Con A stimulation of collagenase synthesis by human gingival fibroblasts
Presence of 10–1000 μg/ml of Con A in cultures of human gingival fibroblasts promoted the synthesis and secretion of collagenase. Incubation with Con A for a minimum of 6 hours was required for the effect which could be inhibited over this time period by the addition of α-methyl mannoside. In the co...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Wang, H.-M. [verfasserIn] Hurum, S. [verfasserIn] Sodek, J. [verfasserIn] |
|---|
| Format: |
E-Artikel |
|---|
| Erschienen: |
Oxford, UK: Blackwell Publishing Ltd ; 1983 |
|---|
| Umfang: |
Online-Ressource |
|---|
| Reproduktion: |
2006 ; Blackwell Publishing Journal Backfiles 1879-2005 |
|---|---|
| Übergeordnetes Werk: |
In: Journal of periodontal research - Oxford [u.a.] : Wiley-Blackwell, 1966, 18(1983), 2, Seite 0 |
| Übergeordnetes Werk: |
volume:18 ; year:1983 ; number:2 ; pages:0 |
| Links: |
|---|
| DOI / URN: |
10.1111/j.1600-0765.1983.tb00347.x |
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| 520 | |a Presence of 10–1000 μg/ml of Con A in cultures of human gingival fibroblasts promoted the synthesis and secretion of collagenase. Incubation with Con A for a minimum of 6 hours was required for the effect which could be inhibited over this time period by the addition of α-methyl mannoside. In the continuous presence of Con A, a latent period of at least 8 hours was required before the stimulating effect was observed. Con A also stimulated the synthesis of a number of other unidentified proteins secreted into the medium. Of other lectins tested, phytohemagglutinin produced a moderate stimulation of collagenase production, but only a small effect was observed with pokeweed mitogen and wheat germ agglutinin. The enzyme, which was detected almost entirely in a latent form, was shown to be a true collagenase through the production of typical 3/4- and 1/4- fragments from a collagen substrate at neutral pH and the inhibition of activity by sulfhydryl compounds and metal chelators. | ||
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10.1111/j.1600-0765.1983.tb00347.x doi (DE-627)NLEJ240003357 DE-627 ger DE-627 rakwb Wang, H.-M. verfasserin aut Con A stimulation of collagenase synthesis by human gingival fibroblasts Oxford, UK Blackwell Publishing Ltd 1983 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Presence of 10–1000 μg/ml of Con A in cultures of human gingival fibroblasts promoted the synthesis and secretion of collagenase. Incubation with Con A for a minimum of 6 hours was required for the effect which could be inhibited over this time period by the addition of α-methyl mannoside. In the continuous presence of Con A, a latent period of at least 8 hours was required before the stimulating effect was observed. Con A also stimulated the synthesis of a number of other unidentified proteins secreted into the medium. Of other lectins tested, phytohemagglutinin produced a moderate stimulation of collagenase production, but only a small effect was observed with pokeweed mitogen and wheat germ agglutinin. The enzyme, which was detected almost entirely in a latent form, was shown to be a true collagenase through the production of typical 3/4- and 1/4- fragments from a collagen substrate at neutral pH and the inhibition of activity by sulfhydryl compounds and metal chelators. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Hurum, S. verfasserin aut Sodek, J. verfasserin aut In Journal of periodontal research Oxford [u.a.] : Wiley-Blackwell, 1966 18(1983), 2, Seite 0 Online-Ressource (DE-627)NLEJ243927452 (DE-600)2025633-4 1600-0765 nnns volume:18 year:1983 number:2 pages:0 http://dx.doi.org/10.1111/j.1600-0765.1983.tb00347.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 18 1983 2 0 |
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10.1111/j.1600-0765.1983.tb00347.x doi (DE-627)NLEJ240003357 DE-627 ger DE-627 rakwb Wang, H.-M. verfasserin aut Con A stimulation of collagenase synthesis by human gingival fibroblasts Oxford, UK Blackwell Publishing Ltd 1983 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Presence of 10–1000 μg/ml of Con A in cultures of human gingival fibroblasts promoted the synthesis and secretion of collagenase. Incubation with Con A for a minimum of 6 hours was required for the effect which could be inhibited over this time period by the addition of α-methyl mannoside. In the continuous presence of Con A, a latent period of at least 8 hours was required before the stimulating effect was observed. Con A also stimulated the synthesis of a number of other unidentified proteins secreted into the medium. Of other lectins tested, phytohemagglutinin produced a moderate stimulation of collagenase production, but only a small effect was observed with pokeweed mitogen and wheat germ agglutinin. The enzyme, which was detected almost entirely in a latent form, was shown to be a true collagenase through the production of typical 3/4- and 1/4- fragments from a collagen substrate at neutral pH and the inhibition of activity by sulfhydryl compounds and metal chelators. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Hurum, S. verfasserin aut Sodek, J. verfasserin aut In Journal of periodontal research Oxford [u.a.] : Wiley-Blackwell, 1966 18(1983), 2, Seite 0 Online-Ressource (DE-627)NLEJ243927452 (DE-600)2025633-4 1600-0765 nnns volume:18 year:1983 number:2 pages:0 http://dx.doi.org/10.1111/j.1600-0765.1983.tb00347.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 18 1983 2 0 |
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10.1111/j.1600-0765.1983.tb00347.x doi (DE-627)NLEJ240003357 DE-627 ger DE-627 rakwb Wang, H.-M. verfasserin aut Con A stimulation of collagenase synthesis by human gingival fibroblasts Oxford, UK Blackwell Publishing Ltd 1983 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Presence of 10–1000 μg/ml of Con A in cultures of human gingival fibroblasts promoted the synthesis and secretion of collagenase. Incubation with Con A for a minimum of 6 hours was required for the effect which could be inhibited over this time period by the addition of α-methyl mannoside. In the continuous presence of Con A, a latent period of at least 8 hours was required before the stimulating effect was observed. Con A also stimulated the synthesis of a number of other unidentified proteins secreted into the medium. Of other lectins tested, phytohemagglutinin produced a moderate stimulation of collagenase production, but only a small effect was observed with pokeweed mitogen and wheat germ agglutinin. The enzyme, which was detected almost entirely in a latent form, was shown to be a true collagenase through the production of typical 3/4- and 1/4- fragments from a collagen substrate at neutral pH and the inhibition of activity by sulfhydryl compounds and metal chelators. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Hurum, S. verfasserin aut Sodek, J. verfasserin aut In Journal of periodontal research Oxford [u.a.] : Wiley-Blackwell, 1966 18(1983), 2, Seite 0 Online-Ressource (DE-627)NLEJ243927452 (DE-600)2025633-4 1600-0765 nnns volume:18 year:1983 number:2 pages:0 http://dx.doi.org/10.1111/j.1600-0765.1983.tb00347.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 18 1983 2 0 |
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10.1111/j.1600-0765.1983.tb00347.x doi (DE-627)NLEJ240003357 DE-627 ger DE-627 rakwb Wang, H.-M. verfasserin aut Con A stimulation of collagenase synthesis by human gingival fibroblasts Oxford, UK Blackwell Publishing Ltd 1983 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Presence of 10–1000 μg/ml of Con A in cultures of human gingival fibroblasts promoted the synthesis and secretion of collagenase. Incubation with Con A for a minimum of 6 hours was required for the effect which could be inhibited over this time period by the addition of α-methyl mannoside. In the continuous presence of Con A, a latent period of at least 8 hours was required before the stimulating effect was observed. Con A also stimulated the synthesis of a number of other unidentified proteins secreted into the medium. Of other lectins tested, phytohemagglutinin produced a moderate stimulation of collagenase production, but only a small effect was observed with pokeweed mitogen and wheat germ agglutinin. The enzyme, which was detected almost entirely in a latent form, was shown to be a true collagenase through the production of typical 3/4- and 1/4- fragments from a collagen substrate at neutral pH and the inhibition of activity by sulfhydryl compounds and metal chelators. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Hurum, S. verfasserin aut Sodek, J. verfasserin aut In Journal of periodontal research Oxford [u.a.] : Wiley-Blackwell, 1966 18(1983), 2, Seite 0 Online-Ressource (DE-627)NLEJ243927452 (DE-600)2025633-4 1600-0765 nnns volume:18 year:1983 number:2 pages:0 http://dx.doi.org/10.1111/j.1600-0765.1983.tb00347.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 18 1983 2 0 |
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10.1111/j.1600-0765.1983.tb00347.x doi (DE-627)NLEJ240003357 DE-627 ger DE-627 rakwb Wang, H.-M. verfasserin aut Con A stimulation of collagenase synthesis by human gingival fibroblasts Oxford, UK Blackwell Publishing Ltd 1983 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Presence of 10–1000 μg/ml of Con A in cultures of human gingival fibroblasts promoted the synthesis and secretion of collagenase. Incubation with Con A for a minimum of 6 hours was required for the effect which could be inhibited over this time period by the addition of α-methyl mannoside. In the continuous presence of Con A, a latent period of at least 8 hours was required before the stimulating effect was observed. Con A also stimulated the synthesis of a number of other unidentified proteins secreted into the medium. Of other lectins tested, phytohemagglutinin produced a moderate stimulation of collagenase production, but only a small effect was observed with pokeweed mitogen and wheat germ agglutinin. The enzyme, which was detected almost entirely in a latent form, was shown to be a true collagenase through the production of typical 3/4- and 1/4- fragments from a collagen substrate at neutral pH and the inhibition of activity by sulfhydryl compounds and metal chelators. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Hurum, S. verfasserin aut Sodek, J. verfasserin aut In Journal of periodontal research Oxford [u.a.] : Wiley-Blackwell, 1966 18(1983), 2, Seite 0 Online-Ressource (DE-627)NLEJ243927452 (DE-600)2025633-4 1600-0765 nnns volume:18 year:1983 number:2 pages:0 http://dx.doi.org/10.1111/j.1600-0765.1983.tb00347.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 18 1983 2 0 |
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Presence of 10–1000 μg/ml of Con A in cultures of human gingival fibroblasts promoted the synthesis and secretion of collagenase. Incubation with Con A for a minimum of 6 hours was required for the effect which could be inhibited over this time period by the addition of α-methyl mannoside. In the continuous presence of Con A, a latent period of at least 8 hours was required before the stimulating effect was observed. Con A also stimulated the synthesis of a number of other unidentified proteins secreted into the medium. Of other lectins tested, phytohemagglutinin produced a moderate stimulation of collagenase production, but only a small effect was observed with pokeweed mitogen and wheat germ agglutinin. The enzyme, which was detected almost entirely in a latent form, was shown to be a true collagenase through the production of typical 3/4- and 1/4- fragments from a collagen substrate at neutral pH and the inhibition of activity by sulfhydryl compounds and metal chelators. |
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Presence of 10–1000 μg/ml of Con A in cultures of human gingival fibroblasts promoted the synthesis and secretion of collagenase. Incubation with Con A for a minimum of 6 hours was required for the effect which could be inhibited over this time period by the addition of α-methyl mannoside. In the continuous presence of Con A, a latent period of at least 8 hours was required before the stimulating effect was observed. Con A also stimulated the synthesis of a number of other unidentified proteins secreted into the medium. Of other lectins tested, phytohemagglutinin produced a moderate stimulation of collagenase production, but only a small effect was observed with pokeweed mitogen and wheat germ agglutinin. The enzyme, which was detected almost entirely in a latent form, was shown to be a true collagenase through the production of typical 3/4- and 1/4- fragments from a collagen substrate at neutral pH and the inhibition of activity by sulfhydryl compounds and metal chelators. |
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Presence of 10–1000 μg/ml of Con A in cultures of human gingival fibroblasts promoted the synthesis and secretion of collagenase. Incubation with Con A for a minimum of 6 hours was required for the effect which could be inhibited over this time period by the addition of α-methyl mannoside. In the continuous presence of Con A, a latent period of at least 8 hours was required before the stimulating effect was observed. Con A also stimulated the synthesis of a number of other unidentified proteins secreted into the medium. Of other lectins tested, phytohemagglutinin produced a moderate stimulation of collagenase production, but only a small effect was observed with pokeweed mitogen and wheat germ agglutinin. The enzyme, which was detected almost entirely in a latent form, was shown to be a true collagenase through the production of typical 3/4- and 1/4- fragments from a collagen substrate at neutral pH and the inhibition of activity by sulfhydryl compounds and metal chelators. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ240003357</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210707101411.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">120426s1983 xx |||||o 00| ||und c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1111/j.1600-0765.1983.tb00347.x</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ240003357</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Wang, H.-M.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Con A stimulation of collagenase synthesis by human gingival fibroblasts</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Oxford, UK</subfield><subfield code="b">Blackwell Publishing Ltd</subfield><subfield code="c">1983</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Presence of 10–1000 μg/ml of Con A in cultures of human gingival fibroblasts promoted the synthesis and secretion of collagenase. Incubation with Con A for a minimum of 6 hours was required for the effect which could be inhibited over this time period by the addition of α-methyl mannoside. In the continuous presence of Con A, a latent period of at least 8 hours was required before the stimulating effect was observed. Con A also stimulated the synthesis of a number of other unidentified proteins secreted into the medium. Of other lectins tested, phytohemagglutinin produced a moderate stimulation of collagenase production, but only a small effect was observed with pokeweed mitogen and wheat germ agglutinin. The enzyme, which was detected almost entirely in a latent form, was shown to be a true collagenase through the production of typical 3/4- and 1/4- fragments from a collagen substrate at neutral pH and the inhibition of activity by sulfhydryl compounds and metal chelators.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="d">2006</subfield><subfield code="f">Blackwell Publishing Journal Backfiles 1879-2005</subfield><subfield code="7">|2006||||||||||</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Hurum, S.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Sodek, J.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Journal of periodontal research</subfield><subfield code="d">Oxford [u.a.] : Wiley-Blackwell, 1966</subfield><subfield code="g">18(1983), 2, Seite 0</subfield><subfield code="h">Online-Ressource</subfield><subfield code="w">(DE-627)NLEJ243927452</subfield><subfield code="w">(DE-600)2025633-4</subfield><subfield code="x">1600-0765</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:18</subfield><subfield code="g">year:1983</subfield><subfield code="g">number:2</subfield><subfield code="g">pages:0</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1111/j.1600-0765.1983.tb00347.x</subfield><subfield code="q">text/html</subfield><subfield code="x">Verlag</subfield><subfield code="z">Deutschlandweit zugänglich</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-DJB</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">18</subfield><subfield code="j">1983</subfield><subfield code="e">2</subfield><subfield code="h">0</subfield></datafield></record></collection>
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