A Temperature-Sensitive Mutation Affecting Synthesis of Outer Arm Dyneins in Tetrahymena thermophila
. We have characterized a novel, temperature-sensitive mutation affecting motility in Tetrahymena thermophila. Mutants grew and divided normally at the restrictive temperature (38°C), but became nonmotile. Scanning electron microscopic analysis indicated that nonmotile mutants contained the normal n...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
ATTWELL, GWILYM J. [verfasserIn] BRICKER, CONNIE S. [verfasserIn] SCHWANDT, ANITA [verfasserIn] |
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| Format: |
E-Artikel |
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| Erschienen: |
Oxford, UK: Blackwell Publishing Ltd ; 1992 |
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| Schlagwörter: |
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| Umfang: |
Online-Ressource |
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| Reproduktion: |
2007 ; Blackwell Publishing Journal Backfiles 1879-2005 |
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| Übergeordnetes Werk: |
In: The journal of eukaryotic microbiology - Oxford [u.a.] : Wiley-Blackwell, 1954, 39(1992), 2, Seite 0 |
| Übergeordnetes Werk: |
volume:39 ; year:1992 ; number:2 ; pages:0 |
| Links: |
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| DOI / URN: |
10.1111/j.1550-7408.1992.tb01312.x |
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NLEJ240637429 |
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| 520 | |a . We have characterized a novel, temperature-sensitive mutation affecting motility in Tetrahymena thermophila. Mutants grew and divided normally at the restrictive temperature (38°C), but became nonmotile. Scanning electron microscopic analysis indicated that nonmotile mutants contained the normal number of cilia and that the cilia were of normal length. Transmission electron microscopic analysis indicated that axonemes isolated from nonmotile mutants lacked outer dynein arms, so the mutation was named oad I (outer arm defficient). Motile mutants shifted to 38° C under conditions that prevent cell growth and division (starvation) remained motile suggesting that once assembled into axonemes at the permissive temperature (28° C) the outer arm dyneins remain functional at 38° C. Starved, deciliated mutants regenerated a full complement of functional cilia at 38° C, indicating that the mechanism that incorporates the outer arm dynein into developing axonemes is not affected by the oad I mutation. Starved, nonmotile mutants regained motility when shifted back to 28° C, but not when incubated with cycloheximide. We interpret these results to rule out the hypothesis that the oad I mutation affects the site on the microtubules to which the outer arm dyneins bind. Axonemes isolated from mutants grown for one generation at 38° C had a mean of 6.0 outer arm dyneins, and axonemes isolated from mutants grown for two generations at 38° C had a mean of 3.2 outer arm dyneins. Taken together, these results indicate that the oad I mutation affects the synthesis of outer arm dyneins in Tetrahymena. | ||
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10.1111/j.1550-7408.1992.tb01312.x doi (DE-627)NLEJ240637429 DE-627 ger DE-627 rakwb ATTWELL, GWILYM J. verfasserin aut A Temperature-Sensitive Mutation Affecting Synthesis of Outer Arm Dyneins in Tetrahymena thermophila Oxford, UK Blackwell Publishing Ltd 1992 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier . We have characterized a novel, temperature-sensitive mutation affecting motility in Tetrahymena thermophila. Mutants grew and divided normally at the restrictive temperature (38°C), but became nonmotile. Scanning electron microscopic analysis indicated that nonmotile mutants contained the normal number of cilia and that the cilia were of normal length. Transmission electron microscopic analysis indicated that axonemes isolated from nonmotile mutants lacked outer dynein arms, so the mutation was named oad I (outer arm defficient). Motile mutants shifted to 38° C under conditions that prevent cell growth and division (starvation) remained motile suggesting that once assembled into axonemes at the permissive temperature (28° C) the outer arm dyneins remain functional at 38° C. Starved, deciliated mutants regenerated a full complement of functional cilia at 38° C, indicating that the mechanism that incorporates the outer arm dynein into developing axonemes is not affected by the oad I mutation. Starved, nonmotile mutants regained motility when shifted back to 28° C, but not when incubated with cycloheximide. We interpret these results to rule out the hypothesis that the oad I mutation affects the site on the microtubules to which the outer arm dyneins bind. Axonemes isolated from mutants grown for one generation at 38° C had a mean of 6.0 outer arm dyneins, and axonemes isolated from mutants grown for two generations at 38° C had a mean of 3.2 outer arm dyneins. Taken together, these results indicate that the oad I mutation affects the synthesis of outer arm dyneins in Tetrahymena. 2007 Blackwell Publishing Journal Backfiles 1879-2005 |2007|||||||||| Cilia BRICKER, CONNIE S. verfasserin aut SCHWANDT, ANITA verfasserin aut GOROVSKY, MARTIN A. oth PENNOCK, DAVID G. oth In The journal of eukaryotic microbiology Oxford [u.a.] : Wiley-Blackwell, 1954 39(1992), 2, Seite 0 Online-Ressource (DE-627)NLEJ243927622 (DE-600)2126326-7 1550-7408 nnns volume:39 year:1992 number:2 pages:0 http://dx.doi.org/10.1111/j.1550-7408.1992.tb01312.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 39 1992 2 0 |
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10.1111/j.1550-7408.1992.tb01312.x doi (DE-627)NLEJ240637429 DE-627 ger DE-627 rakwb ATTWELL, GWILYM J. verfasserin aut A Temperature-Sensitive Mutation Affecting Synthesis of Outer Arm Dyneins in Tetrahymena thermophila Oxford, UK Blackwell Publishing Ltd 1992 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier . We have characterized a novel, temperature-sensitive mutation affecting motility in Tetrahymena thermophila. Mutants grew and divided normally at the restrictive temperature (38°C), but became nonmotile. Scanning electron microscopic analysis indicated that nonmotile mutants contained the normal number of cilia and that the cilia were of normal length. Transmission electron microscopic analysis indicated that axonemes isolated from nonmotile mutants lacked outer dynein arms, so the mutation was named oad I (outer arm defficient). Motile mutants shifted to 38° C under conditions that prevent cell growth and division (starvation) remained motile suggesting that once assembled into axonemes at the permissive temperature (28° C) the outer arm dyneins remain functional at 38° C. Starved, deciliated mutants regenerated a full complement of functional cilia at 38° C, indicating that the mechanism that incorporates the outer arm dynein into developing axonemes is not affected by the oad I mutation. Starved, nonmotile mutants regained motility when shifted back to 28° C, but not when incubated with cycloheximide. We interpret these results to rule out the hypothesis that the oad I mutation affects the site on the microtubules to which the outer arm dyneins bind. Axonemes isolated from mutants grown for one generation at 38° C had a mean of 6.0 outer arm dyneins, and axonemes isolated from mutants grown for two generations at 38° C had a mean of 3.2 outer arm dyneins. Taken together, these results indicate that the oad I mutation affects the synthesis of outer arm dyneins in Tetrahymena. 2007 Blackwell Publishing Journal Backfiles 1879-2005 |2007|||||||||| Cilia BRICKER, CONNIE S. verfasserin aut SCHWANDT, ANITA verfasserin aut GOROVSKY, MARTIN A. oth PENNOCK, DAVID G. oth In The journal of eukaryotic microbiology Oxford [u.a.] : Wiley-Blackwell, 1954 39(1992), 2, Seite 0 Online-Ressource (DE-627)NLEJ243927622 (DE-600)2126326-7 1550-7408 nnns volume:39 year:1992 number:2 pages:0 http://dx.doi.org/10.1111/j.1550-7408.1992.tb01312.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 39 1992 2 0 |
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10.1111/j.1550-7408.1992.tb01312.x doi (DE-627)NLEJ240637429 DE-627 ger DE-627 rakwb ATTWELL, GWILYM J. verfasserin aut A Temperature-Sensitive Mutation Affecting Synthesis of Outer Arm Dyneins in Tetrahymena thermophila Oxford, UK Blackwell Publishing Ltd 1992 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier . We have characterized a novel, temperature-sensitive mutation affecting motility in Tetrahymena thermophila. Mutants grew and divided normally at the restrictive temperature (38°C), but became nonmotile. Scanning electron microscopic analysis indicated that nonmotile mutants contained the normal number of cilia and that the cilia were of normal length. Transmission electron microscopic analysis indicated that axonemes isolated from nonmotile mutants lacked outer dynein arms, so the mutation was named oad I (outer arm defficient). Motile mutants shifted to 38° C under conditions that prevent cell growth and division (starvation) remained motile suggesting that once assembled into axonemes at the permissive temperature (28° C) the outer arm dyneins remain functional at 38° C. Starved, deciliated mutants regenerated a full complement of functional cilia at 38° C, indicating that the mechanism that incorporates the outer arm dynein into developing axonemes is not affected by the oad I mutation. Starved, nonmotile mutants regained motility when shifted back to 28° C, but not when incubated with cycloheximide. We interpret these results to rule out the hypothesis that the oad I mutation affects the site on the microtubules to which the outer arm dyneins bind. Axonemes isolated from mutants grown for one generation at 38° C had a mean of 6.0 outer arm dyneins, and axonemes isolated from mutants grown for two generations at 38° C had a mean of 3.2 outer arm dyneins. Taken together, these results indicate that the oad I mutation affects the synthesis of outer arm dyneins in Tetrahymena. 2007 Blackwell Publishing Journal Backfiles 1879-2005 |2007|||||||||| Cilia BRICKER, CONNIE S. verfasserin aut SCHWANDT, ANITA verfasserin aut GOROVSKY, MARTIN A. oth PENNOCK, DAVID G. oth In The journal of eukaryotic microbiology Oxford [u.a.] : Wiley-Blackwell, 1954 39(1992), 2, Seite 0 Online-Ressource (DE-627)NLEJ243927622 (DE-600)2126326-7 1550-7408 nnns volume:39 year:1992 number:2 pages:0 http://dx.doi.org/10.1111/j.1550-7408.1992.tb01312.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 39 1992 2 0 |
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10.1111/j.1550-7408.1992.tb01312.x doi (DE-627)NLEJ240637429 DE-627 ger DE-627 rakwb ATTWELL, GWILYM J. verfasserin aut A Temperature-Sensitive Mutation Affecting Synthesis of Outer Arm Dyneins in Tetrahymena thermophila Oxford, UK Blackwell Publishing Ltd 1992 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier . We have characterized a novel, temperature-sensitive mutation affecting motility in Tetrahymena thermophila. Mutants grew and divided normally at the restrictive temperature (38°C), but became nonmotile. Scanning electron microscopic analysis indicated that nonmotile mutants contained the normal number of cilia and that the cilia were of normal length. Transmission electron microscopic analysis indicated that axonemes isolated from nonmotile mutants lacked outer dynein arms, so the mutation was named oad I (outer arm defficient). Motile mutants shifted to 38° C under conditions that prevent cell growth and division (starvation) remained motile suggesting that once assembled into axonemes at the permissive temperature (28° C) the outer arm dyneins remain functional at 38° C. Starved, deciliated mutants regenerated a full complement of functional cilia at 38° C, indicating that the mechanism that incorporates the outer arm dynein into developing axonemes is not affected by the oad I mutation. Starved, nonmotile mutants regained motility when shifted back to 28° C, but not when incubated with cycloheximide. We interpret these results to rule out the hypothesis that the oad I mutation affects the site on the microtubules to which the outer arm dyneins bind. Axonemes isolated from mutants grown for one generation at 38° C had a mean of 6.0 outer arm dyneins, and axonemes isolated from mutants grown for two generations at 38° C had a mean of 3.2 outer arm dyneins. Taken together, these results indicate that the oad I mutation affects the synthesis of outer arm dyneins in Tetrahymena. 2007 Blackwell Publishing Journal Backfiles 1879-2005 |2007|||||||||| Cilia BRICKER, CONNIE S. verfasserin aut SCHWANDT, ANITA verfasserin aut GOROVSKY, MARTIN A. oth PENNOCK, DAVID G. oth In The journal of eukaryotic microbiology Oxford [u.a.] : Wiley-Blackwell, 1954 39(1992), 2, Seite 0 Online-Ressource (DE-627)NLEJ243927622 (DE-600)2126326-7 1550-7408 nnns volume:39 year:1992 number:2 pages:0 http://dx.doi.org/10.1111/j.1550-7408.1992.tb01312.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 39 1992 2 0 |
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10.1111/j.1550-7408.1992.tb01312.x doi (DE-627)NLEJ240637429 DE-627 ger DE-627 rakwb ATTWELL, GWILYM J. verfasserin aut A Temperature-Sensitive Mutation Affecting Synthesis of Outer Arm Dyneins in Tetrahymena thermophila Oxford, UK Blackwell Publishing Ltd 1992 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier . We have characterized a novel, temperature-sensitive mutation affecting motility in Tetrahymena thermophila. Mutants grew and divided normally at the restrictive temperature (38°C), but became nonmotile. Scanning electron microscopic analysis indicated that nonmotile mutants contained the normal number of cilia and that the cilia were of normal length. Transmission electron microscopic analysis indicated that axonemes isolated from nonmotile mutants lacked outer dynein arms, so the mutation was named oad I (outer arm defficient). Motile mutants shifted to 38° C under conditions that prevent cell growth and division (starvation) remained motile suggesting that once assembled into axonemes at the permissive temperature (28° C) the outer arm dyneins remain functional at 38° C. Starved, deciliated mutants regenerated a full complement of functional cilia at 38° C, indicating that the mechanism that incorporates the outer arm dynein into developing axonemes is not affected by the oad I mutation. Starved, nonmotile mutants regained motility when shifted back to 28° C, but not when incubated with cycloheximide. We interpret these results to rule out the hypothesis that the oad I mutation affects the site on the microtubules to which the outer arm dyneins bind. Axonemes isolated from mutants grown for one generation at 38° C had a mean of 6.0 outer arm dyneins, and axonemes isolated from mutants grown for two generations at 38° C had a mean of 3.2 outer arm dyneins. Taken together, these results indicate that the oad I mutation affects the synthesis of outer arm dyneins in Tetrahymena. 2007 Blackwell Publishing Journal Backfiles 1879-2005 |2007|||||||||| Cilia BRICKER, CONNIE S. verfasserin aut SCHWANDT, ANITA verfasserin aut GOROVSKY, MARTIN A. oth PENNOCK, DAVID G. oth In The journal of eukaryotic microbiology Oxford [u.a.] : Wiley-Blackwell, 1954 39(1992), 2, Seite 0 Online-Ressource (DE-627)NLEJ243927622 (DE-600)2126326-7 1550-7408 nnns volume:39 year:1992 number:2 pages:0 http://dx.doi.org/10.1111/j.1550-7408.1992.tb01312.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 39 1992 2 0 |
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ATTWELL, GWILYM J. |
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10.1111/j.1550-7408.1992.tb01312.x |
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verfasserin |
| title_sort |
a temperature-sensitive mutation affecting synthesis of outer arm dyneins in tetrahymena thermophila |
| title_auth |
A Temperature-Sensitive Mutation Affecting Synthesis of Outer Arm Dyneins in Tetrahymena thermophila |
| abstract |
. We have characterized a novel, temperature-sensitive mutation affecting motility in Tetrahymena thermophila. Mutants grew and divided normally at the restrictive temperature (38°C), but became nonmotile. Scanning electron microscopic analysis indicated that nonmotile mutants contained the normal number of cilia and that the cilia were of normal length. Transmission electron microscopic analysis indicated that axonemes isolated from nonmotile mutants lacked outer dynein arms, so the mutation was named oad I (outer arm defficient). Motile mutants shifted to 38° C under conditions that prevent cell growth and division (starvation) remained motile suggesting that once assembled into axonemes at the permissive temperature (28° C) the outer arm dyneins remain functional at 38° C. Starved, deciliated mutants regenerated a full complement of functional cilia at 38° C, indicating that the mechanism that incorporates the outer arm dynein into developing axonemes is not affected by the oad I mutation. Starved, nonmotile mutants regained motility when shifted back to 28° C, but not when incubated with cycloheximide. We interpret these results to rule out the hypothesis that the oad I mutation affects the site on the microtubules to which the outer arm dyneins bind. Axonemes isolated from mutants grown for one generation at 38° C had a mean of 6.0 outer arm dyneins, and axonemes isolated from mutants grown for two generations at 38° C had a mean of 3.2 outer arm dyneins. Taken together, these results indicate that the oad I mutation affects the synthesis of outer arm dyneins in Tetrahymena. |
| abstractGer |
. We have characterized a novel, temperature-sensitive mutation affecting motility in Tetrahymena thermophila. Mutants grew and divided normally at the restrictive temperature (38°C), but became nonmotile. Scanning electron microscopic analysis indicated that nonmotile mutants contained the normal number of cilia and that the cilia were of normal length. Transmission electron microscopic analysis indicated that axonemes isolated from nonmotile mutants lacked outer dynein arms, so the mutation was named oad I (outer arm defficient). Motile mutants shifted to 38° C under conditions that prevent cell growth and division (starvation) remained motile suggesting that once assembled into axonemes at the permissive temperature (28° C) the outer arm dyneins remain functional at 38° C. Starved, deciliated mutants regenerated a full complement of functional cilia at 38° C, indicating that the mechanism that incorporates the outer arm dynein into developing axonemes is not affected by the oad I mutation. Starved, nonmotile mutants regained motility when shifted back to 28° C, but not when incubated with cycloheximide. We interpret these results to rule out the hypothesis that the oad I mutation affects the site on the microtubules to which the outer arm dyneins bind. Axonemes isolated from mutants grown for one generation at 38° C had a mean of 6.0 outer arm dyneins, and axonemes isolated from mutants grown for two generations at 38° C had a mean of 3.2 outer arm dyneins. Taken together, these results indicate that the oad I mutation affects the synthesis of outer arm dyneins in Tetrahymena. |
| abstract_unstemmed |
. We have characterized a novel, temperature-sensitive mutation affecting motility in Tetrahymena thermophila. Mutants grew and divided normally at the restrictive temperature (38°C), but became nonmotile. Scanning electron microscopic analysis indicated that nonmotile mutants contained the normal number of cilia and that the cilia were of normal length. Transmission electron microscopic analysis indicated that axonemes isolated from nonmotile mutants lacked outer dynein arms, so the mutation was named oad I (outer arm defficient). Motile mutants shifted to 38° C under conditions that prevent cell growth and division (starvation) remained motile suggesting that once assembled into axonemes at the permissive temperature (28° C) the outer arm dyneins remain functional at 38° C. Starved, deciliated mutants regenerated a full complement of functional cilia at 38° C, indicating that the mechanism that incorporates the outer arm dynein into developing axonemes is not affected by the oad I mutation. Starved, nonmotile mutants regained motility when shifted back to 28° C, but not when incubated with cycloheximide. We interpret these results to rule out the hypothesis that the oad I mutation affects the site on the microtubules to which the outer arm dyneins bind. Axonemes isolated from mutants grown for one generation at 38° C had a mean of 6.0 outer arm dyneins, and axonemes isolated from mutants grown for two generations at 38° C had a mean of 3.2 outer arm dyneins. Taken together, these results indicate that the oad I mutation affects the synthesis of outer arm dyneins in Tetrahymena. |
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A Temperature-Sensitive Mutation Affecting Synthesis of Outer Arm Dyneins in Tetrahymena thermophila |
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BRICKER, CONNIE S. SCHWANDT, ANITA GOROVSKY, MARTIN A. PENNOCK, DAVID G. |
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