ON THE MECHANISM OF OUABAIN INHIBITION OF SYNAPTOSOME PROTEIN SYNTHESIS
Abstract— Ouabain (200μm) inhibited incorporation of radiolabelled leucine or glycine into the protein of neonatal synaptosome fractions but had minimal effect on preparations from adult rats. Leucine uptake into synaptosomes was rapid but not influenced by 200μm-ouabain in contrast to ouabain inhib...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Verity, M. Anthony [verfasserIn] Brown, W. Jann [verfasserIn] Cheung, Marshall K. [verfasserIn] |
|---|
| Format: |
E-Artikel |
|---|
| Erschienen: |
Oxford, UK: Blackwell Publishing Ltd ; 1979 |
|---|
| Umfang: |
Online-Ressource |
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| Reproduktion: |
2006 ; Blackwell Publishing Journal Backfiles 1879-2005 |
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| Übergeordnetes Werk: |
In: Journal of neurochemistry - Oxford : Wiley-Blackwell, 1956, 32(1979), 4, Seite 0 |
| Übergeordnetes Werk: |
volume:32 ; year:1979 ; number:4 ; pages:0 |
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| DOI / URN: |
10.1111/j.1471-4159.1979.tb11057.x |
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| 520 | |a Abstract— Ouabain (200μm) inhibited incorporation of radiolabelled leucine or glycine into the protein of neonatal synaptosome fractions but had minimal effect on preparations from adult rats. Leucine uptake into synaptosomes was rapid but not influenced by 200μm-ouabain in contrast to ouabain inhibition of [14C]glycine and [14C]γ-aminobutyric acid uptake. Ouabain blocked the Na+ -dependent (stimulated) component of synaptosome fraction protein synthesis in the presence of 25mm-K+. Ouabain inhibition was not alleviated by addition of ADP or ATP. 100μm-atractylate failed to influence [3H]leucine uptake or incorporation. Synergistic inhibition by ouabain was observed with the cycloheximide-sensitive component of protein synthesis and the chloramphenicol sensitive phase. Increasing the medium Ca2+ concentration stimulated protein synthesis and this stimulated component was inhibited by ouabain. Ouabain inhibition was associated with decreasing intraterminal K+ concentration and [K]i was linearly related to the protein synthesis rate in control and ouabain treated preparations. | ||
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10.1111/j.1471-4159.1979.tb11057.x doi (DE-627)NLEJ240331702 DE-627 ger DE-627 rakwb Verity, M. Anthony verfasserin aut ON THE MECHANISM OF OUABAIN INHIBITION OF SYNAPTOSOME PROTEIN SYNTHESIS Oxford, UK Blackwell Publishing Ltd 1979 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract— Ouabain (200μm) inhibited incorporation of radiolabelled leucine or glycine into the protein of neonatal synaptosome fractions but had minimal effect on preparations from adult rats. Leucine uptake into synaptosomes was rapid but not influenced by 200μm-ouabain in contrast to ouabain inhibition of [14C]glycine and [14C]γ-aminobutyric acid uptake. Ouabain blocked the Na+ -dependent (stimulated) component of synaptosome fraction protein synthesis in the presence of 25mm-K+. Ouabain inhibition was not alleviated by addition of ADP or ATP. 100μm-atractylate failed to influence [3H]leucine uptake or incorporation. Synergistic inhibition by ouabain was observed with the cycloheximide-sensitive component of protein synthesis and the chloramphenicol sensitive phase. Increasing the medium Ca2+ concentration stimulated protein synthesis and this stimulated component was inhibited by ouabain. Ouabain inhibition was associated with decreasing intraterminal K+ concentration and [K]i was linearly related to the protein synthesis rate in control and ouabain treated preparations. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Brown, W. Jann verfasserin aut Cheung, Marshall K. verfasserin aut In Journal of neurochemistry Oxford : Wiley-Blackwell, 1956 32(1979), 4, Seite 0 Online-Ressource (DE-627)NLEJ243927584 (DE-600)2020528-4 1471-4159 nnns volume:32 year:1979 number:4 pages:0 http://dx.doi.org/10.1111/j.1471-4159.1979.tb11057.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 32 1979 4 0 |
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10.1111/j.1471-4159.1979.tb11057.x doi (DE-627)NLEJ240331702 DE-627 ger DE-627 rakwb Verity, M. Anthony verfasserin aut ON THE MECHANISM OF OUABAIN INHIBITION OF SYNAPTOSOME PROTEIN SYNTHESIS Oxford, UK Blackwell Publishing Ltd 1979 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract— Ouabain (200μm) inhibited incorporation of radiolabelled leucine or glycine into the protein of neonatal synaptosome fractions but had minimal effect on preparations from adult rats. Leucine uptake into synaptosomes was rapid but not influenced by 200μm-ouabain in contrast to ouabain inhibition of [14C]glycine and [14C]γ-aminobutyric acid uptake. Ouabain blocked the Na+ -dependent (stimulated) component of synaptosome fraction protein synthesis in the presence of 25mm-K+. Ouabain inhibition was not alleviated by addition of ADP or ATP. 100μm-atractylate failed to influence [3H]leucine uptake or incorporation. Synergistic inhibition by ouabain was observed with the cycloheximide-sensitive component of protein synthesis and the chloramphenicol sensitive phase. Increasing the medium Ca2+ concentration stimulated protein synthesis and this stimulated component was inhibited by ouabain. Ouabain inhibition was associated with decreasing intraterminal K+ concentration and [K]i was linearly related to the protein synthesis rate in control and ouabain treated preparations. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Brown, W. Jann verfasserin aut Cheung, Marshall K. verfasserin aut In Journal of neurochemistry Oxford : Wiley-Blackwell, 1956 32(1979), 4, Seite 0 Online-Ressource (DE-627)NLEJ243927584 (DE-600)2020528-4 1471-4159 nnns volume:32 year:1979 number:4 pages:0 http://dx.doi.org/10.1111/j.1471-4159.1979.tb11057.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 32 1979 4 0 |
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10.1111/j.1471-4159.1979.tb11057.x doi (DE-627)NLEJ240331702 DE-627 ger DE-627 rakwb Verity, M. Anthony verfasserin aut ON THE MECHANISM OF OUABAIN INHIBITION OF SYNAPTOSOME PROTEIN SYNTHESIS Oxford, UK Blackwell Publishing Ltd 1979 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract— Ouabain (200μm) inhibited incorporation of radiolabelled leucine or glycine into the protein of neonatal synaptosome fractions but had minimal effect on preparations from adult rats. Leucine uptake into synaptosomes was rapid but not influenced by 200μm-ouabain in contrast to ouabain inhibition of [14C]glycine and [14C]γ-aminobutyric acid uptake. Ouabain blocked the Na+ -dependent (stimulated) component of synaptosome fraction protein synthesis in the presence of 25mm-K+. Ouabain inhibition was not alleviated by addition of ADP or ATP. 100μm-atractylate failed to influence [3H]leucine uptake or incorporation. Synergistic inhibition by ouabain was observed with the cycloheximide-sensitive component of protein synthesis and the chloramphenicol sensitive phase. Increasing the medium Ca2+ concentration stimulated protein synthesis and this stimulated component was inhibited by ouabain. Ouabain inhibition was associated with decreasing intraterminal K+ concentration and [K]i was linearly related to the protein synthesis rate in control and ouabain treated preparations. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Brown, W. Jann verfasserin aut Cheung, Marshall K. verfasserin aut In Journal of neurochemistry Oxford : Wiley-Blackwell, 1956 32(1979), 4, Seite 0 Online-Ressource (DE-627)NLEJ243927584 (DE-600)2020528-4 1471-4159 nnns volume:32 year:1979 number:4 pages:0 http://dx.doi.org/10.1111/j.1471-4159.1979.tb11057.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 32 1979 4 0 |
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10.1111/j.1471-4159.1979.tb11057.x doi (DE-627)NLEJ240331702 DE-627 ger DE-627 rakwb Verity, M. Anthony verfasserin aut ON THE MECHANISM OF OUABAIN INHIBITION OF SYNAPTOSOME PROTEIN SYNTHESIS Oxford, UK Blackwell Publishing Ltd 1979 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract— Ouabain (200μm) inhibited incorporation of radiolabelled leucine or glycine into the protein of neonatal synaptosome fractions but had minimal effect on preparations from adult rats. Leucine uptake into synaptosomes was rapid but not influenced by 200μm-ouabain in contrast to ouabain inhibition of [14C]glycine and [14C]γ-aminobutyric acid uptake. Ouabain blocked the Na+ -dependent (stimulated) component of synaptosome fraction protein synthesis in the presence of 25mm-K+. Ouabain inhibition was not alleviated by addition of ADP or ATP. 100μm-atractylate failed to influence [3H]leucine uptake or incorporation. Synergistic inhibition by ouabain was observed with the cycloheximide-sensitive component of protein synthesis and the chloramphenicol sensitive phase. Increasing the medium Ca2+ concentration stimulated protein synthesis and this stimulated component was inhibited by ouabain. Ouabain inhibition was associated with decreasing intraterminal K+ concentration and [K]i was linearly related to the protein synthesis rate in control and ouabain treated preparations. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Brown, W. Jann verfasserin aut Cheung, Marshall K. verfasserin aut In Journal of neurochemistry Oxford : Wiley-Blackwell, 1956 32(1979), 4, Seite 0 Online-Ressource (DE-627)NLEJ243927584 (DE-600)2020528-4 1471-4159 nnns volume:32 year:1979 number:4 pages:0 http://dx.doi.org/10.1111/j.1471-4159.1979.tb11057.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 32 1979 4 0 |
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10.1111/j.1471-4159.1979.tb11057.x doi (DE-627)NLEJ240331702 DE-627 ger DE-627 rakwb Verity, M. Anthony verfasserin aut ON THE MECHANISM OF OUABAIN INHIBITION OF SYNAPTOSOME PROTEIN SYNTHESIS Oxford, UK Blackwell Publishing Ltd 1979 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract— Ouabain (200μm) inhibited incorporation of radiolabelled leucine or glycine into the protein of neonatal synaptosome fractions but had minimal effect on preparations from adult rats. Leucine uptake into synaptosomes was rapid but not influenced by 200μm-ouabain in contrast to ouabain inhibition of [14C]glycine and [14C]γ-aminobutyric acid uptake. Ouabain blocked the Na+ -dependent (stimulated) component of synaptosome fraction protein synthesis in the presence of 25mm-K+. Ouabain inhibition was not alleviated by addition of ADP or ATP. 100μm-atractylate failed to influence [3H]leucine uptake or incorporation. Synergistic inhibition by ouabain was observed with the cycloheximide-sensitive component of protein synthesis and the chloramphenicol sensitive phase. Increasing the medium Ca2+ concentration stimulated protein synthesis and this stimulated component was inhibited by ouabain. Ouabain inhibition was associated with decreasing intraterminal K+ concentration and [K]i was linearly related to the protein synthesis rate in control and ouabain treated preparations. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Brown, W. Jann verfasserin aut Cheung, Marshall K. verfasserin aut In Journal of neurochemistry Oxford : Wiley-Blackwell, 1956 32(1979), 4, Seite 0 Online-Ressource (DE-627)NLEJ243927584 (DE-600)2020528-4 1471-4159 nnns volume:32 year:1979 number:4 pages:0 http://dx.doi.org/10.1111/j.1471-4159.1979.tb11057.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 32 1979 4 0 |
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Abstract— Ouabain (200μm) inhibited incorporation of radiolabelled leucine or glycine into the protein of neonatal synaptosome fractions but had minimal effect on preparations from adult rats. Leucine uptake into synaptosomes was rapid but not influenced by 200μm-ouabain in contrast to ouabain inhibition of [14C]glycine and [14C]γ-aminobutyric acid uptake. Ouabain blocked the Na+ -dependent (stimulated) component of synaptosome fraction protein synthesis in the presence of 25mm-K+. Ouabain inhibition was not alleviated by addition of ADP or ATP. 100μm-atractylate failed to influence [3H]leucine uptake or incorporation. Synergistic inhibition by ouabain was observed with the cycloheximide-sensitive component of protein synthesis and the chloramphenicol sensitive phase. Increasing the medium Ca2+ concentration stimulated protein synthesis and this stimulated component was inhibited by ouabain. Ouabain inhibition was associated with decreasing intraterminal K+ concentration and [K]i was linearly related to the protein synthesis rate in control and ouabain treated preparations. |
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Abstract— Ouabain (200μm) inhibited incorporation of radiolabelled leucine or glycine into the protein of neonatal synaptosome fractions but had minimal effect on preparations from adult rats. Leucine uptake into synaptosomes was rapid but not influenced by 200μm-ouabain in contrast to ouabain inhibition of [14C]glycine and [14C]γ-aminobutyric acid uptake. Ouabain blocked the Na+ -dependent (stimulated) component of synaptosome fraction protein synthesis in the presence of 25mm-K+. Ouabain inhibition was not alleviated by addition of ADP or ATP. 100μm-atractylate failed to influence [3H]leucine uptake or incorporation. Synergistic inhibition by ouabain was observed with the cycloheximide-sensitive component of protein synthesis and the chloramphenicol sensitive phase. Increasing the medium Ca2+ concentration stimulated protein synthesis and this stimulated component was inhibited by ouabain. Ouabain inhibition was associated with decreasing intraterminal K+ concentration and [K]i was linearly related to the protein synthesis rate in control and ouabain treated preparations. |
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Abstract— Ouabain (200μm) inhibited incorporation of radiolabelled leucine or glycine into the protein of neonatal synaptosome fractions but had minimal effect on preparations from adult rats. Leucine uptake into synaptosomes was rapid but not influenced by 200μm-ouabain in contrast to ouabain inhibition of [14C]glycine and [14C]γ-aminobutyric acid uptake. Ouabain blocked the Na+ -dependent (stimulated) component of synaptosome fraction protein synthesis in the presence of 25mm-K+. Ouabain inhibition was not alleviated by addition of ADP or ATP. 100μm-atractylate failed to influence [3H]leucine uptake or incorporation. Synergistic inhibition by ouabain was observed with the cycloheximide-sensitive component of protein synthesis and the chloramphenicol sensitive phase. Increasing the medium Ca2+ concentration stimulated protein synthesis and this stimulated component was inhibited by ouabain. Ouabain inhibition was associated with decreasing intraterminal K+ concentration and [K]i was linearly related to the protein synthesis rate in control and ouabain treated preparations. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ240331702</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210707110342.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">120426s1979 xx |||||o 00| ||und c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1111/j.1471-4159.1979.tb11057.x</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ240331702</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Verity, M. Anthony</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">ON THE MECHANISM OF OUABAIN INHIBITION OF SYNAPTOSOME PROTEIN SYNTHESIS</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Oxford, UK</subfield><subfield code="b">Blackwell Publishing Ltd</subfield><subfield code="c">1979</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract— Ouabain (200μm) inhibited incorporation of radiolabelled leucine or glycine into the protein of neonatal synaptosome fractions but had minimal effect on preparations from adult rats. Leucine uptake into synaptosomes was rapid but not influenced by 200μm-ouabain in contrast to ouabain inhibition of [14C]glycine and [14C]γ-aminobutyric acid uptake. Ouabain blocked the Na+ -dependent (stimulated) component of synaptosome fraction protein synthesis in the presence of 25mm-K+. Ouabain inhibition was not alleviated by addition of ADP or ATP. 100μm-atractylate failed to influence [3H]leucine uptake or incorporation. Synergistic inhibition by ouabain was observed with the cycloheximide-sensitive component of protein synthesis and the chloramphenicol sensitive phase. Increasing the medium Ca2+ concentration stimulated protein synthesis and this stimulated component was inhibited by ouabain. Ouabain inhibition was associated with decreasing intraterminal K+ concentration and [K]i was linearly related to the protein synthesis rate in control and ouabain treated preparations.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="d">2006</subfield><subfield code="f">Blackwell Publishing Journal Backfiles 1879-2005</subfield><subfield code="7">|2006||||||||||</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Brown, W. Jann</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Cheung, Marshall K.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Journal of neurochemistry</subfield><subfield code="d">Oxford : Wiley-Blackwell, 1956</subfield><subfield code="g">32(1979), 4, Seite 0</subfield><subfield code="h">Online-Ressource</subfield><subfield code="w">(DE-627)NLEJ243927584</subfield><subfield code="w">(DE-600)2020528-4</subfield><subfield code="x">1471-4159</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:32</subfield><subfield code="g">year:1979</subfield><subfield code="g">number:4</subfield><subfield code="g">pages:0</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1111/j.1471-4159.1979.tb11057.x</subfield><subfield code="q">text/html</subfield><subfield code="x">Verlag</subfield><subfield code="z">Deutschlandweit zugänglich</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-DJB</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">32</subfield><subfield code="j">1979</subfield><subfield code="e">4</subfield><subfield code="h">0</subfield></datafield></record></collection>
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