Activation of rainbow trout macrophages
Rainbow trout peritoneal macrophages were stimulated in vitro using Concanavalin A (Con A) and in vivo using formalin-killed Aeromonas salmonicida in Freund's incomplete adjuvant (FIA). Whether these cells had been activated was determined by the measurements of oxygen anions (NBT reduction), H...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Chung, S. [verfasserIn] Secombes, C. J. [verfasserIn] |
|---|
| Format: |
E-Artikel |
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| Erschienen: |
Oxford, UK: Blackwell Publishing Ltd ; 1987 |
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| Umfang: |
Online-Ressource |
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| Reproduktion: |
2006 ; Blackwell Publishing Journal Backfiles 1879-2005 |
|---|---|
| Übergeordnetes Werk: |
In: Journal of fish biology - Oxford [u.a.] : Wiley-Blackwell, 1969, 31(1987), Seite 0 |
| Übergeordnetes Werk: |
volume:31 ; year:1987 ; pages:0 |
| Links: |
|---|
| DOI / URN: |
10.1111/j.1095-8649.1987.tb05292.x |
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NLEJ240609190 |
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| 520 | |a Rainbow trout peritoneal macrophages were stimulated in vitro using Concanavalin A (Con A) and in vivo using formalin-killed Aeromonas salmonicida in Freund's incomplete adjuvant (FIA). Whether these cells had been activated was determined by the measurements of oxygen anions (NBT reduction), H2O2 production (oxidation of phenol red), RNA synthesis (3H-uridine incorporation), acid phosphatase activity and bactericidal activity. In vitro-stimulated macrophages showed an increased NBT reduction and 3H-uridine incorporation over a range of Con A concentrations, compared with untreated control macrophages, but no detectable increases in H2O2 production or bactericidal activity were observed. On the other hand, in vivo-stimulated peritoneal cells showed increases in all the assays compared with FIA-elicited control cells, and were considered to have been activated. | ||
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10.1111/j.1095-8649.1987.tb05292.x doi (DE-627)NLEJ240609190 DE-627 ger DE-627 rakwb Chung, S. verfasserin aut Activation of rainbow trout macrophages Oxford, UK Blackwell Publishing Ltd 1987 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Rainbow trout peritoneal macrophages were stimulated in vitro using Concanavalin A (Con A) and in vivo using formalin-killed Aeromonas salmonicida in Freund's incomplete adjuvant (FIA). Whether these cells had been activated was determined by the measurements of oxygen anions (NBT reduction), H2O2 production (oxidation of phenol red), RNA synthesis (3H-uridine incorporation), acid phosphatase activity and bactericidal activity. In vitro-stimulated macrophages showed an increased NBT reduction and 3H-uridine incorporation over a range of Con A concentrations, compared with untreated control macrophages, but no detectable increases in H2O2 production or bactericidal activity were observed. On the other hand, in vivo-stimulated peritoneal cells showed increases in all the assays compared with FIA-elicited control cells, and were considered to have been activated. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Secombes, C. J. verfasserin aut In Journal of fish biology Oxford [u.a.] : Wiley-Blackwell, 1969 31(1987), Seite 0 Online-Ressource (DE-627)NLEJ24392741X (DE-600)1471958-7 1095-8649 nnns volume:31 year:1987 pages:0 http://dx.doi.org/10.1111/j.1095-8649.1987.tb05292.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 31 1987 0 |
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10.1111/j.1095-8649.1987.tb05292.x doi (DE-627)NLEJ240609190 DE-627 ger DE-627 rakwb Chung, S. verfasserin aut Activation of rainbow trout macrophages Oxford, UK Blackwell Publishing Ltd 1987 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Rainbow trout peritoneal macrophages were stimulated in vitro using Concanavalin A (Con A) and in vivo using formalin-killed Aeromonas salmonicida in Freund's incomplete adjuvant (FIA). Whether these cells had been activated was determined by the measurements of oxygen anions (NBT reduction), H2O2 production (oxidation of phenol red), RNA synthesis (3H-uridine incorporation), acid phosphatase activity and bactericidal activity. In vitro-stimulated macrophages showed an increased NBT reduction and 3H-uridine incorporation over a range of Con A concentrations, compared with untreated control macrophages, but no detectable increases in H2O2 production or bactericidal activity were observed. On the other hand, in vivo-stimulated peritoneal cells showed increases in all the assays compared with FIA-elicited control cells, and were considered to have been activated. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Secombes, C. J. verfasserin aut In Journal of fish biology Oxford [u.a.] : Wiley-Blackwell, 1969 31(1987), Seite 0 Online-Ressource (DE-627)NLEJ24392741X (DE-600)1471958-7 1095-8649 nnns volume:31 year:1987 pages:0 http://dx.doi.org/10.1111/j.1095-8649.1987.tb05292.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 31 1987 0 |
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10.1111/j.1095-8649.1987.tb05292.x doi (DE-627)NLEJ240609190 DE-627 ger DE-627 rakwb Chung, S. verfasserin aut Activation of rainbow trout macrophages Oxford, UK Blackwell Publishing Ltd 1987 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Rainbow trout peritoneal macrophages were stimulated in vitro using Concanavalin A (Con A) and in vivo using formalin-killed Aeromonas salmonicida in Freund's incomplete adjuvant (FIA). Whether these cells had been activated was determined by the measurements of oxygen anions (NBT reduction), H2O2 production (oxidation of phenol red), RNA synthesis (3H-uridine incorporation), acid phosphatase activity and bactericidal activity. In vitro-stimulated macrophages showed an increased NBT reduction and 3H-uridine incorporation over a range of Con A concentrations, compared with untreated control macrophages, but no detectable increases in H2O2 production or bactericidal activity were observed. On the other hand, in vivo-stimulated peritoneal cells showed increases in all the assays compared with FIA-elicited control cells, and were considered to have been activated. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Secombes, C. J. verfasserin aut In Journal of fish biology Oxford [u.a.] : Wiley-Blackwell, 1969 31(1987), Seite 0 Online-Ressource (DE-627)NLEJ24392741X (DE-600)1471958-7 1095-8649 nnns volume:31 year:1987 pages:0 http://dx.doi.org/10.1111/j.1095-8649.1987.tb05292.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 31 1987 0 |
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10.1111/j.1095-8649.1987.tb05292.x doi (DE-627)NLEJ240609190 DE-627 ger DE-627 rakwb Chung, S. verfasserin aut Activation of rainbow trout macrophages Oxford, UK Blackwell Publishing Ltd 1987 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Rainbow trout peritoneal macrophages were stimulated in vitro using Concanavalin A (Con A) and in vivo using formalin-killed Aeromonas salmonicida in Freund's incomplete adjuvant (FIA). Whether these cells had been activated was determined by the measurements of oxygen anions (NBT reduction), H2O2 production (oxidation of phenol red), RNA synthesis (3H-uridine incorporation), acid phosphatase activity and bactericidal activity. In vitro-stimulated macrophages showed an increased NBT reduction and 3H-uridine incorporation over a range of Con A concentrations, compared with untreated control macrophages, but no detectable increases in H2O2 production or bactericidal activity were observed. On the other hand, in vivo-stimulated peritoneal cells showed increases in all the assays compared with FIA-elicited control cells, and were considered to have been activated. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Secombes, C. J. verfasserin aut In Journal of fish biology Oxford [u.a.] : Wiley-Blackwell, 1969 31(1987), Seite 0 Online-Ressource (DE-627)NLEJ24392741X (DE-600)1471958-7 1095-8649 nnns volume:31 year:1987 pages:0 http://dx.doi.org/10.1111/j.1095-8649.1987.tb05292.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 31 1987 0 |
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10.1111/j.1095-8649.1987.tb05292.x doi (DE-627)NLEJ240609190 DE-627 ger DE-627 rakwb Chung, S. verfasserin aut Activation of rainbow trout macrophages Oxford, UK Blackwell Publishing Ltd 1987 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Rainbow trout peritoneal macrophages were stimulated in vitro using Concanavalin A (Con A) and in vivo using formalin-killed Aeromonas salmonicida in Freund's incomplete adjuvant (FIA). Whether these cells had been activated was determined by the measurements of oxygen anions (NBT reduction), H2O2 production (oxidation of phenol red), RNA synthesis (3H-uridine incorporation), acid phosphatase activity and bactericidal activity. In vitro-stimulated macrophages showed an increased NBT reduction and 3H-uridine incorporation over a range of Con A concentrations, compared with untreated control macrophages, but no detectable increases in H2O2 production or bactericidal activity were observed. On the other hand, in vivo-stimulated peritoneal cells showed increases in all the assays compared with FIA-elicited control cells, and were considered to have been activated. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Secombes, C. J. verfasserin aut In Journal of fish biology Oxford [u.a.] : Wiley-Blackwell, 1969 31(1987), Seite 0 Online-Ressource (DE-627)NLEJ24392741X (DE-600)1471958-7 1095-8649 nnns volume:31 year:1987 pages:0 http://dx.doi.org/10.1111/j.1095-8649.1987.tb05292.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 31 1987 0 |
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| abstract |
Rainbow trout peritoneal macrophages were stimulated in vitro using Concanavalin A (Con A) and in vivo using formalin-killed Aeromonas salmonicida in Freund's incomplete adjuvant (FIA). Whether these cells had been activated was determined by the measurements of oxygen anions (NBT reduction), H2O2 production (oxidation of phenol red), RNA synthesis (3H-uridine incorporation), acid phosphatase activity and bactericidal activity. In vitro-stimulated macrophages showed an increased NBT reduction and 3H-uridine incorporation over a range of Con A concentrations, compared with untreated control macrophages, but no detectable increases in H2O2 production or bactericidal activity were observed. On the other hand, in vivo-stimulated peritoneal cells showed increases in all the assays compared with FIA-elicited control cells, and were considered to have been activated. |
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Rainbow trout peritoneal macrophages were stimulated in vitro using Concanavalin A (Con A) and in vivo using formalin-killed Aeromonas salmonicida in Freund's incomplete adjuvant (FIA). Whether these cells had been activated was determined by the measurements of oxygen anions (NBT reduction), H2O2 production (oxidation of phenol red), RNA synthesis (3H-uridine incorporation), acid phosphatase activity and bactericidal activity. In vitro-stimulated macrophages showed an increased NBT reduction and 3H-uridine incorporation over a range of Con A concentrations, compared with untreated control macrophages, but no detectable increases in H2O2 production or bactericidal activity were observed. On the other hand, in vivo-stimulated peritoneal cells showed increases in all the assays compared with FIA-elicited control cells, and were considered to have been activated. |
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Rainbow trout peritoneal macrophages were stimulated in vitro using Concanavalin A (Con A) and in vivo using formalin-killed Aeromonas salmonicida in Freund's incomplete adjuvant (FIA). Whether these cells had been activated was determined by the measurements of oxygen anions (NBT reduction), H2O2 production (oxidation of phenol red), RNA synthesis (3H-uridine incorporation), acid phosphatase activity and bactericidal activity. In vitro-stimulated macrophages showed an increased NBT reduction and 3H-uridine incorporation over a range of Con A concentrations, compared with untreated control macrophages, but no detectable increases in H2O2 production or bactericidal activity were observed. On the other hand, in vivo-stimulated peritoneal cells showed increases in all the assays compared with FIA-elicited control cells, and were considered to have been activated. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ240609190</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210707114506.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">120426s1987 xx |||||o 00| ||und c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1111/j.1095-8649.1987.tb05292.x</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ240609190</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Chung, S.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Activation of rainbow trout macrophages</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Oxford, UK</subfield><subfield code="b">Blackwell Publishing Ltd</subfield><subfield code="c">1987</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Rainbow trout peritoneal macrophages were stimulated in vitro using Concanavalin A (Con A) and in vivo using formalin-killed Aeromonas salmonicida in Freund's incomplete adjuvant (FIA). Whether these cells had been activated was determined by the measurements of oxygen anions (NBT reduction), H2O2 production (oxidation of phenol red), RNA synthesis (3H-uridine incorporation), acid phosphatase activity and bactericidal activity. In vitro-stimulated macrophages showed an increased NBT reduction and 3H-uridine incorporation over a range of Con A concentrations, compared with untreated control macrophages, but no detectable increases in H2O2 production or bactericidal activity were observed. On the other hand, in vivo-stimulated peritoneal cells showed increases in all the assays compared with FIA-elicited control cells, and were considered to have been activated.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="d">2006</subfield><subfield code="f">Blackwell Publishing Journal Backfiles 1879-2005</subfield><subfield code="7">|2006||||||||||</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Secombes, C. J.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Journal of fish biology</subfield><subfield code="d">Oxford [u.a.] : Wiley-Blackwell, 1969</subfield><subfield code="g">31(1987), Seite 0</subfield><subfield code="h">Online-Ressource</subfield><subfield code="w">(DE-627)NLEJ24392741X</subfield><subfield code="w">(DE-600)1471958-7</subfield><subfield code="x">1095-8649</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:31</subfield><subfield code="g">year:1987</subfield><subfield code="g">pages:0</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1111/j.1095-8649.1987.tb05292.x</subfield><subfield code="q">text/html</subfield><subfield code="x">Verlag</subfield><subfield code="z">Deutschlandweit zugänglich</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-DJB</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">31</subfield><subfield code="j">1987</subfield><subfield code="h">0</subfield></datafield></record></collection>
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7.399579 |