journalStr |
Journal of periodontal research |
| isOA_bool |
false |
| recordtype |
marc |
| publishDateSort |
1987 |
| contenttype_str_mv |
zzz |
| container_start_page |
0 |
| author_browse |
McCulloch, C. A. G. Knowles, G. Overall, C. M. |
| container_volume |
22 |
| physical |
Online-Ressource |
| format_se |
Elektronische Aufsätze |
| author-letter |
McCulloch, C. A. G. |
| doi_str_mv |
10.1111/j.1600-0765.1987.tb01538.x |
| author2-role |
verfasserin |
| title_sort |
quantitation and optimization of enzymatic and mechanical procedures to produce high-yield single cell suspensions from human gingiva |
| title_auth |
Quantitation and optimization of enzymatic and mechanical procedures to produce high-yield single cell suspensions from human gingiva |
| abstract |
Existing enzymatic and mechanical techniques of cell release from human gingiva were quantitated in regard to: cell yield; viability; freedom from cell clumping; and debris formation. To optimize these parameters methodologies were modified and combined with new procedures which included incubation with a variety of enzymes. Fibrous and inflamed tissues from 17 patients were examined. The optimal procedure that was selected included thorough mincing of the tissue plus the addition of DNA-ase, elastase and hyaluronidase to a standard bacterial collagenase mixture. Consistently, up to 3.4 × 106 cells per 100 mg of tissue were released after tissue digestion, with a mean viability of 85%. The cell suspensions were relatively free of debris and cell clumps. Data from Coulter particle counting was in close agreement with hemocytometer counts. The composition of the cell suspension was not significantly different from the original tissue sample. Tissue remnants following the digestion procedure only contained an estimated 1 % of the original number of cells in the sample. The single cell suspensions produced using this method were found to be suitable for analysis by flow cytometry. The possible selective cytotoxicity of the enzymatic technique on cell populations in the DNA-synthesis phase of the cell cycle was examined in healthy gingival tissue of a Cynomolgus monkey. The gingiva was injected with 3H-thymidine and the percentage of labeled fibroblasts was enumerated in radioautographs either of sections prepared from undigested tissue or in smears of single cell suspensions. There was no significant difference in the percentage of labeled cells between sections or smears. The data suggest that the method reported here can be applied to flow cytometry analysis of progenitor cell populations in gingival tissues. |
| abstractGer |
Existing enzymatic and mechanical techniques of cell release from human gingiva were quantitated in regard to: cell yield; viability; freedom from cell clumping; and debris formation. To optimize these parameters methodologies were modified and combined with new procedures which included incubation with a variety of enzymes. Fibrous and inflamed tissues from 17 patients were examined. The optimal procedure that was selected included thorough mincing of the tissue plus the addition of DNA-ase, elastase and hyaluronidase to a standard bacterial collagenase mixture. Consistently, up to 3.4 × 106 cells per 100 mg of tissue were released after tissue digestion, with a mean viability of 85%. The cell suspensions were relatively free of debris and cell clumps. Data from Coulter particle counting was in close agreement with hemocytometer counts. The composition of the cell suspension was not significantly different from the original tissue sample. Tissue remnants following the digestion procedure only contained an estimated 1 % of the original number of cells in the sample. The single cell suspensions produced using this method were found to be suitable for analysis by flow cytometry. The possible selective cytotoxicity of the enzymatic technique on cell populations in the DNA-synthesis phase of the cell cycle was examined in healthy gingival tissue of a Cynomolgus monkey. The gingiva was injected with 3H-thymidine and the percentage of labeled fibroblasts was enumerated in radioautographs either of sections prepared from undigested tissue or in smears of single cell suspensions. There was no significant difference in the percentage of labeled cells between sections or smears. The data suggest that the method reported here can be applied to flow cytometry analysis of progenitor cell populations in gingival tissues. |
| abstract_unstemmed |
Existing enzymatic and mechanical techniques of cell release from human gingiva were quantitated in regard to: cell yield; viability; freedom from cell clumping; and debris formation. To optimize these parameters methodologies were modified and combined with new procedures which included incubation with a variety of enzymes. Fibrous and inflamed tissues from 17 patients were examined. The optimal procedure that was selected included thorough mincing of the tissue plus the addition of DNA-ase, elastase and hyaluronidase to a standard bacterial collagenase mixture. Consistently, up to 3.4 × 106 cells per 100 mg of tissue were released after tissue digestion, with a mean viability of 85%. The cell suspensions were relatively free of debris and cell clumps. Data from Coulter particle counting was in close agreement with hemocytometer counts. The composition of the cell suspension was not significantly different from the original tissue sample. Tissue remnants following the digestion procedure only contained an estimated 1 % of the original number of cells in the sample. The single cell suspensions produced using this method were found to be suitable for analysis by flow cytometry. The possible selective cytotoxicity of the enzymatic technique on cell populations in the DNA-synthesis phase of the cell cycle was examined in healthy gingival tissue of a Cynomolgus monkey. The gingiva was injected with 3H-thymidine and the percentage of labeled fibroblasts was enumerated in radioautographs either of sections prepared from undigested tissue or in smears of single cell suspensions. There was no significant difference in the percentage of labeled cells between sections or smears. The data suggest that the method reported here can be applied to flow cytometry analysis of progenitor cell populations in gingival tissues. |
| collection_details |
GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE |
| container_issue |
1 |
| title_short |
Quantitation and optimization of enzymatic and mechanical procedures to produce high-yield single cell suspensions from human gingiva |
| url |
http://dx.doi.org/10.1111/j.1600-0765.1987.tb01538.x |
| remote_bool |
true |
| author2 |
Knowles, G. Overall, C. M. |
| author2Str |
Knowles, G. Overall, C. M. |
| ppnlink |
NLEJ243927452 |
| mediatype_str_mv |
z |
| isOA_txt |
false |
| hochschulschrift_bool |
false |
| doi_str |
10.1111/j.1600-0765.1987.tb01538.x |
| up_date |
2024-07-06T08:52:39.832Z |
| _version_ |
1803819113337847808 |
| fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ239999770</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210707101343.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">120426s1987 xx |||||o 00| ||und c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1111/j.1600-0765.1987.tb01538.x</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ239999770</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">McCulloch, C. A. G.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Quantitation and optimization of enzymatic and mechanical procedures to produce high-yield single cell suspensions from human gingiva</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Oxford, UK</subfield><subfield code="b">Blackwell Publishing Ltd</subfield><subfield code="c">1987</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Existing enzymatic and mechanical techniques of cell release from human gingiva were quantitated in regard to: cell yield; viability; freedom from cell clumping; and debris formation. To optimize these parameters methodologies were modified and combined with new procedures which included incubation with a variety of enzymes. Fibrous and inflamed tissues from 17 patients were examined. The optimal procedure that was selected included thorough mincing of the tissue plus the addition of DNA-ase, elastase and hyaluronidase to a standard bacterial collagenase mixture. Consistently, up to 3.4 × 106 cells per 100 mg of tissue were released after tissue digestion, with a mean viability of 85%. The cell suspensions were relatively free of debris and cell clumps. Data from Coulter particle counting was in close agreement with hemocytometer counts. The composition of the cell suspension was not significantly different from the original tissue sample. Tissue remnants following the digestion procedure only contained an estimated 1 % of the original number of cells in the sample. The single cell suspensions produced using this method were found to be suitable for analysis by flow cytometry. The possible selective cytotoxicity of the enzymatic technique on cell populations in the DNA-synthesis phase of the cell cycle was examined in healthy gingival tissue of a Cynomolgus monkey. The gingiva was injected with 3H-thymidine and the percentage of labeled fibroblasts was enumerated in radioautographs either of sections prepared from undigested tissue or in smears of single cell suspensions. There was no significant difference in the percentage of labeled cells between sections or smears. The data suggest that the method reported here can be applied to flow cytometry analysis of progenitor cell populations in gingival tissues.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="d">2006</subfield><subfield code="f">Blackwell Publishing Journal Backfiles 1879-2005</subfield><subfield code="7">|2006||||||||||</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Knowles, G.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Overall, C. M.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Journal of periodontal research</subfield><subfield code="d">Oxford [u.a.] : Wiley-Blackwell, 1966</subfield><subfield code="g">22(1987), 1, Seite 0</subfield><subfield code="h">Online-Ressource</subfield><subfield code="w">(DE-627)NLEJ243927452</subfield><subfield code="w">(DE-600)2025633-4</subfield><subfield code="x">1600-0765</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:22</subfield><subfield code="g">year:1987</subfield><subfield code="g">number:1</subfield><subfield code="g">pages:0</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1111/j.1600-0765.1987.tb01538.x</subfield><subfield code="q">text/html</subfield><subfield code="x">Verlag</subfield><subfield code="z">Deutschlandweit zugänglich</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-DJB</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">22</subfield><subfield code="j">1987</subfield><subfield code="e">1</subfield><subfield code="h">0</subfield></datafield></record></collection>
|
| score |
7.400939 |