Endonuclease activity from tobacco nuclei specific for ultraviolet radiation-damaged DNA
Endonuclease activity specific for UV damaged DNA was isolated from tobacco leaf nuclei and detected by relaxation of supercoiled pUC 19 plasmid DNA. The activity did not require divalent cations or ATP. It acted on photoproducts induced by as little as 24 J m−2 of UV-C (primarily 254 nm) radiation....
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Murphy, Terence M. [verfasserIn] Martin, Charles P. [verfasserIn] Kami, James [verfasserIn] |
|---|
| Format: |
E-Artikel |
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| Erschienen: |
Oxford, UK: Blackwell Publishing Ltd ; 1993 |
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| Schlagwörter: |
|---|
| Umfang: |
Online-Ressource |
|---|
| Reproduktion: |
2006 ; Blackwell Publishing Journal Backfiles 1879-2005 |
|---|---|
| Übergeordnetes Werk: |
In: Physiologia plantarum - Oxford [u.a.] : Wiley-Blackwell, 1948, 87(1993), 3, Seite 0 |
| Übergeordnetes Werk: |
volume:87 ; year:1993 ; number:3 ; pages:0 |
| Links: |
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| DOI / URN: |
10.1111/j.1399-3054.1993.tb01750.x |
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| 520 | |a Endonuclease activity specific for UV damaged DNA was isolated from tobacco leaf nuclei and detected by relaxation of supercoiled pUC 19 plasmid DNA. The activity did not require divalent cations or ATP. It acted on photoproducts induced by as little as 24 J m−2 of UV-C (primarily 254 nm) radiation. but not on photoproducts produced by UV-B (290–320 nm) radiation in the presence of acetophenone and a N2 atmosphere or by UV-A (320–400 nm) radiation in the presence of 4′-methoxy-methyltrioxsalen in a N2 atmosphere and not on the products of OsO4 oxidation of the DNA. Using end-labeled DNA of defined sequence, it was possible to identify sites in UV-C-irradiated DNA that were cut by the endonuclease preparation: most sites were assocrated with pyrimidine pairs. Cleavage by the tobacco endonuclease was not eliminated by treatment with Escherichia coli photolyase and light, suggesting that the endonuclease did not recognize cyclobutadipyrimidines. | ||
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10.1111/j.1399-3054.1993.tb01750.x doi (DE-627)NLEJ240947193 DE-627 ger DE-627 rakwb Murphy, Terence M. verfasserin aut Endonuclease activity from tobacco nuclei specific for ultraviolet radiation-damaged DNA Oxford, UK Blackwell Publishing Ltd 1993 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Endonuclease activity specific for UV damaged DNA was isolated from tobacco leaf nuclei and detected by relaxation of supercoiled pUC 19 plasmid DNA. The activity did not require divalent cations or ATP. It acted on photoproducts induced by as little as 24 J m−2 of UV-C (primarily 254 nm) radiation. but not on photoproducts produced by UV-B (290–320 nm) radiation in the presence of acetophenone and a N2 atmosphere or by UV-A (320–400 nm) radiation in the presence of 4′-methoxy-methyltrioxsalen in a N2 atmosphere and not on the products of OsO4 oxidation of the DNA. Using end-labeled DNA of defined sequence, it was possible to identify sites in UV-C-irradiated DNA that were cut by the endonuclease preparation: most sites were assocrated with pyrimidine pairs. Cleavage by the tobacco endonuclease was not eliminated by treatment with Escherichia coli photolyase and light, suggesting that the endonuclease did not recognize cyclobutadipyrimidines. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| DNA damage. DNA endonuclease. DNA repair. Martin, Charles P. verfasserin aut Kami, James verfasserin aut In Physiologia plantarum Oxford [u.a.] : Wiley-Blackwell, 1948 87(1993), 3, Seite 0 Online-Ressource (DE-627)NLEJ243927738 (DE-600)2020837-6 1399-3054 nnns volume:87 year:1993 number:3 pages:0 http://dx.doi.org/10.1111/j.1399-3054.1993.tb01750.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 87 1993 3 0 |
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10.1111/j.1399-3054.1993.tb01750.x doi (DE-627)NLEJ240947193 DE-627 ger DE-627 rakwb Murphy, Terence M. verfasserin aut Endonuclease activity from tobacco nuclei specific for ultraviolet radiation-damaged DNA Oxford, UK Blackwell Publishing Ltd 1993 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Endonuclease activity specific for UV damaged DNA was isolated from tobacco leaf nuclei and detected by relaxation of supercoiled pUC 19 plasmid DNA. The activity did not require divalent cations or ATP. It acted on photoproducts induced by as little as 24 J m−2 of UV-C (primarily 254 nm) radiation. but not on photoproducts produced by UV-B (290–320 nm) radiation in the presence of acetophenone and a N2 atmosphere or by UV-A (320–400 nm) radiation in the presence of 4′-methoxy-methyltrioxsalen in a N2 atmosphere and not on the products of OsO4 oxidation of the DNA. Using end-labeled DNA of defined sequence, it was possible to identify sites in UV-C-irradiated DNA that were cut by the endonuclease preparation: most sites were assocrated with pyrimidine pairs. Cleavage by the tobacco endonuclease was not eliminated by treatment with Escherichia coli photolyase and light, suggesting that the endonuclease did not recognize cyclobutadipyrimidines. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| DNA damage. DNA endonuclease. DNA repair. Martin, Charles P. verfasserin aut Kami, James verfasserin aut In Physiologia plantarum Oxford [u.a.] : Wiley-Blackwell, 1948 87(1993), 3, Seite 0 Online-Ressource (DE-627)NLEJ243927738 (DE-600)2020837-6 1399-3054 nnns volume:87 year:1993 number:3 pages:0 http://dx.doi.org/10.1111/j.1399-3054.1993.tb01750.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 87 1993 3 0 |
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10.1111/j.1399-3054.1993.tb01750.x doi (DE-627)NLEJ240947193 DE-627 ger DE-627 rakwb Murphy, Terence M. verfasserin aut Endonuclease activity from tobacco nuclei specific for ultraviolet radiation-damaged DNA Oxford, UK Blackwell Publishing Ltd 1993 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Endonuclease activity specific for UV damaged DNA was isolated from tobacco leaf nuclei and detected by relaxation of supercoiled pUC 19 plasmid DNA. The activity did not require divalent cations or ATP. It acted on photoproducts induced by as little as 24 J m−2 of UV-C (primarily 254 nm) radiation. but not on photoproducts produced by UV-B (290–320 nm) radiation in the presence of acetophenone and a N2 atmosphere or by UV-A (320–400 nm) radiation in the presence of 4′-methoxy-methyltrioxsalen in a N2 atmosphere and not on the products of OsO4 oxidation of the DNA. Using end-labeled DNA of defined sequence, it was possible to identify sites in UV-C-irradiated DNA that were cut by the endonuclease preparation: most sites were assocrated with pyrimidine pairs. Cleavage by the tobacco endonuclease was not eliminated by treatment with Escherichia coli photolyase and light, suggesting that the endonuclease did not recognize cyclobutadipyrimidines. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| DNA damage. DNA endonuclease. DNA repair. Martin, Charles P. verfasserin aut Kami, James verfasserin aut In Physiologia plantarum Oxford [u.a.] : Wiley-Blackwell, 1948 87(1993), 3, Seite 0 Online-Ressource (DE-627)NLEJ243927738 (DE-600)2020837-6 1399-3054 nnns volume:87 year:1993 number:3 pages:0 http://dx.doi.org/10.1111/j.1399-3054.1993.tb01750.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 87 1993 3 0 |
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10.1111/j.1399-3054.1993.tb01750.x doi (DE-627)NLEJ240947193 DE-627 ger DE-627 rakwb Murphy, Terence M. verfasserin aut Endonuclease activity from tobacco nuclei specific for ultraviolet radiation-damaged DNA Oxford, UK Blackwell Publishing Ltd 1993 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Endonuclease activity specific for UV damaged DNA was isolated from tobacco leaf nuclei and detected by relaxation of supercoiled pUC 19 plasmid DNA. The activity did not require divalent cations or ATP. It acted on photoproducts induced by as little as 24 J m−2 of UV-C (primarily 254 nm) radiation. but not on photoproducts produced by UV-B (290–320 nm) radiation in the presence of acetophenone and a N2 atmosphere or by UV-A (320–400 nm) radiation in the presence of 4′-methoxy-methyltrioxsalen in a N2 atmosphere and not on the products of OsO4 oxidation of the DNA. Using end-labeled DNA of defined sequence, it was possible to identify sites in UV-C-irradiated DNA that were cut by the endonuclease preparation: most sites were assocrated with pyrimidine pairs. Cleavage by the tobacco endonuclease was not eliminated by treatment with Escherichia coli photolyase and light, suggesting that the endonuclease did not recognize cyclobutadipyrimidines. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| DNA damage. DNA endonuclease. DNA repair. Martin, Charles P. verfasserin aut Kami, James verfasserin aut In Physiologia plantarum Oxford [u.a.] : Wiley-Blackwell, 1948 87(1993), 3, Seite 0 Online-Ressource (DE-627)NLEJ243927738 (DE-600)2020837-6 1399-3054 nnns volume:87 year:1993 number:3 pages:0 http://dx.doi.org/10.1111/j.1399-3054.1993.tb01750.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 87 1993 3 0 |
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10.1111/j.1399-3054.1993.tb01750.x doi (DE-627)NLEJ240947193 DE-627 ger DE-627 rakwb Murphy, Terence M. verfasserin aut Endonuclease activity from tobacco nuclei specific for ultraviolet radiation-damaged DNA Oxford, UK Blackwell Publishing Ltd 1993 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Endonuclease activity specific for UV damaged DNA was isolated from tobacco leaf nuclei and detected by relaxation of supercoiled pUC 19 plasmid DNA. The activity did not require divalent cations or ATP. It acted on photoproducts induced by as little as 24 J m−2 of UV-C (primarily 254 nm) radiation. but not on photoproducts produced by UV-B (290–320 nm) radiation in the presence of acetophenone and a N2 atmosphere or by UV-A (320–400 nm) radiation in the presence of 4′-methoxy-methyltrioxsalen in a N2 atmosphere and not on the products of OsO4 oxidation of the DNA. Using end-labeled DNA of defined sequence, it was possible to identify sites in UV-C-irradiated DNA that were cut by the endonuclease preparation: most sites were assocrated with pyrimidine pairs. Cleavage by the tobacco endonuclease was not eliminated by treatment with Escherichia coli photolyase and light, suggesting that the endonuclease did not recognize cyclobutadipyrimidines. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| DNA damage. DNA endonuclease. DNA repair. Martin, Charles P. verfasserin aut Kami, James verfasserin aut In Physiologia plantarum Oxford [u.a.] : Wiley-Blackwell, 1948 87(1993), 3, Seite 0 Online-Ressource (DE-627)NLEJ243927738 (DE-600)2020837-6 1399-3054 nnns volume:87 year:1993 number:3 pages:0 http://dx.doi.org/10.1111/j.1399-3054.1993.tb01750.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 87 1993 3 0 |
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Endonuclease activity from tobacco nuclei specific for ultraviolet radiation-damaged DNA DNA damage. DNA endonuclease. DNA repair |
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Endonuclease activity from tobacco nuclei specific for ultraviolet radiation-damaged DNA |
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Endonuclease activity specific for UV damaged DNA was isolated from tobacco leaf nuclei and detected by relaxation of supercoiled pUC 19 plasmid DNA. The activity did not require divalent cations or ATP. It acted on photoproducts induced by as little as 24 J m−2 of UV-C (primarily 254 nm) radiation. but not on photoproducts produced by UV-B (290–320 nm) radiation in the presence of acetophenone and a N2 atmosphere or by UV-A (320–400 nm) radiation in the presence of 4′-methoxy-methyltrioxsalen in a N2 atmosphere and not on the products of OsO4 oxidation of the DNA. Using end-labeled DNA of defined sequence, it was possible to identify sites in UV-C-irradiated DNA that were cut by the endonuclease preparation: most sites were assocrated with pyrimidine pairs. Cleavage by the tobacco endonuclease was not eliminated by treatment with Escherichia coli photolyase and light, suggesting that the endonuclease did not recognize cyclobutadipyrimidines. |
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Endonuclease activity specific for UV damaged DNA was isolated from tobacco leaf nuclei and detected by relaxation of supercoiled pUC 19 plasmid DNA. The activity did not require divalent cations or ATP. It acted on photoproducts induced by as little as 24 J m−2 of UV-C (primarily 254 nm) radiation. but not on photoproducts produced by UV-B (290–320 nm) radiation in the presence of acetophenone and a N2 atmosphere or by UV-A (320–400 nm) radiation in the presence of 4′-methoxy-methyltrioxsalen in a N2 atmosphere and not on the products of OsO4 oxidation of the DNA. Using end-labeled DNA of defined sequence, it was possible to identify sites in UV-C-irradiated DNA that were cut by the endonuclease preparation: most sites were assocrated with pyrimidine pairs. Cleavage by the tobacco endonuclease was not eliminated by treatment with Escherichia coli photolyase and light, suggesting that the endonuclease did not recognize cyclobutadipyrimidines. |
| abstract_unstemmed |
Endonuclease activity specific for UV damaged DNA was isolated from tobacco leaf nuclei and detected by relaxation of supercoiled pUC 19 plasmid DNA. The activity did not require divalent cations or ATP. It acted on photoproducts induced by as little as 24 J m−2 of UV-C (primarily 254 nm) radiation. but not on photoproducts produced by UV-B (290–320 nm) radiation in the presence of acetophenone and a N2 atmosphere or by UV-A (320–400 nm) radiation in the presence of 4′-methoxy-methyltrioxsalen in a N2 atmosphere and not on the products of OsO4 oxidation of the DNA. Using end-labeled DNA of defined sequence, it was possible to identify sites in UV-C-irradiated DNA that were cut by the endonuclease preparation: most sites were assocrated with pyrimidine pairs. Cleavage by the tobacco endonuclease was not eliminated by treatment with Escherichia coli photolyase and light, suggesting that the endonuclease did not recognize cyclobutadipyrimidines. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ240947193</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210707123040.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">120426s1993 xx |||||o 00| ||und c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1111/j.1399-3054.1993.tb01750.x</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ240947193</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Murphy, Terence M.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Endonuclease activity from tobacco nuclei specific for ultraviolet radiation-damaged DNA</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Oxford, UK</subfield><subfield code="b">Blackwell Publishing Ltd</subfield><subfield code="c">1993</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Endonuclease activity specific for UV damaged DNA was isolated from tobacco leaf nuclei and detected by relaxation of supercoiled pUC 19 plasmid DNA. The activity did not require divalent cations or ATP. It acted on photoproducts induced by as little as 24 J m−2 of UV-C (primarily 254 nm) radiation. but not on photoproducts produced by UV-B (290–320 nm) radiation in the presence of acetophenone and a N2 atmosphere or by UV-A (320–400 nm) radiation in the presence of 4′-methoxy-methyltrioxsalen in a N2 atmosphere and not on the products of OsO4 oxidation of the DNA. Using end-labeled DNA of defined sequence, it was possible to identify sites in UV-C-irradiated DNA that were cut by the endonuclease preparation: most sites were assocrated with pyrimidine pairs. Cleavage by the tobacco endonuclease was not eliminated by treatment with Escherichia coli photolyase and light, suggesting that the endonuclease did not recognize cyclobutadipyrimidines.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="d">2006</subfield><subfield code="f">Blackwell Publishing Journal Backfiles 1879-2005</subfield><subfield code="7">|2006||||||||||</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">DNA damage. DNA endonuclease. DNA repair.</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Martin, Charles P.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Kami, James</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Physiologia plantarum</subfield><subfield code="d">Oxford [u.a.] : Wiley-Blackwell, 1948</subfield><subfield code="g">87(1993), 3, Seite 0</subfield><subfield code="h">Online-Ressource</subfield><subfield code="w">(DE-627)NLEJ243927738</subfield><subfield code="w">(DE-600)2020837-6</subfield><subfield code="x">1399-3054</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:87</subfield><subfield code="g">year:1993</subfield><subfield code="g">number:3</subfield><subfield code="g">pages:0</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1111/j.1399-3054.1993.tb01750.x</subfield><subfield code="q">text/html</subfield><subfield code="x">Verlag</subfield><subfield code="z">Deutschlandweit zugänglich</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-DJB</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">87</subfield><subfield code="j">1993</subfield><subfield code="e">3</subfield><subfield code="h">0</subfield></datafield></record></collection>
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