Measurement of CO2 and HCO3− fluxes in cyanobacteria and microalgae during steady-state photosynthesis
A mass spectrometric procedure is described which allows the simultaneous estimation of both CO2 and HCO−3 fluxes associated with cyanobacteria and green algae during steady-stale photosynthesis. This technique utilizes the chemical disequilibrium which exists between CO2 and HCO−3 during photosynth...
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| Autor*in: |
Badger, Murray R. [verfasserIn] Palmqvist, Kristin [verfasserIn] Yu, Jian-Wei [verfasserIn] |
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| Format: |
E-Artikel |
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| Erschienen: |
Oxford, UK: Blackwell Publishing Ltd ; 1994 |
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| Schlagwörter: |
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| Umfang: |
Online-Ressource |
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| Reproduktion: |
2006 ; Blackwell Publishing Journal Backfiles 1879-2005 |
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| Übergeordnetes Werk: |
In: Physiologia plantarum - Oxford [u.a.] : Wiley-Blackwell, 1948, 90(1994), 3, Seite 0 |
| Übergeordnetes Werk: |
volume:90 ; year:1994 ; number:3 ; pages:0 |
| Links: |
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| DOI / URN: |
10.1111/j.1399-3054.1994.tb08811.x |
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NLEJ240943112 |
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| 520 | |a A mass spectrometric procedure is described which allows the simultaneous estimation of both CO2 and HCO−3 fluxes associated with cyanobacteria and green algae during steady-stale photosynthesis. This technique utilizes the chemical disequilibrium which exists between CO2 and HCO−3 during photosynthesis in cell suspensions which lack carbonic anhydrase activity. The kinetic equations which are derived for flux determinations are based on models of photosynthesis in both cyanobacteria and green algae which seem most reasonable given our present level of understanding, together with direct measurement of [CO2] estimation of [HCO−3] and application of the kinetic rate constants for the interconversion of CO2 and HCO−3 From measurements made in the light, net uptake of both CO2 and HCO−3 can be readily determined. In addition, analysis of the dark phase immediately following light-off provides the possibility of also determining the CO2 evolution whích ís occurring during photosynthesis, and thus also the gross CO2 uptake rates in the light. Results are presented for the response of dissolved inorganic carbon (C1) flux rates to external C, in low-C1 grown cells of both Synechococcus PCC7942 and Chlamydomonas reinhardtii and these are consistent with previous studies showing that such cells possess capacities to utilize both CO2 and HCO−3 for photosynthesis. The advantages and potential errors which are inherent in this technique are discussed together with its potential for future studies on C1 transport under various experimental conditions. | ||
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10.1111/j.1399-3054.1994.tb08811.x doi (DE-627)NLEJ240943112 DE-627 ger DE-627 rakwb Badger, Murray R. verfasserin aut Measurement of CO2 and HCO3− fluxes in cyanobacteria and microalgae during steady-state photosynthesis Oxford, UK Blackwell Publishing Ltd 1994 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A mass spectrometric procedure is described which allows the simultaneous estimation of both CO2 and HCO−3 fluxes associated with cyanobacteria and green algae during steady-stale photosynthesis. This technique utilizes the chemical disequilibrium which exists between CO2 and HCO−3 during photosynthesis in cell suspensions which lack carbonic anhydrase activity. The kinetic equations which are derived for flux determinations are based on models of photosynthesis in both cyanobacteria and green algae which seem most reasonable given our present level of understanding, together with direct measurement of [CO2] estimation of [HCO−3] and application of the kinetic rate constants for the interconversion of CO2 and HCO−3 From measurements made in the light, net uptake of both CO2 and HCO−3 can be readily determined. In addition, analysis of the dark phase immediately following light-off provides the possibility of also determining the CO2 evolution whích ís occurring during photosynthesis, and thus also the gross CO2 uptake rates in the light. Results are presented for the response of dissolved inorganic carbon (C1) flux rates to external C, in low-C1 grown cells of both Synechococcus PCC7942 and Chlamydomonas reinhardtii and these are consistent with previous studies showing that such cells possess capacities to utilize both CO2 and HCO−3 for photosynthesis. The advantages and potential errors which are inherent in this technique are discussed together with its potential for future studies on C1 transport under various experimental conditions. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Cyanobacteria Palmqvist, Kristin verfasserin aut Yu, Jian-Wei verfasserin aut In Physiologia plantarum Oxford [u.a.] : Wiley-Blackwell, 1948 90(1994), 3, Seite 0 Online-Ressource (DE-627)NLEJ243927738 (DE-600)2020837-6 1399-3054 nnns volume:90 year:1994 number:3 pages:0 http://dx.doi.org/10.1111/j.1399-3054.1994.tb08811.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 90 1994 3 0 |
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10.1111/j.1399-3054.1994.tb08811.x doi (DE-627)NLEJ240943112 DE-627 ger DE-627 rakwb Badger, Murray R. verfasserin aut Measurement of CO2 and HCO3− fluxes in cyanobacteria and microalgae during steady-state photosynthesis Oxford, UK Blackwell Publishing Ltd 1994 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A mass spectrometric procedure is described which allows the simultaneous estimation of both CO2 and HCO−3 fluxes associated with cyanobacteria and green algae during steady-stale photosynthesis. This technique utilizes the chemical disequilibrium which exists between CO2 and HCO−3 during photosynthesis in cell suspensions which lack carbonic anhydrase activity. The kinetic equations which are derived for flux determinations are based on models of photosynthesis in both cyanobacteria and green algae which seem most reasonable given our present level of understanding, together with direct measurement of [CO2] estimation of [HCO−3] and application of the kinetic rate constants for the interconversion of CO2 and HCO−3 From measurements made in the light, net uptake of both CO2 and HCO−3 can be readily determined. In addition, analysis of the dark phase immediately following light-off provides the possibility of also determining the CO2 evolution whích ís occurring during photosynthesis, and thus also the gross CO2 uptake rates in the light. Results are presented for the response of dissolved inorganic carbon (C1) flux rates to external C, in low-C1 grown cells of both Synechococcus PCC7942 and Chlamydomonas reinhardtii and these are consistent with previous studies showing that such cells possess capacities to utilize both CO2 and HCO−3 for photosynthesis. The advantages and potential errors which are inherent in this technique are discussed together with its potential for future studies on C1 transport under various experimental conditions. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Cyanobacteria Palmqvist, Kristin verfasserin aut Yu, Jian-Wei verfasserin aut In Physiologia plantarum Oxford [u.a.] : Wiley-Blackwell, 1948 90(1994), 3, Seite 0 Online-Ressource (DE-627)NLEJ243927738 (DE-600)2020837-6 1399-3054 nnns volume:90 year:1994 number:3 pages:0 http://dx.doi.org/10.1111/j.1399-3054.1994.tb08811.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 90 1994 3 0 |
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10.1111/j.1399-3054.1994.tb08811.x doi (DE-627)NLEJ240943112 DE-627 ger DE-627 rakwb Badger, Murray R. verfasserin aut Measurement of CO2 and HCO3− fluxes in cyanobacteria and microalgae during steady-state photosynthesis Oxford, UK Blackwell Publishing Ltd 1994 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A mass spectrometric procedure is described which allows the simultaneous estimation of both CO2 and HCO−3 fluxes associated with cyanobacteria and green algae during steady-stale photosynthesis. This technique utilizes the chemical disequilibrium which exists between CO2 and HCO−3 during photosynthesis in cell suspensions which lack carbonic anhydrase activity. The kinetic equations which are derived for flux determinations are based on models of photosynthesis in both cyanobacteria and green algae which seem most reasonable given our present level of understanding, together with direct measurement of [CO2] estimation of [HCO−3] and application of the kinetic rate constants for the interconversion of CO2 and HCO−3 From measurements made in the light, net uptake of both CO2 and HCO−3 can be readily determined. In addition, analysis of the dark phase immediately following light-off provides the possibility of also determining the CO2 evolution whích ís occurring during photosynthesis, and thus also the gross CO2 uptake rates in the light. Results are presented for the response of dissolved inorganic carbon (C1) flux rates to external C, in low-C1 grown cells of both Synechococcus PCC7942 and Chlamydomonas reinhardtii and these are consistent with previous studies showing that such cells possess capacities to utilize both CO2 and HCO−3 for photosynthesis. The advantages and potential errors which are inherent in this technique are discussed together with its potential for future studies on C1 transport under various experimental conditions. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Cyanobacteria Palmqvist, Kristin verfasserin aut Yu, Jian-Wei verfasserin aut In Physiologia plantarum Oxford [u.a.] : Wiley-Blackwell, 1948 90(1994), 3, Seite 0 Online-Ressource (DE-627)NLEJ243927738 (DE-600)2020837-6 1399-3054 nnns volume:90 year:1994 number:3 pages:0 http://dx.doi.org/10.1111/j.1399-3054.1994.tb08811.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 90 1994 3 0 |
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10.1111/j.1399-3054.1994.tb08811.x doi (DE-627)NLEJ240943112 DE-627 ger DE-627 rakwb Badger, Murray R. verfasserin aut Measurement of CO2 and HCO3− fluxes in cyanobacteria and microalgae during steady-state photosynthesis Oxford, UK Blackwell Publishing Ltd 1994 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A mass spectrometric procedure is described which allows the simultaneous estimation of both CO2 and HCO−3 fluxes associated with cyanobacteria and green algae during steady-stale photosynthesis. This technique utilizes the chemical disequilibrium which exists between CO2 and HCO−3 during photosynthesis in cell suspensions which lack carbonic anhydrase activity. The kinetic equations which are derived for flux determinations are based on models of photosynthesis in both cyanobacteria and green algae which seem most reasonable given our present level of understanding, together with direct measurement of [CO2] estimation of [HCO−3] and application of the kinetic rate constants for the interconversion of CO2 and HCO−3 From measurements made in the light, net uptake of both CO2 and HCO−3 can be readily determined. In addition, analysis of the dark phase immediately following light-off provides the possibility of also determining the CO2 evolution whích ís occurring during photosynthesis, and thus also the gross CO2 uptake rates in the light. Results are presented for the response of dissolved inorganic carbon (C1) flux rates to external C, in low-C1 grown cells of both Synechococcus PCC7942 and Chlamydomonas reinhardtii and these are consistent with previous studies showing that such cells possess capacities to utilize both CO2 and HCO−3 for photosynthesis. The advantages and potential errors which are inherent in this technique are discussed together with its potential for future studies on C1 transport under various experimental conditions. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Cyanobacteria Palmqvist, Kristin verfasserin aut Yu, Jian-Wei verfasserin aut In Physiologia plantarum Oxford [u.a.] : Wiley-Blackwell, 1948 90(1994), 3, Seite 0 Online-Ressource (DE-627)NLEJ243927738 (DE-600)2020837-6 1399-3054 nnns volume:90 year:1994 number:3 pages:0 http://dx.doi.org/10.1111/j.1399-3054.1994.tb08811.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 90 1994 3 0 |
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10.1111/j.1399-3054.1994.tb08811.x doi (DE-627)NLEJ240943112 DE-627 ger DE-627 rakwb Badger, Murray R. verfasserin aut Measurement of CO2 and HCO3− fluxes in cyanobacteria and microalgae during steady-state photosynthesis Oxford, UK Blackwell Publishing Ltd 1994 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A mass spectrometric procedure is described which allows the simultaneous estimation of both CO2 and HCO−3 fluxes associated with cyanobacteria and green algae during steady-stale photosynthesis. This technique utilizes the chemical disequilibrium which exists between CO2 and HCO−3 during photosynthesis in cell suspensions which lack carbonic anhydrase activity. The kinetic equations which are derived for flux determinations are based on models of photosynthesis in both cyanobacteria and green algae which seem most reasonable given our present level of understanding, together with direct measurement of [CO2] estimation of [HCO−3] and application of the kinetic rate constants for the interconversion of CO2 and HCO−3 From measurements made in the light, net uptake of both CO2 and HCO−3 can be readily determined. In addition, analysis of the dark phase immediately following light-off provides the possibility of also determining the CO2 evolution whích ís occurring during photosynthesis, and thus also the gross CO2 uptake rates in the light. Results are presented for the response of dissolved inorganic carbon (C1) flux rates to external C, in low-C1 grown cells of both Synechococcus PCC7942 and Chlamydomonas reinhardtii and these are consistent with previous studies showing that such cells possess capacities to utilize both CO2 and HCO−3 for photosynthesis. The advantages and potential errors which are inherent in this technique are discussed together with its potential for future studies on C1 transport under various experimental conditions. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Cyanobacteria Palmqvist, Kristin verfasserin aut Yu, Jian-Wei verfasserin aut In Physiologia plantarum Oxford [u.a.] : Wiley-Blackwell, 1948 90(1994), 3, Seite 0 Online-Ressource (DE-627)NLEJ243927738 (DE-600)2020837-6 1399-3054 nnns volume:90 year:1994 number:3 pages:0 http://dx.doi.org/10.1111/j.1399-3054.1994.tb08811.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 90 1994 3 0 |
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Measurement of CO2 and HCO3− fluxes in cyanobacteria and microalgae during steady-state photosynthesis |
| abstract |
A mass spectrometric procedure is described which allows the simultaneous estimation of both CO2 and HCO−3 fluxes associated with cyanobacteria and green algae during steady-stale photosynthesis. This technique utilizes the chemical disequilibrium which exists between CO2 and HCO−3 during photosynthesis in cell suspensions which lack carbonic anhydrase activity. The kinetic equations which are derived for flux determinations are based on models of photosynthesis in both cyanobacteria and green algae which seem most reasonable given our present level of understanding, together with direct measurement of [CO2] estimation of [HCO−3] and application of the kinetic rate constants for the interconversion of CO2 and HCO−3 From measurements made in the light, net uptake of both CO2 and HCO−3 can be readily determined. In addition, analysis of the dark phase immediately following light-off provides the possibility of also determining the CO2 evolution whích ís occurring during photosynthesis, and thus also the gross CO2 uptake rates in the light. Results are presented for the response of dissolved inorganic carbon (C1) flux rates to external C, in low-C1 grown cells of both Synechococcus PCC7942 and Chlamydomonas reinhardtii and these are consistent with previous studies showing that such cells possess capacities to utilize both CO2 and HCO−3 for photosynthesis. The advantages and potential errors which are inherent in this technique are discussed together with its potential for future studies on C1 transport under various experimental conditions. |
| abstractGer |
A mass spectrometric procedure is described which allows the simultaneous estimation of both CO2 and HCO−3 fluxes associated with cyanobacteria and green algae during steady-stale photosynthesis. This technique utilizes the chemical disequilibrium which exists between CO2 and HCO−3 during photosynthesis in cell suspensions which lack carbonic anhydrase activity. The kinetic equations which are derived for flux determinations are based on models of photosynthesis in both cyanobacteria and green algae which seem most reasonable given our present level of understanding, together with direct measurement of [CO2] estimation of [HCO−3] and application of the kinetic rate constants for the interconversion of CO2 and HCO−3 From measurements made in the light, net uptake of both CO2 and HCO−3 can be readily determined. In addition, analysis of the dark phase immediately following light-off provides the possibility of also determining the CO2 evolution whích ís occurring during photosynthesis, and thus also the gross CO2 uptake rates in the light. Results are presented for the response of dissolved inorganic carbon (C1) flux rates to external C, in low-C1 grown cells of both Synechococcus PCC7942 and Chlamydomonas reinhardtii and these are consistent with previous studies showing that such cells possess capacities to utilize both CO2 and HCO−3 for photosynthesis. The advantages and potential errors which are inherent in this technique are discussed together with its potential for future studies on C1 transport under various experimental conditions. |
| abstract_unstemmed |
A mass spectrometric procedure is described which allows the simultaneous estimation of both CO2 and HCO−3 fluxes associated with cyanobacteria and green algae during steady-stale photosynthesis. This technique utilizes the chemical disequilibrium which exists between CO2 and HCO−3 during photosynthesis in cell suspensions which lack carbonic anhydrase activity. The kinetic equations which are derived for flux determinations are based on models of photosynthesis in both cyanobacteria and green algae which seem most reasonable given our present level of understanding, together with direct measurement of [CO2] estimation of [HCO−3] and application of the kinetic rate constants for the interconversion of CO2 and HCO−3 From measurements made in the light, net uptake of both CO2 and HCO−3 can be readily determined. In addition, analysis of the dark phase immediately following light-off provides the possibility of also determining the CO2 evolution whích ís occurring during photosynthesis, and thus also the gross CO2 uptake rates in the light. Results are presented for the response of dissolved inorganic carbon (C1) flux rates to external C, in low-C1 grown cells of both Synechococcus PCC7942 and Chlamydomonas reinhardtii and these are consistent with previous studies showing that such cells possess capacities to utilize both CO2 and HCO−3 for photosynthesis. The advantages and potential errors which are inherent in this technique are discussed together with its potential for future studies on C1 transport under various experimental conditions. |
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| title_short |
Measurement of CO2 and HCO3− fluxes in cyanobacteria and microalgae during steady-state photosynthesis |
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Palmqvist, Kristin Yu, Jian-Wei |
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10.1111/j.1399-3054.1994.tb08811.x |
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