m-OCTOPAMINE, p-OCTOPAMINE AND PHENYLETHANOLAMINE IN RAT BRAIN: A SENSITIVE, SPECIFIC ASSAY AND THE EFFECTS OF SOME DRUGS
Abstract— An enzyme radiochemical assay for p-octopamine, m-octopamine (norphenylephrine) and phenylethanolamine based on the N-methylation of these amines by the enzyme phenylethanolamine N-methyl transferase (S-adenosyl-l-methionine: phenylethanolamine N-methyl transferase (EC 2.1.1.28) has been d...
Ausführliche Beschreibung
Autor*in: |
Danielson, T. J. [verfasserIn] Boulton, A. A. [verfasserIn] Robertson, H. A. [verfasserIn] |
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E-Artikel |
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Erschienen: |
Oxford, UK: Blackwell Publishing Ltd ; 1977 |
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Online-Ressource |
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Reproduktion: |
2006 ; Blackwell Publishing Journal Backfiles 1879-2005 |
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Übergeordnetes Werk: |
In: Journal of neurochemistry - Oxford : Wiley-Blackwell, 1956, 29(1977), 6, Seite 0 |
Übergeordnetes Werk: |
volume:29 ; year:1977 ; number:6 ; pages:0 |
Links: |
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DOI / URN: |
10.1111/j.1471-4159.1977.tb06519.x |
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10.1111/j.1471-4159.1977.tb06519.x doi (DE-627)NLEJ240338979 DE-627 ger DE-627 rakwb Danielson, T. J. verfasserin aut m-OCTOPAMINE, p-OCTOPAMINE AND PHENYLETHANOLAMINE IN RAT BRAIN: A SENSITIVE, SPECIFIC ASSAY AND THE EFFECTS OF SOME DRUGS Oxford, UK Blackwell Publishing Ltd 1977 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract— An enzyme radiochemical assay for p-octopamine, m-octopamine (norphenylephrine) and phenylethanolamine based on the N-methylation of these amines by the enzyme phenylethanolamine N-methyl transferase (S-adenosyl-l-methionine: phenylethanolamine N-methyl transferase (EC 2.1.1.28) has been developed. [3H]Methyl-S-adenosyl-l-methionine was used as methyl donor. The reaction products are converted to their dansyl derivatives and separated by TLC in three different solvent systems prior to liquid scintillation counting. The method exhibits a sensitivity of less than 10 pg for each amine and is suitable for the measurement of endogenous p-octopamine levels in mammalian brain. The highest levels of p-octopamine were found in the hypothalamus (3.4 ng/g) but despite the sensitivity of the assay, neither phenylethanolamine nor m-octopamine could be detected. After MAO inhibition, however, both of these amines were found to be present. p-Octopamine was increased substantially in all brain regions following the administration of an MAO inhibitor, whereas pretreatment with reserpine produced a significant decrease in the hypothalamus. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Boulton, A. A. verfasserin aut Robertson, H. A. verfasserin aut In Journal of neurochemistry Oxford : Wiley-Blackwell, 1956 29(1977), 6, Seite 0 Online-Ressource (DE-627)NLEJ243927584 (DE-600)2020528-4 1471-4159 nnns volume:29 year:1977 number:6 pages:0 http://dx.doi.org/10.1111/j.1471-4159.1977.tb06519.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 29 1977 6 0 |
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10.1111/j.1471-4159.1977.tb06519.x doi (DE-627)NLEJ240338979 DE-627 ger DE-627 rakwb Danielson, T. J. verfasserin aut m-OCTOPAMINE, p-OCTOPAMINE AND PHENYLETHANOLAMINE IN RAT BRAIN: A SENSITIVE, SPECIFIC ASSAY AND THE EFFECTS OF SOME DRUGS Oxford, UK Blackwell Publishing Ltd 1977 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract— An enzyme radiochemical assay for p-octopamine, m-octopamine (norphenylephrine) and phenylethanolamine based on the N-methylation of these amines by the enzyme phenylethanolamine N-methyl transferase (S-adenosyl-l-methionine: phenylethanolamine N-methyl transferase (EC 2.1.1.28) has been developed. [3H]Methyl-S-adenosyl-l-methionine was used as methyl donor. The reaction products are converted to their dansyl derivatives and separated by TLC in three different solvent systems prior to liquid scintillation counting. The method exhibits a sensitivity of less than 10 pg for each amine and is suitable for the measurement of endogenous p-octopamine levels in mammalian brain. The highest levels of p-octopamine were found in the hypothalamus (3.4 ng/g) but despite the sensitivity of the assay, neither phenylethanolamine nor m-octopamine could be detected. After MAO inhibition, however, both of these amines were found to be present. p-Octopamine was increased substantially in all brain regions following the administration of an MAO inhibitor, whereas pretreatment with reserpine produced a significant decrease in the hypothalamus. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Boulton, A. A. verfasserin aut Robertson, H. A. verfasserin aut In Journal of neurochemistry Oxford : Wiley-Blackwell, 1956 29(1977), 6, Seite 0 Online-Ressource (DE-627)NLEJ243927584 (DE-600)2020528-4 1471-4159 nnns volume:29 year:1977 number:6 pages:0 http://dx.doi.org/10.1111/j.1471-4159.1977.tb06519.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 29 1977 6 0 |
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10.1111/j.1471-4159.1977.tb06519.x doi (DE-627)NLEJ240338979 DE-627 ger DE-627 rakwb Danielson, T. J. verfasserin aut m-OCTOPAMINE, p-OCTOPAMINE AND PHENYLETHANOLAMINE IN RAT BRAIN: A SENSITIVE, SPECIFIC ASSAY AND THE EFFECTS OF SOME DRUGS Oxford, UK Blackwell Publishing Ltd 1977 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract— An enzyme radiochemical assay for p-octopamine, m-octopamine (norphenylephrine) and phenylethanolamine based on the N-methylation of these amines by the enzyme phenylethanolamine N-methyl transferase (S-adenosyl-l-methionine: phenylethanolamine N-methyl transferase (EC 2.1.1.28) has been developed. [3H]Methyl-S-adenosyl-l-methionine was used as methyl donor. The reaction products are converted to their dansyl derivatives and separated by TLC in three different solvent systems prior to liquid scintillation counting. The method exhibits a sensitivity of less than 10 pg for each amine and is suitable for the measurement of endogenous p-octopamine levels in mammalian brain. The highest levels of p-octopamine were found in the hypothalamus (3.4 ng/g) but despite the sensitivity of the assay, neither phenylethanolamine nor m-octopamine could be detected. After MAO inhibition, however, both of these amines were found to be present. p-Octopamine was increased substantially in all brain regions following the administration of an MAO inhibitor, whereas pretreatment with reserpine produced a significant decrease in the hypothalamus. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Boulton, A. A. verfasserin aut Robertson, H. A. verfasserin aut In Journal of neurochemistry Oxford : Wiley-Blackwell, 1956 29(1977), 6, Seite 0 Online-Ressource (DE-627)NLEJ243927584 (DE-600)2020528-4 1471-4159 nnns volume:29 year:1977 number:6 pages:0 http://dx.doi.org/10.1111/j.1471-4159.1977.tb06519.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 29 1977 6 0 |
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10.1111/j.1471-4159.1977.tb06519.x doi (DE-627)NLEJ240338979 DE-627 ger DE-627 rakwb Danielson, T. J. verfasserin aut m-OCTOPAMINE, p-OCTOPAMINE AND PHENYLETHANOLAMINE IN RAT BRAIN: A SENSITIVE, SPECIFIC ASSAY AND THE EFFECTS OF SOME DRUGS Oxford, UK Blackwell Publishing Ltd 1977 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract— An enzyme radiochemical assay for p-octopamine, m-octopamine (norphenylephrine) and phenylethanolamine based on the N-methylation of these amines by the enzyme phenylethanolamine N-methyl transferase (S-adenosyl-l-methionine: phenylethanolamine N-methyl transferase (EC 2.1.1.28) has been developed. [3H]Methyl-S-adenosyl-l-methionine was used as methyl donor. The reaction products are converted to their dansyl derivatives and separated by TLC in three different solvent systems prior to liquid scintillation counting. The method exhibits a sensitivity of less than 10 pg for each amine and is suitable for the measurement of endogenous p-octopamine levels in mammalian brain. The highest levels of p-octopamine were found in the hypothalamus (3.4 ng/g) but despite the sensitivity of the assay, neither phenylethanolamine nor m-octopamine could be detected. After MAO inhibition, however, both of these amines were found to be present. p-Octopamine was increased substantially in all brain regions following the administration of an MAO inhibitor, whereas pretreatment with reserpine produced a significant decrease in the hypothalamus. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Boulton, A. A. verfasserin aut Robertson, H. A. verfasserin aut In Journal of neurochemistry Oxford : Wiley-Blackwell, 1956 29(1977), 6, Seite 0 Online-Ressource (DE-627)NLEJ243927584 (DE-600)2020528-4 1471-4159 nnns volume:29 year:1977 number:6 pages:0 http://dx.doi.org/10.1111/j.1471-4159.1977.tb06519.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 29 1977 6 0 |
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m-OCTOPAMINE, p-OCTOPAMINE AND PHENYLETHANOLAMINE IN RAT BRAIN: A SENSITIVE, SPECIFIC ASSAY AND THE EFFECTS OF SOME DRUGS |
abstract |
Abstract— An enzyme radiochemical assay for p-octopamine, m-octopamine (norphenylephrine) and phenylethanolamine based on the N-methylation of these amines by the enzyme phenylethanolamine N-methyl transferase (S-adenosyl-l-methionine: phenylethanolamine N-methyl transferase (EC 2.1.1.28) has been developed. [3H]Methyl-S-adenosyl-l-methionine was used as methyl donor. The reaction products are converted to their dansyl derivatives and separated by TLC in three different solvent systems prior to liquid scintillation counting. The method exhibits a sensitivity of less than 10 pg for each amine and is suitable for the measurement of endogenous p-octopamine levels in mammalian brain. The highest levels of p-octopamine were found in the hypothalamus (3.4 ng/g) but despite the sensitivity of the assay, neither phenylethanolamine nor m-octopamine could be detected. After MAO inhibition, however, both of these amines were found to be present. p-Octopamine was increased substantially in all brain regions following the administration of an MAO inhibitor, whereas pretreatment with reserpine produced a significant decrease in the hypothalamus. |
abstractGer |
Abstract— An enzyme radiochemical assay for p-octopamine, m-octopamine (norphenylephrine) and phenylethanolamine based on the N-methylation of these amines by the enzyme phenylethanolamine N-methyl transferase (S-adenosyl-l-methionine: phenylethanolamine N-methyl transferase (EC 2.1.1.28) has been developed. [3H]Methyl-S-adenosyl-l-methionine was used as methyl donor. The reaction products are converted to their dansyl derivatives and separated by TLC in three different solvent systems prior to liquid scintillation counting. The method exhibits a sensitivity of less than 10 pg for each amine and is suitable for the measurement of endogenous p-octopamine levels in mammalian brain. The highest levels of p-octopamine were found in the hypothalamus (3.4 ng/g) but despite the sensitivity of the assay, neither phenylethanolamine nor m-octopamine could be detected. After MAO inhibition, however, both of these amines were found to be present. p-Octopamine was increased substantially in all brain regions following the administration of an MAO inhibitor, whereas pretreatment with reserpine produced a significant decrease in the hypothalamus. |
abstract_unstemmed |
Abstract— An enzyme radiochemical assay for p-octopamine, m-octopamine (norphenylephrine) and phenylethanolamine based on the N-methylation of these amines by the enzyme phenylethanolamine N-methyl transferase (S-adenosyl-l-methionine: phenylethanolamine N-methyl transferase (EC 2.1.1.28) has been developed. [3H]Methyl-S-adenosyl-l-methionine was used as methyl donor. The reaction products are converted to their dansyl derivatives and separated by TLC in three different solvent systems prior to liquid scintillation counting. The method exhibits a sensitivity of less than 10 pg for each amine and is suitable for the measurement of endogenous p-octopamine levels in mammalian brain. The highest levels of p-octopamine were found in the hypothalamus (3.4 ng/g) but despite the sensitivity of the assay, neither phenylethanolamine nor m-octopamine could be detected. After MAO inhibition, however, both of these amines were found to be present. p-Octopamine was increased substantially in all brain regions following the administration of an MAO inhibitor, whereas pretreatment with reserpine produced a significant decrease in the hypothalamus. |
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title_short |
m-OCTOPAMINE, p-OCTOPAMINE AND PHENYLETHANOLAMINE IN RAT BRAIN: A SENSITIVE, SPECIFIC ASSAY AND THE EFFECTS OF SOME DRUGS |
url |
http://dx.doi.org/10.1111/j.1471-4159.1977.tb06519.x |
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Boulton, A. A. Robertson, H. A. |
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10.1111/j.1471-4159.1977.tb06519.x |
up_date |
2024-07-06T09:45:08.987Z |
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