Heterogeneity in the Expression Pattern of Two Ganglioside Synthase Genes During Mouse Brain Development
Abstract: Gangliosides are synthesized by sequential catalytic reaction of multiple glycosyltransferases. GM2/GD2 synthase and GD3 synthase are key enzymes for ganglioside synthesis, because their relative activities regulate the main profiles of ganglioside expression. Mouse GD3 synthase (EC 2.4.99...
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E-Artikel |
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Oxford, UK: Blackwell Science Ltd ; 1996 |
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Online-Ressource |
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2002 ; Blackwell Publishing Journal Backfiles 1879-2005 |
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In: Journal of neurochemistry - Oxford : Wiley-Blackwell, 1956, 66(1996), 1, Seite 0 |
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volume:66 ; year:1996 ; number:1 ; pages:0 |
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10.1046/j.1471-4159.1996.66010026.x |
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| 520 | |a Abstract: Gangliosides are synthesized by sequential catalytic reaction of multiple glycosyltransferases. GM2/GD2 synthase and GD3 synthase are key enzymes for ganglioside synthesis, because their relative activities regulate the main profiles of ganglioside expression. Mouse GD3 synthase (EC 2.4.99.8) cDNA was cloned by eukaryotic expression cloning, and its mRNA expression as well as that of GM2/GD2 synthase gene during the development of the mouse CNS was analyzed by using northern blotting, reverse transcription-polymerase chain reaction, and in situ hybridization. When brain tissue was analyzed as a whole mass, a typical pattern corresponding to the reported findings obtained by biochemical analyses was observed, i.e., high expression of GD3 synthase gene in the early stage and gradual increase of GM2/GD2 synthase gene expression in the late stage of the development. However, the results of in situ hybridization of these two genes revealed that the expression kinetics of these two genes were heterogeneous among various sites in the brain under development. These findings suggest that various expression patterns of the two genes reflect differences in the course of the development of individual sites, and also different ganglioside components are required in individual portions of the brain for development and maintenance of the function. | ||
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10.1046/j.1471-4159.1996.66010026.x doi (DE-627)NLEJ240230744 DE-627 ger DE-627 rakwb Heterogeneity in the Expression Pattern of Two Ganglioside Synthase Genes During Mouse Brain Development Oxford, UK Blackwell Science Ltd 1996 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract: Gangliosides are synthesized by sequential catalytic reaction of multiple glycosyltransferases. GM2/GD2 synthase and GD3 synthase are key enzymes for ganglioside synthesis, because their relative activities regulate the main profiles of ganglioside expression. Mouse GD3 synthase (EC 2.4.99.8) cDNA was cloned by eukaryotic expression cloning, and its mRNA expression as well as that of GM2/GD2 synthase gene during the development of the mouse CNS was analyzed by using northern blotting, reverse transcription-polymerase chain reaction, and in situ hybridization. When brain tissue was analyzed as a whole mass, a typical pattern corresponding to the reported findings obtained by biochemical analyses was observed, i.e., high expression of GD3 synthase gene in the early stage and gradual increase of GM2/GD2 synthase gene expression in the late stage of the development. However, the results of in situ hybridization of these two genes revealed that the expression kinetics of these two genes were heterogeneous among various sites in the brain under development. These findings suggest that various expression patterns of the two genes reflect differences in the course of the development of individual sites, and also different ganglioside components are required in individual portions of the brain for development and maintenance of the function. 2002 Blackwell Publishing Journal Backfiles 1879-2005 |2002|||||||||| Glycosyltransferase Yamamoto, Akihito oth Haraguchi, Masashi oth Yamashiro, Shuji oth Fukumoto, Satoshi oth Furukawa, Keiko oth Takamiya, Kogo oth Atsuta, Mitsuru oth Shiku, Hiroshi oth Furukawa, Koichi oth In Journal of neurochemistry Oxford : Wiley-Blackwell, 1956 66(1996), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927584 (DE-600)2020528-4 1471-4159 nnns volume:66 year:1996 number:1 pages:0 http://dx.doi.org/10.1046/j.1471-4159.1996.66010026.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 66 1996 1 0 |
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10.1046/j.1471-4159.1996.66010026.x doi (DE-627)NLEJ240230744 DE-627 ger DE-627 rakwb Heterogeneity in the Expression Pattern of Two Ganglioside Synthase Genes During Mouse Brain Development Oxford, UK Blackwell Science Ltd 1996 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract: Gangliosides are synthesized by sequential catalytic reaction of multiple glycosyltransferases. GM2/GD2 synthase and GD3 synthase are key enzymes for ganglioside synthesis, because their relative activities regulate the main profiles of ganglioside expression. Mouse GD3 synthase (EC 2.4.99.8) cDNA was cloned by eukaryotic expression cloning, and its mRNA expression as well as that of GM2/GD2 synthase gene during the development of the mouse CNS was analyzed by using northern blotting, reverse transcription-polymerase chain reaction, and in situ hybridization. When brain tissue was analyzed as a whole mass, a typical pattern corresponding to the reported findings obtained by biochemical analyses was observed, i.e., high expression of GD3 synthase gene in the early stage and gradual increase of GM2/GD2 synthase gene expression in the late stage of the development. However, the results of in situ hybridization of these two genes revealed that the expression kinetics of these two genes were heterogeneous among various sites in the brain under development. These findings suggest that various expression patterns of the two genes reflect differences in the course of the development of individual sites, and also different ganglioside components are required in individual portions of the brain for development and maintenance of the function. 2002 Blackwell Publishing Journal Backfiles 1879-2005 |2002|||||||||| Glycosyltransferase Yamamoto, Akihito oth Haraguchi, Masashi oth Yamashiro, Shuji oth Fukumoto, Satoshi oth Furukawa, Keiko oth Takamiya, Kogo oth Atsuta, Mitsuru oth Shiku, Hiroshi oth Furukawa, Koichi oth In Journal of neurochemistry Oxford : Wiley-Blackwell, 1956 66(1996), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927584 (DE-600)2020528-4 1471-4159 nnns volume:66 year:1996 number:1 pages:0 http://dx.doi.org/10.1046/j.1471-4159.1996.66010026.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 66 1996 1 0 |
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10.1046/j.1471-4159.1996.66010026.x doi (DE-627)NLEJ240230744 DE-627 ger DE-627 rakwb Heterogeneity in the Expression Pattern of Two Ganglioside Synthase Genes During Mouse Brain Development Oxford, UK Blackwell Science Ltd 1996 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract: Gangliosides are synthesized by sequential catalytic reaction of multiple glycosyltransferases. GM2/GD2 synthase and GD3 synthase are key enzymes for ganglioside synthesis, because their relative activities regulate the main profiles of ganglioside expression. Mouse GD3 synthase (EC 2.4.99.8) cDNA was cloned by eukaryotic expression cloning, and its mRNA expression as well as that of GM2/GD2 synthase gene during the development of the mouse CNS was analyzed by using northern blotting, reverse transcription-polymerase chain reaction, and in situ hybridization. When brain tissue was analyzed as a whole mass, a typical pattern corresponding to the reported findings obtained by biochemical analyses was observed, i.e., high expression of GD3 synthase gene in the early stage and gradual increase of GM2/GD2 synthase gene expression in the late stage of the development. However, the results of in situ hybridization of these two genes revealed that the expression kinetics of these two genes were heterogeneous among various sites in the brain under development. These findings suggest that various expression patterns of the two genes reflect differences in the course of the development of individual sites, and also different ganglioside components are required in individual portions of the brain for development and maintenance of the function. 2002 Blackwell Publishing Journal Backfiles 1879-2005 |2002|||||||||| Glycosyltransferase Yamamoto, Akihito oth Haraguchi, Masashi oth Yamashiro, Shuji oth Fukumoto, Satoshi oth Furukawa, Keiko oth Takamiya, Kogo oth Atsuta, Mitsuru oth Shiku, Hiroshi oth Furukawa, Koichi oth In Journal of neurochemistry Oxford : Wiley-Blackwell, 1956 66(1996), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927584 (DE-600)2020528-4 1471-4159 nnns volume:66 year:1996 number:1 pages:0 http://dx.doi.org/10.1046/j.1471-4159.1996.66010026.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 66 1996 1 0 |
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10.1046/j.1471-4159.1996.66010026.x doi (DE-627)NLEJ240230744 DE-627 ger DE-627 rakwb Heterogeneity in the Expression Pattern of Two Ganglioside Synthase Genes During Mouse Brain Development Oxford, UK Blackwell Science Ltd 1996 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract: Gangliosides are synthesized by sequential catalytic reaction of multiple glycosyltransferases. GM2/GD2 synthase and GD3 synthase are key enzymes for ganglioside synthesis, because their relative activities regulate the main profiles of ganglioside expression. Mouse GD3 synthase (EC 2.4.99.8) cDNA was cloned by eukaryotic expression cloning, and its mRNA expression as well as that of GM2/GD2 synthase gene during the development of the mouse CNS was analyzed by using northern blotting, reverse transcription-polymerase chain reaction, and in situ hybridization. When brain tissue was analyzed as a whole mass, a typical pattern corresponding to the reported findings obtained by biochemical analyses was observed, i.e., high expression of GD3 synthase gene in the early stage and gradual increase of GM2/GD2 synthase gene expression in the late stage of the development. However, the results of in situ hybridization of these two genes revealed that the expression kinetics of these two genes were heterogeneous among various sites in the brain under development. These findings suggest that various expression patterns of the two genes reflect differences in the course of the development of individual sites, and also different ganglioside components are required in individual portions of the brain for development and maintenance of the function. 2002 Blackwell Publishing Journal Backfiles 1879-2005 |2002|||||||||| Glycosyltransferase Yamamoto, Akihito oth Haraguchi, Masashi oth Yamashiro, Shuji oth Fukumoto, Satoshi oth Furukawa, Keiko oth Takamiya, Kogo oth Atsuta, Mitsuru oth Shiku, Hiroshi oth Furukawa, Koichi oth In Journal of neurochemistry Oxford : Wiley-Blackwell, 1956 66(1996), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927584 (DE-600)2020528-4 1471-4159 nnns volume:66 year:1996 number:1 pages:0 http://dx.doi.org/10.1046/j.1471-4159.1996.66010026.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 66 1996 1 0 |
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10.1046/j.1471-4159.1996.66010026.x doi (DE-627)NLEJ240230744 DE-627 ger DE-627 rakwb Heterogeneity in the Expression Pattern of Two Ganglioside Synthase Genes During Mouse Brain Development Oxford, UK Blackwell Science Ltd 1996 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract: Gangliosides are synthesized by sequential catalytic reaction of multiple glycosyltransferases. GM2/GD2 synthase and GD3 synthase are key enzymes for ganglioside synthesis, because their relative activities regulate the main profiles of ganglioside expression. Mouse GD3 synthase (EC 2.4.99.8) cDNA was cloned by eukaryotic expression cloning, and its mRNA expression as well as that of GM2/GD2 synthase gene during the development of the mouse CNS was analyzed by using northern blotting, reverse transcription-polymerase chain reaction, and in situ hybridization. When brain tissue was analyzed as a whole mass, a typical pattern corresponding to the reported findings obtained by biochemical analyses was observed, i.e., high expression of GD3 synthase gene in the early stage and gradual increase of GM2/GD2 synthase gene expression in the late stage of the development. However, the results of in situ hybridization of these two genes revealed that the expression kinetics of these two genes were heterogeneous among various sites in the brain under development. These findings suggest that various expression patterns of the two genes reflect differences in the course of the development of individual sites, and also different ganglioside components are required in individual portions of the brain for development and maintenance of the function. 2002 Blackwell Publishing Journal Backfiles 1879-2005 |2002|||||||||| Glycosyltransferase Yamamoto, Akihito oth Haraguchi, Masashi oth Yamashiro, Shuji oth Fukumoto, Satoshi oth Furukawa, Keiko oth Takamiya, Kogo oth Atsuta, Mitsuru oth Shiku, Hiroshi oth Furukawa, Koichi oth In Journal of neurochemistry Oxford : Wiley-Blackwell, 1956 66(1996), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927584 (DE-600)2020528-4 1471-4159 nnns volume:66 year:1996 number:1 pages:0 http://dx.doi.org/10.1046/j.1471-4159.1996.66010026.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 66 1996 1 0 |
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heterogeneity in the expression pattern of two ganglioside synthase genes during mouse brain development |
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Heterogeneity in the Expression Pattern of Two Ganglioside Synthase Genes During Mouse Brain Development |
| abstract |
Abstract: Gangliosides are synthesized by sequential catalytic reaction of multiple glycosyltransferases. GM2/GD2 synthase and GD3 synthase are key enzymes for ganglioside synthesis, because their relative activities regulate the main profiles of ganglioside expression. Mouse GD3 synthase (EC 2.4.99.8) cDNA was cloned by eukaryotic expression cloning, and its mRNA expression as well as that of GM2/GD2 synthase gene during the development of the mouse CNS was analyzed by using northern blotting, reverse transcription-polymerase chain reaction, and in situ hybridization. When brain tissue was analyzed as a whole mass, a typical pattern corresponding to the reported findings obtained by biochemical analyses was observed, i.e., high expression of GD3 synthase gene in the early stage and gradual increase of GM2/GD2 synthase gene expression in the late stage of the development. However, the results of in situ hybridization of these two genes revealed that the expression kinetics of these two genes were heterogeneous among various sites in the brain under development. These findings suggest that various expression patterns of the two genes reflect differences in the course of the development of individual sites, and also different ganglioside components are required in individual portions of the brain for development and maintenance of the function. |
| abstractGer |
Abstract: Gangliosides are synthesized by sequential catalytic reaction of multiple glycosyltransferases. GM2/GD2 synthase and GD3 synthase are key enzymes for ganglioside synthesis, because their relative activities regulate the main profiles of ganglioside expression. Mouse GD3 synthase (EC 2.4.99.8) cDNA was cloned by eukaryotic expression cloning, and its mRNA expression as well as that of GM2/GD2 synthase gene during the development of the mouse CNS was analyzed by using northern blotting, reverse transcription-polymerase chain reaction, and in situ hybridization. When brain tissue was analyzed as a whole mass, a typical pattern corresponding to the reported findings obtained by biochemical analyses was observed, i.e., high expression of GD3 synthase gene in the early stage and gradual increase of GM2/GD2 synthase gene expression in the late stage of the development. However, the results of in situ hybridization of these two genes revealed that the expression kinetics of these two genes were heterogeneous among various sites in the brain under development. These findings suggest that various expression patterns of the two genes reflect differences in the course of the development of individual sites, and also different ganglioside components are required in individual portions of the brain for development and maintenance of the function. |
| abstract_unstemmed |
Abstract: Gangliosides are synthesized by sequential catalytic reaction of multiple glycosyltransferases. GM2/GD2 synthase and GD3 synthase are key enzymes for ganglioside synthesis, because their relative activities regulate the main profiles of ganglioside expression. Mouse GD3 synthase (EC 2.4.99.8) cDNA was cloned by eukaryotic expression cloning, and its mRNA expression as well as that of GM2/GD2 synthase gene during the development of the mouse CNS was analyzed by using northern blotting, reverse transcription-polymerase chain reaction, and in situ hybridization. When brain tissue was analyzed as a whole mass, a typical pattern corresponding to the reported findings obtained by biochemical analyses was observed, i.e., high expression of GD3 synthase gene in the early stage and gradual increase of GM2/GD2 synthase gene expression in the late stage of the development. However, the results of in situ hybridization of these two genes revealed that the expression kinetics of these two genes were heterogeneous among various sites in the brain under development. These findings suggest that various expression patterns of the two genes reflect differences in the course of the development of individual sites, and also different ganglioside components are required in individual portions of the brain for development and maintenance of the function. |
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Heterogeneity in the Expression Pattern of Two Ganglioside Synthase Genes During Mouse Brain Development |
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Yamamoto, Akihito Haraguchi, Masashi Yamashiro, Shuji Fukumoto, Satoshi Furukawa, Keiko Takamiya, Kogo Atsuta, Mitsuru Shiku, Hiroshi Furukawa, Koichi |
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Yamamoto, Akihito Haraguchi, Masashi Yamashiro, Shuji Fukumoto, Satoshi Furukawa, Keiko Takamiya, Kogo Atsuta, Mitsuru Shiku, Hiroshi Furukawa, Koichi |
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